Protein Alignment na and Scn1a

DIOPT Version :10

• Sequence 1: NP_001096981.2
• Gene: na / 45338
• FlyBaseID: FBgn0002917
• Length: 2233
• Species: Drosophila melanogaster
• Sequence 2: NP_001300926.1
• Gene: Scn1a / 20265
• MGIID: 98246
• Length: 2009
• Species: Mus musculus
• Alignment Length: 2150
• Identity: 424/2150 - (19%)
• Similarity: 761/2150 - (35%)
• Gaps: 685/2150 - (31%)

  Fly   328 LKGGE--PFLADYG--PEESLN---ESADIEWVNKLWVRRLMRLCALVSLTSVSLNTPKTFERYP 385
            |:.|:  ||:  ||  |.|.::   |..|..::||              .|.:.||..|...|:.
Mouse    55 LEAGKNLPFI--YGDIPPEMVSEPLEDLDPYYINK--------------KTFIVLNKGKAIFRFS 103

  Fly   386 SLQ-------------------------------------FITFAS--------DTAVTLLFTAE 405
            :..                                     |:|.::        :...|.::|.|
Mouse   104 ATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFE 168

  Fly   406 MIAKMHIRGVLHGEVPYLKDHWCQFDASMVSFLWISIILQIFEVLEIVPKFSYLSIMRAPRPLIM 470
            .:.|:..||....:..:|:|.|...|.::::|.:::             :|..|..:.|.|...:
Mouse   169 SLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVT-------------EFVDLGNVSALRTFRV 220

  Fly   471 IRFLRVFLKFSMPKSRINQIFKRSSQQIYNVTLFFLFFMSLYGLLGVQ-FFGELKNHCVM----- 529
            :|.|:........|:.:..:. :|.:::.:|.:..:|.:|::.|:|:| |.|.|:|.||.
Mouse   221 LRALKTISVIPGLKTIVGALI-QSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTN 284

  Fly   530 -NNTEYDLYKRPILTINSLAIPDT--------------------------FCSMDPDSGYQCSPG 567
             :..|:.:.|...:..|...:.:|                          .|....|:| ||..|
Mouse   285 ASLEEHSIEKNITMDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAG-QCPEG 348

  Fly   568 MVCMKMDFLSSYVIGFNGFEDIATSIFTVYQAASQEGWVFIMYRAIDSLPAWRAA-----FYFST 627
            .:|:|.....:|  |:..|:..:.:..::::..:|:.|..:....:      |||     .:|..
Mouse   349 YMCVKAGRNPNY--GYTSFDTFSWAFLSLFRLMTQDFWENLYQLTL------RAAGKTYMIFFVL 405

  Fly   628 MIFFLAWLVKNVFIAVITETFNE-----------IRVQFQQM----------------WGARGHI 665
            :||..::.:.|:.:||:...:.|           ...:||||                ..|..|.
Mouse   406 VIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAATTASEHS 470

  Fly   666 QKTAASQILSGNDT--------------------------------------------------- 679
            ::.:|:..||.:.:
Mouse   471 REPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFR 535

  Fly   680 ----GWRLV----------------------------------------------------TIDD 688
                |.||.                                                    |.:|
Mouse   536 FSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFED 600

  Fly   689 N-----------KHGGLAPETCHAILRSPYFRMLVM--------SVILANGIVT----------- 723
            |           :||...........||.  |||.:        |.:..||:|:
Mouse   601 NESRRDSLFVPRRHGERRNSNLSQTSRSS--RMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSP 663

  Fly   724 -----------------------------------ATMTFKHDGRPR------------------ 735
                                               .:|.|..|...|
Mouse   664 VGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELE 728

  Fly   736 --------------------------------------DVF-----------------YERYYYI 745
                                                  |.|                 .|.|...
Mouse   729 ESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMT 793

  Fly   746 E----------LVFTCLLDLETLFKIYCLGWRGYYKHSIHKFELLLAAGTTLHIVPMFYP----- 795
            |          ||||.:...|...||..:....|::...:.|:..:   .||.:|.:...
Mouse   794 EHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFI---VTLSLVELGLANVEGL 855

  Fly   796 SGLTYFQVLRVVRLIKASPMLEGFVYKIFGPG-KKLGSLIIFTMCLLIISSSISMQLF------- 852
            |.|..|::|||.:|.|:.|.| ..:.||.|.. ..||:|.:....::.|.:.:.||||
Mouse   856 SVLRSFRLLRVFKLAKSWPTL-NMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDC 919

  Fly   853 -CFL---CDFTKF--ESFPEAFMSMFQILTQEAWVEVMDETMIRTSKTLTPLVAVYFILYHLFVT 911
             |.:   |...::  ..|..:|:.:|::|..| |:|.|.:.|....:.:...|   |::..:...
Mouse   920 VCKIATDCKLPRWHMNDFFHSFLIVFRVLCGE-WIETMWDCMEVAGQAMCLTV---FMMVMVIGN 980

  Fly   912 LIVLSLFVAVIL-----DNLELDEDIKKLKQL-----KFREQSAEIKETLPFRLRIFEKFPDSPQ 966
            |:||:||:|::|     |||...:|..::..|     :..:..|.:|.      :|:|
Mouse   981 LVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGIAYVKR------KIYE------- 1032

  Fly   967 MTILHRIPNDFMLPKVRESFMKHFVI--ELETEDSL---VENCKRPMSECWESNVVFRKQKPVRI 1026
                          .:::||:|...|  |::..|.|   .:||   :|.  .:..:.:....::.
Mouse  1033 --------------FIQQSFVKKQKILDEIKPLDDLNNRKDNC---ISN--HTTEIGKDLDCLKD 1078

  Fly  1027 MNKTAKVRAAGSSLRKLAITHIINDSNNQRLMLGDSAMLPVVGTKGGGGLKSQGTITHSKPWRVD 1091
            :|.|......|||:.|    :||::|:....:...|..:.|....|....::..|
Mouse  1079 VNGTTSGIGTGSSVEK----YIIDESDYMSFINNPSLTVTVPIAVGESDFENLNT---------- 1129

  Fly  1092 QKKFGSRSIRRSVRSGSIKLKQTYEHLMENGDIAAAPRANSGRARPHDLDIKLLQAKRQQAEMRR 1156
             :.|.|.|          .|:::.|.|.|:...:.....:.|..
Mouse  1130 -EDFSSES----------DLEESKEKLNESSSSSEGSTVDIGAP--------------------- 1162

  Fly  1157 NQREEDLRENHPFFDTPLFLVPRE-------SRFRKICQKIVHARYDARLKDPLTGKE----RKV 1210
                   .|..|..:....|.|..       .|| |.||..|.   :.|      ||:    |:.
Mouse  1163 -------AEEQPVIEPEETLEPEACFTEGCVQRF-KCCQISVE---EGR------GKQWWNLRRT 1210

  Fly  1211 QYKSL-HNFLGLVTYLDWVMIFATTLSCISMMFET--PNYRVMDHPTLQIAEYGFVIFMSLELAL 1272
            .::.: ||      :.:..::|...||..::.||.  .:.|......|:.|:..|.....||:.|
Mouse  1211 CFRIVEHN------WFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLL 1269

  Fly  1273 KILADGLFFTPKAYIKDVAAALDVFIYVVSTSFLCWMPLNIPTNSAAQLLMILRCVRPLRIFTLV 1337
            |.:|.|.    :.|..:....||..|..||...|....|......|.:.|..||.:||||..:..
Mouse  1270 KWVAYGY----QTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRF 1330

  Fly  1338 PHMRKVVYELCRGFKEILLVSTLLILLMFIFASYGVQLYGGRLARCNDPT---------ISRRED 1393
            ..||.||..|......|:.|..:.::...||:..||.|:.|:...|.:.|         ::...|
Mouse  1331 EGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDIFEISEVNNHSD 1395

  Fly  1394 CVGVFMRRVFVTKMKLTPGPDESYPAMLVPRVWANPRRFNFDNIGDAMLTLFEVLSFKGWLDV-- 1456
            |:.:..|.....                    |.|. :.||||:|...|:|.:|.:||||:|:
Mouse  1396 CLKLIERNETAR--------------------WKNV-KVNFDNVGFGYLSLLQVATFKGWMDIMY 1439

  Fly  1457 -----RDVLIKAVGPVH--AVYIHIY----IFLGCMIGLTLFVGVVIANYSENK-----GTALLT 1505
                 |:|.::   |.:  ::|:::|    |..|....|.||:||:|.|:::.|     ....:|
Mouse  1440 AAVDSRNVELQ---PKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMT 1501

  Fly  1506 VDQRRWCDLKKRLKIAQPLHLPPRPDGRKIRAFTYDITQHIIFKRVIAVVVLINSMLLSI-TWIK 1569
            .:|:::.:..|:|...:|....||| |.|.:...:|.....:|...|.:::.:|.:.:.: |..:
Mouse  1502 EEQKKYYNAMKKLGSKKPQKPIPRP-GNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQ 1565

  Fly  1570 GEVHTERLVIVSAVLTFVFVVEVVMKNIAFTPRGY-----WQSRRNRYDLLVTVAGVIWIILQTI 1629
            .:..|..|..::.|...:|..|.|:|.|:.  |.|     |    |.:|.:|.:..::.:.|..:
Mouse  1566 SDYVTSILSRINLVFIVLFTGECVLKLISL--RHYYFTIGW----NIFDFVVVILSIVGMFLAEL 1624

  Fly  1630 LRNDLSYFFG---FMVVIL----RFFTITGKHTTLKMLMLTVGVSVCKSFFIIFGMFLLVFFYAL 1687
            :.   .||..   |.|:.|    |...:......::.|:..:.:|:...|.|...:||::|.||:
Mouse  1625 IE---KYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAI 1686

  Fly  1688 AGTILFGTVKYGEGIGRRANFGSPVTGVAMLFRIVTGEDWNKIMHDCM-VQPPYC-----TLGNN 1746
            .|...|..||...||....||.:....:..||:|.|...|:.::...: .:||.|     ..|::
Mouse  1687 FGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSS 1751

  Fly  1747 YWETDCGNFTASLIYFCTFYVIITYIVLNLLVAIIMENFSLFYSNEEDA-LLSYADIRNFQNTWN 1810
            . :.||||.:..:.:|.::.:|...:|:|:.:|:|:||||:  :.||.| .||..|...|...|.
Mouse  1752 V-KGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSV--ATEESAEPLSEDDFEMFYEVWE 1813

  Fly  1811 IVDIHQRGVIPVRRVKFILRLLKGRLECDPQKDRLLFKYMCYEL---DKLHNGEDVTFHDVINML 1872
            ..|......:...::......|:..|.. ||.::|....|...:   |::| ..|:.|.....:|
Mouse  1814 KFDPDATQFMEFEKLSQFAAALEPPLNL-PQPNKLQLIAMDLPMVSGDRIH-CLDILFAFTKRVL 1876

  Fly  1873 SYRSVDIRKALQLEELL-----AREEFEYLV------EEEVAKMTI-RTWLEGCLKK-IRAQNAS 1924
            ..........:|:||..     ::..::.:.      :|||:.:.| |.:....||: ::..:.:
Mouse  1877 GESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFT 1941

  Fly  1925 KQQNSLIAGLRATNEQPVMRPNIQEDKAPLGAVDKSAISTISGAVAGACPPTSDAFS-PTFSSTE 1988
            ..:|.|..|.....::.::...|.|:     ::.:....|:|.|   ||||:.|..: |.....|
Mouse  1942 YNKNKLKGGANLLVKEDMLIDRINEN-----SITEKTDLTMSTA---ACPPSYDRVTKPIVEKHE 1998

  Fly  1989 NEEKD 1993
            .|.||
Mouse  1999 QEGKD 2003

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
na	NP_001096981.2	Ion_trans	361..654	CDD:459842	65/386 (17%)
		Ion_trans	705..927	CDD:459842	72/382 (19%)
		Ion_trans	1224..1497	CDD:459842	78/296 (26%)
		Ion_trans	1545..1793	CDD:459842	65/266 (24%)
Scn1a	NP_001300926.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	28..60		2/4 (50%)
		I. /evidence=ECO:0000250|UniProtKB:P04774	110..454		64/366 (17%)
		Ion_trans	127..433	CDD:459842	60/328 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	455..528		5/72 (7%)
		Na_trans_cytopl	555..718	CDD:463401	18/164 (11%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	584..627		5/42 (12%)
		II. /evidence=ECO:0000250|UniProtKB:P04774	750..1022		63/279 (23%)
		Ion_trans	767..980	CDD:459842	51/220 (23%)
		Na_trans_assoc	1006..1213	CDD:461936	50/301 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1129..1163		9/82 (11%)
		III. /evidence=ECO:0000250|UniProtKB:P04774	1200..1514		88/353 (25%)
		Ion_trans	1217..1492	CDD:459842	81/308 (26%)
		Na_channel_gate	1484..1536	CDD:240441	12/52 (23%)
		IV. /evidence=ECO:0000250|UniProtKB:P04774	1523..1821		80/310 (26%)
		Ion_trans	1540..1796	CDD:459842	66/267 (25%)
		S1-S2 loop of repeat IV. /evidence=ECO:0000269|PubMed:27281198	1561..1571		1/9 (11%)
		S3b-S4 loop of repeat IV. /evidence=ECO:0000269|PubMed:27281198	1619..1636		3/19 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1984..2009		6/19 (32%)