DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment shi and Dnm1l

DIOPT Version :9

Sequence 1:	NP_001259588.1	Gene:	shi / 45928	FlyBaseID:	FBgn0003392	Length:	896	Species:	Drosophila melanogaster
Sequence 2:	NP_001346936.1	Gene:	Dnm1l / 74006	MGIID:	1921256	Length:	755	Species:	Mus musculus

Alignment Length:	825	Identity:	301/825 - (36%)
Similarity:	439/825 - (53%)	Gaps:	146/825 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MDSLITIVNKLQDAFTSLGVHMQLDLPQIAVVGGQSAGKSSVLENFVGKDFLPRGSGIVTRRPLI 65
            |::||.::|||||.|.::|..: :.||||.|||.||:|||||||:.||:|.||||:|:|||||||
Mouse     1 MEALIPVINKLQDVFNTVGADI-IQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGVVTRRPLI 64

  Fly    66 LQLI--------------NG---------------------VTEYGEFLHIKGKKFSSFDEIRKE 95
            |||:              ||                     ..|:|:|||.|.|.::.|||||:|
Mouse    65 LQLVHVSPEDKRKTTGEENGKFQSWNPATWKNSRHLSKGVEAEEWGKFLHTKNKLYTDFDEIRQE 129

  Fly    96 IEDETDRVTGSNKGISNIPINLRVYSPHVLNLTLIDLPGLTKVAIGDQPVDIEQQIKQMIFQFIR 160
            ||:||:|::|:|||:|..||:|:|:||:|:||||:||||:|||.:||||.|||.||:::|.:||.
Mouse   130 IENETERISGNNKGVSPEPIHLKVFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFIS 194

  Fly   161 KETCLILAVTPANTDLANSDALKLAKEVDPQGVRTIGVITKLDLMDEGTDARDILENKLLPLRRG 225
            ....:|||||.||||:|.|:|||:::||||.|.||:.|||||||||.||||.|:|..:::|::.|
Mouse   195 NPNSIILAVTAANTDMATSEALKISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLG 259

  Fly   226 YIGVVNRSQKDIEGRKDIHQALAAERKFFLSHPSYRHMADRLGTPYLQRVLNQQLTNHIRDTLPG 290
            .||||||||.||..:|.:..::..|..|.  ...|..:|:|.||.||.|.||:.|.:||||.||.
Mouse   260 IIGVVNRSQLDINNKKSVTDSIRDEYAFL--QKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPE 322

  Fly   291 LRDKLQKQMLTLEKEVEEFKHFQPGDASIKTKAMLQMIQQLQSDFERTIEGSGSALVNTNELSGG 355
            |:.::  .:|..:.:.....:.:|.|.  |:..:||:|.:..:::..||||: :..:.|:||.||
Mouse   323 LKTRI--NVLAAQYQSLLNSYGEPVDD--KSATLLQLITKFATEYCNTIEGT-AKYIETSELCGG 382

  Fly   356 AKINRIFHERL--RFEIVKMACDEKELRREISFAIRNIHGIRVGLFTPDMAFEAIVKRQIALLKE 418
            |:|..||||..  ..|.|........:  :|..||||..|.|..||.|:::||.:|||||..|:|
Mouse   383 ARICYIFHETFGRTLESVDPLGGLNTI--DILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEE 445

  Fly   419 PVIKCVDLVVQELSVVVRMC----TAKMSRYPRLREETERIITTHVRQREHSCKEQILLLIDFEL 479
            |.::||:||.:|:..:::.|    |.::.|:|:|.:....::|..:|:|.....|.:..|:..||
Mouse   446 PSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIEL 510

  Fly   480 AYMNTNHEDF---IGFANAQNKSENANKTGTRQLGNQVIRKGHMVIQNLGIMKDIKLGRSGPLNV 541
            ||:||.|.||   .|..|...:.:..|:. .|:|
Mouse   511 AYINTKHPDFADACGLMNNNIEEQRRNRL-AREL------------------------------- 543

  Fly   542 RFFSPSTGGSRPYWFVLTSESISWYKDEDEKEKKFMLP------------LDGLKLRDIEQGFMS 594
                ||.|.                :|:..|....:.|            .||..::|
Mouse   544 ----PSAGS----------------RDKSSKVPSALAPASQEPPPAASAEADGKLIQD------- 581

  Fly   595 MSRRVTFALFSPDG--------------RNVYKDYKQLELSCETVEDVESWKASFLRAGVYPEKQ 645
             :||.|..:.|..|              |.:.|..|..||..|.    :|.....:.|.  |:|.
Mouse   582 -NRRETKNVPSAGGGIGDGGQEPTTGNWRGMLKTSKAEELLAEE----KSKPIPIMPAS--PQKG 639

  Fly   646 ETQENGDESASEESSSDPQLERQVETIRNLVDSYMKIVTKTTRDMVPKAIMMLIINNAKDFINGE 710
            ......|...........:.:|..|.|..|:.||..||.|..:|.||||:|..::|:.||.:..|
Mouse   640 HAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSE 704

  Fly   711 LLAHLYASGDQAQMMEESAESATRREEMLRMYRACKDALQIIGDV 755
            |:..||.|.....::.||.:.|.||:|...|.:|.:.|.|||.::
Mouse   705 LVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEI 749

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
shi	NP_001259588.1	DYNc	1..240	CDD:197491	147/273 (54%)
		CrfC	73..>498	CDD:223771	192/433 (44%)
		Dynamin_M	212..498	CDD:279383	105/294 (36%)
		PH_dynamin	512..644	CDD:269958	23/157 (15%)
		PH	552..638	CDD:278594	18/111 (16%)
		GED	664..755	CDD:128597	35/90 (39%)
		DUF4527	<758..819	CDD:291689
		Drf_FH1	<761..>846	CDD:283903
Dnm1l	NP_001346936.1	DLP_1	23..321	CDD:206738	155/299 (52%)
		G1 motif. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01055	32..39		4/6 (67%)
		G2 motif. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01055	58..60		1/1 (100%)
		G3 motif. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01055	165..168		2/2 (100%)
		G4 motif. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01055	234..237		2/2 (100%)
		Dynamin_M	245..528	CDD:307257	105/291 (36%)
		G5 motif. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01055	264..267		2/2 (100%)
		Middle domain. /evidence=ECO:0000250\|UniProtKB:O00429	363..508		52/147 (35%)
		Interaction with GSK3B. /evidence=ECO:0000250\|UniProtKB:O00429	467..704		66/302 (22%)
		B domain. /evidence=ECO:0000250\|UniProtKB:O00429	521..588		17/126 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	541..573		8/82 (10%)
		C-terminal dimerization domain. /evidence=ECO:0000250	561..755		55/203 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	585..607		3/21 (14%)
		GED	661..749	CDD:308047	35/87 (40%)
		Important for homodimerization. /evidence=ECO:0000250\|UniProtKB:O00429	673..687		6/13 (46%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0699
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D141880at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100232
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.720