Protein Alignment na and nalcn

DIOPT Version :9

• Sequence 1: NP_001096981.2
• Gene: na / 45338
• FlyBaseID: FBgn0002917
• Length: 2233
• Species: Drosophila melanogaster
• Sequence 2: XP_005167755.1
• Gene: nalcn / 548345
• ZFINID: ZDB-GENE-050410-12
• Length: 1743
• Species: Danio rerio
• Alignment Length: 1768
• Identity: 972/1768 - (54%)
• Similarity: 1212/1768 - (68%)
• Gaps: 116/1768 - (6%)

  Fly   321 MLGRKQSLKGGEPFLADYGPEESLNESADIEWVNKLWVRRLMRLCALVSLTSVSLNTPKTFERYP 385
            ||.||||.:.....:.|:||:|:|.:||||.|:||.||..|:|.||::|:.||.:|||||||.||
Zfish    26 MLKRKQSSRVEAQPMTDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYP 90

  Fly   386 SLQFITFASDTAVTLLFTAEMIAKMHIRGVLHGEVPYLKDHWCQFDASMVSFLWISIILQIFEVL 450
            .||::|||.||.:..|:||||||||||||::.||..|:||.||.||..||.|||:|::||:||:.
Zfish    91 PLQYVTFALDTLLMFLYTAEMIAKMHIRGIIKGENSYVKDRWCMFDGFMVVFLWVSLVLQVFEIA 155

  Fly   451 EIVPKFSYLSIMRAPRPLIMIRFLRVFLKFSMPKSRINQIFKRSSQQIYNVTLFFLFFMSLYGLL 515
            |:|.:.|...::|.||.|||||..|::.:|.:|:|||..|.|||.:||::||:|.|||:.|||:|
Zfish   156 ELVDQMSPWGMLRIPRALIMIRVFRIYFRFELPRSRITNILKRSGEQIWSVTIFLLFFLLLYGIL 220

  Fly   516 GVQFFGELKNHCVMNNTEYDLYKRPILTINSLAIPDTFCSMDPDSGYQCSPGMVCMKM-DF-LSS 578
            |||.||...:|||.|.||     :..:|.||||||||.||.:.: ||||..|..|:.: |: ||.
Zfish   221 GVQMFGTFNHHCVTNETE-----KEKVTWNSLAIPDTHCSPNGE-GYQCPVGFKCVDLEDYGLSR 279

  Fly   579 YVIGFNGFEDIATSIFTVYQAASQEGWVFIMYRAIDSLPAWRAAFYFSTMIFFLAWLVKNVFIAV 643
            ..:|::||.::.|||||||:|||||||||||||||||.|.||:.|||.|:|||||||||||||||
Zfish   280 QELGYSGFNELGTSIFTVYEAASQEGWVFIMYRAIDSFPRWRSYFYFLTLIFFLAWLVKNVFIAV 344

  Fly   644 ITETFNEIRVQFQQMWGARGHIQKTAASQIL----SGNDTGWRLVTIDDNKHGGLAPETCHAILR 704
            |.|||.|||||||||||:|.....||.:|:.    ||   ||:||.:|.||..|.||.....::|
Zfish   345 IIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDASG---GWQLVAVDVNKPHGRAPACLQQLMR 406

  Fly   705 SPYFRMLVMSVILANGIVTATMTFKHDGRPRDVFYERYYYIELVFTCLLDLETLFKIYCLGWRGY 769
            |..|.|.::|::..:.||.|:...:.|.:   :..:.:|..|:.||.|.|||.|.||:|||:.||
Zfish   407 SSVFHMFILSMVAVDVIVAASNYHRGDHK---LTNDEFYLAEVAFTVLFDLEALLKIWCLGFTGY 468

  Fly   770 YKHSIHKFELLLAAGTTLHIVPMFYPSGLTYFQVLRVVRLIKASPMLEGFVYKIFGPGKKLGSLI 834
            ...|:||||.||..||||||.|..|.|..|||||||||||||.||.||.|||||||||||||||:
Zfish   469 ISSSLHKFESLLVVGTTLHIYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLV 533

  Fly   835 IFTMCLLIISSSISMQLFCFLCDFTKFESFPEAFMSMFQILTQEAWVEVMDETMIRTSKTLTPLV 899
            :||..|||:.|:||:|:|||:.|..:|.:||.||||||||||||.||:|||:|::...:...|:|
Zfish   534 VFTASLLIVMSAISLQMFCFVEDLDRFTTFPRAFMSMFQILTQEGWVDVMDQTLVAVGQMWAPVV 598

  Fly   900 AVYFILYHLFVTLIVLSLFVAVILDNLELDEDIKKLKQLKFREQSAEIKETLPFRLRIFEKFPDS 964
            |:||||||||.|||:|||||||||||||||||:|||||||..|.:|:.||.||.|||||||||:.
Zfish   599 AIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNR 663

  Fly   965 PQMTILHRIPNDFMLPKVRESFMKHFVIELETEDSLV---------ENCKRPMSECWESNVVFRK 1020
            |||..:.::|:||.:|::||||||.|:...:.:.|.:         .:|........|.|    |
Zfish   664 PQMVKISKLPSDFTVPRIRESFMKQFIDRQQQDPSCLFRRLPSASSSSCDHSKRSAIEDN----K 724

  Fly  1021 QKPVRIMNKTAKVRAAGSSLRKLAITHIINDSNNQRLMLGDSAMLPVVGTKGGGGLKSQGTITHS 1085
            ....::......:||.....:::.:..|:.....||::.|.....|   ||....|..|..|
Zfish   725 YIDQKLRKSVFSIRARNLMEKEITVNKILRACTRQRMLSGSFEGQP---TKERSILSVQHHI--- 783

  Fly  1086 KPWRVDQKKFGSRSIRRSVRSGS----IKLKQTYEHLMENGDIAAAPRANSGRARPHDLDIKLLQ 1146
                        |..|||:|.||    |...::.|.|.::.......|    .|:..|.:||::|
Zfish   784 ------------RQERRSLRHGSTSQRISRGKSLETLTQDHSSTVRYR----NAQREDSEIKMIQ 832

  Fly  1147 AKRQQAEMRRNQREEDLRENHPFFDTPLFLVPRESRFRKICQKIVHARYDARLKDPLTGKERKVQ 1211
            .|::||||:|..:||:||||||:||.|||:|.||.|||..|:.||.||::|...||.||.....:
Zfish   833 EKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRMIVRARFNASNTDPNTGAVNSTK 897

  Fly  1212 YKSLHNFLGLVTYLDWVMIFATTLSCISMMFETPNYRVMDHPTLQIAEYGFVIFMSLELALKILA 1276
            |..|::.||||||||||||..|..||||||||:|..|||..|||||.||.||||||:||.|||:|
Zfish   898 YHQLYDLLGLVTYLDWVMIVVTICSCISMMFESPFTRVMHVPTLQIGEYVFVIFMSIELNLKIMA 962

  Fly  1277 DGLFFTPKAYIKDVAAALDVFIYVVSTSFLCWMPLNIPTNSAAQLLMILRCVRPLRIFTLVPHMR 1341
            |||||||.|.|:|....:|:|||:||..||||:|.|:|..|.|||||:|||.||||||.|||.||
Zfish   963 DGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWLPNNVPPESGAQLLMMLRCFRPLRIFKLVPQMR 1027

  Fly  1342 KVVYELCRGFKEILLVSTLLILLMFIFASYGVQLYGGRLARCNDPTISRREDCVGVFMRRVFVTK 1406
            |||.|:.:|||||.|||.||:.||.:||::||||:.|:||:||||.||.::||.|:|...|.::|
Zfish  1028 KVVREVLKGFKEIFLVSILLLTLMLVFATFGVQLFAGKLAKCNDPHISSKDDCHGIFRINVSISK 1092

  Fly  1407 ---MKLTPGPDESYPAMLVPRVWANPRRFNFDNIGDAMLTLFEVLSFKGWLDVRDVLIKAVGPVH 1468
               :||..|  |..|...|||||||||.|||||:|:|||.||||||.|||::||||:|..|||:|
Zfish  1093 NLNLKLKDG--EKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIH 1155

  Fly  1469 AVYIHIYIFLGCMIGLTLFVGVVIANYSENKGTALLTVDQRRWCDLKKRLKIAQPLHLPPRPDGR 1533
            .:|||:::||||||||||||||||||::|||||||||||||||.|||.||||||||||||||:..
Zfish  1156 GIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPENG 1220

  Fly  1534 KIRAFTYDITQHIIFKRVIAVVVLINSMLLSITWIKGEVHTERLVIVSAVLTFVFVVEVVMKNIA 1598
            ..||..||||||..|||.|||:||..|:|||:.|.:....|..|..:|.|.||:||:||.||.||
Zfish  1221 GFRAKMYDITQHPFFKRGIAVLVLAQSVLLSVKWDELGAVTFPLATMSVVFTFIFVLEVTMKLIA 1285

  Fly  1599 FTPRGYWQSRRNRYDLLVTVAGVIWIILQTILRNDLSYFFGFMVVILRFFTITGKHTTLKMLMLT 1663
            .:|.|||||||||||||||..|:|||||...|:|..:|..|..|::.|||||.|||.|||||:||
Zfish  1286 MSPAGYWQSRRNRYDLLVTSLGLIWIILHFALQNAYTYMMGTCVIVFRFFTICGKHVTLKMLLLT 1350

  Fly  1664 VGVSVCKSFFIIFGMFLLVFFYALAGTILFGTVKYGEGIGRRANFGSPVTGVAMLFRIVTGEDWN 1728
            |.||:.||||||.|||||:..||.||.:|||||||||.|.|.|||.:....:.:|.|||||||||
Zfish  1351 VVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSTAGKAITVLIRIVTGEDWN 1415

  Fly  1729 KIMHDCMVQPPYCTLG-NNYWETDCGNFTASLIYFCTFYVIITYIVLNLLVAIIMENFSLFYSNE 1792
            |||||||||||:||.. :.||||||||:..:|||||:|||||.||:||||||||:||||||||.|
Zfish  1416 KIMHDCMVQPPFCTPDKHRYWETDCGNYAGALIYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTE 1480

  Fly  1793 EDALLSYADIRNFQNTWNIVDIHQRGVIPVRRVKFILRLLKGRLECDPQKDRLLFKYMCYELDKL 1857
            ||.||||.|:|:||..||:||..:.||||..||||:||||:||||.|..||:||||:||||:::|
Zfish  1481 EDQLLSYNDLRHFQIIWNMVDDKREGVIPTSRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERL 1545

  Fly  1858 HNGEDVTFHDVINMLSYRSVDIRKALQLEELLAREEFEYLVEEEVAKMTIRTWLEGCLKKIRAQN 1922
            |||.|||||||::|||||||||||:||||||||||:.||.:||||||.|||.||:.|||:|||:
Zfish  1546 HNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAK- 1609

  Fly  1923 ASKQQNSLIAGLRATNEQPVMR----PNIQ-----ED------KAPLGAVDKSAISTISGAVAGA 1972
             .:|..|:|..||.:.:|.:.|    |:|:     ||      ..|......|.:.|:
Zfish  1610 -QQQSCSIILSLRESQQQDLRRLLNPPSIETTVPSEDTNTHNQDNPTQPEQNSGLQTL------- 1666

  Fly  1973 CPPTSDAFSPTFSSTENEEKDGSSS-----AVVQLPHSETSI-------------AGTGSSA--- 2016
                   .|||.|......:|.:..     ...:||...||:             |.:||..
Zfish  1667 -------LSPTLSDRSGYRQDSADRPQRKLGQWRLPAGRTSVKSMVCKMNPVTDEASSGSEVKKW 1724

  Fly  2017 -TGAATATGTSSG 2028
             |...|.....||
Zfish  1725 WTRQLTVESDESG 1737

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
na	NP_001096981.2	Ion_trans	373..654	CDD:278921	169/282 (60%)
		Ion_trans	734..927	CDD:278921	123/192 (64%)
		Ion_trans	1240..1497	CDD:278921	169/259 (65%)
		Ion_trans	1571..1793	CDD:278921	147/222 (66%)
nalcn	XP_005167755.1	Ion_trans	85..355	CDD:278921	164/275 (60%)
		Ion_trans	429..626	CDD:278921	124/199 (62%)
		Ion_trans	926..1184	CDD:278921	169/259 (65%)
		Ion_trans	1263..1481	CDD:278921	146/217 (67%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170577750
Domainoid	1	1.000	377	1.000	Domainoid score	I816
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H21832
Inparanoid	1	1.050	1838	1.000	Inparanoid score	I60
OMA	1	1.010	-	-		QHG48327
OrthoDB	1	1.010	-	-		D16416at33208
OrthoFinder	1	1.000	-	-		FOG0004135
OrthoInspector	1	1.000	-	-		oto39806
orthoMCL	1	0.900	-	-		OOG6_104443
Panther	1	1.100	-	-	LDO	PTHR46141
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R175
SonicParanoid	1	1.000	-	-		X3959
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	15	14.940