DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment NAT1 and EIF4G1

DIOPT Version :9

Sequence 1:	NP_001033943.2	Gene:	NAT1 / 46020	FlyBaseID:	FBgn0010488	Length:	1488	Species:	Drosophila melanogaster
Sequence 2:	NP_001181875.2	Gene:	EIF4G1 / 1981	HGNCID:	3296	Length:	1606	Species:	Homo sapiens

Alignment Length:	1178	Identity:	276/1178 - (23%)
Similarity:	450/1178 - (38%)	Gaps:	370/1178 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly   436 TPNLRN-GSASAQISGSTSNSSNISLNSGSASAAGGGTPHSQQQQERSQAGKASTPPPIT----- 494
            ||:..| |..:....|.........|..|.|    |..|...||..|.:..|......:|
Human   674 TPSFANLGRTTLSTRGPPRGGPGGELPRGPA----GLGPRRSQQGPRKEPRKIIATVLMTEDIKL 734

  Fly   495 ----GRWIPPSLRPQHGLTQSE------KNDAVFRRVRGILNKLTPEKFQELSDELLKLDLNSIA 549
                ..|.|.|.|......:.|      |...:|||||.|||||||:.||:|..::.:|.:::..
Human   735 NKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEE 799

  Fly   550 ILKGVILLIFDKALDEPKYSSMYAQLCKRL-SEEAPSFDKEPSNSSTFLRLLIAVCRDKFNNRLK 613
            .|||||.|||:||:.||.:|..||.:|:.| :.:.|:.:| |:.:..|.:||:..|:.:|    :
Human   800 RLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEK-PTVTVNFRKLLLNRCQKEF----E 859

  Fly   614 RDENDNR--PPPENEADE--------------EERRHLAKQRMLGNVKFIGELNKLDMLSKNVLH 662
            :|::|:.  ...:.|.||              ||.|.:|::|.|||:||||||.||.||::.::|
Human   860 KDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMH 924

  Fly   663 QCIMELFDKKKKRTAGTQEMCEDMECLAQLLKTCGKNLDSEQGKELMNQYFEKLERRSKSSEYPP 727
            .|:::|.....:         |.:|||.:||.|.||:||.|:.|..|:|||.::|:..|..:...
Human   925 DCVVKLLKNHDE---------ESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSS 980

  Fly   728 RIRFMLKDVIELRQNNWVPRKLGTTEGPVPIKQIRSDDDSIIRTPFPNRNRDMRNNDRDSDSWMN 792
            ||||||:||::||.:|||||:  ..:||..|.||.                      ::::...:
Human   981 RIRFMLQDVLDLRGSNWVPRR--GDQGPKTIDQIH----------------------KEAEMEEH 1021

  Fly   793 RFHLNIQPGGYNDMFSGLSVTGASPIVSPFATSNRDRDRDNRQYNNRGDRNRDRDQGGSGGANYG 857
            |.|:.:|                                   |...:|.   |:.:||..|....
Human  1022 REHIKVQ-----------------------------------QLMAKGS---DKRRGGPPGPPIS 1048

  Fly   858 NRYNKHNQNGGGSGGGSSNNRDRDDSRRNNDRDRDRNDGQYGGNQLLGSKELAPRFKRNLITTNQ 922
            ......:..|..:...|..:|..|.||
Human  1049 RGLPLVDDGGWNTVPISKGSRPIDTSR-------------------------------------- 1075

  Fly   923 DAVENLQMRPAANSLLFRAASQNQKFPATLPMSTPPNSSGSNNQ------------GSSQGQLSN 975
                                         |...|.|.|..||||            |||.|..:.
Human  1076 -----------------------------LTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAK 1111

  Fly   976 SHYPMLGTPTSHLINPTRPSSTP-----------SAEYADNRTLL---GVQQERGLKATSSSPNF 1026
                    |:.......||:::.           ..|..|||.::   .:.:|||.||
Human  1112 --------PSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKA------- 1161

  Fly  1027 SSGAEKLDLDGQAGRKGSHDGKDQGKNGSVERPSTPAGGIGSAK--QQKKDKGPNKEELSKKASQ 1089
            ....::|:...:.|.:|..          ::|..|||.....:|  :::..:.|::.|..:||:.
Human  1162 GDRGDRLERSERGGDRGDR----------LDRARTPATKRSFSKEVEERSRERPSQPEGLRKAAS 1216

  Fly  1090 YFKDRFYYDEEEDAEVTEIEIKDIDETKTEEADAAPVEVSEVKEDAAIPVTTPDRFTKLVDGFLE 1154
            ..:||   |...||                           ||.:||:|..:|          |:
Human  1217 LTEDR---DRGRDA---------------------------VKREAALPPVSP----------LK 1241

  Fly  1155 LKLPEKALKDVCINLLMEVLDRVNDVFLERAVRLLQTLRKQSNIKPNIVVEIFKQVVNKMNEREA 1219
            ..|.|:.|:.....::.|.| .:||  ::.||:.:|.|     ..|:::....:..|....||.|
Human  1242 AALSEEELEKKSKAIIEEYL-HLND--MKEAVQCVQEL-----ASPSLLFIFVRHGVESTLERSA 1298

  Fly  1220 LNPRIVSLVASLLAKTICEPALLK------LSDIANFTDN------------------------- 1253
            :   ....:..||.:.:|...|..      |.:|....::
Human  1299 I---AREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGV 1360

  Fly  1254 ----------------GQHYPLFLLVLQQLVKVIGKDALEEKFRASKVDLMNSLPEADRNKERLA 1302
                            |:...|.|.:|..|.|.:|...:...:|.:.:.....|||.    :.:.
Human  1361 PMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEG----QDIG 1421

  Fly  1303 GILEDRKLSF------------LYPLLKVQAEMLKQLQSDPNPNNFYKWIKANVDNKYYKDPGFI 1355
            ..:.::|:.:            ..|..::..::.|.|:...:....:.||:||:..:.......:
Human  1422 AFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLV 1486

  Fly  1356 QALMTVV-VKYVTKETTLADNLDPKEHPEKSVTQKEQQLLENYSQMLQTFLGQNELQLMALYALQ 1419
            :||||.| ...:..||.|               :.:..:|:..:::||.:|...:.:|.||||||
Human  1487 RALMTAVCYSAIIFETPL---------------RVDVAVLKARAKLLQKYLCDEQKELQALYALQ 1536

  Fly  1420 TFCYNENFPKGMLCRWFKYLYESEIIEEEAFVLWKEEISDKYP----GKGSALFQVNAWLTWLQE 1480
            ........|..:|..:|..||:.::::|:||..|:   |.|.|    |||.||..|.|:..||:|
Human  1537 ALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWE---SSKDPAEQQGKGVALKSVTAFFKWLRE 1598

  Fly  1481 AESEDDED 1488
            ||.|.|.:
Human  1599 AEEESDHN 1606

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
NAT1	NP_001033943.2	MIF4G	518..743	CDD:280935	96/241 (40%)
		RAM	<817..865	CDD:291966	6/47 (13%)
		MA3	1128..1241	CDD:214714	26/112 (23%)
		W2_eIF4G1_like	1314..1460	CDD:211397	36/146 (25%)
EIF4G1	NP_001181875.2	PHA03247	<21..583	CDD:223021
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	173..229
		PABPC1-binding	179..207
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	241..326
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	339..607
		EIF4E-binding	614..625
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	674..720		13/49 (27%)
		eIF3/EIF4A-binding	689..1092		146/549 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	738..764		5/25 (20%)
		MIF4G	768..993	CDD:397130	94/238 (39%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1031..1234		55/327 (17%)
		MA3	1249..1361	CDD:397128	20/122 (16%)
		W2_eIF4G1_like	1445..1577	CDD:211397	37/149 (25%)
		EIF4A-binding	1457..1606		50/166 (30%)
		Necessary but not sufficient for MKNK1-binding	1592..1606		7/13 (54%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D594395at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR23253
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	3.020