DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo2 and Robo3

DIOPT Version :9

Sequence 1:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster
Sequence 2:	XP_006510170.1	Gene:	Robo3 / 19649	MGIID:	1343102	Length:	1403	Species:	Mus musculus

Alignment Length:	1630	Identity:	432/1630 - (26%)
Similarity:	645/1630 - (39%)	Gaps:	425/1630 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    48 RDFHGERLKIMPVDRSRRAVFPLLLLLA--GLNGLTQVGALKGENPRIIEHPMDTTVPKNDPFTF 110
            ||.......:...:.|..|:.|.||...  .||| ::||. :...|||:|.|.|..|.:.:|.|.
Mouse    21 RDISNSSELLFGFNSSLAALNPSLLPPGDPSLNG-SRVGP-EDAMPRIVEQPPDLVVSRGEPATL 83

  Fly   111 NCQAEGNPTPTIQWFKDGRELKT---DTGSHRIMLPAGGLFFLKVIHSRR-ESDAGTYWCEAKNE 171
            .|:|||.|.|.|:|:|:|..:.|   |..:||::||:|.|||.:::|.|| ..|.|.|.|.|:|.
Mouse    84 PCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRSRPDEGVYTCVARNY 148

  Fly   172 FGVARSRNATLQVAFLRDEFRLEPANTRVAQGEVALMECGAPRGSPEPQISWRKNGQTLNLVGNK 236
            .|.|.||||:|:||.|||:||..|.|..||.||.|:|||..|:|.|||.::|:|....|.   .:
Mouse   149 LGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPKGHPEPLVTWKKGKIKLK---EE 210

  Fly   237 RIRI-VDGGNLAIQEARQSDDGRYQCVVKNVVGTRESATAFLKVHVRPFLIRGPQNQTAVVGSSV 300
            ..|| :.||.|.:....:||.|.|.||..|:.|.|||..|.|.|..||..:|.|.||..:..:.|
Mouse   211 EGRITIRGGKLMMSHTFKSDAGMYMCVASNMAGERESGAAELVVLERPSFLRRPINQVVLADAPV 275

  Fly   301 VFQCRIGGDPLPDVLWRRTASGGNMPLRRVHVLEDRSLKLDDVTLEDMGEYTCEADNAVGGITAT 365
            .|.|.:.|||.|::.||:  ..|.:|..|..:..|.||.:|.|:.||.|.|||.|:|:||...|:
Mouse   276 NFLCEVQGDPQPNLHWRK--DDGELPAGRYEIRSDHSLWIDQVSSEDEGTYTCVAENSVGRAEAS 338

  Fly   366 GILTVHAPPKFVIRPKNQLVEIGDEVLFECQANGHPRPTLYWSVEGNSSLLLPGYRDGRMEVTLT 430
            |.|:||.||:||.:|:||.|..|..|.|:|:..|:|.|.::|..||:..||.|..       :|.
Mouse   339 GSLSVHVPPQFVTKPQNQTVAPGANVSFQCETKGNPPPAIFWQKEGSQVLLFPSQ-------SLQ 396

  Fly   431 PEGRSV------LSIARFAREDSGKVVTCNALNAVGSVSSRTVVSV-DTQFELPPPIIEQGPVNQ 488
            |.||.:      |:|......|.|..| |.|::..||:.::.::.: ....:..||||.|||.||
Mouse   397 PMGRLLVSPRGQLNITEVKIGDGGYYV-CQAVSVAGSILAKALLEIKGASIDGLPPIILQGPANQ 460

  Fly   489 TLPVKSIVVLPCRTLGTPVPQVSW-----YLDGIPIDVQEHERRNLSDAGALTISDLQRHEDEGL 548
            ||.:.|.|.||||.:|.|.|.:.|     :|.|      :..:.||.|.|.|.|:.:| ..|.|.
Mouse   461 TLVLGSSVWLPCRVIGNPQPNIQWKKDERWLQG------DDSQFNLMDNGTLHIASIQ-EMDMGF 518

  Fly   549 YTCVASNRNGKSSWSGYLRLDT-----------PTNPNIKFFRAPELSTYPGPPGKPQMVEKGEN 602
            |:|||.:..|:::|:.:||...           |:||             ||||.:|.:.|...|
Mouse   519 YSCVAKSSIGEATWNSWLRKQEDWGASPGPATGPSNP-------------PGPPSQPIVTEVTAN 570

  Fly   603 SVTLSWTRSNKVGGSSLVGYVIEMFGKNETDGWVAVGTRVQNTTFTQTGLLPGVNYFFLIRAENS 667
            |:||:| :.|...|::...||||.|.:...:.|..|...||..|:|.:||.|...|.||:||..:
Mouse   571 SITLTW-KPNPQSGATATSYVIEAFSQAAGNTWRTVADGVQLETYTISGLQPNTIYLFLVRAVGA 634

  Fly   668 HGLSLPSPMSEPITVGTRYFNSGLDLSEARASL-------------LSGDVVELSNASVVDSTSM 719
            .|||.|||:|||:.            ::|.:||             |:...|.:...:|:...::
Mouse   635 WGLSEPSPVSEPVQ------------TQADSSLSRPAEDPWKGQRGLAEVAVRMQEPTVLGPRTL 687

  Fly   720 KLTWQIING--KYVEGFYVYAR------------QLPNPIVNNPAPVTSNTNPLLGSTSTSASAS 770
            :::| .::|  :.|:||.|..|            .|.:|  :..:.|.....|
Mouse   688 QVSW-TVDGPVQLVQGFRVSWRIAGLDQGSWTMLDLQSP--HKQSTVLRGLPP------------ 737

  Fly   771 ASASALISTKPNIAAAGKRDGETNQSGGGAPTPLNTKYRMLTILNGGGASSCTITGLVQYTLYEF 835
               .|.|..|..:         ..|.|.||.:|..|:
Mouse   738 ---GAQIQIKVQV---------QGQEGLGAESPFVTR---------------------------- 762

  Fly   836 FIVPFYKSVEGKPSNSRIARTLEDVPSEAPYGMEALL--LNSSAVFLKWKAPELKDRHGVLLNYH 898
             .:|                  |:.||..|.|:...|  ..:|:|.:.|:.|....|:||:..|.
Mouse   763 -SIP------------------EEAPSGPPQGVAVALGGDRNSSVTVSWEPPLPSQRNGVITEYQ 808

  Fly   899 VIVRGIDTAHNFSRILTNVTIDAASPTLVLANLTEGVMYTVGVAAGNNAGVGPYCVPATLRLD-P 962
            :...|     |.||...|.:....:.::..:.|..|.:|...|||..:||||....|..::|. |
Mouse   809 IWCLG-----NESRFHLNRSAAGWARSVTFSGLLPGQIYRALVAAATSAGVGVASAPVLVQLPFP 868

  Fly   963 ITKRLDPFINQRYPINQDHVNDVLTQPWFIILLGAILAVLMLSFGAMVFVKRKHMMMKQSALNTM 1027
            ......|.:::..   .:.:..||.:|.|:....|....|:|.|.|.::.::|    ::..|:
Mouse   869 PAAEPGPEVSEGL---AERLAKVLRKPAFLAGSSAACGALLLGFCAALYRRQK----QRKELS-- 924

  Fly  1028 RGNHTSDVLKMPSLSARNGNGY-----------------WLDSSTGGMVWRPSPGGDSLEMQKDH 1075
              ::|:.....|::|..:..|.                 ||..|     |...|...|.:..:..
Mouse   925 --HYTASFAYTPAVSFPHSEGLSGSSSRPPMGLGPAAYPWLADS-----WPHPPRSPSAQEPRGS 982

  Fly  1076 IADYAPVCGAPGSPAGGGTSSGGSG--------GAGSGASG-----------GDDIHGGHGSERN 1121
                   | .|.:|.........:|        ..|:.|||           |:::...||.
Mouse   983 -------C-CPSNPDPDDRYYNEAGISLYLAQTARGANASGEGPVYSTIDPVGEELQTFHGG--- 1036

  Fly  1122 QQRYVGEYSNIPTDYAEVSSFGKAPSEY-----GRHG------------------NASPAPYATS 1163
               :....|..|:.:::.     ||.|:     |..|                  .|.|.|..:.
Mouse  1037 ---FPQHSSGDPSTWSQY-----APPEWSEGDSGARGGQGKLLGKPVQMPSLSWPEALPPPPPSC 1093

  Fly  1164 SILSPHQQQQQQQ--------------------------------PRYQQRPVPGYGLQRPMHPH 1196
            .:..|...:::.:                                ||....|...||
Mouse  1094 ELSCPEGPEEELKGSSDLEEWCPPVPEKSHLVGSSSSGACMVAPAPRDTPSPTSSYG-------- 1150

  Fly  1197 YQQQQHQQQQAQQTHQQHQALQQHQQLPPSNI--YQQMSTTSEIYPTNTGPSRSVYSEQYYYPKD 1259
                  ||..|..|.......|     ||::|  ..||.....:     |||..:...|......
Mouse  1151 ------QQSTATLTPSPPDPPQ-----PPTDIPHLHQMPRRVPL-----GPSSPLSVSQPALSSH 1199

  Fly  1260 KQRHIHITENKLSNCH--------TYEAAPG------AKQSSPISSQFASVRRQQLPPNCSIGRE 1310
            ..|.:.:....:.:.|        |..:|||      .:.:.|:....|.:|::  |......||
Mouse  1200 DGRPVGLGAGPVLSYHASPSPVPSTASSAPGRTRQVTGEMTPPLHGHRARIRKK--PKALPYRRE 1262

  Fly  1311 SARFKVLNTDQGKNQQNLLDLDGSSMCYNGLADSGCGGSPSPMAMLMSHEDEHALYHTADGDLDD 1375
            .:.                                 |..|.|.......|....|...|.|.:..
Mouse  1263 HSP---------------------------------GDLPPPPLPPPEEETSWPLGLRAAGSMSS 1294

  Fly  1376 MERLYVKVDEQQPPQQQQQLIPLVPQ--------HPAEGHLQSWRNQSTRSSRKNGQECIKEPSE 1432
            :||      |:...::..|.:||..|        ||....|                .|..|.:.
Mouse  1295 LER------ERSGERRVVQAVPLGAQPLGAQRGPHPDAALL----------------GCAAEEAW 1337

  Fly  1433 LIYAPGSVASERSLLSNSGSGTSSQPAGHNVXPLLPGSSLGQSQSKSQSQSQSQSDLQSQEQLYA 1497
            |.|...|..|.....|...:..|:...|.|       ||.|...|:...:|:|:|..|||.|...
Mouse  1338 LPYGRPSFLSHGQGTSTCSTAGSNSSRGSN-------SSRGSRGSRGPGRSRSRSRSQSQSQRPG 1395

  Fly  1498 REVRE 1502
            |..||
Mouse  1396 RNRRE 1400

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	45/98 (46%)
		I-set	91..184	CDD:254352	45/96 (47%)
		I-set	190..279	CDD:254352	38/89 (43%)
		Ig2_Robo	193..279	CDD:143201	36/86 (42%)
		I-set	283..370	CDD:254352	35/86 (41%)
		Ig	300..370	CDD:299845	30/69 (43%)
		Ig	388..>457	CDD:299845	24/74 (32%)
		I-set	479..561	CDD:254352	36/86 (42%)
		Ig	496..561	CDD:143165	25/69 (36%)
		FN3	588..681	CDD:238020	41/92 (45%)
		FN3	<817..856	CDD:238020	1/38 (3%)
		fn3	864..952	CDD:278470	26/89 (29%)
Robo3	XP_006510170.1	Ig	64..162	CDD:386229	45/97 (46%)
		Ig	170..254	CDD:386229	36/86 (42%)
		Ig	275..343	CDD:386229	30/69 (43%)
		Ig4_Robo	361..450	CDD:143203	26/96 (27%)
		Ig	451..531	CDD:386229	36/86 (42%)
		FN3	564..649	CDD:238020	36/97 (37%)
		FN3	678..761	CDD:238020	20/109 (18%)
		FN3	771..864	CDD:238020	27/97 (28%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6680
eggNOG	1	0.900	-	-		E1_KOG4222
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	O	PTHR12231
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	9	8.770