Protein Alignment robo2 and robo1

DIOPT Version :9

• Sequence 1: NP_001259868.1
• Gene: robo2 / 44522
• FlyBaseID: FBgn0002543
• Length: 1519
• Species: Drosophila melanogaster
• Sequence 2: NP_476899.1
• Gene: robo1 / 37603
• FlyBaseID: FBgn0005631
• Length: 1395
• Species: Drosophila melanogaster
• Alignment Length: 1549
• Identity: 466/1549 - (30%)
• Similarity: 707/1549 - (45%)
• Gaps: 339/1549 - (21%)

  Fly    63 SRRAVFP--LLLLLAGLNGLTQVGALKG--ENPRIIEHPMDTTVPKNDPFTFNCQAEGNPTPTIQ 123
            ||..:.|  |||:|...|||.   |::|  ::|||||||.|..|.||:|.|.||:.||.|.|||:
  Fly    27 SRMWLLPAWLLLVLVASNGLP---AVRGQYQSPRIIEHPTDLVVKKNEPATLNCKVEGKPEPTIE 88

  Fly   124 WFKDGRELKT-DTGSHRIMLPAGGLFFLKVIHSRRESDAGTYWCEAKNEFGVARSRNATLQVAFL 187
            |||||..:.| :..|||:....|.|||.:.:..::|.|.|.|||.|||..|.|.||:|:||:|.|
  Fly    89 WFKDGEPVSTNEKKSHRVQFKDGALFFYRTMQGKKEQDGGEYWCVAKNRVGQAVSRHASLQIAVL 153

  Fly   188 RDEFRLEPANTRVAQGEVALMECGAPRGSPEPQISWRKNG------QTLNLVGNKRIRIVDGGNL 246
            ||:||:||.:||||:||.||:|||.|:|.|||.:.|.|:|      :.::...:.|:||||||||
  Fly   154 RDDFRVEPKDTRVAKGETALLECGPPKGIPEPTLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNL 218

  Fly   247 AIQEARQSDDGRYQCVVKNVVGTRESATAFLKVHVRPFLIRGPQNQTAVVGSSVVFQCRIGGDPL 311
            .|......|:|.|:|:.:|:||||||:.|.|.|.|:|:.::.|::|..:.|.:..|.|.:||||.
  Fly   219 LISNVEPIDEGNYKCIAQNLVGTRESSYAKLIVQVKPYFMKEPKDQVMLYGQTATFHCSVGGDPP 283

  Fly   312 PDVLWRRTASGGNMPLRRVHVLED-RSLKLDDVTLEDMGEYTCEADNAVGGITATGILTVHAPPK 375
            |.|||::  ..||:|:.|..:|.| :||::.::|..|.|.|.|||.|.||.|:|...|.|||||.
  Fly   284 PKVLWKK--EEGNIPVSRARILHDEKSLEISNITPTDEGTYVCEAHNNVGQISARASLIVHAPPN 346

  Fly   376 FVIRPKNQLVEIGDEVLFECQANGHPRPTLYWSVEGNSSLLLPGYRDGRMEVTLTPEGRSVLSIA 440
            |..||.|:.|.:...|...|.|:|:|.|:::|:.||.|:|:.|....||..|.    ....|.|.
  Fly   347 FTKRPSNKKVGLNGVVQLPCMASGNPPPSVFWTKEGVSTLMFPNSSHGRQHVA----ADGTLQIT 407

  Fly   441 RFAREDSGKVVTCNALNAVGSVSSRTVVSVDTQFELPPPIIEQGPVNQTLPVKSIVVLPCRTLGT 505
            ...:||.|..| |:|.:.|.|.:.|..:.|.:..|.|||||:.||.|||||..|:..||||..|.
  Fly   408 DVRQEDEGYYV-CSAFSVVDSSTVRVFLQVSSLDERPPPIIQIGPANQTLPKGSVATLPCRATGN 471

  Fly   506 PVPQVSWYLDGIPIDVQEHERRNLSDAGALTISDLQRHEDEGLYTCVASNRNGKSSWSGYLRLDT 570
            |.|::.|:.||..  ||...|.::....:|.:.|||. .|.|.|||.||...|::||:..|.::.
  Fly   472 PSPRIKWFHDGHA--VQAGNRYSIIQGSSLRVDDLQL-SDSGTYTCTASGERGETSWAATLTVEK 533

  Fly   571 PTNPNIKFFRAPELSTYPGPPGKPQMVEKGENSVTLSWTRSNKVGGS--SLVGYVIEMFGKNETD 633
            |.:.::  .||.:.||||.|||.|:::.....|::|.|.:|.:..|:  .::||.:|.|..:...
  Fly   534 PGSTSL--HRAADPSTYPAPPGTPKVLNVSRTSISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQT 596

  Fly   634 GWVAVGTRVQNTTFTQTGLLPGVNYFFLIRAENSHGLSLPSPMSEPITVGTRYFN--SGLDLSEA 696
            ||:....||.:|..|.:||.||.:|.||:||||:.|:|:||.:|..|......|:  |..|||.|
  Fly   597 GWIVAAQRVGDTQVTISGLTPGTSYVFLVRAENTQGISVPSGLSNTIKTIEADFDAASANDLSAA 661

  Fly   697 RASLLSGDVVELSNASVVDSTSMKLTWQI---INGKYVEGFYVYARQLPNPIVNNPAPVTSNTNP 758
            | :||:|..|||.:||.:::::::|.|.:   .:.|||||..::.:....|
  Fly   662 R-TLLTGKSVELIDASAINASAVRLEWMLHVSADEKYVEGLRIHYKDASVP-------------- 711

  Fly   759 LLGSTSTSASASASASALISTKPNIAAAGKRDGETNQSGGGAPTPLNTKYRMLTILNGGGASSCT 823
                                                          :.:|..:|::: ..|.|..
  Fly   712 ----------------------------------------------SAQYHSITVMD-ASAESFV 729

  Fly   824 ITGLVQYTLYEFFIVPFYKSVEGKPSNSRIARTLEDVPSEAPYGMEALLLNSSAVFLKWKAPELK 888
            :..|.:||.||||:.||::::||:||||:.|.|.|||||..|..::..:.|.:|.:::|..|..:
  Fly   730 VGNLKKYTKYEFFLTPFFETIEGQPSNSKTALTYEDVPSAPPDNIQIGMYNQTAGWVRWTPPPSQ 794

  Fly   889 DRHGVLLNYHVIVRGIDTAHNFSRILTNVTIDAASPTLVLANLTEGVMYTVGVAAGNNAGVGPYC 953
            ..:|.|..|.:.|    :|.|..::|.|:|::|.:.:::|.|||.|.:|:|.:.:...||.|||.
  Fly   795 HHNGNLYGYKIEV----SAGNTMKVLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYS 855

  Fly   954 VPATLRLDPITKRLDP-----------------------------FINQRYPINQDHVNDVLTQP 989
            .|.:|.:|| |..:.|                             .||   |.......|.|:.|
  Fly   856 KPISLFMDP-THHVHPPRAHPSGTHDGRHEGQDLTYHNNGNIPPGDIN---PTTHKKTTDYLSGP 916

  Fly   990 WFIILLGAILAVLMLSFG-AMVFVKRKHMMMKQ-SALNTMRGNHTSDVLKMPSLSARNGNGYWLD 1052
            |.::|:..:|.||::|.. :||:.||||.|.|: ..|:.:..|      ::.:|:..:....|:|
  Fly   917 WLMVLVCIVLLVLVISAAISMVYFKRKHQMTKELGHLSVVSDN------EITALNINSKESLWID 975

  Fly  1053 SSTGGMVWRPSPGGDSLEMQKDHIADYAPVCGAPGSPAGGGTSSGGSGGAGSGASGGDDIHGGHG 1117
            ...|   ||      :.:..||                             ||.|....:...:.
  Fly   976 HHRG---WR------TADTDKD-----------------------------SGLSESKLLSHVNS 1002

  Fly  1118 SERNQQRYVGEYSNIP--TDYAEVSSFGKAPSEYGRHGNASPAPYATSSIL-------------- 1166
            |:.|       |:|..  ||||||.:.........|....:|.||||:.|:
  Fly  1003 SQSN-------YNNSDGGTDYAEVDTRNLTTFYNCRKSPDNPTPYATTMIIGTSSSETCTKTTSI 1060

  Fly  1167 ---------SPHQQQQQQQPRYQQRPVPGYG------LQRPMHPHYQQQQHQQQQAQQTHQQHQA 1216
                     ||:......|       ||...      ||.|:.|
  Fly  1061 SADKDSGTHSPYSDAFAGQ-------VPAVPVVKSNYLQYPVEP--------------------- 1097

  Fly  1217 LQQHQQLPPSNIYQQMSTTSEIYPTNTGPSRSVYSEQYYYPKDKQRHIH------ITENKLSNCH 1275
            :...:.|||              |....|..|.|......|:..::...      .|...:.|..
  Fly  1098 INWSEFLPP--------------PPEHPPPSSTYGYAQGSPESSRKSSKSAGSGISTNQSILNAS 1148

  Fly  1276 TYEAAPGAKQSSPISSQFASVRRQQLPP--------NCSIGRESARFKVLNTDQGKNQQNLLDLD 1332
            .:.::.|...:..:|.|:|..    .||        :...|....|:::..|:|...|....
  Fly  1149 IHSSSSGGFSAWGVSPQYAVA----CPPENVYSNPLSAVAGGTQNRYQITPTNQHPPQLPAY--- 1206

  Fly  1333 GSSMCYNGLADSGCGGSPSPMAMLMSHEDEHALYHTADG----------DLDDMERLYVKVDEQQ 1387
                    .|.:|.||:..|..:..:.: .||......|          ..:..:.|......|.
  Fly  1207 --------FATTGPGGAVPPNHLPFATQ-RHAASEYQAGLNAARCAQSRACNSCDALATPSPMQP 1262

  Fly  1388 PPQQQQQLIPLV------------PQHPAEGHLQSWRNQSTRSSRKNGQECIKEPSELIYAPGSV 1440
            ||.     :|:.            |.||...:.|.:             :|..|.|:  ::..|.
  Fly  1263 PPP-----VPVPEGWYQPVHPNSHPMHPTSSNHQIY-------------QCSSECSD--HSRSSQ 1307

  Fly  1441 ASERSLLSNSGSGTSSQPAGHN------VXPLLPGSSLGQSQSKSQSQSQSQSD 1488
            :.:|.|.......::.|..||:      | |.:  .|..::......|.|..||
  Fly  1308 SHKRQLQLEEHGSSAKQRGGHHRRRAPVVQPCM--ESENENMLAEYEQRQYTSD 1359

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	50/95 (53%)
		I-set	91..184	CDD:254352	49/93 (53%)
		I-set	190..279	CDD:254352	46/94 (49%)
		Ig2_Robo	193..279	CDD:143201	44/91 (48%)
		I-set	283..370	CDD:254352	35/87 (40%)
		Ig	300..370	CDD:299845	31/70 (44%)
		Ig	388..>457	CDD:299845	23/68 (34%)
		I-set	479..561	CDD:254352	36/81 (44%)
		Ig	496..561	CDD:143165	25/64 (39%)
		FN3	588..681	CDD:238020	36/94 (38%)
		FN3	<817..856	CDD:238020	18/38 (47%)
		fn3	864..952	CDD:278470	25/87 (29%)
robo1	NP_476899.1	Ig	56..151	CDD:299845	50/94 (53%)
		I-set	56..150	CDD:254352	49/93 (53%)
		I-set	157..251	CDD:254352	46/93 (49%)
		Ig2_Robo	159..251	CDD:143201	44/91 (48%)
		I-set	255..341	CDD:254352	35/87 (40%)
		Ig3_Robo	272..341	CDD:143202	31/70 (44%)
		IG_like	351..436	CDD:214653	29/89 (33%)
		Ig	362..444	CDD:299845	28/86 (33%)
		I-set	445..531	CDD:254352	39/88 (44%)
		IGc2	459..521	CDD:197706	25/64 (39%)
		FN3	549..637	CDD:238020	33/87 (38%)
		FN3	673..762	CDD:238020	30/149 (20%)
		fn3	770..855	CDD:278470	26/88 (30%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6635
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D173520at2759
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	P	PTHR12231
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
	7	6.920