Protein Alignment na and Scn1a

DIOPT Version :10

• Sequence 1: NP_001096981.2
• Gene: na / 45338
• FlyBaseID: FBgn0002917
• Length: 2233
• Species: Drosophila melanogaster
• Sequence 2: NP_110502.2
• Gene: Scn1a / 81574
• RGDID: 69364
• Length: 2009
• Species: Rattus norvegicus
• Alignment Length: 2144
• Identity: 429/2144 - (20%)
• Similarity: 767/2144 - (35%)
• Gaps: 673/2144 - (31%)

  Fly   328 LKGGE--PFLADYG--PEESLN---ESADIEWVNKLWVRRLMRLCALVSLTSVSLNTPKTFERYP 385
            |:.|:  ||:  ||  |.|.::   |..|..::||              .|.:.||..|...|:.
  Rat    55 LEAGKNLPFI--YGDIPPEMVSEPLEDLDPYYINK--------------KTFIVLNKGKAIFRFS 103

  Fly   386 SLQ-------------------------------------FITFAS--------DTAVTLLFTAE 405
            :..                                     |:|.::        :...|.::|.|
  Rat   104 ATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFE 168

  Fly   406 MIAKMHIRGVLHGEVPYLKDHWCQFDASMVSFLWISIILQIFEVLEIVPKFSYLSIMRAPRPLIM 470
            .:.|:..||....:..:|:|.|...|.::::|.:::             :|..|..:.|.|...:
  Rat   169 SLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVT-------------EFVDLGNVSALRTFRV 220

  Fly   471 IRFLRVFLKFSMPKSRINQIFKRSSQQIYNVTLFFLFFMSLYGLLGVQ-FFGELKNHCVM----- 529
            :|.|:........|:.:..:. :|.:::.:|.:..:|.:|::.|:|:| |.|.|:|.||.
  Rat   221 LRALKTISVIPGLKTIVGALI-QSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTN 284

  Fly   530 -NNTEYDLYKRPILTINSLAIPDT--------------------------FCSMDPDSGYQCSPG 567
             :..|:.:.|......|...:.:|                          .|....|:| ||..|
  Rat   285 ASLEEHSIEKNVTTDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAG-QCPEG 348

  Fly   568 MVCMKMDFLSSYVIGFNGFEDIATSIFTVYQAASQEGWVFIMYRAIDSLPAWRAA-----FYFST 627
            .:|:|.....:|  |:..|:..:.:..::::..:|:.|..:....:      |||     .:|..
  Rat   349 YMCVKAGRNPNY--GYTSFDTFSWAFLSLFRLMTQDFWENLYQLTL------RAAGKTYMIFFVL 405

  Fly   628 MIFFLAWLVKNVFIAVITETFNE-----------IRVQFQQMW----------------GARGHI 665
            :||..::.:.|:.:||:...:.|           ...:||||.                .|..|.
  Rat   406 VIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAATASEHS 470

  Fly   666 QKTAASQILSGNDT--------------------------------------------------- 679
            ::.:|:..||.:.:
  Rat   471 REPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFR 535

  Fly   680 ----GWRLV----------------------------------------------------TIDD 688
                |.||.                                                    |.:|
  Rat   536 FSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFED 600

  Fly   689 N-----------KHG------------------GL-------APETCHAIL---------RSPYF 708
            |           :||                  ||       :...|:.::         .||..
  Rat   601 NESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAGLPANGKMHSTVDCNGVVSLVGGPSVPTSPVG 665

  Fly   709 RMLVMSVILA-----NGIVTAT-------------MTFKHDGRPR-------------------- 735
            ::|...:|..     ||..|.|             |.|..|...|
  Rat   666 QLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEES 730

  Fly   736 ------------------------------------DVF-----------------YERYYYIE- 746
                                                |.|                 .|.|...|
  Rat   731 RQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEH 795

  Fly   747 ---------LVFTCLLDLETLFKIYCLGWRGYYKHSIHKFELLLAAGTTLHIVPMFYP-----SG 797
                     ||||.:...|...||..:....|::...:.|:..:   .||.:|.:...     |.
  Rat   796 FNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFI---VTLSLVELGLANVEGLSV 857

  Fly   798 LTYFQVLRVVRLIKASPMLEGFVYKIFGPG-KKLGSLIIFTMCLLIISSSISMQLF--------C 853
            |..|::|||.:|.|:.|.| ..:.||.|.. ..||:|.:....::.|.:.:.||||        |
  Rat   858 LRSFRLLRVFKLAKSWPTL-NMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVC 921

  Fly   854 FL---CDFTKF--ESFPEAFMSMFQILTQEAWVEVMDETMIRTSKTLTPLVAVYFILYHLFVTLI 913
            .:   |...::  ..|..:|:.:|::|..| |:|.|.:.|....:.:...|   |::..:...|:
  Rat   922 KIATDCKLPRWHMNDFFHSFLIVFRVLCGE-WIETMWDCMEVAGQAMCLTV---FMMVMVIGNLV 982

  Fly   914 VLSLFVAVIL-----DNLELDEDIKKLKQL-----KFREQSAEIKETLPFRLRIFEKFPDSPQMT 968
            ||:||:|::|     |||...:|..::..|     :..:..|.:|.      :|:|
  Rat   983 VLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKR------KIYE--------- 1032

  Fly   969 ILHRIPNDFMLPKVRESFMKHFVI--ELETEDSL---VENCKRPMSECWESNVVFRKQKP---VR 1025
                        .:::||::...|  |::..|.|   .:||        .||......|.   ::
  Rat  1033 ------------FIQQSFVRKQKILDEIKPLDDLNNRKDNC--------TSNHTTEIGKDLDCLK 1077

  Fly  1026 IMNKTAKVRAAGSSLRKLAITHIINDSNNQRLMLGDSAMLPVVGTKGGGGLKSQGTITHSKPWRV 1090
            .:|.|......|||:.|    :||::|:....:...|..:.|....|....::..|
  Rat  1078 DVNGTTSGIGTGSSVEK----YIIDESDYMSFINNPSLTVTVPIAVGESDFENLNT--------- 1129

  Fly  1091 DQKKFGSRSIRRSVRSGSIKLKQTYEHLMENGDIAAAPRANSGRARPHDLDIKLLQAKRQQAEMR 1155
              :.|.|.|          .|:::.|.|.|:...:.....:.|            ....:|..|
  Rat  1130 --EDFSSES----------DLEESKEKLNESSSSSEGSTVDIG------------APAEEQPVM- 1169

  Fly  1156 RNQREEDLRENHPFFDTPLFLVPRESRFRKICQKIVHARYDARLKDPLTGKE----RKVQYKSL- 1215
              :.||.|.....|.:..:      .|| |.||..|.   :.|      ||:    |:..::.:
  Rat  1170 --EPEETLEPEACFTEGCV------QRF-KCCQISVE---EGR------GKQWWNLRRTCFRIVE 1216

  Fly  1216 HNFLGLVTYLDWVMIFATTLSCISMMFET--PNYRVMDHPTLQIAEYGFVIFMSLELALKILADG 1278
            ||      :.:..::|...||..::.||.  .:.|......|:.|:..|.....||:.||.:|.|
  Rat  1217 HN------WFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYG 1275

  Fly  1279 LFFTPKAYIKDVAAALDVFIYVVSTSFLCWMPLNIPTNSAAQLLMILRCVRPLRIFTLVPHMRKV 1343
            .    :.|..:....||..|..||...|....|......|.:.|..||.:||||..:....||.|
  Rat  1276 Y----QTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVV 1336

  Fly  1344 VYELCRGFKEILLVSTLLILLMFIFASYGVQLYGGRLARCNDPT---------ISRREDCVGVFM 1399
            |..|......|:.|..:.::...||:..||.|:.|:...|.:.|         ::...||:.:..
  Rat  1337 VNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDTFEITEVNNHSDCLKLIE 1401

  Fly  1400 RRVFVTKMKLTPGPDESYPAMLVPRVWANPRRFNFDNIGDAMLTLFEVLSFKGWLDV-------R 1457
            |.....                    |.|. :.||||:|...|:|.:|.:||||:|:       |
  Rat  1402 RNETAR--------------------WKNV-KVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSR 1445

  Fly  1458 DVLIKAVGPVH--AVYIHIY----IFLGCMIGLTLFVGVVIANYSENK-----GTALLTVDQRRW 1511
            :|.::   |.:  ::|:::|    |..|....|.||:||:|.|:::.|     ....:|.:|:::
  Rat  1446 NVELQ---PKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKY 1507

  Fly  1512 CDLKKRLKIAQPLHLPPRPDGRKIRAFTYDITQHIIFKRVIAVVVLINSMLLSI-TWIKGEVHTE 1575
            .:..|:|...:|....||| |.|.:...:|.....:|...|.:::.:|.:.:.: |..:.:..|.
  Rat  1508 YNAMKKLGSKKPQKPIPRP-GNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTS 1571

  Fly  1576 RLVIVSAVLTFVFVVEVVMKNIAFTPRGY-----WQSRRNRYDLLVTVAGVIWIILQTILRNDLS 1635
            .|..::.|...:|..|.|:|.|:.  |.|     |    |.:|.:|.:..::.:.|..::.   .
  Rat  1572 ILSRINLVFIVLFTGECVLKLISL--RHYYFTIGW----NIFDFVVVILSIVGMFLAELIE---K 1627

  Fly  1636 YFFG---FMVVIL----RFFTITGKHTTLKMLMLTVGVSVCKSFFIIFGMFLLVFFYALAGTILF 1693
            ||..   |.|:.|    |...:......::.|:..:.:|:...|.|...:||::|.||:.|...|
  Rat  1628 YFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNF 1692

  Fly  1694 GTVKYGEGIGRRANFGSPVTGVAMLFRIVTGEDWNKIMHDCM-VQPPYC-----TLGNNYWETDC 1752
            ..||...||....||.:....:..||:|.|...|:.::...: .:||.|     ..|::. :.||
  Rat  1693 AYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSV-KGDC 1756

  Fly  1753 GNFTASLIYFCTFYVIITYIVLNLLVAIIMENFSLFYSNEEDA-LLSYADIRNFQNTWNIVDIHQ 1816
            ||.:..:.:|.::.:|...:|:|:.:|:|:||||:  :.||.| .||..|...|...|...|...
  Rat  1757 GNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSV--ATEESAEPLSEDDFEMFYEVWEKFDPDA 1819

  Fly  1817 RGVIPVRRVKFILRLLKGRLECDPQKDRLLFKYMCYEL---DKLHNGEDVTFHDVINMLSYRSVD 1878
            ...:...::......|:..|.. ||.::|....|...:   |::| ..|:.|.....:|......
  Rat  1820 TQFMEFEKLSQFAAALEPPLNL-PQPNKLQLIAMDLPMVSGDRIH-CLDILFAFTKRVLGESGEM 1882

  Fly  1879 IRKALQLEELL-----AREEFEYLV------EEEVAKMTI-RTWLEGCLKK-IRAQNASKQQNSL 1930
            ....:|:||..     ::..::.:.      :|||:.:.| |.:....||: ::..:.:..:|.|
  Rat  1883 DALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKL 1947

  Fly  1931 IAGLRATNEQPVMRPNIQEDKAPLGAVDKSAISTISGAVAGACPPTSDAFS-PTFSSTENEEKD 1993
            ..|.....::.::...|.|:     ::.:....|:|.|   ||||:.|..: |.....|.|.||
  Rat  1948 KGGANLLVKEDMIIDRINEN-----SITEKTDLTMSTA---ACPPSYDRVTKPIVEKHEQEGKD 2003

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
na	NP_001096981.2	Ion_trans	361..654	CDD:459842	65/386 (17%)
		Ion_trans	705..927	CDD:459842	72/346 (21%)
		Ion_trans	1224..1497	CDD:459842	78/296 (26%)
		Ion_trans	1545..1793	CDD:459842	65/266 (24%)
Scn1a	NP_110502.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	28..60		2/4 (50%)
		I. /evidence=ECO:0000305	110..454		64/366 (17%)
		Ion_trans	127..433	CDD:459842	60/328 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	458..528		5/69 (7%)
		Na_trans_cytopl	555..718	CDD:463401	20/162 (12%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	584..628		5/43 (12%)
		II. /evidence=ECO:0000305	750..1022		63/279 (23%)
		Ion_trans	767..980	CDD:459842	51/220 (23%)
		Na_trans_assoc	1006..1213	CDD:461936	53/297 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1129..1163		9/66 (14%)
		III. /evidence=ECO:0000305	1200..1514		88/353 (25%)
		Ion_trans	1217..1492	CDD:459842	81/308 (26%)
		Na_channel_gate	1484..1536	CDD:240441	12/52 (23%)
		IV. /evidence=ECO:0000305	1523..1821		80/310 (26%)
		Ion_trans	1540..1796	CDD:459842	66/267 (25%)
		S1-S2 loop of repeat IV. /evidence=ECO:0000250|UniProtKB:A2APX8	1561..1571		1/9 (11%)
		S3b-S4 loop of repeat IV. /evidence=ECO:0000250|UniProtKB:A2APX8	1619..1636		3/19 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1986..2009		6/18 (33%)