DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo2 and ROBO4

DIOPT Version :9

Sequence 1:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster
Sequence 2:	NP_061928.4	Gene:	ROBO4 / 54538	HGNCID:	17985	Length:	1007	Species:	Homo sapiens

Alignment Length:	1140	Identity:	246/1140 - (21%)
Similarity:	370/1140 - (32%)	Gaps:	392/1140 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly    65 RAVFPLLLLLAGLNGLTQVGALKGENPRIIEHPMDTTVPKNDPFTFNCQAEGNPTPTIQWFKDGR 129
            |...||||||. :.|:.|     ...|:|:.||.|.......|...:|||.|.|.|||:|..:|:
Human    12 RGSLPLLLLLI-MGGMAQ-----DSPPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQ 70

  Fly   130 ELKTDTGSHRIMLPAGGLFFLKV-----IHSRR--ESDAGTYWCEAKNEFGVARSRNATLQVAFL 187
            .|.........:||.|.|..|:.     .|..:  .:|.|.|.|||.|..|.|.||.|.|.||.|
Human    71 PLSMVPPDPHHLLPDGTLLLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRGARLSVAVL 135

  Fly   188 RDEFRLEPANTRVAQGEVALMECGAPRGSPEPQISWRKNGQTLNLVGNKRIRIVDGGNLAIQEAR 252
            |::|:::|.:.....||...:|||.|.|.|||.:||.|:|:.|.|...:  ..|.||:|.:..|.
Human   136 REDFQIQPRDMVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGR--HTVSGGSLLMARAE 198

  Fly   253 QSDDGRYQCVVKNVVGTRESATAFLKVHVRPFLIRGPQNQTAVVGSSVVFQCRIGGDPLPDVLWR 317
            :||:|.|.||..|..|.|||..|.:.       |:.||:.|               :|
Human   199 KSDEGTYMCVATNSAGHRESRAARVS-------IQEPQDYT---------------EP------- 234

  Fly   318 RTASGGNMPLRRVHVLEDRSLKLDDVTLEDMGEYTCEADNAVGGITATGILTVHAPPKFVIRPKN 382
                        |.:|..| ::|::|||       ...|.|.|                   ||
Human   235 ------------VELLAVR-IQLENVTL-------LNPDPAEG-------------------PK- 259

  Fly   383 QLVEIGDEVLFECQANGHPRPTLY--WSVEGNSSLLLPGYRDGRMEVTLTPEGRSVLSIARFARE 445
                              |||.::  |.|.|.::
Human   260 ------------------PRPAVWLSWKVSGPAA------------------------------- 275

  Fly   446 DSGKVVTCNALNAVGSVSSRTVVSVDTQFELPPPIIEQGPVNQTLPVKSIVVLPCRTLGTPVPQV 510
                                                         |.:|...| .||...|..|.
Human   276 ---------------------------------------------PAQSYTAL-FRTQTAPGGQG 294

  Fly   511 SWYLDGIPIDVQEHERRNLSDAGALTISDLQRHEDEGLYTCVASNR-NGKSSWSGYLRLDTPTNP 574
            :.:.:.:....|..|           :..|...:|.......:|.| .|..|
Human   295 APWAEELLAGWQSAE-----------LGGLHWGQDYEFKVRPSSGRARGPDS------------- 335

  Fly   575 NIKFFRAPELSTYPGPPGKPQMVEKGENSVTLSWTRSNKVGGSSLV-GYVIEMFGKNETD--GWV 636
            |:...|.||  ..|..|.:...::.|..:|.:||........:.:: ||.:...|.....  .|.
Human   336 NVLLLRLPE--KVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSLPPANWT 398

  Fly   637 AVGTRVQNTTFTQTGLLPGVNYFFLIRAENSHGLSLPS-PM------------SEPITVGTRYFN 688
            .||.:.|....|.   :|| :|...:.|....|...|| |:            .||...|.
Human   399 VVGEQTQLEIATH---MPG-SYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGP---- 455

  Fly   689 SGLDLSEARASLLSGDVVELSNASVVDSTSMKLTWQIINGKYV-------------EGFYVYARQ 740
              ..|.:.||:|...:|:.....::         |.::.|..|             .|.|.|..:
Human   456 --WTLEQLRATLKRPEVIATCGVAL---------WLLLLGTAVCIHRRRRARVHLGPGLYRYTSE 509

  Fly   741 LPNPIVNNPAPVTSNTNPLLGST--STSASASASASALISTKPNIAAAGKRDGETNQSGGGAPTP 803
              :.|:.:  .:..:.:..|..|  |||.|...|:|:.:|::               .|..|..|
Human   510 --DAILKH--RMDHSDSQWLADTWRSTSGSRDLSSSSSLSSR---------------LGADARDP 555

  Fly   804 LNTKYRMLT-ILNGGGASSCTITGLVQYTLYEFFIVPFYKSVEGKPSNS-----RIARTLEDVPS 862
            |:.:..:|: .....|......|.    |.|...|.....|...:||..     |:...|..:.|
Human   556 LDCRRSLLSWDSRSPGVPLLPDTS----TFYGSLIAELPSSTPARPSPQVPAVRRLPPQLAQLSS 616

  Fly   863 EAPYGMEALL----LNSSAVFL----KWKAPELKD-RHGVLLNYHVIVRGIDTAHNFSRILTNVT 918
            ... ..::|.    |:|..:.|    .|||.:.:: :|.   |...::||   :|       ::.
Human   617 PCS-SSDSLCSRRGLSSPRLSLAPAEAWKAKKKQELQHA---NSSPLLRG---SH-------SLE 667

  Fly   919 IDAASPTLVLANLTEGVMYTVGVAAGNNAGVGPYCVPATL----RLDP---------ITKRLDP- 969
            :.|..               :|.....|....|..||..|    .|.|         :|:.|.|
Human   668 LRACE---------------LGNRGSKNLSQSPGAVPQALVAWRALGPKLLSSSNELVTRHLPPA 717

  Fly   970 --FINQRYPINQDHVNDVLT--QPWFIILLGAILAVL-----------MLSFGAMVFVKRKHMMM 1019
              |.::..|.........:.  .|..|:|..|.:.:|           .|| |......|    :
Human   718 PLFPHETPPTQSQQTQPPVAPQAPSSILLPAAPIPILSPCSPPSPQASSLS-GPSPASSR----L 777

  Fly  1020 KQSALNTMRGNHTSDVLKMPSLSARNGNGYWLDSSTGGMVWRPSPGGDSLEMQKDHIADYAPVCG 1084
            ..|:|::: |.....||....::      ..|:.|.|....|.|               .:|:..
Human   778 SSSSLSSL-GEDQDSVLTPEEVA------LCLELSEGEETPRNS---------------VSPMPR 820

  Fly  1085 APGSPAGGG----------TSSGGSGGAGSGASGG 1109
            ||..|...|          |..|.:|| |.|..||
Human   821 APSPPTTYGYISVPTASEFTDMGRTGG-GVGPKGG 854

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	36/101 (36%)
		I-set	91..184	CDD:254352	36/99 (36%)
		I-set	190..279	CDD:254352	35/88 (40%)
		Ig2_Robo	193..279	CDD:143201	34/85 (40%)
		I-set	283..370	CDD:254352	15/86 (17%)
		Ig	300..370	CDD:299845	11/69 (16%)
		Ig	388..>457	CDD:299845	6/70 (9%)
		I-set	479..561	CDD:254352	14/82 (17%)
		Ig	496..561	CDD:143165	12/65 (18%)
		FN3	588..681	CDD:238020	24/108 (22%)
		FN3	<817..856	CDD:238020	9/43 (21%)
		fn3	864..952	CDD:278470	15/96 (16%)
ROBO4	NP_061928.4	Ig	31..133	CDD:299845	36/101 (36%)
		I-set	32..132	CDD:254352	36/99 (36%)
		I-set	139..220	CDD:254352	34/82 (41%)
		Ig2_Robo	141..225	CDD:143201	34/92 (37%)
		fn3	350..432	CDD:278470	18/85 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	531..551		8/34 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	713..793		16/85 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	806..826		6/34 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	971..1007
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	81	1.000	Domainoid score	I8493
eggNOG	1	0.900	-	-		E1_KOG4222
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	3	2.860