DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo2 and robo3

DIOPT Version :9

Sequence 1:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster
Sequence 2:	NP_608592.2	Gene:	robo3 / 33314	FlyBaseID:	FBgn0041097	Length:	1342	Species:	Drosophila melanogaster

Alignment Length:	1493	Identity:	615/1493 - (41%)
Similarity:	829/1493 - (55%)	Gaps:	302/1493 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    91 PRIIEHPMDTTVPKNDPFTFNCQAEGNPTPTIQWFKDGRELKTDTGSHRIMLPAGGLFFLKVIHS 155
            |||:|||:|||||:::|.|.||:|||:|||||||:|||..||...|||||.|||||||||||::|
  Fly    23 PRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPLKILPGSHRITLPAGGLFFLKVVNS 87

  Fly   156 RRESDAGTYWCEAKNEFGVARSRNATLQVAFLRDEFRLEPANTRVAQGEVALMECGAPRGSPEPQ 220
            |||:|||.||||||||.|||||||||||||.|||||||||.|||:|||:.||:||.||||.|||.
  Fly    88 RRETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECAAPRGIPEPT 152

  Fly   221 ISWRKNGQTLNLVGNKRIRIVDGGNLAIQEARQSDDGRYQCVVKNVVGTRESATAFLKVHVRPFL 285
            ::|:|.||.|:|.|:||:|||||||||||:|||:|:|:|||:.||.||.|||:.|.|||||:|::
  Fly   153 VTWKKGGQKLDLEGSKRVRIVDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLATLKVHVKPYI 217

  Fly   286 IRGPQNQTAVVGSSVVFQCRIGGDPLPDVLWRRTASGGNMPLRRVHVLEDRSLKLDDVTLEDMGE 350
            ||||.:||.:.|:||.|.||:||||:|||||.||||||||||.||.|||||||:|:.||:.|.||
  Fly   218 IRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLEDRSLRLERVTIADEGE 282

  Fly   351 YTCEADNAVGGITATGILTVHAPPKFVIRPKNQLVEIGDEVLFECQANGHPRPTLYWSVEGNSSL 415
            |:|||||.||.|||.|.|||:|||||:.||.::.||:|.:..|||:|.|:|:||::|:::.||:|
  Fly   283 YSCEADNVVGAITAMGTLTVYAPPKFIQRPASKSVELGADTSFECRAIGNPKPTIFWTIKNNSTL 347

  Fly   416 LLPGYRD-GRMEVTLTPEGRSVLSIARFAREDSGKVVTCNALNAVGSVSSRTVVSVDTQFELPPP 479
            :.||... .|.....|.||.|:|::.||.|.|...|:.|||:|.|.|::||..:|:|:|.:.|||
  Fly   348 IFPGAPPLDRFHSLNTEEGHSILTLTRFQRTDKDLVILCNAMNEVASITSRVQLSLDSQEDRPPP 412

  Fly   480 IIEQGPVNQTLPVKSIVVLPCRTLGTPVPQVSWYLDGIPIDVQEHERRNLSDAGALTISDLQRHE 544
            ||..||||||||:||:..|.|:.:|.|.|.:|||.||||  ||...:.|::.:|.|.||||.|.:
  Fly   413 IIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDGIP--VQPSSKLNITTSGDLIISDLDRQQ 475

  Fly   545 DEGLYTCVASNRNGKSSWSGYLRLDTPTNPNIKFFRAPELSTYPGPPGKPQMVEKGENSVTLSWT 609
            |:||||||||:|.|||:|||:||::.||||||||:||||.:..|..||:|:::....:::|:.|.
  Fly   476 DQGLYTCVASSRAGKSTWSGFLRIELPTNPNIKFYRAPEQTKCPSAPGQPKILNATASALTIVWP 540

  Fly   610 RSNKVGGSSLVGYVIEMFGKNETDGWVAVGTRVQNTTFTQTGLLPGVNYFFLIRAENSHGLSLPS 674
            .|:|.|.||.:||.:||:..|::..|:.:.:|:....||...|..|..|.|::|||||.|.|.||
  Fly   541 TSDKAGASSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVESLTQGAAYMFIVRAENSLGFSPPS 605

  Fly   675 PMSEPIT----VGTR-----YFNSGLDLSEARASLLSGDVVELSNASVVDSTSMKLTWQIINGKY 730
            |:|||||    ||.|     ...|.|.||:....|.:.|||||..|:..|||:.:|:|.|.:|:|
  Fly   606 PISEPITAGKLVGVRDGSESTGTSQLLLSDVETLLQANDVVELLEANASDSTTARLSWDIDSGQY 670

  Fly   731 VEGFYVYARQLPNPIVNNPAPVTSNTNPLLGSTSTSASASASASALISTKPNIAAAGKRDGETNQ 795
            :||||:|||:|.
  Fly   671 IEGFYLYARELH----------------------------------------------------- 682

  Fly   796 SGGGAPTPLNTKYRMLTILN-GGGASSCTITGLVQYTLYEFFIVPFYKSVEGKPSNSRIARTLED 859
                     :::|:|:|:|| |.|.||||:.||.:.:.||||:||||||:.|||||||..|||||
  Fly   683 ---------SSEYKMVTLLNKGQGLSSCTVPGLAKASTYEFFLVPFYKSIVGKPSNSRRMRTLED 738

  Fly   860 VPSEAPYGMEALLLNSSAVFLKWKAPE-LKDRHGVLLNYHVIVRGIDTAHNFSRILTNVTIDAAS 923
            ||...||||||:..|.::|||||..|: .:.|:|:|.:|:|:|:|:| .||.:||..|:|||||:
  Fly   739 VPEAPPYGMEAIQFNRTSVFLKWLPPQPNRTRNGILTSYNVVVKGLD-VHNTTRIFKNMTIDAAA 802

  Fly   924 PTLVLANLTEGVMYTVGVAAGNNAGVGPYCVPATLRLDPITKRLDPFINQRYPINQDHVNDVLTQ 988
            |||:|||||.||.|.:.|||....||||:..||.||:|..|:.||.... ||||::|..:|.|||
  Fly   803 PTLLLANLTTGVTYYIAVAAATRVGVGPFSKPAVLRIDARTQSLDTGYT-RYPISRDIADDFLTQ 866

  Fly   989 PWFIILLGAILAVLMLSFGAMVFVKRKHMMMKQSALNTMRGNHT-SDVLKMPSL----------- 1041
            .|||:|||:|:|:::...||:|..|| :..:||::|.::.|||. ..|.|.|:|
  Fly   867 TWFIVLLGSIIAIIVFLLGALVLFKR-YQFIKQTSLGSLHGNHAIGTVRKFPTLPLNGGGAVGAG 930

  Fly  1042 -------SARNGNGYWLDSSTGGMVWRPSPGG--------------------------------- 1066
                   :.::|:|.|:|.::|  |||.:.||
  Fly   931 GSNSTAAAGQSGHGLWIDPTSG--VWRQAAGGGNPGGVGSCTTKEQLPGYAQATAQQGQQPTLLP 993

  Fly  1067 DSLEMQKDHIADYAPV-CGAPGSPAGGGTSSGGSGGAGSGASGGDDIHGGHGSER---NQQRYVG 1127
            |...:...::.|||.| |....||..|....|| ||.|..||..|.. |.:.:..   |.:.|..
  Fly   994 DYERLSPLNMPDYAEVACSTFKSPTHGAPPMGG-GGGGQSASLYDSC-GAYATTNVVANVKLYQN 1056

  Fly  1128 EYSNIPTDYAEVSSFGKAPSEYGRHGNASPAPYATSSILSPHQQQQQQQPRYQQRPVPGYGLQRP 1192
            .|:..|...:..::.....::|...|..|..|.|....|.|.||||||||.
  Fly  1057 RYATKPNQSSGSNNNNHQGNDYQSTGMYSAPPSAHYGCLEPKQQQQQQQPN-------------- 1107

  Fly  1193 MHPHYQQQQHQQQQAQQTHQQHQALQQHQQLPPSNIYQQMSTTSEIYPTNTGPSRSVYSEQYYYP 1257
                                                ....||.|....||: |:
  Fly  1108 ------------------------------------LMTTSTASTAILTNS-PA----------- 1124

  Fly  1258 KDKQRHIHITENKLSN--------------------CHTYEAAPGAKQSSPISSQFAS------V 1296
              |.:.|:|||||:..                    .:..:...||..::.:::|.||      :
  Fly  1125 --KVKKINITENKMDQLEGKSERTNPFNQQQQLLLASNALKQGLGAYANTTLAAQMASGGGAGTL 1187

  Fly  1297 RRQQLP------PNCSIGRESARFKVLNTDQG---KNQQNLL---------DLDGSSMC------ 1337
            |||:.|      .|..:|:...|...:|...|   ..|.:.|         |..|..:|
  Fly  1188 RRQRQPKTLYKSENNILGKSGLRQNTMNASVGAPSNGQMDFLTGGPPSEGGDFSGLGLCNSTNQL 1252

  Fly  1338 YNGLADSGCGGSPSPMAMLMSHEDEHALYHTADGDLDDMERLYVKV----------DEQQPPQQQ 1392
            .|..|.|....:|..             ||..... ...:.||||.          |..|..:.|
  Fly  1253 LNDWASSASIAAPGD-------------YHFGSKQ-PSKQHLYVKAKDGTWSAVSSDAYQSFKHQ 1303

  Fly  1393 QQLIPLVPQHPAEGHLQSWRNQSTRSSRKNGQECIKEPSELIYAPGSVASERSLLSNSGSGTS 1455
            ||      .||    ..:....:|:|               :.:..|:|.:...||:.||..:
  Fly  1304 QQ------HHP----FLAGSGDNTKS---------------LASVNSLAGDSKFLSSFGSSAN 1341

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	72/93 (77%)
		I-set	91..184	CDD:254352	71/92 (77%)
		I-set	190..279	CDD:254352	60/88 (68%)
		Ig2_Robo	193..279	CDD:143201	57/85 (67%)
		I-set	283..370	CDD:254352	60/86 (70%)
		Ig	300..370	CDD:299845	51/69 (74%)
		Ig	388..>457	CDD:299845	29/69 (42%)
		I-set	479..561	CDD:254352	47/81 (58%)
		Ig	496..561	CDD:143165	34/64 (53%)
		FN3	588..681	CDD:238020	37/92 (40%)
		FN3	<817..856	CDD:238020	24/38 (63%)
		fn3	864..952	CDD:278470	48/88 (55%)
robo3	NP_608592.2	Ig	22..117	CDD:299845	72/93 (77%)
		I-set	23..116	CDD:254352	71/92 (77%)
		I-set	122..211	CDD:254352	60/88 (68%)
		Ig	125..211	CDD:299845	57/85 (67%)
		I-set	215..302	CDD:254352	60/86 (70%)
		Ig	232..302	CDD:299845	51/69 (74%)
		I-set	306..401	CDD:254352	41/94 (44%)
		Ig	320..411	CDD:299845	37/90 (41%)
		I-set	412..492	CDD:254352	47/81 (58%)
		IGc2	429..489	CDD:197706	32/61 (52%)
		FN3	519..612	CDD:238020	37/92 (40%)
		FN3	644..735	CDD:238020	52/152 (34%)
		fn3	743..832	CDD:278470	49/89 (55%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45471435
Domainoid	1	1.000	104	1.000	Domainoid score	I6635
eggNOG	1	0.900	-	-		E1_KOG4222
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D14518at33392
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
	8	7.650