DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment NAT1 and eif4g2b

DIOPT Version :9

Sequence 1:	NP_001033943.2	Gene:	NAT1 / 46020	FlyBaseID:	FBgn0010488	Length:	1488	Species:	Drosophila melanogaster
Sequence 2:	NP_001013461.2	Gene:	eif4g2b / 334618	ZFINID:	ZDB-GENE-030131-6550	Length:	898	Species:	Danio rerio

Alignment Length:	1056	Identity:	365/1056 - (34%)
Similarity:	538/1056 - (50%)	Gaps:	205/1056 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly   464 SASAAGGGTPHSQQQQERSQAGK---ASTPPPITGRWIP--PSLRPQHGLTQSEKNDAVFRRVRG 523
            |||:.|||...:.|...::....   ..||.|...||:|  .:.|..:..|:.|.:||:||:|||
Zfish    14 SASSGGGGGRGASQHYPKTVGNSEYLGKTPGPSVQRWVPSRSTRRDVNSSTEKEHHDAIFRKVRG 78

  Fly   524 ILNKLTPEKFQELSDELLKLDLNSIAILKGVILLIFDKALDEPKYSSMYAQLCKRLSEEAPSFD- 587
            ||||||||||.:|..|||.:.::|..:|||:||||.||||:||||||:|||||.||:|:||:||
Zfish    79 ILNKLTPEKFDKLCLELLNVGVDSKLVLKGIILLIVDKALEEPKYSSLYAQLCLRLAEDAPNFDG 143

  Fly   588 ------KEPSNSSTFLRLLIAVCRDKFNNRLKR----DENDNRPPPENEADEEERRHLAKQRMLG 642
                  .....|:||.||||...:|:|.||.|.    |:.||  |..:|  |||:|.:||.:|||
Zfish   144 PTPEIQSSQKQSTTFRRLLITKLQDEFENRTKNVDLYDKQDN--PLTSE--EEEQRAIAKIKMLG 204

  Fly   643 NVKFIGELNKLDMLSKNVLHQCIMELFDKKKKRTAGTQEMCEDMECLAQLLKTCGKNLDSEQGKE 707
            |:||||||.|||::.:::||:||..|.:|||:  ...::|.||:|||.|:::|.|..||.|:.|.
Zfish   205 NIKFIGELGKLDLIHESILHKCIKTLLEKKKR--VQLKDMGEDLECLCQIMRTVGPRLDHEKAKS 267

  Fly   708 LMNQYFEKLERRSKSSEYPPRIRFMLKDVIELRQNNWVPRKLGTTEGPVPIKQIRSDDDSIIRTP 772
            ||:|||.:::....:.:.|.||||:|:|.:|||:|||||||.....||..|.|||.|....:...
Zfish   268 LMDQYFGRMQSLMNNKDLPARIRFLLQDTVELRENNWVPRKAFIDNGPKTINQIRQDAVKDLGVF 332

  Fly   773 FPNRNRDMRNNDRDSDSWM-NRFHLNIQP-GGYNDMF-----SGLSVTGASPIVSPFATSNRDRD 830
            .|..|:.||.:......:| ||..|:.:. ||..|||     ||:. ||...|...|:.: ..|.
Zfish   333 IPPMNQGMRMDFFLEGPFMPNRMKLDRETLGGLADMFGQMPGSGIG-TGPGVIQDRFSPT-LGRH 395

  Fly   831 RDNRQYNNRGDRNRDRDQGGSG-----GANYGNRYNKHNQNGGGSGGGSSNNRDRDDSRRNNDRD 890
            |.|..:|.. ..::.:.|...|     .:|.|...:.||||        .|:.::..::
Zfish   396 RTNPLFNGH-MASQPQSQFDLGMKPFMKSNQGQNQHFHNQN--------QNHLNQQQAQ------ 445

  Fly   891 RDRNDGQYGGNQLLGSKELAPRFKRNLITTNQDAVENLQMRPAANSLLFRAASQNQ--KFPATLP 953
                           ||::.||||:     .|...:.:.:|||.:.||    ::||  |....:|
Zfish   446 ---------------SKDMPPRFKK-----GQMNADEISLRPAQSFLL----NKNQVPKLQPQIP 486

  Fly   954 MSTPPNSSGSNNQGSSQGQLSNSHYPMLGTPTSHLINPTRPSSTPSAEYADNRTLLGVQQERGLK 1018
            ...||::.....|....||     .|.||..|    ||.                  :.||:..|
Zfish   487 TMIPPSAQPPRTQTPPLGQ-----PPQLGLKT----NPP------------------LIQEKPQK 524

  Fly  1019 ATSSSPNFSSGAEKLDLDGQAGRKGSHDGKDQGKNGSVERPSTPAGGIGSAKQQKKDKGPNKEEL 1083
            .|...|                                                     |.:|||
Zfish   525 TTKKPP-----------------------------------------------------PAREEL 536

  Fly  1084 SKKASQYFKDRFYYDEEEDAEVTEIEIKDIDETKTEEADAAPVEVSEVKEDAAIPVTTPDRFTKL 1148
                                      :|..:...||..:...:||:
Zfish   537 --------------------------LKMTENIVTEYLNNRNMEVA------------------- 556

  Fly  1149 VDGFLELKLPEKALKDVCINLLMEVLDRVNDVFLERAVRLLQTLRKQSNIKPNIVVEIFKQVVNK 1213
            |.|..|:|.|:..|.::...:::..|:| .|...|:|..|:.|||.:..|.....::....|:::
Zfish   557 VSGVREMKAPKHFLPEMLSKIILCSLER-TDEDREQASTLINTLRTEGFITGEHFMQALLNVLDQ 620

  Fly  1214 MNEREALNPRIVSLVASLLAKTICEPALLKLSDIANFTDNGQHYPLFLLVLQQLVKVIGKDALEE 1278
            ..:.|...|.:.|.:|...|:.:. ..|:.::::|:..:||.|:|||||.|||..|:..::.|.:
Zfish   621 CPKIEVDIPLVKSYLAQFAARAVI-AELVSVAELAHPLENGNHFPLFLLCLQQASKLKDREWLTD 684

  Fly  1279 KFRASKVDLMNSLPEADRNKERLAGILEDRKLSFLYPLLKVQAEMLKQLQSDPNPNNFYKWIKAN 1343
            .|:.|||::...|||.|:||:|:..:||.:.||||:||||::.|:|||:::||:|...|||||.|
Zfish   685 LFQQSKVNMQKMLPEIDQNKDRMLEVLEGKGLSFLFPLLKLEKELLKQIKADPSPQTIYKWIKDN 749

  Fly  1344 VDNKYYKDPGFIQALMTVVVKYVTKETTLADNLDPKEHPEKSVTQKEQQLLENYSQMLQTFLGQN 1408
            :..|.:.|.|||..|:|..::|:..|..:.:..:....|.|....:|:|||.::..::|.||.::
Zfish   750 ISPKLHIDKGFINILVTSFLQYIFAELGVPECDEQLSFPSKEQLDQEKQLLHSFKPVMQKFLHEH 814

  Fly  1409 -ELQLMALYALQTFCYNENFPKGMLCRWFKYLYESEIIEEEAFVLWKEEISDKYPGKGSALFQVN 1472
             .||:.||||||..|....||||||.|:|.:.|:.||||||||:.|||:||.::||||.||||||
Zfish   815 TNLQVSALYALQVHCNANAFPKGMLLRYFVHFYDMEIIEEEAFLAWKEDISQEFPGKGKALFQVN 879

  Fly  1473 AWLTWLQEAESEDDED 1488
            .|||||:.||.|:.|:
Zfish   880 QWLTWLETAEEEESEE 895

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
NAT1	NP_001033943.2	MIF4G	518..743	CDD:280935	121/235 (51%)
		RAM	<817..865	CDD:291966	11/52 (21%)
		MA3	1128..1241	CDD:214714	23/112 (21%)
		W2_eIF4G1_like	1314..1460	CDD:211397	66/146 (45%)
eif4g2b	NP_001013461.2	MIF4G	73..303	CDD:280935	121/235 (51%)
		MA3	536..649	CDD:214714	29/159 (18%)
		W2_eIF4G1_like	732..867	CDD:211397	59/134 (44%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170594707
Domainoid	1	1.000	231	1.000	Domainoid score	I2367
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H134127
Inparanoid	1	1.050	572	1.000	Inparanoid score	I1020
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D537108at33208
OrthoFinder	1	1.000	-	-		FOG0004422
OrthoInspector	1	1.000	-	-		otm25958
orthoMCL	1	0.900	-	-		OOG6_107094
Panther	1	1.100	-	-	O	PTHR23253
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X4221
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.900