DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo2 and Robo1

DIOPT Version :9

Sequence 1:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster
Sequence 2:	XP_017172382.1	Gene:	Robo1 / 19876	MGIID:	1274781	Length:	1687	Species:	Mus musculus

Alignment Length:	1547	Identity:	429/1547 - (27%)
Similarity:	675/1547 - (43%)	Gaps:	312/1547 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    34 NKTASEIKRRHRNNRDFHGERLKIMPVDRSRRAVFPLLLLLAGLNGLTQVGALKGENPRIIEHPM 98
            |.|.:.......|:..:.|.||        |:..||                     |||:|||.
Mouse    73 NGTPAPTSDNDDNSLGYTGSRL--------RQEDFP---------------------PRIVEHPS 108

  Fly    99 DTTVPKNDPFTFNCQAEGNPTPTIQWFKDGRELKTDTG---SHRIMLPAGGLFFLKVIHSRR-ES 159
            |..|.|.:|.|.||:|||.|||||:|:|.|..::||..   |||::||:|.||||:::|.|: ..
Mouse   109 DLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRP 173

  Fly   160 DAGTYWCEAKNEFGVARSRNATLQVAFLRDEFRLEPANTRVAQGEVALMECGAPRGSPEPQISWR 224
            |.|.|.|.|:|..|.|.|.||:|:||.|||:||..|::..||.||.|:|||..|||.|||.|||:
Mouse   174 DEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWK 238

  Fly   225 KNGQTLNLVGNKRIRI-VDGGNLAIQEARQSDDGRYQCVVKNVVGTRESATAFLKVHVRPFLIRG 288
            |:|..|:   :|..|| :.||.|.|...|:||.|:|.||..|:||.|||..|.|.|..||..::.
Mouse   239 KDGSPLD---DKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKR 300

  Fly   289 PQNQTAVVGSSVVFQCRIGGDPLPDVLWRRTASGGNMPLRRVHVLEDRSLKLDDVTLEDMGEYTC 353
            |.|....|..|..|:|...|||:|.|.||:  ..|.:|..|..:.:|.:||:..||..|||.|||
Mouse   301 PSNLAVTVDDSAEFKCEARGDPVPTVRWRK--DDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTC 363

  Fly   354 EADNAVGGITATGILTVHA---PPKFVIRPKNQLVEIGDEVLFECQANGHPRPTLYWSVEGNSSL 415
            .|:|.||...|:..|||..   ||.||::|::|:|.:|..|.|:|:|.|:|:|.::|..||:.:|
Mouse   364 VAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNL 428

  Fly   416 LL---PGYRDGRMEVTLTPEGRSVLSIARFAREDSGKVVTCNALNAVGSVSSRTVVSV-DTQFEL 476
            |.   |.....|..|:.|.:    |:|....|.|.|..: |..||..||:.::..:.| |...:.
Mouse   429 LFSYQPPQSSSRFSVSQTGD----LTITNVQRSDVGYYI-CQTLNVAGSIITKAYLEVTDVIADR 488

  Fly   477 PPPIIEQGPVNQTLPVKSIVVLPCRTLGTPVPQVSWYLDGIPIDVQEHERRNLSDAGALTISDLQ 541
            |||:|.|||||||:.|...::|.|...|:|.|.:.|..||:.:..|:...:.| ::|.|.|. ..
Mouse   489 PPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQL-ESGVLQIR-YA 551

  Fly   542 RHEDEGLYTCVASNRNGKSSWSGYLRLD---------TPTNPNIKFFRAPELSTYPGPPGKPQMV 597
            :..|.|.|||.||..:|:::||.|:.:.         .||:||:          .|..|.||::.
Mouse   552 KLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNL----------IPSAPSKPEVT 606

  Fly   598 EKGENSVTLSWTRSNKVGGSSLVGYVIEMFGKNETDGWVAVGTRVQNTTFTQTGLLPGVNYFFLI 662
            :..:|:||||| :.|...|::...|:||.|.......|......|:..||...||.|...|.||:
Mouse   607 DVSKNTVTLSW-QPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLV 670

  Fly   663 RAENSHGLSLPSPMSEPI-TVGTRYFNSGLDLSEARASLLSGDVV-ELSNASVVDSTSMKLTWQI 725
            ||.|::|:|.||.:|:|: |......:.|:|..:.:..|  |:|| .|.|.:::.|:|:::.|.:
Mouse   671 RAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQREL--GNVVLHLHNPTILSSSSVEVHWTV 733

  Fly   726 -INGKYVEGFYVYARQLPNPIVNNPAPVTSNTNPLLGSTSTSASASASASALISTKPNIAAAGKR 789
             ...:|::|:.:..|                                             .:|..
Mouse   734 DQQSQYIQGYKILYR---------------------------------------------PSGAS 753

  Fly   790 DGETNQSGGGAPTPLNTKYRMLTILNGGGASSCTITGLVQYTLYEFFIVPFYKSVEGKPSNSRIA 854
            .||:........||  ||            :|..|..|.:...||....||:...:|..|..:.|
Mouse   754 HGESEWLVFEVRTP--TK------------NSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFA 804

  Fly   855 RTLEDVPSEAPYGMEALLL--NSSAVFLKWKAPELKDRHGVLLNYHVIVRGIDTAHNFSRILTNV 917
            :|||:.||..|..:.....  |.:|:.:.|:.|....::|::..|.|...|.:|.::.     |.
Mouse   805 KTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHI-----NK 864

  Fly   918 TIDAASPTLVLANLTEGVMYTVGVAAGNNAGVGPYCVPATLRLDPITKRLDPFINQRYPINQDHV 982
            |:|.::.::|:.:|..|:.|:|.|||...||.|....|..::||.....:.|  ..:..:.| .:
Mouse   865 TVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSP--EDQVSLAQ-QI 926

  Fly   983 NDVLTQPWFIILLGAILAVLMLSFGAMVFVKRKHMMMKQSALNTMRGNHTSDVLKMPSLS----- 1042
            :||:.||.||..:||...::::.|...::   :|...:....:|..|     :.|:||.:
Mouse   927 SDVVRQPAFIAGIGAACWIILMVFSIWLY---RHRKKRNGLTSTYAG-----IRKVPSFTFTPTV 983

  Fly  1043 --ARNGNGYWLDSSTGGMVWRPSPGGDSLEMQKDHIADYAPVCGAPGSPAGGGTSSGGSGGAGSG 1105
              .|.|...    |:||   ||.....|....:..:||..|..|...:.......:.|:|.:.|.
Mouse   984 TYQRGGEAV----SSGG---RPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSN 1041

  Fly  1106 ----ASGGDDIHGGHGSERNQQRYV--------GEYSNIPTDYAEVSSFGKAPSEYGRHGNAS-- 1156
                :...|.|...:....|:|..:        |:. ::.....|:.:|.....:.||..|.|
Mouse  1042 LTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDV-DLSNKINEMKTFNSPNLKDGRFVNPSGQ 1105

  Fly  1157 PAPYATSSILSPH-----------QQQQQQQPRYQQRPVPGYGLQRPMHPHYQQQQHQQQQAQQT 1210
            |.||||:.::..:           ..::..:|..||:|...     |:       |:...:..:.
Mouse  1106 PTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVA-----PI-------QYNIMEQNKL 1158

  Fly  1211 HQQHQALQQHQQLPPSNIYQQMSTTSEIYPTNTGPSRSVYSEQYYYPKDKQRHIHITENKLSNCH 1275
            ::.::|   :..:||:..|.|.      |..|||.|        |...|
Mouse  1159 NKDYRA---NDTIPPTIPYNQS------YDQNTGGS--------YNSSD---------------- 1190

  Fly  1276 TYEAAPGAKQSSPISSQFASVRRQQLPPNCSIG-RESARFKVLNTDQGKNQQNLLDLDGSSMCYN 1339
                    :.||...||               | ::.||........|.|..:||
Mouse  1191 --------RGSSTSGSQ---------------GHKKGARTPKAPKQGGMNWADLL---------- 1222

  Fly  1340 GLADSGCGGSPSPMAMLMSHEDEHALYHTADGDLDD-------MERLYVKVDEQQPPQQQQQLIP 1397
                      |.|.|....|.:......:.|...|.       ..|:|::.||.:..:.::...|
Mouse  1223 ----------PPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTP 1277

  Fly  1398 LVPQHPAEGHLQSWRNQSTRSSRKNGQECIKE-----PSELIYAPGSVASERSLLSNSGSGTSSQ 1457
            .|....:.....|:.:|||.:...:.||.::.     |.:|.:.|......|       ...|..
Mouse  1278 PVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRR-------QPVSPP 1335

  Fly  1458 PAGHNVXP-----LLPGSSLGQSQSKSQSQSQSQSDLQSQEQLYAREVRELL 1504
            |....:.|     .:.|..:....:.:..:.:.::|::..:.    :.|.||
Mouse  1336 PPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKM----QTRRLL 1383

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	50/98 (51%)
		I-set	91..184	CDD:254352	50/96 (52%)
		I-set	190..279	CDD:254352	45/89 (51%)
		Ig2_Robo	193..279	CDD:143201	43/86 (50%)
		I-set	283..370	CDD:254352	34/86 (40%)
		Ig	300..370	CDD:299845	29/69 (42%)
		Ig	388..>457	CDD:299845	23/71 (32%)
		I-set	479..561	CDD:254352	31/81 (38%)
		Ig	496..561	CDD:143165	21/64 (33%)
		FN3	588..681	CDD:238020	35/93 (38%)
		FN3	<817..856	CDD:238020	10/38 (26%)
		fn3	864..952	CDD:278470	24/89 (27%)
Robo1	XP_017172382.1	Ig1_Robo	100..199	CDD:143317	51/119 (43%)
		Ig2_Robo	207..291	CDD:143201	43/86 (50%)
		Ig3_Robo	312..380	CDD:143202	29/69 (42%)
		Ig4_Robo	401..490	CDD:143203	29/93 (31%)
		I-set	491..578	CDD:369462	34/88 (39%)
		FN3	597..683	CDD:238020	32/86 (37%)
		FN3	720..806	CDD:238020	23/144 (16%)
		fn3	814..897	CDD:365830	23/87 (26%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6680
eggNOG	1	0.900	-	-		E1_KOG4222
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	O	PTHR12231
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	9	8.770