DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hsp70Ab and Hspa5

DIOPT Version :9

Sequence 1:	NP_524798.2	Gene:	Hsp70Ab / 44920	FlyBaseID:	FBgn0013276	Length:	642	Species:	Drosophila melanogaster
Sequence 2:	NP_001156906.1	Gene:	Hspa5 / 14828	MGIID:	95835	Length:	655	Species:	Mus musculus

Alignment Length:	620	Identity:	378/620 - (60%)
Similarity:	486/620 - (78%)	Gaps:	14/620 - (2%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 IGIDLGTTYSCVGVYQHGKVEIIANDQGNRTTPSYVAFT-DSERLIGDPAKNQVAMNPRNTVFDA 67
            :|||||||||||||:::|:|||||||||||.|||||||| :.||||||.||||:..||.||||||
Mouse    32 VGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDA 96

  Fly    68 KRLIGRKYDDPKIAEDMKHWPFKVVSDGGKPKIGVEY-KGESKRFAPEEISSMVLTKMKETAEAY 131
            ||||||.::||.:.:|:|..|||||....||.|.|:. .|::|.|||||||:|||||||||||||
Mouse    97 KRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAY 161

  Fly   132 LGESITDAVITVPAYFNDSQRQATKDAGHIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFD 196
            ||:.:|.||:||||||||:|||||||||.||||||:||||||||||:||||||. :||:|:|:||
Mouse   162 LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKR-EGEKNILVFD 225

  Fly   197 LGGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVTHLADEFKRKYKKDLRSNPRALRRL 261
            |||||||||:||||.| :|||.:|.|||||||||||.|::.|....:|:|..||:|.:.||:::|
Mouse   226 LGGGTFDVSLLTIDNG-VFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKL 289

  Fly   262 RTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEKALNDAKMDK 326
            |...|:|||.|||..:|.|||::.|||:||...::||:||||..||||:|::||:|.|.|:.:.|
Mouse   290 RREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKK 354

  Fly   327 GQIHDIVLVGGSTRIPKVQSLLQDFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLL 391
            ..|.:||||||||||||:|.|:::||:||..:..|||||||||||||||.:|||||.  ..|::|
Mouse   355 SDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQD--TGDLVL 417

  Fly   392 VDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTYADNQPGVSIQVYEGERAMTKDNNALGT 456
            :||.||:|||||.||||||||.||..:|.|:::.|||.:||||.|:|:||||||.:||||:.|||
Mouse   418 LDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGT 482

  Fly   457 FDLSGIPPAPRGVPQIEVTFDLDANGILNVSAKEMSTGKAKNITIKNDKGRLSQAEIDRMVNEAE 521
            |||:||||||||||||||||::|.||||.|:|::..||....|||.||:.||:..||:||||:||
Mouse   483 FDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAE 547

  Fly   522 KYADEDEKHRQRITSRNALESYVFNVKQAV--EQAPAGKLDEADKNSVLDKCNDTIRWLDSNTTA 584
            |:|:||:|.::||.:||.||||.:::|..:  ::...|||...||.::.....:.|.||:|:..|
Mouse   548 KFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDA 612

  Fly   585 EKEEFDHKLEELTRHCSPIMTKMHQQGAGAGAGGP 619
            :.|:|..|.:||.....||::|::      |:|||
Mouse   613 DIEDFKAKKKELEEIVQPIISKLY------GSGGP 641

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hsp70Ab	NP_524798.2	PTZ00009	2..642	CDD:240227	378/620 (61%)
Hsp70Ab	NP_524798.2	HSPA1-2_6-8-like_NBD	3..379	CDD:212675	249/376 (66%)
Hspa5	NP_001156906.1	Required for interaction with KIAA1324. /evidence=ECO:0000250\|UniProtKB:P11021	1..81		39/48 (81%)
		HSP70	31..636	CDD:365808	374/607 (62%)
		Nucleotide-binding (NBD). /evidence=ECO:0000250\|UniProtKB:P11021	126..281		111/156 (71%)
		Interdomain linker. /evidence=ECO:0000250\|UniProtKB:G3I8R9	410..420		3/11 (27%)
		Substrate-binding (SBD). /evidence=ECO:0000250\|UniProtKB:P11021	421..501		57/79 (72%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	632..655		5/16 (31%)
		Prevents secretion from ER	652..655
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53894
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100083
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.720