DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo2 and Robo4

DIOPT Version :9

Sequence 1:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster
Sequence 2:	XP_006510707.1	Gene:	Robo4 / 74144	MGIID:	1921394	Length:	1074	Species:	Mus musculus

Alignment Length:	1004	Identity:	226/1004 - (22%)
Similarity:	365/1004 - (36%)	Gaps:	245/1004 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    68 FPLLLLLAGLNGLTQVGALKGENPRIIEHPMDTTVPKNDPFTFNCQAEGNPTPTIQWFKDGRELK 132
            :||.|||..:.|    |......|:|:.||.|..:..:.|....|::.|.|.|||:|..:|:.|.
Mouse    23 WPLPLLLLFIMG----GEALDSPPQILVHPQDQLLQGSGPAKMRCRSSGQPPPTIRWLLNGQPLS 83

  Fly   133 TDTGSHRIMLPAGGLFFLK-VIHSRRESD-------AGTYWCEAKNEFGVARSRNATLQVAFLRD 189
            ..|.....:||.|.|...: .:..|.:.|       .|.|.|||.|..|.|.||.|.|.||.|::
Mouse    84 MATPDLHYLLPDGTLLLHRPSVQGRPQDDQNILSAILGVYTCEASNRLGTAVSRGARLSVAVLQE 148

  Fly   190 EFRLEPANTRVAQGEVALMECGAPRGSPEPQISWRKNGQTLNLVGNKRIRIVDGGNLAIQEARQS 254
            :|:::|.:|....||..::|||.|.|.|:|.:||.|:|:.|.|...:  |.|.|.:|.:..|.::
Mouse   149 DFQIQPRDTVAVVGESLVLECGPPWGYPKPSVSWWKDGKPLVLQPGR--RTVSGDSLMVSRAEKN 211

  Fly   255 DDGRYQCVVKNVVGTRESATAFLKVH-----------------------VRPFLIRGPQNQTAVV 296
            |.|.|.|:..|..|.|||..|.:.:.                       :.|..::||:     .
Mouse   212 DSGTYMCMATNNAGQRESRAARVSIQESQDHKEHLELLAVRIQLENVTLLNPEPVKGPK-----P 271

  Fly   297 GSSVVFQCRIGGDPLP----DVLWR--RTASGGNMPLRRVHVLEDRSLKLDDVTLEDMGEYTCEA 355
            |.||....::.|...|    ..|:|  |:......|...|.:...:|.||..:......|:....
Mouse   272 GPSVWLSWKVSGPAAPAESYTALFRTQRSPRDQGSPWTEVLLRGLQSAKLGGLHWGQDYEFKVRP 336

  Fly   356 DNAVGGITATGILTVHAPPKF-VIRPKNQLVEIGDEVLFECQA-------NGHPRPTLYWSVEGN 412
            .:.......:.:|.:..|.:. ...|:...:..|:..:|...|       ||..|....||: ||
Mouse   337 SSGRARGPDSNVLLLRLPEQVPSAPPQGVTLRSGNGSVFVSWAPPPAESHNGVIRGYQVWSL-GN 400

  Fly   413 SSLLLPGY----RDGRMEVTLTPEGRSVLSIARFAREDSGKVVTCNALNAVGSVSSRTVVSVDTQ 473
            :||....:    ...::|:.....|...:.:|......:|::.|...| .:|...:.::.|.
Mouse   401 ASLPAANWTVVGEQTQLEIATRLPGSYCVQVAAVTGAGAGELSTPVCL-LLGEGKALSISST--- 461

  Fly   474 FELPPPIIEQGPVNQTL-------------PVKSIVVLPC-----------RTLGTPVPQVSWYL 514
               |||.  :..|:|.|             |:...:.||.           ::...|...|.|.|
Mouse   462 ---PPPC--RPSVSQFLSYIFSSSLSCLLVPLTVPLALPLSSTSQTEQAMEQSARDPRKHVPWTL 521

  Fly   515 DGIPIDVQEHERRNLSDAGAL--------TISDLQR-----HEDEGLY----------------- 549
            :.:...::..|  .::.:..|        |:...:|     |...|||
Mouse   522 EQLRATLRRPE--VIASSAVLLWLLLLGITVCIYRRRKAGVHLGPGLYRYTSEDAILKHRMDHSD 584

  Fly   550 ----------TCVASNRNGKSSWSGYLRLD--------------TPTNPNIKFFRAPELSTYPG- 589
                      |..:.:.:..||.|..|.||              .|.:|.:...  |:.||:.|
Mouse   585 SPWLADTWRSTSGSRDLSSSSSLSSRLGLDPRDPLEGRRSLISWDPRSPGVPLL--PDTSTFYGS 647

  Fly   590 --------PPGKPQMVEKGEN-------SVTLSWTRSNKVGGSSLVGYVIEMFGKNETDGWVAVG 639
                    ||.:|........       ..:..|..|:.:  .|..|..........|:.|.|..
Mouse   648 LIAEQPSSPPVRPSPKTPAARRFPSKLAGTSSPWASSDSL--CSRRGLCSPRMSLTPTEAWKAKK 710

  Fly   640 TR---VQNTTFTQTGLLPGVNYFFLIRAENSHGLS-LPSPMSEPITVGTRYFNSGLDLSEARASL 700
            .:   ..|::....|..|...:.:.:.:..|..|| .|.|            ||| ...||..::
Mouse   711 KQELHQANSSPLLRGSHPMEIWAWELGSRASKNLSQSPGP------------NSG-SPGEAPRAV 762

  Fly   701 LSGDVV--EL-SNASVVDSTSMKLTWQIING--------KYVEGFYVYARQLP--NPIVNNPAPV 752
            :|...|  :| .|:|.:.|..:..|...:.|        :.||..     |.|  :|:...|..|
Mouse   763 VSWRAVGPQLHRNSSELASRPLPPTPLSLRGASSHDPQSQCVEKL-----QAPSSDPLPAAPLSV 822

  Fly   753 TSNTNP------LLGSTSTSAS--ASASASAL--------ISTKPNIAAAGK-RDGETNQSGGGA 800
            .:::.|      .|...|.|:|  :|:|.|:|        :.|...:|...: .|||...:...:
Mouse   823 LNSSRPSSPQASFLSCPSPSSSNLSSSSLSSLEEEEDQDSVLTPEEVALCLELSDGEETPTNSVS 887

  Fly   801 PTPL----NTKYRMLTILN----------GGGASSCTITGLVQYTLYEFFIVPFYKSVEGKPSNS 851
            |.|.    .|.|..::|..          |||..|     .|...||.....|.....||..:|.
Mouse   888 PMPRAPSPPTTYGYISIPTCSGLADMGRAGGGVGS-----EVGNLLYPPRPCPTPTPSEGSLANG 947

  Fly   852 RIARTLEDVPSEAPYGMEALLLNSSAVFL 880
            ..:.:.::|||    ...:|:.:|...||
Mouse   948 WGSASEDNVPS----ARASLVSSSDGSFL 972

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	34/102 (33%)
		I-set	91..184	CDD:254352	34/100 (34%)
		I-set	190..279	CDD:254352	33/88 (38%)
		Ig2_Robo	193..279	CDD:143201	32/85 (38%)
		I-set	283..370	CDD:254352	18/92 (20%)
		Ig	300..370	CDD:299845	13/75 (17%)
		Ig	388..>457	CDD:299845	17/79 (22%)
		I-set	479..561	CDD:254352	20/145 (14%)
		Ig	496..561	CDD:143165	15/115 (13%)
		FN3	588..681	CDD:238020	19/112 (17%)
		FN3	<817..856	CDD:238020	10/38 (26%)
		fn3	864..952	CDD:278470	4/17 (24%)
Robo4	XP_006510707.1	Ig	41..144	CDD:386229	34/102 (33%)
		Ig	152..236	CDD:386229	32/85 (38%)
		FN3	361..446	CDD:238020	18/85 (21%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	0	0.000