DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment fwd and Pik3c2a

DIOPT Version :9

Sequence 1:	NP_001356971.1	Gene:	fwd / 45374	FlyBaseID:	FBgn0004373	Length:	1682	Species:	Drosophila melanogaster
Sequence 2:	NP_035213.2	Gene:	Pik3c2a / 18704	MGIID:	1203729	Length:	1686	Species:	Mus musculus

Alignment Length:	1577	Identity:	314/1577 - (19%)
Similarity:	556/1577 - (35%)	Gaps:	442/1577 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly   219 CEPHSASTTTLSSL-------GSDLGNGNGSTVPAFLMAAPVTLPIGGQMP--TIKCLPGASSAA 274
            |.|   ||.||.|.       .:...||....:|.|.....|.|.:.||.|  :....|
Mouse   147 CGP---STYTLPSTYPSAYSKQATFQNGFSPRMPTFPSTESVYLRLPGQSPYFSYPLTP------ 202

  Fly   275 STPKHSRYS----KPRLS--LSRVFNHSMPS----VHGRPNLSLAPAGVPGSVNPPKRISTHQRN 329
            :||.|.:.|    :|.:|  ::::|.....:    .:|:....|..|....||          .|
Mouse   203 ATPFHPQGSLPVYRPLVSPDMAKLFEKIASTSEFLKNGKARTDLEIANSKASV----------CN 257

  Fly   330 LSLDFRSMGIL------LPPVSQVTNNHINHTQHHRNRSLDSALQRIP----EVEVSSPNAESEN 384
            |.:..:|..|.      |.|:|:...:::...:|...:.....|...|    .:|..||:...|.
Mouse   258 LQISPKSEDINKFDWLDLDPLSKPKVDYVEVLEHEEEKKDPVLLAEDPWDAVLLEERSPSCHLER 322

  Fly   385 TFCSPSILGATM--CSKIATTAATVTSALSPPGTPTPALMSTACTPTIGAVKGNVQHPVPKHEQS 447
            .....|:.|||:  ...:....|..|.|........|  ..|:..||..::....:      .|:
Mouse   323 KVNGKSLSGATVTRSQSLIIRTAQFTKAQGQVSQKDP--NGTSSLPTGSSLLQEFE------VQN 379

  Fly   448 ISLAAACRSSAVVESCPTLRMRPKSSESVAANGQEMIGVIVSSTGNGSSSSNNSCSSAGSTKKRE 512
            ..:||.|:|        .::::.|...:......   |.::|..    :...|.|....|.|...
Mouse   380 DEVAAFCQS--------IMKLKTKFPYTDHCTNP---GYLLSPV----TVQRNMCGENASVKVSI 429

  Fly   513 DLTSLGSDDSGIIC--GSESDQISLNRICHSHESL---DSGEMDADADAEEECVDLMDTTSIDEE 572
            ::..|....: ..|  .|..:.|.:..:|..|:.|   |.|........:||
Mouse   430 EIEGLQLPVT-FTCDVSSTVEIIIMQALCWVHDDLNQVDVGSYILKVCGQEE------------- 480

  Fly   573 YNIMQPDHLCLYPPQAASTELDCRTLRPSRKQKRQQQQVLERKNLPSQNQIESEDSVDAGVDA-R 636
              ::|.:| ||     .|.|    .::..||...:.:..|...:...||...:.:..:|.||. :
Mouse   481 --VLQNNH-CL-----GSHE----HIQNCRKWDTEIKLQLLTLSAMCQNLARTAEDDEAPVDLNK 533

  Fly   637 TRYRVMN-----MSQAAINVELGTASNEIDPDVNYRESHQ--DPIADAPSLSTPSVASTASATPS 694
            ..|::..     |::..:...|.:...:::..:.....|:  |.:..|       |....||...
Mouse   534 YLYQIEKPYKEVMTRHPVEELLDSYHYQVELALQTENQHRAVDQVIKA-------VRKICSALDG 591

  Fly   695 VTPTPIATPTPTESTKNDQVLFKNFFGATKNAIFRTAQSIIENHEKKNAAKQKEQPDHSGTVSLV 759
            |     .||:.||:.|.           .|.|:              |..:.|.    :...||.
Mouse   592 V-----ETPSVTEAVKK-----------LKRAV--------------NLPRNKS----ADVTSLS 622

  Fly   760 GSPNGGSSVPQDLVKSPTDISKKKEFFSLLTSSSSIKAAAAAAANSAASTPPATPTEALAANSNS 824
            ||.                           |..:|.|          .|..|..|.:.
Mouse   623 GSD---------------------------TRKNSTK----------GSLNPENPVQV------- 643

  Fly   825 KLQPLSDPSADLKVKERTLNLRLPGDSEGSEGALTKCS------SINSIHKLKLPVPGVVKYFIS 883
            .:..|:....||        |||..:|       ::||      |.|                |.
Mouse   644 SMDHLTTAIYDL--------LRLHANS-------SRCSTGCPRGSRN----------------IK 677

  Fly   884 EKAPVSDVLQKPEKGQSGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFS-FPDQEVDLY 947
            |....::.||.......|:...:.|     ::..:||..|     ||..|..:|. ...::|..|
Mouse   678 EAWTATEQLQFTVYAAHGISSNWVS-----NYEKYYLICS-----LSHNGKDLFKPIQSKKVGTY 732

  Fly   948 --------IPQLVVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNS--HN 1002
                    ..:|::..||:.:|                  .|:.:..|..:.....|.|:|  .|
Mouse   733 KNFFYLIKWDELIIFPIQISQL------------------PLESVLHLTLFGVLNQSSGSSPDSN 779

  Fly  1003 SSRKSKLALMKEIFSKREHKQTQNDLKSAGTGGLHV---GEPRSVV-ALAKKTHHRSQSDATVLL 1063
            ..||...||.|...:..:.|:    ..:.||..|::   ....|:. |:.||::   ..:..||.
Mouse   780 KQRKGPEALGKVSLTLFDFKR----FLTCGTKLLYLWTSSHTNSIPGAIPKKSY---VMERIVLQ 837

  Fly  1064 ADFRSPHTLSISHRMYQQPPYTTQTLPTTPAKLCLGDLTSGHAFDNGCTCFETVRGQVNGLLGQR 1128
            .||.|| ...|   :|..|......:.....:....|                ::|::..::.:.
Mouse   838 VDFPSP-AFDI---IYTSPQIDRNIIQQDKLETLESD----------------IKGKLLDIIHRD 882

  Fly  1129 TLCSCGAPKTSPQKEFM-----------KALMNVGKNLTSWPSKAEKTSALRMSLNLINKNLPAR 1182
            :  |.|..|  ..|.|:           ..|..:..:..:|     |.:.|..:.:|:::
Mouse   883 S--SFGLSK--EDKVFLWENRYYCLKHPNCLPKILASAPNW-----KWANLAKTYSLLHQ----- 933

  Fly  1183 VWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPKMMPSLRHTKSEEH 1247
             |.||   .|...:.:.:.|.|  :.:.::..:.::|.  |.|...:.|:|:.:.:|::   |.:
Mouse   934 -WPPL---CPLAALELLDAKFA--DQEVRSLAVSWMEA--ISDDELADLLPQFVQALKY---EIY 987

  Fly  1248 LDGSCLN------------SHQHLSCSRTSSCSSSQQGTSCRRKKGADVDGGCGDAGPHNSCSN- 1299
            |:.|.:.            :|. |......:...:..|:......||.:  ..|..|.....|.
Mouse   988 LNSSLVRFLLSRALGNIQIAHS-LYWLLKDALHDTHFGSRYEHVLGALL--SVGGKGLREELSKQ 1049

  Fly  1300 ---VHSLGGLQFQEDDVWSQEEDEITAQYLNMRKVSERDTISQISLDSCDS---RDQ-----GPP 1353
               |..|||:                |:.:.....|.|..:.|.|::...|   |::     .|.
Mouse  1050 MKLVQLLGGV----------------AEKVRQASGSTRQVVLQKSMERVQSFFLRNKCRLPLKPS 1098

  Fly  1354 VVFNIGDVRLRHCSNLSCENTKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAI 1418
            :|..  ::.::.||..|       ||      |..||......:.|..|            ::.:
Mouse  1099 LVAK--ELNIKSCSFFS-------SN------AMPLKVTMVNADPLGEE------------INVM 1136

  Fly  1419 VKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIE--PILNTVSLHQIKKN 1481
            .|.|:||||:::|.|::::...||.:|.:||.:..::.:....|.|::|  |..:|:...|::..
Mouse  1137 FKVGEDLRQDMLALQMIKIMDKIWLKEGLDLRMVIFRCLSTGRDRGMVELVPASDTLRKIQVEYG 1201

  Fly  1482 SNKSLKEYFIDEY---GSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIH 1543
            ...|.|:..:.|:   .:||.|.:.:|.:||:.|||..|:.:|:|.:.||||.||:..|.||:.|
Mouse  1202 VTGSFKDKPLAEWLRKYNPSEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLRSTGHMFH 1266

  Fly  1544 IDFGFILSISPKNLGF--EQSPFKLTPEFVEVMGGTSSE--HWREFNKLLLVGMMSARKHMDRII 1604
            ||||..|..:.....|  :::||.||.:...|:.|....  .::.|..|........||..:..:
Mouse  1267 IDFGKFLGHAQMFGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQAYNLIRKQTNLFL 1331

  Fly  1605 NFVEIMRSNAHLPCFKNGCSG-----TVQNL---RKRFHMNLTEQEMERKVEQLVQDSLKSLSTK 1661
            |.:.:|     :|      ||     ::|:|   |.......|:.|......:|::.||.|::||
Mouse  1332 NLLSLM-----IP------SGLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIESSLGSIATK 1385

  Fly  1662 LYDGYQYYTNGILXERF 1678
                :.::.:.:...||
Mouse  1386 ----FNFFIHNLAQLRF 1398

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
fwd	NP_001356971.1	PI4Kc_III_beta	1385..1674	CDD:270712	81/305 (27%)
Pik3c2a	NP_035213.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..26
		Interaction with clathrin, sufficient to induce clathrin assembly. /evidence=ECO:0000250\|UniProtKB:O00443	2..142
		ETS_PEA3_N	<40..>210	CDD:368026	20/71 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	41..68
		PI3K_rbd	421..513	CDD:366310	22/117 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	615..640		9/65 (14%)
		C2A_PI3K_class_II	677..849	CDD:175979	44/210 (21%)
		PI3Ka_II	869..1038	CDD:238441	32/212 (15%)
		PI3Kc_C2_alpha	1043..1395	CDD:270720	100/409 (24%)
		PX_PI3K_C2_alpha	1426..1534	CDD:132822
		Interaction with PtdIns(4,5)P2-containing membranes. /evidence=ECO:0000269\|PubMed:16338929	1488..1493
		C2B_PI3K_class_II	1560..1681	CDD:176027
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:O00443	1606..1617
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5032
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810