DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment tutl and robo4

DIOPT Version :9

Sequence 1:	NP_001303307.1	Gene:	tutl / 46015	FlyBaseID:	FBgn0010473	Length:	1536	Species:	Drosophila melanogaster
Sequence 2:	XP_689255.3	Gene:	robo4 / 560765	ZFINID:	ZDB-GENE-020809-1	Length:	1134	Species:	Danio rerio

Alignment Length:	1245	Identity:	255/1245 - (20%)
Similarity:	405/1245 - (32%)	Gaps:	399/1245 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   233 NRDPKQHKNGTWFHLDVHAPPRFSVTPEDIIYVNLGDSIILNCQADGTPTPEILWYKDANPVD-- 295
            :||....:.|:....:::. ||....|.|:: |.:|....|:|:|:|.|.|.|.|.::..|:|
Zfish    52 HRDRAHRRKGSRLVSEINL-PRIVHHPSDVV-VRVGSPATLSCRAEGNPEPTIQWLRNGQPLDTD 114

  Fly   296 -----PSPTVGIFNDGTELRISTI------RHEDIGEYTCIARNGEGQ-VSHTARVIIAG-GAVI 347
                 ..|.|  ..||:....|.:      .||.:  |.|||.|..|. .|..|.:.||. ....
Zfish   115 KMDAQSQPIV--LPDGSLFFFSVVPGRKGQSHEAV--YACIAHNSIGNATSRNASLHIAALREDF 175

  Fly   348 MVPPTNQTKLEGEKVIFSCEAKAMPGNVTVRWYREGSPVREVAALETRVTIRKDGSLIINPIKPD 412
            .|.|::.....||....:|..........|.|.::|..:.......|.:    .|.|||.|.:.:
Zfish   176 RVQPSDVEVAIGEMATINCSPPVGHPEPNVTWRKDGILINSSNEHYTEL----KGKLIIAPAQKN 236

  Fly   413 DSGQYLCEVTNGIGDPQSASAYLSVEYPAKVTFTPTVQYLPFRLAGVVQCYIKSSPQLQYVTWTK 477
            |||.|.|..:|.||..:|.:|.|||
Zfish   237 DSGVYSCIASNMIGVRESRAARLSV---------------------------------------- 261

  Fly   478 DKRLLEPYQMKDIVVMANGSLLFTRVNEEHQGQYACTPYNAQGTAGASGVMDVLVRKPPAFTVEP 542
               |.:|                                             ||:|||...:|:
Zfish   262 ---LAKP---------------------------------------------VLLRKPEDVSVQ- 277

  Fly   543 ETLYQRKVGDSVEMHCDALEAEGTERPTIKWQRQEGEQLTESQRNRIKIS-GGNITIENLRREDF 606
                   :|:|.:..|   ||:|...|:|:|.|::|    .....|..|: ..::.|..:..:|.
Zfish   278 -------LGESAQFFC---EADGDPMPSIEWSREQG----PLPNGRYLINPDHSLQIHYVTAQDM 328

  Fly   607 GYYQCVVSNEVATLMAVTQLVIE---GTQPHAPY-----------NITGKATESSIT-LQW-LPG 655
            |.|.|.|.|::...:|..||::|   ||:....:           |:|..:|.|::: :.| |..
Zfish   329 GRYSCTVENKLGVSVASAQLLVEDAGGTRLRDLHKELSALRVSLENVTVMSTASNMSQVMWKLQS 393

  Fly   656 YSGGSEYKQDYTIWFRE--AGVNDWQTISVTPSGSTQVTINGLASGTTYEFQV--VGRNVLGDGM 716
            :.....|.:.:.:.:|.  ...:|| |....|..|....:..|..|..|||:|  .|.::.  |.
Zfish   394 FGSQPHYLEGFEVLYRSLLPASSDW-TAQRVPQPSLHTHVGPLKRGYKYEFKVRPYGSSLY--GR 455

  Fly   717 MSKVMTVRTLEDAP-AAPRNVKAATQPPDSFFQLMPDEADLLAYFDIYFHTDSRGTLVYSPPKLR 780
            .|....:|..|..| |||::|...          ||.|
Zfish   456 ESNTRHLRVPEIVPSAAPQDVSIT----------MPAE--------------------------- 483

  Fly   781 VKGPKPGPPRNVSVTEVSNGFLITWQSPLERAH--IVKFYTI-----KYRTDAQWKTLNRGQIRP 838
                     ||.:|.       ::|:.|...||  |::.|.:     :.:|...| |::.|....
Zfish   484 ---------RNDTVH-------LSWEPPPHDAHNGIIQGYQVWCVESEEQTSFNW-TVDSGTHSI 531

  Fly   839 EETQYLVKNLVGGRTYYFRVLA-NSEKSYESSDEVKFPVPARVKHKAITAGVVG----------- 891
            |     :..|:..|.|:..|.| |.......||..|..:.:|::......|::.
Zfish   532 E-----IFTLLPSRLYWVTVAAVNGAGVGVQSDPYKLLIESRLEATPYQTGILSMSHFVSVIKEP 591

  Fly   892 ------GILFFIVAIILSVCAVKICNKRKRRKQEKEFNMVACRITDARNIAANNHHLHNRSTGSI 950
                  |.|.:.|.:|.:|...:                               .|:...:....
Zfish   592 VFIGSVGTLLWCVLMITAVFLYR-------------------------------RHIRPANPSGK 625

  Fly   951 SSGQVPLK------KYKQTRISSLTAILIAILHWI---WPPDRCTNCHSIYSSPNLEDGDEDDGG 1006
            |||...|:      |::.....|         .||   |.|...:.......:|:.|:
Zfish   626 SSGLYRLESEDLIIKHRMAAPDS---------PWISGGWRPGSNSEPKQGLWTPSQEN------- 674

  Fly  1007 GGRRRSVSRIQRSLDGR-------FVLDVEGVSKLGYSQQTLESGNVDVVDGGLFERRNSNVSQK 1064
            .|.||:...|....|..       .|.|..||....|         ||:...||   :..|...:
Zfish   675 SGLRRTTLPITVKKDPNPLRSAVPIVPDNCGVYGTFY---------VDLTGNGL---KTFNSPGR 727

  Fly  1065 SSSDDGGFLSRRNFITARAS----------------WRRPLVASSSQ-LSLQSAADSARGFLQG- 1111
            ......|....:|..|.|.:                |:|.|.|..:. :..:|...:.:..|..
Zfish   728 CPRMPHGNSQLQNSETIRITEPIVKTAVVRELQALPWKRTLPAQPNMGVKKESWEKNVKRELHAV 792

  Fly  1112 ----LLKIGAKQSAV--PPNSQSYFDEAVSGARYQNVLRP--------YTSSNNLYGNADRSRPL 1162
                |:.:..:..||  .||.|..|.:..:|...:.| :|        |::|..|........||
Zfish   793 KSAPLMPLNQQALAVCSAPNHQQRFSQHPAGGVSEPV-KPLSSPRILHYSASLQLVDMLPSPPPL 856

  Fly  1163 HINTISGSLSQQQQLYTPSRISRIFSSSPQQLQPHHQQLLLSSGGSGAYPTHFSDLSTVYPPNSA 1227
            .:.. :.|||.:.:....::::....|        .|.:..:||..|.     :..:|..|.|
Zfish   857 PMED-NHSLSSEDESTRSTKLTVDIGS--------QQSVCAASGLQGP-----ASANTGCPNN-- 905

  Fly  1228 ERSSHNLSSRYRYYSQELPSLRT--IQEETRRQQQQKQHPLEDHFVPLQL-PSPPSWRSYYQSQA 1289
             .|...||..|.:.|.....|.|  .|:.|...|...|:        ::: |.|....:.:||..
Zfish   906 -HSPAQLSPSYSHLSTASFCLSTDDYQDTTLSTQGHTQY--------MEISPKPQGQSTSHQSTP 961

  Fly  1290 SYRPRTRWYPRHHSRLFSNRQQQHEMLSPLPQLNLNLRNSMNPGGLEASPESRSSSSGFGSKNTS 1354
            | .||.                    .||.|.|..    ...||..|...|..:...|.......
Zfish   962 S-MPRP--------------------FSPTPTLGY----ICGPGDREMDEEQETVPIGLRRATLR 1001

  Fly  1355 NHPGS-TSEW 1363
            :.|.| .|||
Zfish  1002 STPSSCCSEW 1011

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
tutl	NP_001303307.1	V-set	137..250	CDD:284989	3/16 (19%)
		IG_like	137..229	CDD:214653
		I-set	253..341	CDD:254352	31/101 (31%)
		IGc2	268..331	CDD:197706	23/75 (31%)
		I-set	346..437	CDD:254352	25/90 (28%)
		Ig	349..437	CDD:299845	25/87 (29%)
		Ig	459..530	CDD:299845	2/70 (3%)
		IG_like	549..628	CDD:214653	23/79 (29%)
		IGc2	551..617	CDD:197706	20/66 (30%)
		FN3	633..725	CDD:238020	22/108 (20%)
		FN3	786..874	CDD:238020	22/95 (23%)
robo4	XP_689255.3	Ig1_Robo	70..169	CDD:143317	31/104 (30%)
		I-set	71..168	CDD:254352	31/101 (31%)
		I-set	175..261	CDD:254352	25/89 (28%)
		Ig2_Robo	177..261	CDD:143201	25/87 (29%)
		I-set	265..350	CDD:254352	30/144 (21%)
		Ig	282..350	CDD:299845	21/74 (28%)
		FN3	373..448	CDD:214495	19/75 (25%)
		FN3	472..560	CDD:238020	27/146 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3510
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10075
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.910