DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo2 and robo1

DIOPT Version :9

Sequence 1:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster
Sequence 2:	XP_017214701.2	Gene:	robo1 / 30769	ZFINID:	ZDB-GENE-000209-3	Length:	1705	Species:	Danio rerio

Alignment Length:	1646	Identity:	454/1646 - (27%)
Similarity:	701/1646 - (42%)	Gaps:	374/1646 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    41 KRRHRNNR------DFHGERLKIMPVDRSRRAVFPLLLLLAGLNGLTQVGALKGENPRIIEHPMD 99
            ::||.|::      ||...||:                             .:...|||:|||.|
Zfish    58 QQRHHNSKKHKCFQDFQRSRLR-----------------------------QEDYAPRIVEHPSD 93

  Fly   100 TTVPKNDPFTFNCQAEGNPTPTIQWFKDGRELKTDTG---SHRIMLPAGGLFFLKVIHSRR-ESD 160
            ..|.|.:|.|.||:|||.||||::|:|||..::||..   |||::||.|.||||:::|.|| :.|
Zfish    94 LIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDRDNPRSHRMLLPTGSLFFLRIVHGRRSKPD 158

  Fly   161 AGTYWCEAKNEFGVARSRNATLQVAFLRDEFRLEPANTRVAQGEVALMECGAPRGSPEPQISWRK 225
            .|:|.|.|:|..|.|.|.||:|:||.|||:||..|.:..||.||.|:|||..|||.|||.|||:|
Zfish   159 EGSYVCVARNYLGEAVSHNASLEVAILRDDFRQNPVDVMVAAGEPAVMECQPPRGHPEPTISWKK 223

  Fly   226 NGQTLNLVGNKRIRI-VDGGNLAIQEARQSDDGRYQCVVKNVVGTRESATAFLKVHVRPFLIRGP 289
            :|..::   ::..|| :.||.|.|...|::|.|:|.||..|::|.|||..|.|.|..||..:|.|
Zfish   224 DGVNID---DRDERITIRGGKLMITNTRKTDAGKYVCVGTNMMGERESEIAELTVLERPSFLRKP 285

  Fly   290 QNQTAVVGSSVVFQCRIGGDPLPDVLWRRTASGGNMPLRRVHVLEDRSLKLDDVTLEDMGEYTCE 354
            .:|:.:|..||.|:|...|||:|.:.||:  ..|.:|..|..:.||.:|||..:|..|.|.|||:
Zfish   286 SSQSVLVDQSVEFRCEAQGDPVPTIRWRK--EDGELPKGRYEIWEDHTLKLKRLTSGDAGTYTCQ 348

  Fly   355 ADNAVGGITATGILTVH---APPKFVIRPKNQLVEIGDEVLFECQANGHPRPTLYWSVEGNSSLL 416
            |:|.:|...|:..||||   .||.||:||:||:..:|..|.|:|:|.|.|:|.::|..||:.:||
Zfish   349 AENMMGKTEASATLTVHVVSVPPVFVVRPRNQVAGMGRTVTFQCEATGSPQPAIFWQKEGSQNLL 413

  Fly   417 L---PGYRDGRMEVTLTPEGRSVLSIARFAREDSGKVVTCNALNAVGSVSSRTVVSV-DTQFELP 477
            .   |.....|..|:.|.:    |:|....|.|.| ..:|.|||..|||.::.::.| |...:.|
Zfish   414 FSSQPPPPSSRFSVSQTGD----LTITAVERSDGG-YYSCQALNIAGSVITKALLEVTDVISDRP 473

  Fly   478 PPIIEQGPVNQTLPVKSIVVLPCRTLGTPVPQVSWYLDGIPIDVQEHERRNLSDAGALTISDLQR 542
            |||:.|||.|||:.|...|||.|:..|||:|.:.|..||:.:...:...:.| |.|||.|. ..:
Zfish   474 PPIVRQGPTNQTVAVDGTVVLSCQATGTPIPTILWRKDGVLVSTHDSRLKQL-DTGALQIR-YAK 536

  Fly   543 HEDEGLYTCVASNRNGKSSWSGYLRLD---------TPTNPNIKFFRAPELSTYPGPPGKPQMVE 598
            ..|.|:|||.||..:|::||..||.:.         .||:||:          .|..|.||.:.:
Zfish   537 LGDTGMYTCTASTPSGEASWKAYLEVQEFGAPVQPGRPTDPNL----------IPSAPSKPDVTD 591

  Fly   599 KGENSVTLSWTRSNKVGGSSLVGYVIEMFGKNETDGWVAVGTRVQNTTFTQTGLLPGVNYFFLIR 663
            ....||:||| :.|...|::...||||.|.......|..:...|:..:|...||.|...|.||:|
Zfish   592 VSRTSVSLSW-KPNLNAGATPTSYVIEAFSHAAGSSWQTLADHVKTESFVLKGLKPSAVYLFLVR 655

  Fly   664 AENSHGLSLPSPMSEPI-TVGTRYFNSGLDLSEARASLLSGDVV-ELSNASVVDSTSMKLTWQI- 725
            |.|::|||.|||:|:.: |......:.|::..:.:..|  |||| .|.|.:::.|:|:::.|.:
Zfish   656 AANAYGLSDPSPISDAVKTQDVPPTSQGVEHGQIQREL--GDVVIHLHNPTILSSSSVRVQWTVE 718

  Fly   726 INGKYVEGFYVYARQLPNPIVNNPAPVTSNTNPLLGSTSTSASASASASALISTKPNIAAAGKRD 790
            ...:|::|:.|..|..|.
Zfish   719 QQSQYIQGYKVMYRPSPE----------------------------------------------- 736

  Fly   791 GETNQSGGGAPTPLNTKYRMLTILNGGGASSCTITGLVQYTLYEFFIVPFYKSVEGKPSNSRIAR 855
                    |||..::  :.|..: ...|..|..:|.|.:...|||.:.||:...:|..|:.::.:
Zfish   737 --------GAPQRVD--WAMFEV-GAPGEHSAVVTQLKKGITYEFKVRPFFNEFQGTDSDIKVGK 790

  Fly   856 TLEDVPSEAPYGMEALLL--NSSAVFLKWKAPELKDRHGVLLNYHVIVRGIDTAHNFSRILTNVT 918
            |||:.||.||.|:.....  |.:||.:.|:.|..::::||:..|.:...|     |.||...|.|
Zfish   791 TLEEAPSAAPRGVTVTGSGDNGTAVLVAWQPPPEEEQNGVVQEYKIWCLG-----NESRYHINRT 850

  Fly   919 IDAASPTLVLANLTEGVMYTVGVAAGNNAGVGPYCVPATLRLDPITKRLDPFINQRYPINQDHVN 983
            :|.::.::::..|..||.|.:.||||..||.|....|.:.:||...:.|.|.::....|:| .::
Zfish   851 VDGSTHSVLIPGLVAGVTYRLEVAAGTGAGPGVKSDPTSFQLDSSGRVLPPPLDDDNTISQ-QIS 914

  Fly   984 DVLTQPWFIILLGAILAVLMLSFGAMVFVKRKHMMMKQSALNTMRGNHTSDVLKMPSLS------ 1042
            ||:.||.||..:||...::::.|...::..||    |::.|:    :..:.:.|:||.:
Zfish   915 DVVRQPAFIAGIGAACWIILMVFSIWLYRHRK----KRNGLS----SSYAGIRKVPSFTFTPTVA 971

  Fly  1043 -ARNGNGYWLDSSTGGMVWRPSPGGDSLEMQKDHIADYAPVCGAPGSPAGGGTSSGGSGGAGSGA 1106
             .|.|...   ||.|    ||..........:..:||..|...:..:.......:|.:|.:.|..
Zfish   972 YQRGGEAV---SSAG----RPGLLNIGEPATQPWLADTWPNSCSNHNDCTINCCTGSNGNSDSNL 1029

  Fly  1107 ---SGGDDIHGGHGSERNQQRY------VGEYSNI--PTDYAEVSSFGKAPSEYGRH--GNASPA 1158
               |...|....:.::.:.:..      .|.||::  .....|:.:|.....:.||.  ....|.
Zfish  1030 ATYSRPADCIANYSNQMDNKATNLLAPDTGLYSDVDLSNKINEMKTFNSPNLKDGRFMGPGGQPT 1094

  Fly  1159 PYATSSIL-------------------SPHQQQQQQQPRYQQRPVPGYGLQRPMHPHYQQQQHQQ 1204
            ||||:.::                   |....::|.....|..|.....:..|:    |....:|
Zfish  1095 PYATTQLIQSSLANNNMNSSSGGSGGGSADFNEKQSWKSSQGTPQKQADINTPL----QYNVMEQ 1155

  Fly  1205 QQAQQTHQQHQALQQHQQLP-----PSNIYQQMSTTSEIYPTNTGPSRSVYSEQYYYPKDKQRHI 1264
            .:..:.|.:.:.       |     |.|..|.:.|......::.|.|.|....|      |:...
Zfish  1156 NKLNKDHYRGEG-------PISVTIPYNQAQDVLTGETYSSSDRGSSTSGSQGQ------KKGGR 1207

  Fly  1265 HITENKLSNCHTYEAAPGAKQSSPISSQFASVRRQQ--------LPPNCSIGRESARFKVLNTDQ 1321
            ...:.|.:..:..|..|....:.|...::..|....        |||:         ...|..|:
Zfish  1208 TTKQAKQNPMNWTELLPPPPVNPPPCQEYTLVMDDSYDTDLQCPLPPS---------RMYLQPDE 1263

  Fly  1322 GKNQQNLLDLDGSSMCYNGLADSGCGGSPSPMAMLMSHEDEHALYHTADGDLDDM--------ER 1378
            .:.:::.::|:      .|......|.:.||.|:..||:....|..:...::..|        ||
Zfish  1264 LEEEEDEVELE------RGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEEMHPMLQDNPDLHER 1322

  Fly  1379 LYVKVDEQQPPQQQQQLIPLVP------------------------------------------- 1400
            ..:.|....||:      ||.|
Zfish  1323 RRLLVSPPPPPR------PLSPPHTYGYISSPLGLDTDGLEEEDDDEEEGDETDAEVARVHQHHM 1381

  Fly  1401 QHPAEGHLQSWR------NQSTRSSRKNGQECIKEPS---ELIYAPGSVASERSLLSNSGSGTSS 1456
            .||.:.||...|      .|:..||     .|..|.|   .:|...||.:.|.:..|...|..||
Zfish  1382 MHPHQQHLPQQRALLRGLEQTPASS-----TCDLESSVTGSMINGWGSASEEDNASSGRSSAVSS 1441

  Fly  1457 Q------------------------------------------------PAGHNVXPLLPGSSLG 1473
            .                                                ||||.     |||.:.
Zfish  1442 SDGSFFTDADFAQAVAAAAEYAGLRVAKHPGKGHHQLPVSGAARKYQNLPAGHR-----PGSPVS 1501

  Fly  1474 QSQSKSQSQSQSQSDLQSQEQ 1494
            ...:.|.:..| :..|:.|:|
Zfish  1502 TDSNVSAAPGQ-RRPLKRQKQ 1521

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	51/98 (52%)
		I-set	91..184	CDD:254352	51/96 (53%)
		I-set	190..279	CDD:254352	41/89 (46%)
		Ig2_Robo	193..279	CDD:143201	39/86 (45%)
		I-set	283..370	CDD:254352	34/86 (40%)
		Ig	300..370	CDD:299845	28/69 (41%)
		Ig	388..>457	CDD:299845	24/71 (34%)
		I-set	479..561	CDD:254352	34/81 (42%)
		Ig	496..561	CDD:143165	25/64 (39%)
		FN3	588..681	CDD:238020	35/93 (38%)
		FN3	<817..856	CDD:238020	11/38 (29%)
		fn3	864..952	CDD:278470	29/89 (33%)
robo1	XP_017214701.2	Ig1_Robo	84..183	CDD:143317	51/98 (52%)
		Ig	191..275	CDD:325142	39/86 (45%)
		Ig3_Robo	296..363	CDD:143202	27/68 (40%)
		Ig	385..474	CDD:325142	31/93 (33%)
		Ig_3	474..548	CDD:316449	32/75 (43%)
		FN3	581..672	CDD:238020	35/91 (38%)
		FN3	704..791	CDD:238020	23/144 (16%)
		fn3	799..885	CDD:306538	29/90 (32%)
		Herpes_ICP4_C	1491..>1653	CDD:332854	12/37 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6635
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	O	PTHR12231
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	7	6.910