DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment NAT1 and eif4g2a

DIOPT Version :9

Sequence 1:	NP_001033943.2	Gene:	NAT1 / 46020	FlyBaseID:	FBgn0010488	Length:	1488	Species:	Drosophila melanogaster
Sequence 2:	NP_001014311.1	Gene:	eif4g2a / 323453	ZFINID:	ZDB-GENE-030131-2173	Length:	891	Species:	Danio rerio

Alignment Length:	1063	Identity:	355/1063 - (33%)
Similarity:	527/1063 - (49%)	Gaps:	226/1063 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly   464 SASAAGGGTPHSQQQQERSQAGK---ASTPPPITGRWIP--PSLRPQHGLTQSEKNDAVFRRVRG 523
            |||:.|||...:.|...:|....   ..||.|...||:|  .:.|..:...:.|::||:||:|||
Zfish    14 SASSGGGGGRGATQHYPKSVGNSEFLGKTPGPSVQRWVPSRSTRRDVNSSNEKERHDAIFRKVRG 78

  Fly   524 ILNKLTPEKFQELSDELLKLDLNSIAILKGVILLIFDKALDEPKYSSMYAQLCKRLSEEAPSFD- 587
            ||||||||||.:|..|||.:.::|..:|||:||||.||||:||||||:|||||.||:|:||:||
Zfish    79 ILNKLTPEKFDKLCLELLNVGVDSKLVLKGIILLIVDKALEEPKYSSLYAQLCLRLAEDAPNFDG 143

  Fly   588 ------KEPSNSSTFLRLLIAVCRDKFNNRLKR----DENDNRPPPENEADEEERRHLAKQRMLG 642
                  .....|:||.||||:..:|:|.||.:.    |:||:  |..:|  |||:|.:||.:|||
Zfish   144 PSTEIQSSQKQSTTFRRLLISKLQDEFENRTRNVDIYDKNDS--PLTSE--EEEQRAIAKIKMLG 204

  Fly   643 NVKFIGELNKLDMLSKNVLHQCIMELFDKKKKRTAGTQEMCEDMECLAQLLKTCGKNLDSEQGKE 707
            |:||||||.|||::.:::||:||..|.:|||:  ...::|.||:|||.|:::|.|..||.|:.|.
Zfish   205 NIKFIGELGKLDLIHESILHKCIKTLLEKKKR--VQLKDMGEDLECLCQIMRTVGPRLDHEKAKS 267

  Fly   708 LMNQYFEKLERRSKSSEYPPRIRFMLKDVIELRQNNWVPRKLGTTEGPVPIKQIRSDDDSIIRTP 772
            ||:|||.::.....:.:.|.||||:|:|.:|||:|||||||.....||..|.|||.|....:...
Zfish   268 LMDQYFGRMRSLMNNKDLPARIRFLLQDTVELRENNWVPRKAFIDNGPKTINQIRQDAVKDLGVF 332

  Fly   773 FPNRNRDMRNNDRDSDSWMNRFHLNIQP-----GGYNDMF-----SGLSVTGASPIVSPFA-TSN 826
            .|...:.:|.:....:|:|   ...|:|     ||..|||     ||:. ||...|...:: |..
Zfish   333 IPPMTQGIRTDFFQENSFM---PTRIKPDRETLGGLADMFGQMPGSGIG-TGPGVIQDRYSPTMG 393

  Fly   827 RDRDRDNRQYNNRGDRNRDRDQGGSG----------GANYGNRYNKHNQNGGGSGGGSSNNRDRD 881
            |   |.|..:|           |.||          .:|.|......||.
Zfish   394 R---RTNPLFN-----------GHSGHMAPAPQPFIKSNQGQNLLFQNQT--------------- 429

  Fly   882 DSRRNNDRDRDRNDGQYGGNQLLGSKELAPRFKRNLITTNQDAVENLQMRPAANSLLFRAASQNQ 946
                            :...|...||::.|||.:.    .|...:.:.:|||.:.:|.:  :|..
Zfish   430 ----------------HSSQQQAQSKDMPPRFTKK----GQLNADEISLRPAQSFILNK--NQMP 472

  Fly   947 KFPATLPMSTPPNSSGSNNQGSSQGQLSNSHYPMLGTPTSHLINPTRPSSTPSAEYADNRTLLGV 1011
            |....:|...||::                             .|.|.|:.|          ||.
Zfish   473 KLQPQIPTMIPPSA-----------------------------QPPRTSTPP----------LGQ 498

  Fly  1012 QQERGLKATSSSPNFSSGAEKLDLDGQAGRKGSHDGKDQGKNGSVERPSTPAGGIGSAKQQKKDK 1076
            ..:.|||  ::.|......:|                      ::::|.
Zfish   499 PPQLGLK--TNPPPIQEKPQK----------------------TIKKPP---------------- 523

  Fly  1077 GPNKEELSKKASQYFKDRFYYDEEEDAEVTEIEIKDIDETKTEEADAAPVEVSEVKEDAAIPVTT 1141
             |.||||.|                   :||..:.....:|.             ..||
Zfish   524 -PAKEELLK-------------------MTETVVTSYLSSKN-------------MNDA------ 549

  Fly  1142 PDRFTKLVDGFLELKLPEKALKDVCINLLMEVLDRVNDVFLERAVRLLQTLRKQSNIKPNIVVEI 1206
                   |:|..|:|.|:..|.::...:::..|:. .|...|....|:..||.:..:.....::.
Zfish   550 -------VNGVREMKAPKHFLPEMLSKMIICSLEN-PDEDREHVSTLINKLRVEGLVSGENFLQA 606

  Fly  1207 FKQVVNKMNEREALNPRIVSLVASLLAKTICEPALLKLSDIANFTDNGQHYPLFLLVLQQLVKVI 1271
            |..|:::.::.|...|.:.|.:|...|:.:. ..|:.::::|:..:||.|:|||||.|||:.|:.
Zfish   607 FLNVLDQCSKIELDVPLVKSYLAQFAARAVI-AELVSMAELAHPLENGTHFPLFLLCLQQMAKLK 670

  Fly  1272 GKDALEEKFRASKVDLMNSLPEADRNKERLAGILEDRKLSFLYPLLKVQAEMLKQLQSDPNPNNF 1336
            .::.|.:.|:.|||::...|||.|:||:|:..|||.:.||||:||:|::.|:|||:::||.|...
Zfish   671 DREWLTDLFQQSKVNMQKMLPEIDQNKDRMLEILEGKGLSFLFPLMKLEKELLKQIKADPAPQTI 735

  Fly  1337 YKWIKANVDNKYYKDPGFIQALMTVVVKYVTKETTLADNLDPKEHPEKSVTQKEQQLLENYSQML 1401
            |||||.|:..|.:.|.||:..|||..::|:..|....|:.:....|.|....:|:|||..:..::
Zfish   736 YKWIKDNISPKLHTDRGFVNILMTSFLQYIFHEMYATDDEEQLSAPTKEQLDQEKQLLLAFKPVM 800

  Fly  1402 QTFLGQN-ELQLMALYALQTFCYNENFPKGMLCRWFKYLYESEIIEEEAFVLWKEEISDKYPGKG 1465
            |.||..: |||:.||||||..|....||||||.|:|...|:.||||||||:.|||:|:.::||||
Zfish   801 QKFLHDHVELQVSALYALQVHCNAHAFPKGMLLRYFVNFYDMEIIEEEAFLAWKEDITQEFPGKG 865

  Fly  1466 SALFQVNAWLTWLQEAESEDDED 1488
            .||||||.||.||:.||.|:.|:
Zfish   866 KALFQVNQWLMWLETAEEEESEE 888

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
NAT1	NP_001033943.2	MIF4G	518..743	CDD:280935	120/235 (51%)
		RAM	<817..865	CDD:291966	11/58 (19%)
		MA3	1128..1241	CDD:214714	21/112 (19%)
		W2_eIF4G1_like	1314..1460	CDD:211397	66/146 (45%)
eif4g2a	NP_001014311.1	MIF4G	73..303	CDD:280935	120/235 (51%)
		Med25_SD1	376..523	CDD:288132	43/261 (16%)
		MA3	529..642	CDD:214714	27/159 (17%)
		W2_eIF4G1_like	713..860	CDD:211397	66/146 (45%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170594706
Domainoid	1	1.000	231	1.000	Domainoid score	I2367
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H134127
Inparanoid	1	1.050	572	1.000	Inparanoid score	I1020
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D537108at33208
OrthoFinder	1	1.000	-	-		FOG0004422
OrthoInspector	1	1.000	-	-		otm25958
orthoMCL	1	0.900	-	-		OOG6_107094
Panther	1	1.100	-	-	O	PTHR23253
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X4221
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.900