DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment fwd and piki-1

DIOPT Version :9

Sequence 1:	NP_001356971.1	Gene:	fwd / 45374	FlyBaseID:	FBgn0004373	Length:	1682	Species:	Drosophila melanogaster
Sequence 2:	NP_510529.1	Gene:	piki-1 / 181618	WormBaseID:	WBGene00009552	Length:	1607	Species:	Caenorhabditis elegans

Alignment Length:	1654	Identity:	302/1654 - (18%)
Similarity:	535/1654 - (32%)	Gaps:	586/1654 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly   191 SPSVSQNNTSSATGRMS------------SPHSQSSLSIACEPHSASTTTLSSLGSDLGNGNGST 243
            ||..|..:.|.||..:.            .|||||......:..||..:|             |.
Worm    57 SPGPSSYSGSLATSPIDFRPVYNEPRAGPIPHSQSYPRNYFQDWSAIAST-------------SQ 108

  Fly   244 VPAFLMAAPVTLPIGGQMPTIKCLPGASSAASTPKHSRYSKPRLSLSRVFNHSMPSVHGR----- 303
            .|||   .|...|                    ||..:|..|...       |:|.:|.|
Worm   109 PPAF---PPPPRP--------------------PKPEQYKFPPAP-------SVPLLHDRYFVPP 143

  Fly   304 -----PNLSLAPAGVPGSVNP-PKRISTHQRNLSLDFRS-MGILLPPVS--QVTNNHINHTQHHR 359
                 |..|......|..::| |...||..|:.:..|.| ....|.|:.  :::.|....|..::
Worm   144 PPPVPPRHSRVQQSPPVPIHPTPPVSSTPLRHSAPSFASDSQQFLSPIKPFEISFNSTVDTSSNQ 208

  Fly   360 NRSLDSALQRIPEVEVSSPNA-ESENTFCSPSILGATMCSKIATTAATVTSALSPPGTP----TP 419
            ..|.|.::|..|...:..|.. .|.|     |..||.:...:...:|...|:.:.....    .|
Worm   209 TGSHDHSIQYQPLTHLYVPYVMHSLN-----SSYGALLNGDLIDLSAFEDSSNASQDEIRKEFDP 268

  Fly   420 ALMSTACTPT------IGAVKGNVQHPVPKHE---------QSISLAAACRSSAVVESCPTLRMR 469
            ..:||..|.|      :.||......|:.:.|         ::|...:   ||..:..||
Worm   269 LFISTYSTDTPSPDNSMPAVNAYFSKPIDEPECVGGAKLIQENIEFPS---SSFCLIDCP----- 325

  Fly   470 PKSSESVAANGQEMIGVIVSSTGNGSSSSNNSCSSAGSTKK-------REDLTS----LGSDDSG 523
                     ||.|                       ...||       |:|:|.    ..:.|..
Worm   326 ---------NGIE-----------------------EQVKKLCKRNLIRKDMTPDFFIAPTVDYM 358

  Fly   524 IICGSESDQISLNRICHSHESLDSGEMDADA--DAEEECVDLMDTTSI---------DEEYNI-- 575
            :...|     ::..:.:...|..:.:.:..|  .|.:|.:|::.|.::         |:||.:
Worm   359 VTTAS-----TVKVVVYKDHSWKANKSNGKAMICAIDEKMDIITTQALSLFDSELPTDKEYGLKI 418

  Fly   576 ------MQPDHL---------CLYPPQAASTELDCRTLRPSRKQKRQQQQVLERKNLPSQNQIES 625
                  :..|.|         ||.  .....:||.....|:   .|..:|.||..|| .::|::.
Worm   419 YGLNQFLSSDSLLGSNLYTGHCLL--NGDDVKLDLGVFAPN---SRIYEQTLESWNL-MKSQVKY 477

  Fly   626 EDSVD-AGVDARTRYRVMNMSQAAI------NVELGTASNEID--------------PDVNYRES 669
            ...|| ..|:....:....|||..|      .::|.::|..:.              |:..|.|.
Worm   478 STVVDKEDVENTLGHLASEMSQYEIAFNDGSTLKLSSSSQRVKQVIMLLCKCLHGIVPEKLYNEM 542

  Fly   670 HQDPIADAPSLSTPSVASTASATPSVTPTPIATPTPTESTKNDQVLFKNFFGATKNAIFRTAQSI 734
             |..:|                                ||..||::..      :|...|...|.
Worm   543 -QKYLA--------------------------------STTEDQLVHH------RNDFLREIHSF 568

  Fly   735 IENHEKKNAAKQKEQPDHSGTVSLVGSPNGGSSVPQDLVKSP-TDISKKKEFFSLLTSSSSIKAA 798
            :|.:.:             .|||....|      |..::..| .::..|.:|..::
Worm   569 LELYCR-------------CTVSRYNIP------PLQIITKPKVEVLSKMDFLQIM--------- 605

  Fly   799 AAAAANSAASTPPATP---------------TEALAANSNSKLQPLSDP------SADLKVKERT 842
                .||..|.|....               |:.|...||...:.:.:.      ..||..|.:.
Worm   606 ----LNSVHSIPEHWQSQYSEFYMSLDLYHGTQVLDGFSNKVPKTIKNDHFFPRIPLDLYAKFKR 666

  Fly   843 LNL-RLPGDSEGSEGALTKCSSINSIHKLKLPVPGVVK---YFISEKAPVSDVLQKPEKGQSGLL 903
            ||| :.|.::                 ::.:.:.|.|:   ...:|..|  |::         :|
Worm   667 LNLCQYPRET-----------------RIVVSISGTVRNSAQAANEYNP--DIV---------ML 703

  Fly   904 RFFESPVF--NIHFAVHYLF-----YSKEP-----GVLSFIGNK-----IFSFPDQEVDLYIPQL 951
            .:...|::  |:......||     ..|:|     |...:|.:.     |.||...:.::|.|.:
Worm   704 GYCSVPLYDENLFMRQGPLFLPLTLLKKQPMLKPFGPYPYIKDARDPILIMSFKIWDTEIYFPNV 768

  Fly   952 VVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNSHNSSRKSKLALMKEIF 1016
            |:      ::..:...:.|:.              :|...|.::.:.|...|:.::
Worm   769 VI------DMQCIPQDFATLD--------------IETQEYLLELIENQDTSTLET--------- 804

  Fly  1017 SKREHKQTQNDLKSAGTGGLHVGEPRSVVALAKKTHHRSQSDATVLLADFRSPHTLSISHRMYQ- 1080
                   ...||                 ...|:.|..:|.:|..|:.......:.....|:||
Worm   805 -------DDQDL-----------------IWQKRLHLTNQPEALPLVLSSLQDWSFGFVMRVYQI 845

  Fly  1081 -------QPPYTTQ-TLPTTPAKLCLGDLTSGHAFDNGCTCFETVRGQVNGLLGQRTLCSCGAPK 1137
                   :|....: .||..|     .:....||..:      ..||..:.|.           .
Worm   846 LEEWAPLRPEIAMEFLLPKYP-----DERIRAHAVQS------LARGSTDFLY-----------H 888

  Fly  1138 TSPQKEFMKALMNVGKNLTSWPSKAEKTSALR---MSLNLINKNLPARVWLPLYSDIPHHVVRIT 1199
            |.||  |::||.          .:..:.|||.   :.|:.::.:....::..|...:.|..|
Worm   889 TIPQ--FIEALR----------FELYEKSALADFILELSFVSLDFTFEIYWQLQQRVDHCAV--- 938

  Fly  1200 EEKTAVLNSKDKTPYIIYVEVVE--IPDIYTSPLIPKMMPSLRHTKSEEHLDGSCLNSHQHLSCS 1262
                      |..||.|..:.::  :.|.:.:|          :.|::..|....||.
Worm   939 ----------DDLPYAIRCQNLQQKMIDEHENP----------NLKTDIKLQHELLNE------- 976

  Fly  1263 RTSSCSSSQQGTSCRRKKGADVDGGCGDAGPHNSCSNVHSLGGLQFQEDDVWSQEEDEITAQYLN 1327
                                                       |...:||:.|:..|....:...
Worm   977 -------------------------------------------LDSIQDDLRSKSGDSEIERLHR 998

  Fly  1328 MRK----VSERDTISQISLDSCDSRDQGPPVVFNIGDVRLRHCSNLSCENTKSFSNDPEDPSAAA 1388
            :|.    :..:...:::.|..|.:        |:...||:..||..: .|.|..
Worm   999 LRTRLGILDSKLLQNKVRLPICPA--------FDCTGVRIEECSVFN-SNAKPL----------- 1043

  Fly  1389 LKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRP 1453
                     |::..    |...|:   |.|.|..||:||:....::|.....||:...:||.:..
Worm  1044 ---------KIVFR----GLNMNY---SIIHKRDDDMRQDAFVMKMLNEMDRIWKSNGLDLRMIT 1092

  Fly  1454 YKIVCLSNDSGLIEPILNTVSLHQIKKNS-----------NKSLKEYFIDEYGSPSGESFRRAQK 1507
            ::|:.:....|:.|.:||..:|.:|:|..           .|.|.::..||:      :::.||:
Worm  1093 FRIMPVGYRRGMGELVLNCATLMEIQKEEGLRGVLNDEILRKWLMKHNSDEF------AYKEAQE 1151

  Fly  1508 NFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISPKNLGF--EQSPFKLTPEF 1570
            ||::|||.:|:::|:|.:.||||.||||..:||:.|||||..:.......||  ::.||..|.|.
Worm  1152 NFIRSCAGWCIVTYVLGIGDRHNDNILFTKNGHVFHIDFGKYMGDWQMAAGFRRDRVPFVFTTEM 1216

  Fly  1571 VEVM--GGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRSNAHLPCFKNGCSGTVQNLRKR 1633
            ..|:  |...:::.::|...........|::.:.:.|.:.|| :.:.:|.........|:|   .
Worm  1217 FHVINNGRAPTQYNQKFIDYCCKAFNHLRRNKNTLTNLLRIM-ACSDIPGINMDSLAFVEN---N 1277

  Fly  1634 FHMNLTEQEMERKVEQLVQDSLKSLSTKL 1662
            ..::|::.:...:...::|:||.|...:|
Worm  1278 LMLDLSDTDATVQFTAMIQNSLGSAFVRL 1306

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
fwd	NP_001356971.1	PI4Kc_III_beta	1385..1674	CDD:270712	74/293 (25%)
piki-1	NP_510529.1	PI3K_rbd	348..456	CDD:197540	17/114 (15%)
		C2A_PI3K_class_II	593..775	CDD:175979	37/228 (16%)
		PI3Ka_II	784..948	CDD:238441	40/243 (16%)
		PI3Kc_II	964..1315	CDD:270710	93/439 (21%)
		PX_domain	1346..1454	CDD:295365
		C2B_PI3K_class_II	1473..1604	CDD:176027
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5032
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810