DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo2 and plum

DIOPT Version :9

Sequence 1:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster
Sequence 2:	NP_001163744.1	Gene:	plum / 43197	FlyBaseID:	FBgn0039431	Length:	1298	Species:	Drosophila melanogaster

Alignment Length:	1377	Identity:	263/1377 - (19%)
Similarity:	439/1377 - (31%)	Gaps:	478/1377 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly   220 QISWRKNGQTLNLVGNKRIRIVDGGNLAIQEARQSDDGRYQCVVKNVVGTRESATAFLKVHVR-P 283
            |:|:|||.          ..|.|.|.|.: ...:...|.|.|.::    ..|||....::.|. |
  Fly   107 QVSYRKND----------FFIYDNGTLRL-PTNEKASGEYYCKIE----WDESAVISDRITVEYP 156

  Fly   284 FLIR---GPQ---NQTAVVGSSVVFQCRIGGDPLPDVLW---RRTASGGNMPLRRVHVLEDRSLK 339
            .|.|   |.|   |.:|:....:|..|.....|.....|   ..:.:..|..|    :|.:.:|.
  Fly   157 VLKRLFPGQQQAANISALENKPLVLSCPFLSVPAARFSWYFGNDSLTNDNSAL----ILTNGTLI 217

  Fly   340 LDDVTLEDMGEYTCEADNAVGGIT-ATGILTVHAPPK-FVIRPKNQLVEIGDEVLFECQANGHPR 402
            |..:.||..|:|.|.|.|.....| ...|..:...|| ....|||: ..|.:..:.|.|
  Fly   218 LRHLRLEQAGKYKCVAQNTFASKTHRQFIWQLQVEPKDATFYPKNE-AGITETAVSEAQ------ 275

  Fly   403 PTLYWSVEGNSSLLLPGYRDGRMEVTLTPEGRSVL----SIARFA------REDSGKVVTCNALN 457
                         |||.:::..:.|   |.|.|||    ::|.|.      :..:.|:|..:..:
  Fly   276 -------------LLPAFQNATVFV---PAGGSVLLQCHAVADFVPIVWYLQPPNNKMVRLSNNS 324

  Fly   458 AVGSVSSRT----------VVSVDTQ-FEL----PPPIIEQGPVNQTLPVKSIVV-----LPCRT 502
            ...|:::.:          :..::|| |::    ||.|:.:      |||:.:.:     |.||.
  Fly   325 VAYSITNASIARHEGRYSCITPLETQNFQVIVTTPPRIVSR------LPVEVVYIGLSRTLTCRA 383

  Fly   503 LGTPVPQVSWYLDGIPIDVQEHERRNLSDAGALTISDLQRH----EDEGLYTCVASNRNGKSSWS 563
            .|.|.|.::||.:|:.:        |.|....::.::|..|    ::||:|.|||.|..|:.|.:
  Fly   384 EGHPEPSITWYHNGVHL--------NSSYTRYISGNELHVHSFDSKEEGIYQCVARNVAGEDSAT 440

  Fly   564 GYLRLD---TPTNP--NIKFFRAPELSTYPGPPGKPQMVEKGENSVTLSWTRSNKVGGSSLVGYV 623
            |.||.:   ...||  ||:        .||            .:..:::.|..::...|..|.|:
  Fly   441 GELRFNRQLEEVNPLQNIR--------CYP------------HSFHSINITFDSRTLTSMFVVYI 485

  Fly   624 IEMFGKNETDGWVAVG-TRVQNTTFTQTGL-LPGVNYFFLI------RAENSHGLSLPSPM---- 676
            :    :|....|.:.. .::..|::..... ||....|.||      ..|...|..:|..|
  Fly   486 V----RNNPHSWTSFSPMKLNQTSYVVISTDLPVYEPFALITRVLFPTTEVRTGTLVPQQMILSS 546

  Fly   677 --SEPITVGTRYFNSGLDLSEARASLLSGDV-VELSNASVVDSTSMKLTW----------QIING 728
              |.|:|..|:    ||.|   |...:..|. |:.|...:.:.....|.:          |::||
  Fly   547 LRSTPVTCCTQ----GLML---RPIAVGNDTFVKWSQGQLENKKYFILQFSINHTHPHPAQLLNG 604

  Fly   729 KYVEGFYVYARQLPNPI---------VNNPAPVT-----SNTNPLLGST--------STSASASA 771
            . ::|......:..:.:         :|..|..|     .:.:.::..|        |....||.
  Fly   605 S-LQGTLTSVEEKADQVRHHLTEIQPLNQSAAATVLRNVEHEDNVVDDTLELEDDIFSLVVDASV 668

  Fly   772 SASAL------------ISTKPNIAAAGKR--------DGETNQSGGGAPTPLNTK------YRM 810
            :...|            |:|:........|        :|.:.||  ..|..|.|:      ::.
  Fly   669 TGILLQRFTRIKMRVLIITTENEFLGQDFRYVQWKIIENGTSEQS--NMPFRLVTRESRALAFKF 731

  Fly   811 LTILNGGGASSC---------------------------TITGLVQYTLYEFFIVP-----FYKS 843
            |..||......|                           .:|||...|.|......     ||..
  Fly   732 LDSLNETCVLECHTEIQAQMSLPSQDPKCQDRNIANSLLLVTGLKPDTRYNLHFYNCKTRIFYGD 796

  Fly   844 VEGKPSNSRIARTLEDVPSEAPYGMEALLLNSSAVFLKWKAPELKDRHGVLLNYHVIVRGIDTAH 908
            ::.|        |.:|.|...   ..:.|:..:.:.|.|:.|         :|.:..|...|...
  Fly   797 IDEK--------TEQDPPGTI---SNSKLIKQNGLKLMWEPP---------INPNGRVHHYDIRW 841

  Fly   909 NFSRILTNVTIDA-----ASPTLVLANLTEGVMYTVGVAAGNNAGVGPYCVPATLRLDPITKRLD 968
            :    |.|||.:|     ...:....|::|.....|.|....:.|.|     |.:..|
  Fly   842 S----LDNVTHEAIVKECTKCSYKFPNVSETAKIHVAVRVMGDTGAG-----APMYFD------- 890

  Fly   969 PFINQRYPINQDHVNDVLTQPWFIILLGAILAV-LMLSFGAMVFVKRKHM--------------- 1017
             .||..:...::......::.:..|.:|::|:: .:..||..:..::::.
  Fly   891 -MINNNHTWLKEESESSHSEVYSGIAIGSLLSIACVFLFGLFIIFQQRNFKARAQGPTHLTTPTD 954

  Fly  1018 -----------MMKQSALNTMRGNHTSDVLKMPSLSAR--------------------------- 1044
                       |...||.....|....|..:|.:|..|
  Fly   955 INFGNLSSASGMPGDSAGGLSGGTLQQDCHEMQTLIPRSRYHIELLPEHSLEYQTSTAAVAVVHL 1019

  Fly  1045 -NGNGYWLDSSTGGMVWRPS-----PGGDSLEMQKDHIADYAPVCGAPGS--------------- 1088
             ||||       ...|.|.|     ..|| |.:...|.....|:||..|:
  Fly  1020 ANGNG-------SVQVARRSDQDGAAQGD-LGIAGVHNLSQLPLCGVGGTSPERKTVQDAMLQEA 1076

  Fly  1089 -------------------------PAGGG-------------TSSGGSGGAGSGASGGDDIHGG 1115
                                     |:|..             |:..|.|..|.|.|||    ||
  Fly  1077 KAFYIPYRHTPPNAVALSSSKQCAVPSGSELEVIVPPNSLPLVTTMPGLGVVGGGGSGG----GG 1137

  Fly  1116 HGSERNQQRYVGEYSNIPTDYAEVSSFGKAPSEYG----RHGNASPAPY-----ATSSILSPHQQ 1171
            .|....:.      .::.:..|..||.|.:..::.    ||.|::...|     ..::.|:|..:
  Fly  1138 VGGSSRRS------GSLSSSSASSSSNGSSKKQFHTNFVRHSNSNDGIYLLAEEEAAATLTPTSE 1196

  Fly  1172 QQQQQPRYQQRPVPGY-------GLQRPMHPHYQQQQHQQQQAQQTHQQHQALQQHQQLPPSNIY 1229
            ::             |       |.:.|           ...|:.....:.....:..  .:|..
  Fly  1197 RE-------------YAAVCLTNGFKLP-----------ADVAKSGGGTYNTTNNNNS--STNTS 1235

  Fly  1230 QQMSTTSEIYPTNTGPSRSVYSEQYYYPKDKQRHIHITENKLSNCHTYEAAPGAKQSSPISSQFA 1294
            |:.:|..|..|...|...|........|.:....:::..:.           ..|||.|.    |
  Fly  1236 QKSATAKERQPRGNGNPISFSHNACKVPANGPASVNVNHSS-----------AVKQSWPA----A 1285

  Fly  1295 SV--RRQQLPPN 1304
            :|  ||.|:.||
  Fly  1286 TVVHRRAQVDPN 1297

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317
		I-set	91..184	CDD:254352
		I-set	190..279	CDD:254352	15/58 (26%)
		Ig2_Robo	193..279	CDD:143201	15/58 (26%)
		I-set	283..370	CDD:254352	25/96 (26%)
		Ig	300..370	CDD:299845	18/73 (25%)
		Ig	388..>457	CDD:299845	15/78 (19%)
		I-set	479..561	CDD:254352	25/90 (28%)
		Ig	496..561	CDD:143165	21/73 (29%)
		FN3	588..681	CDD:238020	19/106 (18%)
		FN3	<817..856	CDD:238020	9/70 (13%)
		fn3	864..952	CDD:278470	18/92 (20%)
plum	NP_001163744.1	IG_like	170..241	CDD:214653	18/74 (24%)
		IGc2	175..235	CDD:197706	15/63 (24%)
		IG_like	284..356	CDD:214653	14/74 (19%)
		I-set	360..443	CDD:254352	28/96 (29%)
		IGc2	378..435	CDD:197706	20/64 (31%)
		FN3	804..886	CDD:238020	20/102 (20%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45452283
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	2	1.840