DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment kto and MED12

DIOPT Version :9

Sequence 1:	NP_524786.1	Gene:	kto / 44830	FlyBaseID:	FBgn0001324	Length:	2531	Species:	Drosophila melanogaster
Sequence 2:	NP_005111.2	Gene:	MED12 / 9968	HGNCID:	11957	Length:	2177	Species:	Homo sapiens

Alignment Length:	2692	Identity:	908/2692 - (33%)
Similarity:	1246/2692 - (46%)	Gaps:	702/2692 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 EKRPLKRTRLGPPDIYPQDAKQREDELTPTNVKHGFTTTPPLS-DEFGTAHNSNVNASKVSAFFS 70
            |.|||||.||||||:||||.||:|||||..|||.||...|.:| ||.|:|.|.:.|.:|:|:.||
Human    10 EHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFS 74

  Fly    71 GVLAKKEELMTLPDTGRKKQQINCKDNFWPVSPRRKCTVDAWFKDLAGNKPLLSLAKRAPSFNKK 135
            .::|:|....|||||||:|.|:|.|||||.|:.|.:..::.||.||||.|||..|||:.|.|:||
Human    75 SIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKK 139

  Fly   136 EEIFITLCENQVNMQRATWFIKLSAAYTLSFTESKNKKRSIYDPAAEWTGNMIKFMKELLPKLQE 200
            ||:|..|.:..|.:.||.|.||::.||..:.:|:|.|||.: ||..|||..:.|::.|.|.|:.|
Human   140 EEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHV-DPFMEWTQIITKYLWEQLQKMAE 203

  Fly   201 YYQQNHDKSSSNGTTSGSLTAAGNGPASNGSTGTSSINSVTGSSASTNVIPVPSMASPLPPIHSP 265
            ||:.                    |||.:|..|                    |...|||
Human   204 YYRP--------------------GPAGSGGCG--------------------STIGPLP----- 223

  Fly   266 ANGQQAAPGGGVNAGSVMPTTGSLGGVVGGPGSSVVGGAAGAGAAVPPGSTISGIGSQFEDSRNA 330
                                                                       .|...|
Human   224 -----------------------------------------------------------HDVEVA 229

  Fly   331 LKYWKYCHQLSKYMYEESLLDRQEFLNWILDLLDKMRTQASFDEPLKKLVLSFALQYMHDFVQSE 395
            ::.|.|..:|:.:|:::.:|||.|||.|:|:..:|:|..   ::.|.||:|...|:|..:||||.
Human   230 IRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPG---EDELLKLLLPLLLRYSGEFVQSA 291

  Fly   396 RLCRKMAYIVSKKLAQLL-------NTVVEQQTIKELDEPKLQQDPY----ELALQEQMSCPHHR 449
            .|.|::||..:::||..|       :.|:..|:...|......|.|.    .....:.:.||.||
Human   292 YLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHR 356

  Fly   450 DIVLYLSTILQIITIECPTALVWS-GIAAHRAPSSLLGSPLDHLPLAPSVLPMPTRCPRTNHEIR 513
            .:|..||.|||.|.:.||:||||. .:...|..:   ||||||||:|||.||||........::|
Human   357 PLVFGLSCILQTILLCCPSALVWHYSLTDSRIKT---GSPLDHLPIAPSNLPMPEGNSAFTQQVR 418

  Fly   514 RQLRAAESDIVLRTQHAEQRWFAAKWLSAGKNQYT--SVLATLDHLDTHCFDRMEHNNSIDTLYA 576
            .:||..|..|..|.|..|.||...|...|... :|  .||.||:.||:|.|:|.:.:||:|:|..
Human   419 AKLREIEQQIKERGQAVEVRWSFDKCQEATAG-FTIGRVLHTLEVLDSHSFERSDFSNSLDSLCN 482

  Fly   577 QIF---PSPTVSRRREEDQVEPRPPYEPKQDKD-TVRILCEWAVSGQRWGEHRAMVVAILLDKRQ 637
            :||   ||              :..:|...|.| .|.:|||||||.:|.|.|||||||.||:|||
Human   483 RIFGLGPS--------------KDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQ 533

  Fly   638 IDVTSTPADQQSSDKDDKDSLASGAGLIDGLPVFQHVLMHFLDHDAPVLDEHVSSPQQRTEFTNL 702
            .::.:.... :|...|:|.|:|||:......|:||.||:.|||..||:|.: ..|..:|.||.||
Human   534 AEIEAERCG-ESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTD-PRSESERVEFFNL 596

  Fly   703 VQLFSALIRHDVFSHNAYMHTLISRGDLLLESVLVIKSGTTATKTSPPPPAPPPTTTHGFDDDGF 767
            |.||..||||||||||.|..||||||||...:            ..|.||:|       |||...
Human   597 VLLFCELIRHDVFSHNMYTCTLISRGDLAFGA------------PGPRPPSP-------FDDPAD 642

  Fly   768 GGGLDFKHNEFDDS--------------NVDDDLDKLVQNIKE------------KGQQHEAPDS 806
                |.:|.|.:.|              ::|.....|.:::::            :|:...:|:.
Human   643 ----DPEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEK 703

  Fly   807 P----KIGPPGDGETNPGGSIS------RHYVYTKHFPIPQDDPSMSSYSSESNQRYILLFGVGK 861
            |    ::.||...:..  |::.      ||..|..||||||::    |.|.|.|||.::||||||
Human   704 PDVEKEVKPPPKEKIE--GTLGVLYDQPRHVQYATHFPIPQEE----SCSHECNQRLVVLFGVGK 762

  Fly   862 ERDEKKHAVKKMSKEIGKLFTKKFSIDV------------------AAAGHVKKHSRNEF--NFE 906
            :||:.:||:||::|:|.|:..:|.:.:.                  ...|..::.:|.|.  ..|
Human   763 QRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAE 827

  Fly   907 ATTSKCQQMAYFDQHVVTAQCAANVLEQLNGFALGNNNYLPVQEHVAFLFDLMELALNIYSLLEL 971
            ...:|.|.::::|||.||||.:.|||||:..||||.:.:||:.:||.|:|||||.:|:|..|::.
Human   828 DIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDF 892

  Fly   972 CDSLLKELPEVEHQLQLKKSNLVRSYTTSLALYIVSILRRYHSCLLLSPEQTLSVFEGVCRTIRH 1036
            ...||.||..||.:|.||.|:||.||||||.|.||::||.||:||:|:.:|...||||:|..::|
Human   893 AIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKH 957

  Fly  1037 VSNPSECTSAERCIIAYLSDLHESCVLLQGK--EQSTEYYQQLQCIKRFKDIFNTPEQLDLPPQG 1099
            ..|.|:.:||||||:|||.||:.||..|:.|  |..:::     |.|....|:...|    |.:.
Human   958 GMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDF-----CSKVKNTIYCNVE----PSES 1013

  Fly  1100 ---YNPLLLQELFMAPRRG-------GKLDPHWLGTLHESPANVYSFVSNALIAVC-RETDNERL 1153
               :.|..:.:....|...       ||       :|.|:|||.||||.|||:.|| ...|.:|:
Human  1014 NMRWAPEFMIDTLENPAAHTFTYTGLGK-------SLSENPANRYSFVCNALMHVCVGHHDPDRV 1071

  Fly  1154 NDVALACAELTASCNVLSEEWIYALQSLCSGSKSPR--YPHLGGQVDIGQLKTHNALAVFVCILV 1216
            ||:|:.|||||..|..||.||:..|::||..|.:..  :..|...||:..|..|::||.||.||:
Human  1072 NDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILI 1136

  Fly  1217 ARHCFSLADFVSKFALPTLARSVSAGGAELSVDAEAGARLTCHLVLKLFKTLEIPQPGMYSVSTS 1281
            ||.|..|.|.:...|:|:|..:..:     ..|:|.||||||.::|.||||.::
Human  1137 ARQCLLLEDLIRCAAIPSLLNAACS-----EQDSEPGARLTCRILLHLFKTPQL----------- 1185

  Fly  1282 PNPLHAVGN--DFSIRLSCDRHLLVGAHKTIPIAAVLAVLKAILIVVDNAALKTPLASGSG-TSS 1343
             ||..:.||  ...||.|||||||..:...|...||.|||||:.::.| |.||     ||| |.:
Human  1186 -NPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGD-AELK-----GSGFTVT 1243

  Fly  1344 GGL-------GGAFGSGKRSGFNTPVHPGSTPKSNEQRPADLSQILGTSDLQLGSSLTSEPEALQ 1401
            ||.       ||....|:|.|                                |.:::
Human  1244 GGTEELPEEEGGGGSGGRRQG--------------------------------GRNIS------- 1269

  Fly  1402 QPSVGGMEQISLLEFAQAVLKQICAQEHVLERCLKNAEQLCDMIIDEMLTAKQAQRVLHMICYPE 1466
                  :|..||..:|:.||:.||.||.|.|||||:..:..:.:.|.:|::.||||::.:||||.
Human  1270 ------VETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPH 1328

  Fly  1467 PEF-NIISELDQRSMIVRILENLGQWTLRISWLDLQLMYRQSLSNNAELNVWLDTVARAAIDVFH 1530
            ... |...|..||..|.|||:||.|||:|.|.|:||||.:|:.:|  |:|..|:.:|:|.|:||.
Human  1329 RLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNN--EMNSLLENIAKATIEVFQ 1391

  Fly  1531 MEEVV----------LPGAVKATHKP------KPSTWLVAPLIAKLTPAVQGRILRVAGQVLESM 1579
            .....          :|.:.|.  ||      :...||||||||||..:|||.:|:.||:.||..
Human  1392 QSAETGSSSGSTASNMPSSSKT--KPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKG 1454

  Fly  1580 NYFSKVSKSDCNSSGSGDEREKSNSCHSSNSYGLGGVPARNKKMPLDYQPFLGLILTCLKGQDEY 1644
            .:.           ||...:|:......|.|.             |..||||.|:|||||||||.
Human  1455 QHL-----------GSSSRKERDRQKQKSMSL-------------LSQQPFLSLVLTCLKGQDEQ 1495

  Fly  1645 KENLLVSLYAQLSQCLQSFAELDTIGGIDEPQAREEILDALQLRFSLVGGMFEAIQKNSTPTTDW 1709
            :|.||.|||:|:.|.:.::.: |..  :|:.:.::.:.:||:||.:||||||:.:|:::..||:|
Human  1496 REGLLTSLYSQVHQIVNNWRD-DQY--LDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEW 1557

  Fly  1710 AILLAQLVCQGVVDLSCNRELFTTVVDMLATLVHSTLVSD-----------DERHYMNLMKKLKK 1763
            |:||.:::..|.||:..|.||||||:|||:.|::.||.:|           ::|.||||.|||:|
Human  1558 AMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQK 1622

  Fly  1764 EIGEKNNASIRVIRQLLPLYKQPTEVIACEHSG--MDTKGNKICDID----KKQLRISDKQRISV 1822
            |:||:.:.|:..:||||||.||..:||.||..|  :|||||||...|    |:.|::|.||:||.
Human  1623 ELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISP 1687

  Fly  1823 WDILEGHKNPAPLSWVWFGAVKLERKPLTYEEAHRNLKYHTHSLVKPSSYYYEPLPLPPEDIEPV 1887
            ||:.||.|..|||||.|||.|:::|:....||..|.|.||||...:|.:||.|||||||||.||
Human  1688 WDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEP- 1751

  Fly  1888 PEKICIKDEMKADTPSSVDQSPSAVVGGTGRGRGKGTTTRKRKPKNPKTPPVVNTQQQQPQLAQQ 1952
            |....::.|.||..|...|: |.|....|...:.|.|..:||.....||
Human  1752 PAPTLLEPEKKAPEPPKTDK-PGAAPPSTEERKKKSTKGKKRSQPATKT---------------- 1799

  Fly  1953 PQQPQNVQQQQLQQQQQQQQHMQQQHMQQQQMQPNQMGQMPMNMPMNMQQFAPNPNNMMQQNAML 2017
                                       :...|.|.:.|...:.:|.::... |||.::...|
Human  1800 ---------------------------EDYGMGPGRSGPYGVTVPPDLLHH-PNPGSITHLN--- 1833

  Fly  2018 QQQQQQQMQQMGNNPMQQQLNVGGGNGQPNPQMNFMQQ----GPGGGGAGPQGMPG----QQQQW 2074
                 .:...:|.....|.|..||....|...:....|    .|...|..|..|.|    :...:
Human  1834 -----YRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSY 1893

  Fly  2075 HNAP-QQQQPPQPYHNQYAPHQQNMQSNRIERPPLNANSKQALSQMLRQRQPFQQQA---QQGPG 2135
            ..:. :||||..|                         ..|.|.|.|:|.|....|:   |..|.
Human  1894 KTSVYRQQQPAVP-------------------------QGQRLRQQLQQSQGMLGQSSVHQMTPS 1933

  Fly  2136 GGFNPMQQQ---PQAS----QQQPGPQQQMNP---NQMRQQQMNPQQNPQSVAAFNAMQQQPQQN 2190
            ..:.....|   |..|    ||..||...|.|   :....|..:|..||..|.....:||:|...
Human  1934 SSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGY 1998

  Fly  2191 AQQQQMNPNQQQQQQFMRGGNMRPGMAPNQMNQMNMGGQGMSQNPMMQQQIPQN--MVGMVNPNA 2253
            ..||                      ||..       |.|::.......|..|.  |:..:.|.:
Human  1999 VHQQ----------------------APTY-------GHGLTSTQRFSHQTLQQTPMISTMTPMS 2034

  Fly  2254 NQMMQSGGAQGGNGVGVGVGVGVGGAGNNPNMGMGGMPQQGMIQQQPQQQPQQQVQFQNFQNQYQ 2318
            .|               ||..||......|.            |||.|||.|||      |.|.|
Human  2035 AQ---------------GVQAGVRSTAILPE------------QQQQQQQQQQQ------QQQQQ 2066

  Fly  2319 QQQQQGMQQQGGGAGVGVGVGMAPNQQQQQQANMMGNFNPQMQQGNRNNPDFMAAAAVAQQQQQQ 2383
            |||||..|||           ....||||||                         .:.||||||
Human  2067 QQQQQQQQQQ-----------YHIRQQQQQQ-------------------------ILRQQQQQQ 2095

  Fly  2384 QQQQRVVPGGMMAGNRNQYMNQAPNVTMSTMMGPGPGGVVGQVPPYARQQSAGGGKPGVLNTQQQ 2448
            ||||:                                                         |||
Human  2096 QQQQQ---------------------------------------------------------QQQ 2103

  Fly  2449 FQQQQQQQQQLRHQMMQLQGMGGGAGGGMGAGPQQGGGAVGGGAGGGMVPQ------------QQ 2501
            .|||||||||.:||..|.|         ..|.||               ||            ||
Human  2104 QQQQQQQQQQQQHQQQQQQ---------QAAPPQ---------------PQPQSQPQFQRQGLQQ 2144

  Fly  2502 SMNQQQTPNLVAQLQRQNMMGQQQYQP 2528
            :..||||..||.|||:|  :...|.||
Human  2145 TQQQQQTAALVRQLQQQ--LSNTQPQP 2169

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
kto	NP_524786.1	Med12	99..157	CDD:286572	27/57 (47%)
kto	NP_524786.1	Med12-LCEWAV	390..856	CDD:288959	184/519 (35%)
MED12	NP_005111.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	12..35		18/22 (82%)
		Med12	103..161	CDD:286572	27/57 (47%)
		Med12-LCEWAV	286..757	CDD:288959	184/519 (35%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	323..344		4/20 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	627..669		11/64 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	690..717		5/26 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1241..1266		8/56 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1394..1415		2/22 (9%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1450..1474		6/34 (18%)
		Interaction with CTNNB1 and GLI3. /evidence=ECO:0000269\|PubMed:17000779	1616..2051		156/569 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1738..1829		34/136 (25%)
		Med12-PQL	1820..2019	CDD:288958	53/261 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1919..1938		4/18 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1967..1989		5/21 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2115..2149		11/57 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2158..2177		6/14 (43%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165141342
Domainoid	1	1.000	714	1.000	Domainoid score	I117
eggNOG	1	0.900	-	-		E1_KOG3598
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1250	1.000	Inparanoid score	I178
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45902
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0004348
OrthoInspector	1	1.000	-	-		otm42165
orthoMCL	1	0.900	-	-		OOG6_105637
Panther	1	1.100	-	-	LDO	PTHR46007
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X4125
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	13	12.760