DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hsp70Ba and HSPA5

DIOPT Version :9

Sequence 1:	NP_731716.1	Gene:	Hsp70Ba / 44921	FlyBaseID:	FBgn0013277	Length:	641	Species:	Drosophila melanogaster
Sequence 2:	NP_005338.1	Gene:	HSPA5 / 3309	HGNCID:	5238	Length:	654	Species:	Homo sapiens

Alignment Length:	640	Identity:	385/640 - (60%)
Similarity:	491/640 - (76%)	Gaps:	28/640 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 IGIDLGTTYSCVGVYQHGKVEIIANDQGNRTTPSYVAFT-DSERLIGDPAKNQVAMNPRNTVFDA 67
            :|||||||||||||:::|:|||||||||||.|||||||| :.||||||.||||:..||.||||||
Human    31 VGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDA 95

  Fly    68 KRLIGRKYDDPKIAEDMKHWPFKVVSDGGKPKIGVEY-KGESKRFAPEEISSMVLTKMKETAEAY 131
            ||||||.::||.:.:|:|..|||||....||.|.|:. .|::|.|||||||:|||||||||||||
Human    96 KRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAY 160

  Fly   132 LGESITDAVITVPAYFNDSQRQATKDAGHIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFD 196
            ||:.:|.||:||||||||:|||||||||.||||||:||||||||||:||||||. :||:|:|:||
Human   161 LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKR-EGEKNILVFD 224

  Fly   197 LGGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVTHLAEEFKRKYKKDLRSNPRALRRL 261
            |||||||||:||||.| :|||.:|.|||||||||||.|::.|..:.:|:|..||:|.:.||:::|
Human   225 LGGGTFDVSLLTIDNG-VFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKL 288

  Fly   262 RTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEKALNDAKMDK 326
            |...|:|||.|||..:|.|||::.:||:||...::||:||||..||||:|::||:|.|.|:.:.|
Human   289 RREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKK 353

  Fly   327 GQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLL 391
            ..|.:||||||||||||:|.|::|||:||..:..|||||||||||||||.:|||||.  ..|::|
Human   354 SDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQD--TGDLVL 416

  Fly   392 VDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTYSDNQPGVSIQVYEGERAMTKDNNALGT 456
            :||.||:|||||.||||||||.||..:|.|:::.|||.|||||.|:|:||||||.:||||:.|||
Human   417 LDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGT 481

  Fly   457 FDLSGIPPAPRGVPQIEVTFDLDANGILNVSAKEMSTGKAKNITIKNDKGRLSQAEIDRMVNEAE 521
            |||:||||||||||||||||::|.||||.|:|::..||....|||.||:.||:..||:||||:||
Human   482 FDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAE 546

  Fly   522 KYADEDEKHRQRITSRNALESYVYNVKQSV--EQAPAGKLDEADKNSVLDKCNETIRWLDSNTTA 584
            |:|:||:|.::||.:||.||||.|::|..:  ::...|||...||.::.....|.|.||:|:..|
Human   547 KFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDA 611

  Fly   585 EKEEFDHKMEELARHCSPIMTKMHQQGAGAAGGPGANCGQQAGGFGGYSGPTVEE 639
            :.|:|..|.:||.....||::|::    |:||.|                ||.||
Human   612 DIEDFKAKKKELEEIVQPIISKLY----GSAGPP----------------PTGEE 646

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hsp70Ba	NP_731716.1	PTZ00009	2..641	CDD:240227	385/640 (60%)
Hsp70Ba	NP_731716.1	HSPA1-2_6-8-like_NBD	3..379	CDD:212675	249/376 (66%)
HSPA5	NP_005338.1	Required for interaction with KIAA1324. /evidence=ECO:0000269\|PubMed:26045166	1..80		39/48 (81%)
		HSP70	30..635	CDD:394970	377/607 (62%)
		Nucleotide-binding (NBD). /evidence=ECO:0000303\|PubMed:28286085	125..280		111/156 (71%)
		Interdomain linker. /evidence=ECO:0000250\|UniProtKB:G3I8R9	409..419		3/11 (27%)
		Substrate-binding (SBD). /evidence=ECO:0000303\|PubMed:28286085	420..500		58/79 (73%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	633..654		9/34 (26%)
		Prevents secretion from ER. /evidence=ECO:0000255	651..654
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53894
OrthoDB	1	1.010	-	-		D288077at2759
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100083
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	6	5.820