DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment tutl and dscama

DIOPT Version :9

Sequence 1:	NP_001303307.1	Gene:	tutl / 46015	FlyBaseID:	FBgn0010473	Length:	1536	Species:	Drosophila melanogaster
Sequence 2:	XP_021334866.1	Gene:	dscama / 568643	ZFINID:	ZDB-GENE-050310-7	Length:	2025	Species:	Danio rerio

Alignment Length:	1664	Identity:	343/1664 - (20%)
Similarity:	556/1664 - (33%)	Gaps:	504/1664 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly   137 HITAILGEGVIFNCHV-EFPNDHPVPYVLQWDKKVSETGSDLPIYIWYESYPEHIEEGYKGRVSR 200
            ::|||.|..:..:||| .:|.     |.::|.|.
Zfish   513 NLTAIAGWDMYIHCHVIGYPY-----YSIKWFKN------------------------------- 541

  Fly   201 VSQDSPF--------GSASLNLTNI-RESDQGWYECKVVFLNRDPKQHKNGTWFHLDVHAPPRFS 256
             |...||        .:.:|.|.|: :|.|:|.|.|.|   ...|:..||.: .|:.|..||  .
Zfish   542 -SNLLPFNDRQRAFENNGTLKLLNVQKELDEGEYSCHV---QVQPQLFKNQS-VHVTVKVPP--F 599

  Fly   257 VTPEDIIYVNLGDSIILNCQADGTPTP-EILWYKDANPVDPSPTVGIFN-DGT-ELRISTIRHED 318
            :.|.:....::|..:.:.|.......| .|.|.||...::.|..|.|.| |.| .||||.::...
Zfish   600 IQPFEFPRYSIGHRVFVPCVVRSGDLPISITWEKDGKSINASLGVTIDNIDFTSSLRISNLQRVH 664

  Fly   319 IGEYTCIARNGEGQVSHTARVIIAGGAVIMVPPTNQTKLEGEKVIFSCEAKAMPGNVTVRW-YRE 382
            .|.|||||:|....|.:.:::|:.......|.|.:|..:.|:.||.:|.|...| ..|:.| |.:
Zfish   665 NGTYTCIAQNDAAVVKYQSQLIVRVPPRFKVQPQDQDGIYGKSVILNCSADGEP-RPTIEWKYSK 728

  Fly   383 GS--PVREVAALET--RVTIRKDGSLIINPIKPDDSGQYLCEVTNGIGDPQSASAYLSVEYPAKV 443
            |:  |..:..||.:  ||.:..:|||:|..:..:|:|.|||:|:|.:|...|.|.||:|:.||.:
Zfish   729 GAGVPQFQPIALNSGFRVQLLGNGSLLIKHVLEEDAGYYLCKVSNDVGADVSKSMYLNVKIPAMI 793

  Fly   444 TFTPTVQYLPFRLAGVVQCYIKSSPQLQYVTWTKD------KRLLEPYQMKDIVVMAN------G 496
            |..|............:.|.......:. |.|.|:      ..::.|...:..|.:.|      .
Zfish   794 TSYPNNSLATKGEKIEMSCKAHGEKPIM-VRWEKEVEKEKQSHMINPDMWRHTVTVKNVGDEVVS 857

  Fly   497 SLLFTRVNEEHQGQYACTPYNAQGTAGASGVMDVLVRKPPAFTVEPETLYQRKV----------- 550
            :|.......|..|.::|...|:.|.  ..|::.:.|::||    ||..:..|:|
Zfish   858 TLQIYPTMREDSGFFSCHAINSYGE--DRGILQLTVQEPP----EPPKVEIREVKERTIALRWTM 916

  Fly   551 ---GDSV----EMHCDALEAEGTERPTIKWQRQEGEQLTESQRNR-IKISGGNITIENLRREDFG 607
               |:|:    ::.|        :..|..|:|        ::|.| :..:....||..|......
Zfish   917 GFDGNSLITGYDIEC--------KNKTETWER--------ARRTRDVSPTLNQATIIELHPSSTY 965

  Fly   608 YYQCVVSNEVATLMAVTQLVI---EGTQPHAPYNITGKA-TESSITLQW---LPGYSGGSEYKQD 665
            ..:....|.:.......:|.:   |......|.::|.:| |..||.:.|   |.....|  ..:.
Zfish   966 NIRMFAKNHIGDSEPSNELTVTTDEADPEGPPQDVTLEAITSQSIKVTWKAPLKHLQNG--VIRG 1028

  Fly   666 YTIWFREAGVN---DWQTISVTPSGSTQ-VTINGLASGTTYEFQVVGRNVLGDGMMSKVMTVRTL 726
            |.:..||...|   .:..|||..:|.|: :::|.|.....||..|...|..|.|..|..:...|:
Zfish  1029 YQVCHREHSTNGSHQFVCISVEATGETESLSLNNLKKFAEYEVVVQASNSAGPGPASSEVRATTM 1093

  Fly   727 EDAPA-APRNVKAATQPPDSF---FQLMPDEA--DLLAYFDIYFHT--------DSRGTLVYSPP 777
            ||.|: ||..|.|....|:|.   :|....||  .:|..|.|.:..        :.|....:..|
Zfish  1094 EDVPSRAPEKVVATAASPESISLSWQTPAREALNGVLQGFRIIYWANLPDGELGEIRNVTTHKAP 1158

  Fly   778 -------------------------------KLRVKGPKPGPPRNVSVTEVSNGFL-ITWQSPLE 810
                                           .:|.|...||||..|.....|:..: ::|..||:
Zfish  1159 LELEGLEKYTNYSIQVLAFTRAGDGVRSDQIYIRTKEDVPGPPGGVKAAAASSSVVYVSWLPPLK 1223

  Fly   811 RAHIVKFYTIKYRTDAQWKTLNRGQIRPEETQYLVKNLVGGRTYYFRVLA-NSEKSYESSDEVKF 874
            ...:::.||: :.:......::..::.|:|..:.|.||...|.|...|:| .:.....|||.:..
Zfish  1224 LNGVIRKYTV-FCSSTSPTVVSEFEVAPDEFLHRVHNLSRNRKYNIWVMAVTAAGRGNSSDVITV 1287

  Fly   875 PVPARVKHKAIT-AGVVGGILFFIVAIILSVCAVKICNKRKRRKQEKEFNMVACRITDARN---- 934
            ...|:...|.:| :|.|  ...::..::|...||.  :.....|..||....|..:.|.|.
Zfish  1288 EPLAKAPAKILTFSGTV--TTPWMRDVVLPCRAVG--DPPPAIKWLKESGSPAPAVIDGRRSIHG 1348

  Fly   935 -------------------IAANNHHLHNRSTGSISSG---QVPLKKYKQTRISSLTAILIAILH 977
                               :|:||:     .|..|...   |||..:.:.| ::..|...|.:
Zfish  1349 NGSFIIKTVKAEDSGYYTCVASNNY-----GTDEIILNLQVQVPPDQPRLT-VTKTTTTSITV-- 1405

  Fly   978 WIWPPDRCTNCHSIYSSPNLEDGDEDDGGGGRRRSVSRIQRSLDGRFVLDVEGVSKLGYSQQTLE 1042
             .|.|.                   |:||...|..:                    |.||:...|
Zfish  1406 -TWTPG-------------------DNGGSSIRGYI--------------------LQYSEDNSE 1430

  Fly  1043 S-GNVDVVDGGLFERRNSNVSQKSSSDDGGFLSRRNFITARASWRRPLVASSSQLSLQSAADSAR 1106
            . |::.:                |.|:....|...|.    .:|.:..:.:
Zfish  1431 KWGSISI----------------SPSERSYRLENLNC----GTWYKFTLTA-------------- 1461

  Fly  1107 GFLQGLLKIGAKQSAVPPNSQSYFDEAVSGAR---YQNVLRPYTSSN------NLYGNADRSRPL 1162
                        |:||.|...|...||.:..:   :......:||.|      ||.|..|...|:
Zfish  1462 ------------QNAVGPGRISEIIEAKTHGKEPQFSKEQELFTSINGTSVKLNLIGWNDGGCPI 1514

  Fly  1163 HINTISGSLSQQQQLYTP-----SRISRIFSSSPQQLQP----HHQQLLLSSGGSGAYPTHFSDL 1218
            ...|:     :.:.|.:|     .|.|...|.:...||.    ..|..:.:|.|.......|:.|
Zfish  1515 TSFTL-----EYRPLESPVWTKAKRTSLSKSYNLLDLQEATWYELQMKVYNSAGLAEKRVKFATL 1574

  Fly  1219 S---TVYPP-------NSAERSSHN----------------------LSSRYRYYSQELPSLR-- 1249
            |   :..||       :..:::|:.                      :..|.|...|.|..||
Zfish  1575 SYDGSTIPPLVKTLVKDPVKKTSNEGMKMMVTISCILVGMVLLFVLLMVLRRRRREQRLKRLRDA 1639

  Fly  1250 -----------TIQEETRRQQQQ--KQH-----------------PLEDHFVPLQLPSPPSWRSY 1284
                       |...:|..:|||  :.|                 .::|....|...:.
Zfish  1640 KSLAEMLMSKNTRPSDTMNKQQQTLRMHIDIPRAQLLIEERDTMETVDDRSTVLLTDND------ 1698

  Fly  1285 YQSQASYRPRTRWYPRHHSRLFSNRQQQHEMLSPLPQLNLNLRNSMNPGGLEAS---PESRSSSS 1346
            :...|..:..|..:..|:       |...:...||..:     :.:.||..::|   |.||.::.
Zfish  1699 FGETAKQKSATVTHSVHY-------QSLSQATGPLVDV-----SDVRPGTSKSSLSNPTSRRTAK 1751

  Fly  1347 G-------FGSKNTSNHPGS-------TSEWRLLPPYRAPPSPPKHSGYFGGQQAQG-------- 1389
            .       :.|:.|.|.|..       :::|||..| |...|..|.|..:....:|.
Zfish  1752 AGPAARNRYASQWTLNRPHPPVSVHTLSTDWRLGTP-RVAGSVDKESDSYSVSPSQDTDRARSSM 1815

  Fly  1390 -QTPHGSYSYPRATT-------------PPYTMAHWL-------EMISRLNAATDSNLPKAPCPV 1433
             .|...|.:|.....             ..:|:....       :|.:..|..|||.....|
Zfish  1816 VSTESASSTYEELARAYEHAKMEEQLRHAKFTITECFISDTSSEQMTAGTNDYTDSLTSSTP--- 1877

  Fly  1434 DVGSVDGHYEFDPATPTPSASSMLREDLNLHIDTHPYHHHTLGPLSGSLLHSHAPYGGGARKQRS 1498
               |..|...|..:.|.|.....:   :|:.:   |..|...|.|
Zfish  1878 ---SESGICRFTASPPKPQDVCRV---INMAV---PKAHRPGGEL-------------------- 1913

  Fly  1499 LPAHLPRYDNVEVRLQAMREEFYAYR------KRQAMQQ 1531
              .|||.|         :|.:|...|      :..||.|
Zfish  1914 --VHLPPY---------LRMDFLLNRGVSGTSREAAMGQ 1941

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
tutl	NP_001303307.1	V-set	137..250	CDD:284989	27/122 (22%)
		IG_like	137..229	CDD:214653	22/101 (22%)
		I-set	253..341	CDD:254352	27/90 (30%)
		IGc2	268..331	CDD:197706	24/65 (37%)
		I-set	346..437	CDD:254352	34/95 (36%)
		Ig	349..437	CDD:299845	34/92 (37%)
		Ig	459..530	CDD:299845	14/82 (17%)
		IG_like	549..628	CDD:214653	14/97 (14%)
		IGc2	551..617	CDD:197706	12/70 (17%)
		FN3	633..725	CDD:238020	27/99 (27%)
		FN3	786..874	CDD:238020	23/89 (26%)
dscama	XP_021334866.1	Ig	124..217	CDD:325142
		I-set	236..311	CDD:254352
		IG_like	321..402	CDD:214653
		I-set	408..502	CDD:333254
		IGc2	524..579	CDD:197706	19/94 (20%)
		Ig	614..679	CDD:319273	23/64 (36%)
		Ig_DSCAM	708..787	CDD:143211	30/79 (38%)
		Ig	805..898	CDD:325142	18/99 (18%)
		FN3	894..988	CDD:238020	19/113 (17%)
		FN3	995..1092	CDD:238020	27/98 (28%)
		fn3	1100..1186	CDD:306538	14/85 (16%)
		fn3	1199..1282	CDD:306538	19/83 (23%)
		Ig	1312..1377	CDD:319273	13/71 (18%)
		fn3	1405..1471	CDD:306538	20/153 (13%)
		FN3	1492..1563	CDD:238020	18/75 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3510
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10075
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.910