DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment robo2 and Ncam2

DIOPT Version :9

Sequence 1:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster
Sequence 2:	NP_981954.2	Gene:	Ncam2 / 288280	RGDID:	1303131	Length:	837	Species:	Rattus norvegicus

Alignment Length:	604	Identity:	147/604 - (24%)
Similarity:	239/604 - (39%)	Gaps:	83/604 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly   110 FNCQAEGNPTPTIQWFKDGRELKTDTGSHRIMLPAGGLFFLKVIHSRRESDAGTYWCEAKNEFGV 174
            |.|.|.|.| .:|.|:....|....|  .|:||...|:.....|::....|||.|.|:|.:..|.
  Rat    40 FTCTAIGEP-ESIDWYNPQGEKIIST--QRVMLQKEGVRSRLTIYNANIEDAGIYRCQATDAKGQ 101

  Fly   175 ARSRNATLQVAFLRDEFR--LEPANTRVAQGEVALMECGAPRGSPEPQISWRKNGQTLNLVGNKR 237
            .:.....|:: :.:..||  |.|...:  |||.|.:.|.. ..||.|.:||..:.:.:..:.:.|
  Rat   102 TQEATVVLEI-YQKLTFREVLSPQEFK--QGEDAEVVCRV-SSSPAPAVSWLYHNEEVTTIPDNR 162

  Fly   238 IRIVDGGNLAIQEARQSDDGRYQCVVK-NVVGTRESATAFLKVHVRPFLIRGPQ---NQTAVVGS 298
            ..::...||.|....:||:|.|:|..: ...|..:.....:.|:|.|.::. ||   |.||..|.
  Rat   163 FAVLANNNLQILNINKSDEGIYRCEGRVEARGEIDFRDIIVIVNVPPAIVM-PQKSFNATAERGE 226

  Fly   299 SVVFQCRIGGDPLPDVLWRRTASGGNMPLRRVHVLE--DRSLKLDDVTLEDMGEYTCEADNAVGG 361
            .:...|:..|.|.|.:.|.|  :|..:.....::|:  :..|.:.::..:|.|.|.|:|.|..|.
  Rat   227 EMTLTCKASGSPDPAISWFR--NGKLIEENEKYILKGSNTELTVRNIINKDGGSYVCKATNKAGE 289

  Fly   362 ITATGILTVHAPPKFVIRPKNQLVEIGDEVLFECQANGHPRPTLYWS--------VEGNSSLLLP 418
            ......|.|...| .:::.||:.......|...|:|.|.|.|.:.|.        .||:.|
  Rat   290 DQKQAFLQVFVQP-HILQLKNETTSENGHVTLICEAEGEPVPEITWKRAIDGVTFSEGDKS---- 349

  Fly   419 GYRDGRMEVTLTPEGRSVLSIARFAREDSGKVVTCNALNAVGSVSSRTVVSVDTQFELPPPIIEQ 483
              .|||:||. ...|||.|.|......|||: ..|.|.:.:|....    |:....|..|..:.
  Rat   350 --PDGRIEVK-GQHGRSSLHIRDVKLSDSGR-YDCEAASRIGGHQR----SMHLDIEYAPKFVS- 405

  Fly   484 GPVNQTL-------PVKSIVVLPCRTLGTPVPQVSWYLDGIPIDVQEHERRNLSDAGALTISDLQ 541
               |||:       |:.    :.|.....|...:.|..:.:.:..:..........|...|.::.
  Rat   406 ---NQTMYYSWEGNPIN----ISCDVKANPPASIHWRREKLVLPAKNTTHLKTHSVGRKMILEIA 463

  Fly   542 RHEDE--GLYTCVASNRNGKSSWSGYLRLDTPTNPNIKFFRAPELSTYPGPPGKPQMVEKGENSV 604
            ...|.  |.|.|.|:||.| :.:..|:.               ||:..|..|...:::|..:.:.
  Rat   464 PTSDNDFGRYNCTATNRIG-TRFQEYIL---------------ELADVPSSPRGVKIIELSQTTA 512

  Fly   605 TLSWTRSNKVGGSSLVGYVIEMFGKNET-DGWVAVGTRVQNTTFTQTGLLPGVNYFFLIRAENSH 668
            .:|:.:....||..:..|.:::  |..| :.|..|.:....||...:.|.|...|...:.|.|..
  Rat   513 KISFNKPESHGGVPIHHYQVDV--KEVTSETWKIVRSHGVQTTVVLSSLEPNTTYEVRVAAVNGK 575

  Fly   669 G--------LSLPSPMSEP 679
            |        :....|:.||
  Rat   576 GQGDYSKIEIFQTLPVREP 594

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	22/74 (30%)
		I-set	91..184	CDD:254352	22/73 (30%)
		I-set	190..279	CDD:254352	24/91 (26%)
		Ig2_Robo	193..279	CDD:143201	22/86 (26%)
		I-set	283..370	CDD:254352	24/91 (26%)
		Ig	300..370	CDD:299845	17/71 (24%)
		Ig	388..>457	CDD:299845	25/76 (33%)
		I-set	479..561	CDD:254352	17/90 (19%)
		Ig	496..561	CDD:143165	13/66 (20%)
		FN3	588..681	CDD:238020	22/101 (22%)
		FN3	<817..856	CDD:238020
		fn3	864..952	CDD:278470
Ncam2	NP_981954.2	IgI_1_NCAM-2	21..113	CDD:409452	22/76 (29%)
		Ig strand B	38..42	CDD:409452	1/1 (100%)
		Ig strand C	50..54	CDD:409452	1/3 (33%)
		Ig strand E	76..80	CDD:409452	0/3 (0%)
		Ig strand F	90..95	CDD:409452	2/4 (50%)
		Ig strand G	104..107	CDD:409452	0/2 (0%)
		IGc2	128..189	CDD:197706	19/61 (31%)
		Ig strand B	130..139	CDD:409353	3/9 (33%)
		Ig strand C	145..153	CDD:409353	2/7 (29%)
		Ig strand C'	156..159	CDD:409353	0/2 (0%)
		Ig strand D	162..167	CDD:409353	1/4 (25%)
		Ig strand E	168..175	CDD:409353	3/6 (50%)
		IgI_1_MuSK	209..298	CDD:409562	24/91 (26%)
		Ig strand B	228..232	CDD:409562	0/3 (0%)
		Ig strand C	241..245	CDD:409562	0/3 (0%)
		Ig strand E	264..268	CDD:409562	1/3 (33%)
		Ig strand F	278..283	CDD:409562	2/4 (50%)
		Ig strand G	291..294	CDD:409562	0/2 (0%)
		Ig	300..397	CDD:416386	30/109 (28%)
		Ig strand A	300..305	CDD:409353	1/5 (20%)
		Ig strand A'	309..313	CDD:409353	1/3 (33%)
		Ig strand B	317..325	CDD:409353	2/7 (29%)
		Ig strand C	331..337	CDD:409353	1/5 (20%)
		Ig strand C'	340..343	CDD:409353	0/2 (0%)
		Ig strand D	353..359	CDD:409353	3/6 (50%)
		Ig strand E	362..368	CDD:409353	3/5 (60%)
		Ig strand F	376..384	CDD:409353	3/8 (38%)
		Ig strand G	387..397	CDD:409353	2/13 (15%)
		Ig_3	401..479	CDD:404760	15/85 (18%)
		Ig strand B	418..422	CDD:409353	0/7 (0%)
		Ig strand C	431..435	CDD:409353	0/3 (0%)
		Ig strand E	458..462	CDD:409353	1/3 (33%)
		Ig strand F	472..477	CDD:409353	2/4 (50%)
		Ig strand G	486..489	CDD:409353	0/2 (0%)
		FN3	496..588	CDD:238020	19/93 (20%)
		fn3	594..678	CDD:394996	1/1 (100%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910