DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo2 and robo2

DIOPT Version :9

Sequence 1:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster
Sequence 2:	XP_031752453.1	Gene:	robo2 / 100493913	XenbaseID:	XB-GENE-980527	Length:	1620	Species:	Xenopus tropicalis

Alignment Length:	1595	Identity:	456/1595 - (28%)
Similarity:	690/1595 - (43%)	Gaps:	374/1595 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    57 IMPVDRSRRAVFP--LLLLLAGLNGLTQ--VGALKGEN-------------PRIIEHPMDTTVPK 104
            ||.....:||..|  ||:|:..::|:||  :...||::             |||:|||.|..|.|
Frog     5 IMLSSLRQRAASPEFLLILMLFVSGVTQSKISRTKGKHWKGGSRLRQEDFPPRIVEHPSDVIVSK 69

  Fly   105 NDPFTFNCQAEGNPTPTIQWFKDGRELKTDTG---SHRIMLPAGGLFFLKVIHSRR-ESDAGTYW 165
            .:|.|.||:|||.|||||:|:||...::||..   |||::||:|.||||::||.|: :.|.|:|.
Frog    70 GEPTTLNCKAEGRPTPTIEWYKDLERVETDKDDPRSHRMLLPSGSLFFLRIIHGRKFKPDEGSYV 134

  Fly   166 CEAKNEFGVARSRNATLQVAFLRDEFRLEPANTRVAQGEVALMECGAPRGSPEPQISWRKNGQTL 230
            |.|:|..|.|.||||:|:||.|||:|||.|.:..||.||.|::||..|||.|||.|.|:|:
Frog   135 CVARNYLGEAMSRNASLEVALLRDDFRLNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKD---- 195

  Fly   231 NLVGNKRIRIVD--------GGNLAIQEARQSDDGRYQCVVKNVVGTRESATAFLKVHVRPFLIR 287
                  ::||.|        ||.|.:...|:||.|.|.||..|:||.|:|..|.|.|..||..:|
Frog   196 ------KVRIDDKDERISIRGGKLMLSNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLR 254

  Fly   288 GPQNQTAVVGSSVVFQCRIGGDPLPDVLWRRTASGGNMPLRRVHVLEDRSLKLDDVTLEDMGEYT 352
            .|.||..:...:|.|:|::.|||.|.|.|::  ...::|..|..:.:|.:|::...:..|.|.:.
Frog   255 RPINQVVLEDEAVEFRCQVQGDPQPTVRWKK--DDVDLPRGRYDIKDDYTLRIKKASSSDEGTFA 317

  Fly   353 CEADNAVGGITATGILTVH----APPKFVIRPKNQLVEIGDEVLFECQANGHPRPTLYWSVEGNS 413
            |.::|.||.|.|:..|||.    |||:|||||::|:|..|..|.|.|:|.|:|:|.::|..||:.
Frog   318 CISENRVGKIEASATLTVRVRPVAPPQFVIRPRDQIVAQGRTVTFGCEAKGNPQPAVFWQKEGSQ 382

  Fly   414 SLLLPG---YRDGRMEVTLTPEGRSVLSIARFAREDSGKVVTCNALNAVGSVSSRTVVSV-DTQF 474
            :||.|.   ..:.|..|:.|.:    |:|....|.|:|..: |.||...||:.::..:.| |...
Frog   383 NLLFPNQPLQPNSRYSVSPTGD----LTITSIQRSDAGYYI-CQALTVAGSILAKAQLEVTDVLT 442

  Fly   475 ELPPPIIEQGPVNQTLPVKSIVVLPCRTLGTPVPQVSWYLDGIPIDVQEHERRNLSDAGALTISD 539
            :.|||||.|||.||||.|....||.|:|.|.|:|.:||..:|....:....|.::.|.|.|.|..
Frog   443 DRPPPIILQGPANQTLAVDGTAVLKCKTTGDPMPVISWLKEGFKGFIGRDARLSILDQGTLQIKG 507

  Fly   540 LQRHEDEGLYTCVASNRNGKSSWSGYLRLDTPTNPNIKFFRAPELSTYPGPPGKPQMVEKGENSV 604
            | :..|.|.|||||::.:|:::||..|.:.......|.  :..:|:..||.|.|||:.:..:|||
Frog   508 L-KVTDSGTYTCVATSSSGETTWSAVLEVTESGGAIIS--KNYDLNDLPGSPSKPQVTDVSKNSV 569

  Fly   605 TLSWTRSNKVGGSSLVGYVIEMFGKNETDGWVAVGTRVQNTTFTQTGLLPGVNYFFLIRAENSHG 669
            |||| :....|...:..|:||.|.::.::.|..|...|:.|.:|..||.|...|.|::||.|:.|
Frog   570 TLSW-QPGSPGILPVTSYIIEAFSQSVSNSWQTVANHVKTTLYTVKGLRPNTIYLFMVRAINTEG 633

  Fly   670 LSLPSPMSEPITVGTRYFN---SGLDLSEARASLLSGDV-VELSNASVVDSTSMKLTWQI-INGK 729
            ||.|||:|:|  |.|:..:   .|:|..:.:..|  ||| |.|.|..|:..|::::||.: ...:
Frog   634 LSDPSPISDP--VRTQDISPPVQGVDHRQVQKEL--GDVIVRLHNPVVLTPTTIQVTWMVDRQSQ 694

  Fly   730 YVEGFYVYARQLPNPIVNNPAPVTSNTNPLLGSTSTSASASASASALISTKPNIAAAGKRDGETN 794
            :::|:.|..||                        ||...:.||...:..|
Frog   695 FIQGYRVMYRQ------------------------TSGLQTPSAWQSLEVK-------------- 721

  Fly   795 QSGGGAPTPLNTKYRMLTILNGGGASSCTITGLVQYTLYEFFIVPFYKSVEGKPSNSRIARTLED 859
                   .|              ...|..:|.|.:...||..:.|::...:|..|.|:.|||.|:
Frog   722 -------VP--------------AERSAVLTNLKKGITYEIKVRPYFNEFQGMDSESKSARTTEE 765

  Fly   860 VPSEAPYGMEALLL---NSSAVFLKWKAPELKDRHGVLLNYHVIVRGIDTAHNFSRILTNVTIDA 921
            .||..|..:..:.:   ||:::.:.|..|....::|::.:|.:...|     |.:|...|.|:||
Frog   766 APSAPPQSVTVMTVGNNNSTSISISWDPPPSDHQNGIIQDYRIWCLG-----NETRFHINKTVDA 825

  Fly   922 ASPTLVLANLTEGVMYTVGVAAGNNAGVGPYCVPATLRLDPITKRLDPFINQRYPINQDHVNDVL 986
            |..::|:..|..|:.|.|.|||..:||.|....|..::   |..|.:..|.:......:.:.||:
Frog   826 AIRSVVIGGLFPGIQYRVEVAASTSAGTGVKSAPQHIQ---IGNRNEVVITENNNSITEQITDVV 887

  Fly   987 TQPWFIILLGAILAVLMLSFGAMVFVKRKH----------------------MMMKQSALNTMRG 1029
            .||.||..:|....|:::.|...::.:||.                      .|..:.||.|.:|
Frog   888 KQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPADSPDTTMHHKVALGTKKG 952

  Fly  1030 NHTSDVL------------KMPSLSARNGNGY-WLDSSTGGMVWRPS--PGGDSLEMQKDHIADY 1079
            :....|.            ..|.|...|...| ||..|     |..|  |..:|.....| :.::
Frog   953 SWHKTVTFQRGDGGLMSNGSRPGLLNANDPSYPWLADS-----WPASSMPVNNSNNGPND-LVNF 1011

  Fly  1080 APVCG-----APGSPAGGGTSSGGSGGAGSGASGGDDIHGGHGSERNQQRYVGEYSNIPTDYAEV 1139
            :  ||     .||.  |..|::..|.||                         .||:|  |:...
Frog  1012 S--CGDVLPTVPGQ--GEKTATMLSDGA-------------------------IYSSI--DFTTK 1045

  Fly  1140 SSFGKAPSEYGRHGNASPAPYATSSIL---SPHQ------QQQQQQPRYQQRPVPGYGLQRPMHP 1195
            ||: ..|.:.     |...||||:.||   |.|:      ..|.:....|:..:.|.|
Frog  1046 SSY-NCPGQI-----AQTTPYATTQILHSNSIHELAVDLPDPQWKSSIQQKSDLTGLG------- 1097

  Fly  1196 HYQQQQHQQQQAQQTHQQHQALQQHQQLPPSNIYQQMSTTSEIYPTNTGPSRSVYSEQYYYPKDK 1260
            :....|.:...|......::..:......|.|..|..||||....:....|.|       ..||.
Frog  1098 YCLTDQGKGSNALLYIPDYRMAEGLSNRIPHNQSQDFSTTSSHNSSERSGSLS-------GGKDG 1155

  Fly  1261 QRHIHITENKLSNCHTYEAAPGAKQSSPISSQFASVRRQQLPPNCSIGRESARFKVLNTDQGKNQ 1325
            :|    .:||.|          :|.|....|.:|:|   .|||
Frog  1156 KR----KKNKNS----------SKISKNNGSSWANV---PLPP---------------------- 1181

  Fly  1326 QNLLDLDGSSMCYNGLADSGCG----GSPSPMAMLMSHEDEHALYHTADGDLDDMERLYVKVDEQ 1386
            ..:..|.|:.:.:.||.....|    |...|:.:      :..|:..:|.:|::        ||:
Frog  1182 PPIQPLPGTEIEHYGLEHQETGYDTNGWCPPLPV------QTYLHQGSDDELEE--------DEE 1232

  Fly  1387 Q---PPQQ-----------QQQLIPLVP------QHPAEGHL-------------QSWRNQSTRS 1418
            :   ||.:           ||....|.|      |...:.||             |||...|
Frog  1233 RVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQSWHLHS--- 1294

  Fly  1419 SRKNGQECIKEPSELIYAPGSVASE------------------------RSLLSNSGSGTSSQPA 1459
               |.|..:.....|.|..|:..|:                        |.|....||...
Frog  1295 ---NNQPPLAPVPPLGYVSGTFISDLEKDIPDQEEDEIEDESIEITRPLRGLEHTPGSSMD---- 1352

  Fly  1460 GHNVXPLLPGSSL---GQSQSKSQSQSQSQSDLQS 1491
              |:...:.||.:   |.:..:.::.|..:|.:.|
Frog  1353 --NLDSSVTGSMVNGWGSASDEDRNYSSHRSSVGS 1385

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	52/111 (47%)
		I-set	91..184	CDD:254352	52/96 (54%)
		I-set	190..279	CDD:254352	40/96 (42%)
		Ig2_Robo	193..279	CDD:143201	38/93 (41%)
		I-set	283..370	CDD:254352	27/86 (31%)
		Ig	300..370	CDD:299845	22/69 (32%)
		Ig	388..>457	CDD:299845	24/71 (34%)
		I-set	479..561	CDD:254352	35/81 (43%)
		Ig	496..561	CDD:143165	24/64 (38%)
		FN3	588..681	CDD:238020	39/92 (42%)
		FN3	<817..856	CDD:238020	10/38 (26%)
		fn3	864..952	CDD:278470	26/90 (29%)
robo2	XP_031752453.1	IgC_1_Robo	56..154	CDD:409490	55/101 (54%)
		Ig strand B	73..77	CDD:409490	2/3 (67%)
		Ig strand C	86..90	CDD:409490	2/3 (67%)
		Ig strand E	113..117	CDD:409490	1/3 (33%)
		Ig strand F	132..137	CDD:409490	3/4 (75%)
		Ig strand G	146..149	CDD:409490	0/2 (0%)
		Ig	161..246	CDD:416386	36/92 (39%)
		Ig strand A	161..164	CDD:409353	2/2 (100%)
		Ig strand A'	166..170	CDD:409353	2/3 (67%)
		Ig strand B	174..181	CDD:409353	0/6 (0%)
		Ig strand C	189..194	CDD:409353	3/12 (25%)
		Ig strand C'	196..199	CDD:409353	0/2 (0%)
		Ig strand D	205..209	CDD:409353	2/3 (67%)
		Ig strand E	211..217	CDD:409353	2/5 (40%)
		Ig strand F	224..232	CDD:409353	3/7 (43%)
		Ig strand G	235..246	CDD:409353	4/10 (40%)
		Ig	253..335	CDD:416386	31/85 (36%)
		Ig strand A	253..256	CDD:409353	0/2 (0%)
		Ig strand A'	258..262	CDD:409353	2/3 (67%)
		Ig strand B	265..274	CDD:409353	0/8 (0%)
		Ig strand C	280..285	CDD:409353	1/4 (25%)
		Ig strand C'	288..291	CDD:409353	0/2 (0%)
		Ig strand D	294..299	CDD:409353	2/4 (50%)
		Ig strand E	300..307	CDD:409353	2/6 (33%)
		Ig strand F	314..322	CDD:409353	4/11 (36%)
		Ig strand G	325..335	CDD:409353	6/9 (67%)
		IgI_4_Robo	344..441	CDD:409391	37/100 (37%)
		Ig strand B	360..364	CDD:409391	0/3 (0%)
		Ig strand C	373..377	CDD:409391	1/3 (33%)
		Ig strand E	403..407	CDD:409391	2/3 (67%)
		Ig strand F	417..422	CDD:409391	2/5 (40%)
		Ig strand G	430..433	CDD:409391	1/2 (50%)
		IgI_5_Robo	448..535	CDD:409544	27/86 (31%)
		Ig strand B	464..468	CDD:409544	1/3 (33%)
		Ig strand C	477..481	CDD:409544	1/3 (33%)
		Ig strand E	501..505	CDD:409544	1/3 (33%)
		Ig strand F	515..520	CDD:409544	0/4 (0%)
		Ig strand G	528..531	CDD:409544	0/2 (0%)
		FN3	553..646	CDD:238020	34/95 (36%)
		FN3	677..762	CDD:238020	10/84 (12%)
		FN3	771..863	CDD:238020	24/94 (26%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	103	1.000	Domainoid score	I6691
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	3.000