Protein Alignment Pex1 and Spast

DIOPT Version :10

• Sequence 1: NP_652016.1
• Gene: Pex1 / 45460
• FlyBaseID: FBgn0013563
• Length: 1006
• Species: Drosophila melanogaster
• Sequence 2: NP_001156342.1
• Gene: Spast / 50850
• MGIID: 1858896
• Length: 614
• Species: Mus musculus
• Alignment Length: 276
• Identity: 83/276 - (30%)
• Similarity: 139/276 - (50%)
• Gaps: 17/276 - (6%)

  Fly   717 MRVEELPGLESVVGVLEEVLMWPSRYPTIFNA--SPLRNQAGVLLYGPPGTGKTYLVSQLATSWN 779
            ::.:::.|.|.....|:|:::.||..|.:|..  :|.|   |:||:||||.|||.|...:|...|
Mouse   337 VKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPAR---GLLLFGPPGNGKTMLAKAVAAESN 398

  Fly   780 LRIISVKGPELLAKYIGQSEENVRNLFNRARSARPCVLFFDEFDSLAPKRGHDSTGVTDRVVNQL 844
            ....::....|.:||:|:.|:.||.||..||..:|.::|.||.|||..:|.......:.|:..:.
Mouse   399 ATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEF 463

  Fly   845 LTELDGVE--GLQGVTVIAATSRPELLDPALLRSGRIDRLVECPLPDAPARVRIFEAL----SST 903
            |.|.|||:  |...|.|:.||:||:.||.|:||  |..:.|...||:...|:.:.:.|    .|.
Mouse   464 LIEFDGVQSAGDDRVLVMGATNRPQELDEAVLR--RFIKRVYVSLPNEETRLLLLKNLLCKQGSP 526

  Fly   904 LSLDECVDFDWFAGKTANYTGADIQSILTSANMAAVKEAL-AQFGHEKLAKKISLKQKHLIESFQ 967
            |:..|...   .|..|..|:|:|:.::...|.:..::|.. .|..:...::..:::.....||.:
Mouse   527 LTQKELAQ---LARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLK 588

  Fly   968 TTRPSLSASDVAKYHR 983
            ..:.|:|...:..|.|
Mouse   589 KIKRSVSPQTLEAYIR 604

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pex1	NP_652016.1	PEX-1N	93..168	CDD:462729
		CDC48	365..983	CDD:273521	82/274 (30%)
		RecA-like_PEX1_r2	728..885	CDD:410934	60/160 (38%)
Spast	NP_001156342.1	Required for interaction with RTN1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..298
		Required for midbody localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..192
		Required for interaction with ATL1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..78
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..48
		Required for nuclear localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..48
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	48..85
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	57..65
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	91..112
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000250|UniProtKB:Q9UBP0	110..194
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	112..198
		MIT_spastin	114..193	CDD:239142
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	220..306
		Sufficient for microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	226..614		83/276 (30%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	268..326
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	307..310
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	308..310
		RecA-like_spastin	341..504	CDD:410932	62/167 (37%)
		AAA_lid_3	531..585	CDD:465537	9/56 (16%)
		Vps4_C	<578..610	CDD:462762	6/27 (22%)