DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo2 and ROBO3

DIOPT Version :9

Sequence 1:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster
Sequence 2:	NP_071765.2	Gene:	ROBO3 / 64221	HGNCID:	13433	Length:	1386	Species:	Homo sapiens

Alignment Length:	1567	Identity:	437/1567 - (27%)
Similarity:	651/1567 - (41%)	Gaps:	396/1567 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly    77 LNGLTQVGALKGENPRIIEHPMDTTVPKNDPFTFNCQAEGNPTPTIQWFKDGRELKT---DTGSH 138
            ||| ::||. :...|||:|.|.|..|.:.:|.|..|:|||.|.|.|:|:|:|..:.|   |..:|
Human    52 LNG-SRVGP-EDAMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATVREDPRAH 114

  Fly   139 RIMLPAGGLFFLKVIHSRR-ESDAGTYWCEAKNEFGVARSRNATLQVAFLRDEFRLEPANTRVAQ 202
            |::||:|.|||.:::|.|| ..|.|.|.|.|:|..|.|.||||:|:||.|||:||..|.|..||.
Human   115 RLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAV 179

  Fly   203 GEVALMECGAPRGSPEPQISWRKNGQTLNLVGNKRIRI-VDGGNLAIQEARQSDDGRYQCVVKNV 266
            ||.|::||..|||.|||.:||||:|..|.   .:..|| :.||.|.:....:||.|.|.||..|:
Human   180 GEPAVLECVPPRGHPEPSVSWRKDGARLK---EEEGRITIRGGKLMMSHTLKSDAGMYVCVASNM 241

  Fly   267 VGTRESATAFLKVHVRPFLIRGPQNQTAVVGSSVVFQCRIGGDPLPDVLWRRTASGGNMPLRRVH 331
            .|.||||.|.:.|..||..:|.|.||..:..:.|.|.|.:.|||.|.:.||:  ..|.:|..|..
Human   242 AGERESAAAEVMVLERPSFLRRPVNQVVLADAPVTFLCEVKGDPPPRLRWRK--EDGELPTGRYE 304

  Fly   332 VLEDRSLKLDDVTLEDMGEYTCEADNAVGGITATGILTVHAPPKFVIRPKNQLVEIGDEVLFECQ 396
            :..|.||.:..|:.||.|.|||.|:|:||...|:|.|:||.||:.|.:|::|:...|:.|.|:|:
Human   305 IRSDHSLWIGHVSAEDEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCE 369

  Fly   397 ANGHPRPTLYWSVEGNSSLLLPGYR---DGRMEVTLTPEGRSVLSIARFAREDSGKVVTCNALNA 458
            ..|:|.|.::|..||:..||.|...   .||..|  :|.|:  |:|....|.|:|..| |.|::.
Human   370 TKGNPPPAIFWQKEGSQVLLFPSQSLQPTGRFSV--SPRGQ--LNITAVQRGDAGYYV-CQAVSV 429

  Fly   459 VGSVSSRTVVSV-DTQFELPPPIIEQGPVNQTLPVKSIVVLPCRTLGTPVPQVSWYLDGIPIDVQ 522
            .||:.::.::.: ....:..||:|.|||.||||.:.|.|.||||..|.|.|.|.|..||..:...
Human   430 AGSILAKALLEIKGASLDGLPPVILQGPANQTLVLGSSVWLPCRVTGNPQPSVRWKKDGQWLQGD 494

  Fly   523 EHERRNLSDAGALTISDLQRHEDEGLYTCVASNRNGKSSWSGYLRL--------DTPTNPNIKFF 579
            :.:.:.::: |.|.|:::| ..|.|.|:|||.:..|:::|||:|::        |.||.|
Human   495 DLQFKTMAN-GTLYIANVQ-EMDMGFYSCVAKSSTGEATWSGWLKMREDWGVSPDPPTEP----- 552

  Fly   580 RAPELSTYPGPPGKPQMVEKGENSVTLSWTRSNKVGGSSLVGYVIEMFGKNETDGWVAVGTRVQN 644
                 |:.||.|.:|.:.|..:||:||:| :.|...|:::..||||.|.....:.|..|...||.
Human   553 -----SSPPGAPSQPVVTEITKNSITLTW-KPNPQTGAAVTSYVIEAFSPAAGNTWRTVADGVQL 611

  Fly   645 TTFTQTGLLPGVNYFFLIRAENSHGLSLPSPMSEPITVGTRYFNSGLDLSEAR--------ASLL 701
            .|.|.:||.|...|.||:||..:.|||.|||:|||:..        .|.|.:|        ...|
Human   612 ETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPVRT--------QDSSPSRPVEDPWRGQQGL 668

  Fly   702 SGDVVELSNASVVDSTSMKLTWQIING--KYVEGFYVYARQLPNPIVNNPAPVTSNTNPLLGS-T 763
            :...|.|....|:...:::::| .::|  :.|:||.|..|.               ..|..|| |
Human   669 AEVAVRLQEPIVLGPRTLQVSW-TVDGPVQLVQGFRVSWRV---------------AGPEGGSWT 717

  Fly   764 STSASASASASALISTKP-------NIAAAGKRDGETNQSGGGAPTPLNTKYRMLTILNGGGASS 821
            .....:.:..|.::...|       .:.|.|:                          .|.||.|
Human   718 MLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQ--------------------------EGLGAES 756

  Fly   822 CTITGLVQYTLYEFFIVPFYKSVEGKPSNSRIARTLEDVPSEAPYGMEALL--LNSSAVFLKWKA 884
            .::|                :|:.            |:.||..|.|:...|  ..:|::.:.|:.
Human   757 LSVT----------------RSIP------------EEAPSGPPQGVAVALGGDGNSSITVSWEP 793

  Fly   885 PELKDRHGVLLNYHVIVRGIDTAHNFSRILTNVTIDAASPTLVLANLTEGVMYTVGVAAGNNAGV 949
            |....::||:..|.:...|     |.||...|.:....:.:.:|..|..|::|...|||..:|||
Human   794 PLPSQQNGVITEYQIWCLG-----NESRFHLNRSAAGWARSAMLRGLVPGLLYRTLVAAATSAGV 853

  Fly   950 GPYCVPATLRLDPITKRLDPFINQRYPINQDHVNDVLTQPWFIILLGAILAVLMLSFGAMVFVKR 1014
            |....|..::| |....|:|.:.....: ...:..||.:|.|:...||....|:|...|.::.:|
Human   854 GVPSAPVLVQL-PSPPDLEPGLEVGAGL-AVRLARVLREPAFLAGSGAACGALLLGLCAALYWRR 916

  Fly  1015 KHMMMKQSALNTMRGNHTSDVLKMPSLSARNGNGY-----------------WLDSSTGGMVW-R 1061
            |    ::..|:    ::|:.....|::|..:..|.                 ||..|     | .
Human   917 K----QRKELS----HYTASFAYTPAVSFPHSEGLSGASSRPPMGLGPAPYSWLADS-----WPH 968

  Fly  1062 PS-------PGGDSLEMQKD-------------HIADYAPVCGAPG--------SPAG------- 1091
            ||       |.|.......|             ::|..|....|||        .|||
Human   969 PSRSPSAQEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGTAAPGEGPVYSTIDPAGEELQTFH 1033

  Fly  1092 GGTSSGGSGGAG--------------SGASGGDDIHGGHGSERNQQRYVGEYSNIPT-------- 1134
            ||.....||..|              |||.||            :.:.:|:...:|:
Human  1034 GGFPQHPSGDLGPWSQYAPPEWSQGDSGAKGG------------KVKLLGKPVQMPSLNWPEALP 1086

  Fly  1135 ---DYAEVSSFGKAPSEYGRHGNASPAPYA-----TSSILSPHQQ-------QQQQQPRYQQRPV 1184
               ...|:|.. :.|.| ...|::.|..:.     .|.:..|...       .:::.|    .|.
Human  1087 PPPPSCELSCL-EGPEE-ELEGSSEPEEWCPPMPERSHLTEPSSSGGCLVTPSRRETP----SPT 1145

  Fly  1185 PGYGLQR-----------PMHPHYQQQQHQQQQAQQTHQQHQALQQHQQLP-----PSNIYQQMS 1233
            |.||.|.           |..|......||..               :::|     |.::.|.|.
Human  1146 PSYGQQSTATLTPSPPDPPQPPTDMPHLHQMP---------------RRVPLGPSSPLSVSQPML 1195

  Fly  1234 TTSEIYPT--NTGPSRSVYSEQYYYPKDKQRHIHITENKLSNCHTYEAAPG------AKQSSPIS 1290
            ...|..|.  ..||:.|.               |::.:...:  |..:|||      .:.:.|:.
Human  1196 GIREARPAGLGAGPAASP---------------HLSPSPAPS--TASSAPGRTWQGNGEMTPPLQ 1243

  Fly  1291 SQFASVRRQQLPPNCSIGRESARFKVLNTDQGKNQQNLLDLDGSSMCYNGLADSGCGGSPSPMAM 1355
            ...|..|::  |......||::.                                 |..|.|...
Human  1244 GPRARFRKK--PKALPYRRENSP---------------------------------GDLPPPPLP 1273

  Fly  1356 LMSHEDEHALYHTADGDLDDMERLYVKVDEQQPPQQQQQLIPLVPQ---HPAEGHLQSWRNQSTR 1417
            ....|...||...|.|.:..:||      |:...::..|.:||..|   ||.|   ::|...|..
Human  1274 PPEEEASWALELRAAGSMSSLER------ERSGERKAVQAVPLAAQRVLHPDE---EAWLPYSRP 1329

  Fly  1418 SSRKNGQ---ECIKEPSELIYAPGSVASERSLLSNSGSGTSSQPAGHNVXPLLPGSSLGQSQSKS 1479
            |....||   .|            |.|.     |||..|:||......     ||.|..:|||:|
Human  1330 SFLSRGQGTSTC------------STAG-----SNSSRGSSSSRGSRG-----PGRSRSRSQSRS 1372

  Fly  1480 QSQSQSQ 1486
            |||...|
Human  1373 QSQRPGQ 1379

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	45/98 (46%)
		I-set	91..184	CDD:254352	45/96 (47%)
		I-set	190..279	CDD:254352	41/89 (46%)
		Ig2_Robo	193..279	CDD:143201	39/86 (45%)
		I-set	283..370	CDD:254352	34/86 (40%)
		Ig	300..370	CDD:299845	29/69 (42%)
		Ig	388..>457	CDD:299845	26/71 (37%)
		I-set	479..561	CDD:254352	33/81 (41%)
		Ig	496..561	CDD:143165	23/64 (36%)
		FN3	588..681	CDD:238020	40/92 (43%)
		FN3	<817..856	CDD:238020	5/38 (13%)
		fn3	864..952	CDD:278470	25/89 (28%)
ROBO3	NP_071765.2	Ig	64..162	CDD:299845	45/97 (46%)
		I-set	64..161	CDD:254352	45/96 (47%)
		I-set	168..249	CDD:254352	39/83 (47%)
		Ig	170..254	CDD:299845	39/86 (45%)
		I-set	258..343	CDD:254352	34/86 (40%)
		Ig	275..343	CDD:299845	29/69 (42%)
		I-set	347..441	CDD:254352	32/98 (33%)
		Ig	361..450	CDD:299845	28/93 (30%)
		I-set	451..531	CDD:254352	33/81 (41%)
		IGc2	465..528	CDD:197706	23/64 (36%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	541..563		8/31 (26%)
		FN3	556..649	CDD:238020	40/93 (43%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	639..662		9/30 (30%)
		FN3	680..760	CDD:238020	20/121 (17%)
		FN3	770..863	CDD:238020	26/97 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	965..989		6/28 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1028..1310		62/372 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1327..1386		24/75 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6690
eggNOG	1	0.900	-	-		E1_KOG4222
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D173520at2759
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	9	8.680