DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo2 and Robo2

DIOPT Version :9

Sequence 1:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster
Sequence 2:	XP_006523098.1	Gene:	Robo2 / 268902	MGIID:	1890110	Length:	1598	Species:	Mus musculus

Alignment Length:	1600	Identity:	461/1600 - (28%)
Similarity:	688/1600 - (43%)	Gaps:	359/1600 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    60 VDRSRR--AVFPLLLLLAG--LNGLTQV----------GALKGEN--PRIIEHPMDTTVPKNDPF 108
            ||:..|  .:|.|:|..:|  |..||:.          ..|:.|:  |||:|||.|..|.|.:|.
Mouse     7 VDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPT 71

  Fly   109 TFNCQAEGNPTPTIQWFKDGRELKTDTG---SHRIMLPAGGLFFLKVIHSRR-ESDAGTYWCEAK 169
            |.||:|||.|||||:|:|||..::||..   |||::||:|.||||:::|.|| :.|.|:|.|.|:
Mouse    72 TLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVAR 136

  Fly   170 NEFGVARSRNATLQVAFLRDEFRLEPANTRVAQGEVALMECGAPRGSPEPQISWRKNGQTLNLVG 234
            |..|.|.||||:|:||.|||:||..|.:..||.||.|::||..|||.|||.|.|:|:...::
Mouse   137 NYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRID--- 198

  Fly   235 NKRIRI-VDGGNLAIQEARQSDDGRYQCVVKNVVGTRESATAFLKVHVRPFLIRGPQNQTAVVGS 298
            :|..|| :.||.|.|...|:||.|.|.||..|:||.|:|..|.|.|..||..:|.|.||..:...
Mouse   199 DKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEE 263

  Fly   299 SVVFQCRIGGDPLPDVLWRRTASGGNMPLRRVHVLEDRSLKLDDVTLEDMGEYTCEADNAVGGIT 363
            :|.|:|::.|||.|.|.|::  ...::|..|..:.:|.:|::......|.|.|.|.|:|.||.:.
Mouse   264 AVEFRCQVQGDPQPTVRWKK--DDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVE 326

  Fly   364 ATGILTVH----APPKFVIRPKNQLVEIGDEVLFECQANGHPRPTLYWSVEGNSSLLLPGY-RDG 423
            |:..|||.    |||:||:||::|:|..|..|.|.|:..|:|:|.::|..||:.:||.|.. :..
Mouse   327 ASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQP 391

  Fly   424 RMEVTLTPEGRSVLSIARFAREDSGKVVTCNALNAVGSVSSRTVVSV-DTQFELPPPIIEQGPVN 487
            ....:::|.|.  |:|....|.|:|..: |.||...||:.::..:.| |...:.|||||.|||:|
Mouse   392 NSRCSVSPTGD--LTITNIQRSDAGYYI-CQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPIN 453

  Fly   488 QTLPVKSIVVLPCRTLGTPVPQVSWYLDGIPIDVQEHERRNLSDAGALTISDLQRHEDEGLYTCV 552
            |||.|....:|.|:..|.|:|.:||..:|... :....|..:.|.|.|.|.:| |..|.|.||||
Mouse   454 QTLAVDGTALLKCKATGEPLPVISWLKEGFTF-LGRDPRATIQDQGTLQIKNL-RISDTGTYTCV 516

  Fly   553 ASNRNGKSSWSGYLRLDTPTNPNIKFFRAPELSTYPGPPGKPQMVEKGENSVTLSWTRSNKVGGS 617
            |::.:|::|||..|.:   |.......:..:::..||||.|||:.:..:||||||| :....|..
Mouse   517 ATSSSGETSWSAVLDV---TESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSW-QPGTPGVL 577

  Fly   618 SLVGYVIEMFGKNETDGWVAVGTRVQNTTFTQTGLLPGVNYFFLIRAENSHGLSLPSPMSEPITV 682
            ....|:||.|.::.::.|..|...|:.|.:|..||.|...|.|::||.|..|||.|||||:|  |
Mouse   578 PASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDP--V 640

  Fly   683 GTRYFN---SGLDLSEARASLLSGD-VVELSNASVVDSTSMKLTWQI-INGKYVEGFYVYARQLP 742
            .|:..:   .|:|..:.:..|  || ||.|.|..|:..|::::||.: ...::::|:.|..||
Mouse   641 RTQDISPPAQGVDHRQVQKEL--GDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQ-- 701

  Fly   743 NPIVNNPAPVTSNTNPLLGSTSTSASASASASALISTKPNIAAAGKRDGETNQSGGGAPTPLNTK 807
                         |:.|..||     ...:..|.:.|:.:......:.|.|
Mouse   702 -------------TSGLQAST-----VWQNLDAKVPTERSAVLVNLKKGVT-------------- 734

  Fly   808 YRMLTILNGGGASSCTITGLVQYTLYEFFIVPFYKSVEGKPSNSRIARTLEDVPSEAPYGMEALL 872
                                     ||..:.|::...:|..|.|:..||.|:.||..|..:..|.
Mouse   735 -------------------------YEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLT 774

  Fly   873 L---NSSAVFLKWKAPELKDRHGVLLNYHVIVRGIDTAHNFSRILTNVTIDAASPTLVLANLTEG 934
            :   ||:::.:.|..|....::|::..|.:...|     |.:|...|.|:|||..::|:..|..|
Mouse   775 VGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLG-----NETRFHINKTVDAAIRSVVIGGLFPG 834

  Fly   935 VMYTVGVAAGNNAGVGPYCVPATLRLDPITKRLDPFINQRYPINQDHVNDVLTQPWFIILLGAIL 999
            :.|.|.|||..:||||....|..:   .|..|.:..|.:......:.:.||:.||.||..:|...
Mouse   835 IQYRVEVAASTSAGVGVKSEPQPI---IIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGAC 896

  Fly  1000 AVLMLSFGAMVFVKRKHMM-MKQSALNTMRGNH--TSDVLKMPSLSARNGNGYWLDSSTGGMVWR 1061
            .|:::.|...::.:||... :...|:...||:.  .|:..:...|:|.:.|..||
Mouse   897 WVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWL---------- 951

  Fly  1062 PSPGGDSLEMQKDHIADYAPVCGAPGSPAGGGTSSGGSGGAGSGASGGDDI---HGGHGSERNQQ 1123
                           ||..|....|.:.:..|.:..|:.|.|       |:   ..|.|.:....
Mouse   952 ---------------ADSWPATSLPVNNSNSGPNEIGNFGRG-------DVLPPVPGQGDKTATM 994

  Fly  1124 RYVGE-YSNI----PTDYAEVSSFGKAPSEYGRHGNASPAPYATSSIL---SPHQ------QQQQ 1174
            ...|. ||:|    .|.|...|...:|            .||||:.||   |.|:      ..|.
Mouse   995 LSDGAIYSSIDFTTKTTYNSSSQITQA------------TPYATTQILHSNSIHELAVDLPDPQW 1047

  Fly  1175 QQPRYQQRPVPGYGLQRPMHPHYQQQQHQQQQAQQTHQQHQALQQHQQLPPSNIYQQMSTTSEIY 1239
            :....|:..:.|:|...|       .|::...|......::..:......|.|..|..||||
Mouse  1048 KSSVQQKTDLMGFGYSLP-------DQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTS--- 1102

  Fly  1240 PTNTGPSRSVYSEQYYYPKDKQRHIHITENKLSNCHTYEAAPGAKQSSPISSQFASVRRQQLP-- 1302
             ::....||.........|.|:     |:|            .:|......|.:|:|.....|
Mouse  1103 -SHNSSERSGSLSGGKGGKKKK-----TKN------------SSKAQKNNGSTWANVPLPPPPVQ 1149

  Fly  1303 --PNCSIGRESARFKVLNTDQGKNQQNLLDLDGSSMC---------YNGLADS------------ 1344
              |...:|..:|           .|:|..|.|  |.|         :.|:.|.
Mouse  1150 PLPGTELGHYAA-----------EQENGYDSD--SWCPPLPVQTYLHQGMEDELEEDEDRVPTPP 1201

  Fly  1345 --GCGGS--------------PSP----MAMLMSHEDEHALYHTADGDLDDMERLYV-------- 1381
              |...|              |||    ..||.:|             ||::.|.|.
Mouse  1202 VRGVASSPAISFGQQSTATLTPSPREEMQPMLQAH-------------LDELTRAYQFDIAKQTW 1253

  Fly  1382 KVDEQQPPQQQQQLIPLVPQHPAEGHLQSWRNQSTRSSRKNGQECIKEPSE-------LIYAPGS 1439
            .:....||.|.    |..|.....|.|.|...........:.:|   ||.|       |...|||
Mouse  1254 HIQSNTPPPQP----PAPPLGYVSGALISDLETDVPDEDADDEE---EPLEIPRPLRALDQTPGS 1311

  Fly  1440 --------------------------VASERSLLSNSGSGT-------------SSQPAGHNVXP 1465
                                      .:|.||.:.:|..|:             ::..||..
Mouse  1312 SMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFR--- 1373

  Fly  1466 LLPGSSL---GQSQSKSQSQ--------SQSQSDLQSQEQ 1494
             |.|:||   |::.|.||.|        ..:.|..|:|.|
Mouse  1374 -LDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQ 1412

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	53/100 (53%)
		I-set	91..184	CDD:254352	53/96 (55%)
		I-set	190..279	CDD:254352	40/89 (45%)
		Ig2_Robo	193..279	CDD:143201	38/86 (44%)
		I-set	283..370	CDD:254352	28/86 (33%)
		Ig	300..370	CDD:299845	23/69 (33%)
		Ig	388..>457	CDD:299845	22/69 (32%)
		I-set	479..561	CDD:254352	34/81 (42%)
		Ig	496..561	CDD:143165	23/64 (36%)
		FN3	588..681	CDD:238020	41/92 (45%)
		FN3	<817..856	CDD:238020	6/38 (16%)
		fn3	864..952	CDD:278470	28/90 (31%)
Robo2	XP_006523098.1	Ig1_Robo	53..152	CDD:143317	53/98 (54%)
		Ig	160..244	CDD:386229	38/86 (44%)
		Ig	265..333	CDD:386229	23/69 (33%)
		Ig4_Robo	355..444	CDD:143203	27/91 (30%)
		IG	451..532	CDD:214652	33/82 (40%)
		FN3	549..642	CDD:238020	42/95 (44%)
		FN3	673..758	CDD:238020	22/143 (15%)
		FN3	767..859	CDD:238020	29/99 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6680
eggNOG	1	0.900	-	-		E1_KOG4222
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	LDO	PTHR12231
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	9	8.770