Protein Alignment fwd and PIK3C2A

DIOPT Version :9

• Sequence 1: NP_001356971.1
• Gene: fwd / 45374
• FlyBaseID: FBgn0004373
• Length: 1682
• Species: Drosophila melanogaster
• Sequence 2: NP_001308307.1
• Gene: PIK3C2A / 5286
• HGNCID: 8971
• Length: 1686
• Species: Homo sapiens
• Alignment Length: 1565
• Identity: 298/1565 - (19%)
• Similarity: 549/1565 - (35%)
• Gaps: 460/1565 - (29%)

  Fly   238 NGNGSTVPAFLMAAPVTLPIGGQMP--TIKCLPGASSAASTPKHSRYSKP------RLSLSRVF- 293
            ||....:|.|....|:.|.:.||.|  :....|      :||.|.:.|.|      ...::::|
Human   170 NGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTP------ATPFHPQGSLPIYRPVVSTDMAKLFD 228

  Fly   294 -----NHSMPSVHGRPNLSLAPAGVPGSVNPPKRISTHQRNLSLDFRSMGIL-LPPVSQVTNNHI 352
                 :..:.:...|.:|.:..:.|......||         |.|......| |.|:|:...:::
Human   229 KIASTSEFLKNGKARTDLEITDSKVSNLQVSPK---------SEDISKFDWLDLDPLSKPKVDNV 284

  Fly   353 NHTQHHRNRSLDSALQRIPEVEVSSPNAESENTFCSPSILGATMCSKIATTAATVTSALSPPGTP 417
            ....|...:::.|.|.:.|...|......:.|......:.|.::      :.||||.:.|
Human   285 EVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSL------SVATVTRSQS----- 338

  Fly   418 TPALMSTACTPTIGAVKGNVQHPVPKHEQSI---------------SLAAACRSSAVVESCPTLR 467
                 ....|..:...:|::....|....|:               .:||.|||        ..:
Human   339 -----LNIRTTQLAKAQGHISQKDPNGTSSLPTGSSLLQEVEVQNEEMAAFCRS--------ITK 390

  Fly   468 MRPKSSESVAANGQEMIGVIVSSTGNGSSSSNNSCSSAGSTKKREDL------TSLGSDDSGIIC 526
            ::.|...:   |.:...|.::|..    ::..|.|....|.|...|:      .:...|.|..:
Human   391 LKTKFPYT---NHRTNPGYLLSPV----TAQRNICGENASVKVSIDIEGFQLPVTFTCDVSSTV- 447

  Fly   527 GSESDQISLNRICHSHESLDSGEMDADADAEEECVDLMDTTSIDEEYNIMQPDHLCLYPPQAAST 591
                :.|.:..:|..|:.|:  ::|..:...:.|          .:..::|.:| ||     .|.
Human   448 ----EIIIMQALCWVHDDLN--QVDVGSYVLKVC----------GQEEVLQNNH-CL-----GSH 490

  Fly   592 ELDCRTLRPSRKQKRQQQQVLERKNLPSQNQIESEDSVDAGVDARTR-YRVMN-----MSQAAIN 650
            |    .::..||...:.:..|...:...||...:.:..:..||.... |::..     |::..:.
Human   491 E----HIQNCRKWDTEIRLQLLTFSAMCQNLARTAEDDETPVDLNKHLYQIEKPCKEAMTRHPVE 551

  Fly   651 VELGTASNEIDPDVNYRESHQ--DPIADA--PSLSTPSVASTASATPSVTPTPIATPTPTESTKN 711
            ..|.:..|:::..:.....|:  |.:..|  ...|......|.:.|.||.....|...|...|.:
Human   552 ELLDSYHNQVELALQIENQHRAVDQVIKAVRKICSALDGVETLAITESVKKLKRAVNLPRSKTAD 616

  Fly   712 DQVLFKNFFGATKNAIFRTAQSIIENHEKKNAAKQKEQPDHSGTVSLVGSPNGGSSVPQDLV--- 773
                ..:.||....:...|..|:              .|::...||:    |..::...||:
Human   617 ----VTSLFGGEDTSRSSTRGSL--------------NPENPVQVSI----NQLTAAIYDLLRLH 659

  Fly   774 ----KSPTDISKKKEFFSLLTSSSSIKAAAAAAANSAASTPPATPTEALAANSNSKLQPLSDPSA 834
                :||||.::         ||.|:|.|             .|.||.|.
Human   660 ANSGRSPTDCAQ---------SSKSVKEA-------------WTTTEQLQ--------------- 687

  Fly   835 DLKVKERTLNLRLPGDSEGSEGALTKCSSINSIHKLKLPVPGVVKYFISEKAPVSDVLQKPEKGQ 899
                                       .:|.:.|       |:...::|
Human   688 ---------------------------FTIFAAH-------GISSNWVS---------------- 702

  Fly   900 SGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFS-FPDQEVDLY--------IPQLVVMY 955
                          ::..:||..|     ||..|..:|. ...::|..|        ..:|::..
Human   703 --------------NYEKYYLICS-----LSHNGKDLFKPIQSKKVGTYKNFFYLIKWDELIIFP 748

  Fly   956 IQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNS--HNSSRKSKLALMKEIFSK 1018
            ||:.:|                  .|:.:..|..:.....|.|:|  .|..||...||.|.....
Human   749 IQISQL------------------PLESVLHLTLFGILNQSSGSSPDSNKQRKGPEALGKVSLPL 795

  Fly  1019 REHKQTQNDLKSAGTGGLHV---GEPRSVVALAKKTHHRSQSDATVLLADFRSPHTLSISHRMYQ 1080
            .:.|:    ..:.||..|::   ....||.....|..:  ..:..||..||.||....|
Human   796 FDFKR----FLTCGTKLLYLWTSSHTNSVPGTVTKKGY--VMERIVLQVDFPSPAFDII------ 848

  Fly  1081 QPPYTTQTLPTTPAKLCLGDLTSGHAFDNGCTCFETVRGQVNGLLGQRTLCSCGAPKTSPQKEFM 1145
               |||..:..:        :...|..:   |....::|::..:|.:.:  |.|..|  ..|.|:
Human   849 ---YTTPQVDRS--------IIQQHNLE---TLENDIKGKLLDILHKDS--SLGLSK--EDKAFL 895

  Fly  1146 -----------KALMNVGKNLTSWPSKAEKTSALRMSLNLINKNLPARVWLPLYSDIPHHVVRIT 1199
                       ..|..:..:..:|     |...|..:.:|:::      |..||   |...:.:.
Human   896 WEKRYYCFKHPNCLPKILASAPNW-----KWVNLAKTYSLLHQ------WPALY---PLIALELL 946

  Fly  1200 EEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPKMMPSLRHTKSEEHLDGSCLN---------- 1254
            :.|.|  :.:.::..:.::|.  |.|...:.|:|:.:.:|::   |.:|:.|.:.
Human   947 DSKFA--DQEVRSLAVTWIEA--ISDDELTDLLPQFVQALKY---EIYLNSSLVQFLLSRALGNI 1004

  Fly  1255 --SHQHLSCSRTSSCSSSQQGTSCRRKKGADVDGGCGDAGPHNSCSNVHSLGGLQFQEDDVWSQE 1317
              :| :|......:....|..|......||                 :.|:||.:.:|:.:...:
Human  1005 QIAH-NLYWLLKDALHDVQFSTRYEHVLGA-----------------LLSVGGKRLREELLKQTK 1051

  Fly  1318 EDEI---TAQYLNMRKVSERDTISQISLDSCDSRDQ--------GPPVVFNIGDVRLRHCSNLSC 1371
            ..::   .|:.:.....|.|..:.|.|::...|..|        .|.:|..  ::.::.||..|
Human  1052 LVQLLGGVAEKVRQASGSARQVVLQRSMERVQSFFQKNKCRLPLKPSLVAK--ELNIKSCSFFS- 1113

  Fly  1372 ENTKSFSNDPEDPSAAALK------EPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELM 1430
                  ||      |..||      :|..|:                  ::.:.|.|:||||:::
Human  1114 ------SN------AVPLKVTMVNADPMGEE------------------INVMFKVGEDLRQDML 1148

  Fly  1431 ATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIE--PILNTVSLHQIKKNSNKSLKEYFIDE 1493
            |.|::::...||.:|.:||.:..:|.:....|.|::|  |..:|:...|::.....|.|:..:.|
Human  1149 ALQMIKIMDKIWLKEGLDLRMVIFKCLSTGRDRGMVELVPASDTLRKIQVEYGVTGSFKDKPLAE 1213

  Fly  1494 Y---GSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISPK 1555
            :   .:||.|.:.:|.:||:.|||..|:.:|:|.:.||||.||:..|.||:.|||||..|..:..
Human  1214 WLRKYNPSEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLRSTGHMFHIDFGKFLGHAQM 1278

  Fly  1556 NLGF--EQSPFKLTPEFVEVMGGTSSE--HWREFNKLLLVGMMSARKHMDRIINFVEIMRSNAHL 1616
            ...|  :::||.||.:...|:.|....  .::.|..|........||..:..:|.:.:|     :
Human  1279 FGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQAYNLIRKQTNLFLNLLSLM-----I 1338

  Fly  1617 PCFKNGCSG-----TVQNL---RKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGI 1673
            |      ||     ::|:|   |.......|:.|......:|::.||.|::||    :.::.:.:
Human  1339 P------SGLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIESSLGSIATK----FNFFIHNL 1393

  Fly  1674 LXERF 1678
            ...||
Human  1394 AQLRF 1398

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
fwd	NP_001356971.1	PI4Kc_III_beta	1385..1674	CDD:270712	82/311 (26%)
PIK3C2A	NP_001308307.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..61
		Interaction with clathrin, sufficient to induce clathrin assembly. /evidence=ECO:0000269|PubMed:16215232	2..142
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	348..367		3/18 (17%)
		PI3K_rbd	409..512	CDD:279173	23/133 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	618..639		5/34 (15%)
		C2A_PI3K_class_II	677..849	CDD:175979	48/301 (16%)
		PI3Ka_II	870..1038	CDD:238441	34/210 (16%)
		PI3Kc_C2_alpha	1043..1395	CDD:270720	97/399 (24%)
		PX_PI3K_C2_alpha	1426..1534	CDD:132822
		Interaction with PtdIns(4,5)P2-containing membranes. /evidence=ECO:0000250|UniProtKB:Q61194	1488..1493
		C2B_PI3K_class_II	1560..1681	CDD:176027
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:11606566	1608..1619

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5032
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.810