Protein Alignment kn and Ebf2

DIOPT Version :9

• Sequence 1: NP_001097317.1
• Gene: kn / 45318
• FlyBaseID: FBgn0001319
• Length: 689
• Species: Drosophila melanogaster
• Sequence 2: NP_001263316.1
• Gene: Ebf2 / 13592
• MGIID: 894332
• Length: 575
• Species: Mus musculus
• Alignment Length: 600
• Identity: 353/600 - (58%)
• Similarity: 399/600 - (66%)
• Gaps: 124/600 - (20%)

  Fly   154 MFGLPPTAAVDMNQPRGPMTSLKEEPLGSR------WAMQ-PVVD-----QSNLGIGRAHFEKQP 206
            |||:..|..      |||  :||::.||:.      |... .|||     ||.:.:.||||||||
Mouse     1 MFGIQDTLG------RGP--ALKDKSLGAEMDSVRSWVRNVGVVDANVAAQSGVALSRAHFEKQP 57

  Fly   207 PSNLRKSNFFHFVIALYDRAGQPIEIERTAFIGFIEKDSESDATKTNNGIQYRLQLLYANGARQE 271
            |||||||||||||:|||||.|||:|||||||:.|:|.|.|....|||||..|:|||||:||.|.|
Mouse    58 PSNLRKSNFFHFVLALYDRQGQPVEIERTAFVDFVENDKEQGNEKTNNGTHYKLQLLYSNGVRTE 122

  Fly   272 QDIFVRLIDSVTKQAIIYEGQDKNPEMCRVLLTHEVMCSRCCDKKSCGNRNETPSDPVIIDRFFL 336
            ||::||||||||||.|.||||:||||||||||||||||||||:||||||||||||||||||||||
Mouse   123 QDLYVRLIDSVTKQPIAYEGQNKNPEMCRVLLTHEVMCSRCCEKKSCGNRNETPSDPVIIDRFFL 187

  Fly   337 KFFLKCNQNCLKNAGNPRDMRRFQVVISTQVAVDGPLLAISDNMFVHNNSKHGRRAKRLDTTEGT 401
            ||||||||||||.|||||||||||||:||.|.|||.:||:||||||||||||||||:|||.:|
Mouse   188 KFFLKCNQNCLKTAGNPRDMRRFQVVLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSE-- 250

  Fly   402 GNTSLSISGHPLAPDSTYDGLYPPLPVATPCIKAISPSEGWTTGGATVIIVGDNFFDGLQVVFGT 466
                                       |||||||||||||||||||.|||:||||||||||||||
Mouse   251 ---------------------------ATPCIKAISPSEGWTTGGAMVIIIGDNFFDGLQVVFGT 288

  Fly   467 MLVWSELITSHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGSPGRFVYVSALNEPTIDYGFQRLQK 531
            |||||||||.||||||||||||||||||||||||||||||:||||:| :||||||||||||||||
Mouse   289 MLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFIY-TALNEPTIDYGFQRLQK 352

  Fly   532 LIPRHPGDPEKLQKEIILKRAADLV----------------------EALYSMPRSP-------- 566
            :|||||||||:|.||::||||||||                      |||||:||:|
Mouse   353 VIPRHPGDPERLAKEMLLKRAADLVEALYGTPHNNQDIILKRAADIAEALYSVPRNPSQIPALSS 417

  Fly   567 ----GGSTGFNSYAGQLAVSVQDGSGQWTEDDYQRAQSSSVSPRGGYCSSASTPHSSGGSYGATA 627
                .|..|.|||..||.||:.: |.|.....|.| .:||:||||  .||:|||..|  :|..::
Mouse   418 SPAHSGMMGINSYGSQLGVSISE-STQGNNQGYIR-NTSSISPRG--YSSSSTPQQS--NYSTSS 476

  Fly   628 ASAAVAATANGYA--PAPNMGT------------------LSSSPGSVFNSTSRVSSLSFNPFA- 671
            .|      .|||:  |..|:|.                  :||||....:|||.:...|.:.|.
Mouse   477 NS------MNGYSNVPMANLGVPGSPGFLNGSPTGSPYGIMSSSPTVGSSSTSSILPFSSSVFPA 535

  Fly   672 -------LPTCNTQG 679
                   .|....||
Mouse   536 VKQKSAFAPVIRPQG 550

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
kn	NP_001097317.1	COE_DBD	184..395	CDD:212156	169/216 (78%)
		IPT_COE	431..515	CDD:238580	78/83 (94%)
		COE1_HLH	517..560	CDD:293032	37/64 (58%)
Ebf2	NP_001263316.1	COE1_DBD	20..246	CDD:406750	170/225 (76%)
		Interaction with DNA. /evidence=ECO:0000250	62..65		2/2 (100%)
		Interaction with DNA. /evidence=ECO:0000250	196..203		5/6 (83%)
		Interaction with DNA. /evidence=ECO:0000250	235..238		2/2 (100%)
		IPT_COE	253..337	CDD:238580	78/84 (93%)
		COE1_HLH	338..381	CDD:406751	35/42 (83%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	441..479		18/48 (38%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167849482
Domainoid	1	1.000	374	1.000	Domainoid score	I868
eggNOG	1	0.900	-	-		E1_KOG3836
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	648	1.000	Inparanoid score	I789
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D352969at33208
OrthoFinder	1	1.000	-	-		FOG0000764
OrthoInspector	1	1.000	-	-		otm43377
orthoMCL	1	0.900	-	-		OOG6_102533
Panther	1	1.100	-	-	O	PTHR10747
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X577
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.760