Protein Alignment Hsp70Ab and Hspa1b

DIOPT Version :9

• Sequence 1: NP_524798.2
• Gene: Hsp70Ab / 44920
• FlyBaseID: FBgn0013276
• Length: 642
• Species: Drosophila melanogaster
• Sequence 2: NP_034608.2
• Gene: Hspa1b / 15511
• MGIID: 99517
• Length: 642
• Species: Mus musculus
• Alignment Length: 649
• Identity: 491/649 - (75%)
• Similarity: 555/649 - (85%)
• Gaps: 21/649 - (3%)

  Fly     3 AIGIDLGTTYSCVGVYQHGKVEIIANDQGNRTTPSYVAFTDSERLIGDPAKNQVAMNPRNTVFDA 67
            |||||||||||||||:|||||||||||||||||||||||||:||||||.||||||:||:||||||
Mouse     6 AIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDA 70

  Fly    68 KRLIGRKYDDPKIAEDMKHWPFKVVSDGGKPKIGVEYKGESKRFAPEEISSMVLTKMKETAEAYL 132
            |||||||:.|..:..|||||||:||:||.|||:.|.|||||:.|.||||||||||||||.|||||
Mouse    71 KRLIGRKFGDAVVQSDMKHWPFQVVNDGDKPKVQVNYKGESRSFFPEEISSMVLTKMKEIAEAYL 135

  Fly   133 GESITDAVITVPAYFNDSQRQATKDAGHIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFDL 197
            |..:|:|||||||||||||||||||||.|||||||||||||||||:|||||:..|||||||||||
Mouse   136 GHPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDL 200

  Fly   198 GGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVTHLADEFKRKYKKDLRSNPRALRRLR 262
            |||||||||||||:| :|||::|||||||||||||||||:|..:|||||:|||:..|.||:||||
Mouse   201 GGGTFDVSILTIDDG-IFEVKATAGDTHLGGEDFDNRLVSHFVEEFKRKHKKDISQNKRAVRRLR 264

  Fly   263 TAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEKALNDAKMDKG 327
            ||.|||||||||||:|::|||:||||.||||.::||||||||:||||.||:||||||.||||||.
Mouse   265 TACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRGTLEPVEKALRDAKMDKA 329

  Fly   328 QIHDIVLVGGSTRIPKVQSLLQDFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLLV 392
            ||||:|||||||||||||.||||||:|::||.|||||||||||||||||||.||:|..:||:||:
Mouse   330 QIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLL 394

  Fly   393 DVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTYADNQPGVSIQVYEGERAMTKDNNALGTF 457
            ||||||||:||||||||.||:||..||.|||:||:||:||||||.|||||||||||:|||.||.|
Mouse   395 DVAPLSLGLETAGGVMTALIKRNSTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRF 459

  Fly   458 DLSGIPPAPRGVPQIEVTFDLDANGILNVSAKEMSTGKAKNITIKNDKGRLSQAEIDRMVNEAEK 522
            :|||||||||||||||||||:||||||||:|.:.|||||..|||.|||||||:.||:|||.|||:
Mouse   460 ELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAER 524

  Fly   523 YADEDEKHRQRITSRNALESYVFNVKQAVE-QAPAGKLDEADKNSVLDKCNDTIRWLDSNTTAEK 586
            |..|||..|.|:.::||||||.||:|.||| :...|||.||||..|||||.:.|.||||||.|:|
Mouse   525 YKAEDEVQRDRVAAKNALESYAFNMKSAVEDEGLKGKLSEADKKKVLDKCQEVISWLDSNTLADK 589

  Fly   587 EEFDHKLEELTRHCSPIMTKMHQQGAGAGAGGPGANCGQQAGGFGGY--------SGPTVEEVD 642
            |||.||.|||.|.||||::.::|     |||.||      |||||..        ||||:||||
Mouse   590 EEFVHKREELERVCSPIISGLYQ-----GAGAPG------AGGFGAQAPPKGASGSGPTIEEVD 642

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hsp70Ab	NP_524798.2	PTZ00009	2..642	CDD:240227	489/647 (76%)
		HSPA1-2_6-8-like_NBD	3..379	CDD:212675	309/375 (82%)
Hspa1b	NP_034608.2	PTZ00009	1..642	CDD:240227	489/647 (76%)
		Nucleotide-binding domain (NBD). /evidence=ECO:0000250|UniProtKB:P11142	2..386		312/380 (82%)
		HSPA1-2_6-8-like_NBD	6..381	CDD:212675	309/375 (82%)
		Substrate-binding domain (SBD). /evidence=ECO:0000250|UniProtKB:P11142	394..509		91/114 (80%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	617..642		13/30 (43%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53894
OrthoDB	1	1.010	-	-		D180577at33208
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100083
Panther	1	1.100	-	-	O	PTHR19375
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	8	7.830