Protein Alignment Uba2 and uba3

DIOPT Version :10

• Sequence 1: NP_524756.2
• Gene: Uba2 / 44496
• FlyBaseID: FBgn0029113
• Length: 700
• Species: Drosophila melanogaster
• Sequence 2: NP_998632.1
• Gene: uba3 / 406776
• ZFINID: ZDB-GENE-040426-2825
• Length: 462
• Species: Danio rerio
• Alignment Length: 565
• Identity: 139/565 - (24%)
• Similarity: 206/565 - (36%)
• Gaps: 219/565 - (38%)

  Fly    12 TLQELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIIDLDTIDLSNLNRQFLFHREHVGKSKAR 76
            :||.|:...|:||:||||:|||:||:|.||||..|.::|:||||:|||||||||..:.||:.||.
Zfish    61 SLQFLLDTCKILVIGAGGLGCELLKDLALSGFRHIHVVDMDTIDVSNLNRQFLFRPKDVGRPKAE 125

  Fly    77 VARESALSFNPDAKITAYHDSVTSTDYGVNFFKKFDLVLSALDNRAARNHVNRMCL--------- 132
            ||.:......|...:..:...:...|  ..|:::|.:|:..||:..||..:|.|.|
Zfish   126 VAADFVNDRVPGCSVVPHFKKIQDLD--ETFYRQFHIVVCGLDSVIARRWMNGMLLSLLIYEDGV 188

  Fly   133 ---NADVPLIESGTAGYNGQVELIKRGLTQCYECTPK--DKQRSFPGCTIRNTPSEPIHCIVWAK 192
               ::.:|||:.||.|:.|...:|..|:|.|.:||.:  ..|.:||.|||.:.|..|.||:.:.:
Zfish   189 LDPSSIIPLIDGGTEGFKGNARVILPGMTACIDCTLELYPPQINFPMCTIASMPRLPEHCVEYVR 253

  Fly   193 HLFNQLFGESLEDEDISPDAADPDAKEKDGGDGNGEPKGDGKEKGEESKEEKEAKEDTANGNIMR 257
            .|             :.|       |||..|||   ...||.:
Zfish   254 ML-------------LWP-------KEKPFGDG---VVLDGDD---------------------- 273

  Fly   258 INTRQWAKDCNYDAGKLFNKFFNEDITYLLRMSNLWKTRKAPVPVQWDTLLPEGSSGDQKDVAKQ 322
                                                     |..:||
Zfish   274 -----------------------------------------PKHIQW------------------ 279

  Fly   323 HHKVWSIEECAQVFANSLKELSANFLKLEGDDTLAWDKDDQPAMDFVAACANVRSHIFDIERKSR 387
                        |:..||                                          ||.:.
Zfish   280 ------------VYQKSL------------------------------------------ERAAE 290

  Fly   388 FEI--------KSMAGNIIPAIATTNAITAGISVMRAFKVLEAKWEQCKAVYARLRPNARNHFLV 444
            |.|        :.:...||||:|:|||:.|.......||:       ..:.|..|     |::||
Zfish   291 FNITGVTYRLTQGVVKRIIPAVASTNAVIAAACATEVFKI-------ATSAYVPL-----NNYLV 343

  Fly   445 PDASLPG---------PNPNCHVCASDPAITLKID---TKRMRIKELRDEVLVK-TLNMLNPDVT 496
            .: .:.|         ...||..|:..|.     |   |...:::|:.|.:... :|.|.:|.:|
Zfish   344 FN-DVDGLYTYTFEAERKENCSACSQVPQ-----DMQFTPSAKLQEVLDYLTENASLQMKSPAIT 402

  Fly   497 VQSNGS------ILISSEEGETECNDGKLLSELNIVDGVILKCDD 535
            ...:|.      ..::|.|..|..|..|.|.||.:|||..|...|
Zfish   403 TTLDGKNKTLYLQTVASIEERTRPNLSKTLKELGLVDGQELAVAD 447

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Uba2	NP_524756.2	Uba2_SUMO	21..459	CDD:238766	112/468 (24%)
		UAE_UbL	467..552	CDD:464286	22/79 (28%)
uba3	NP_998632.1	Interaction with ube2m N-terminus. /evidence=ECO:0000250	52..69		3/7 (43%)
		Uba3_RUB	70..367	CDD:238765	112/469 (24%)
		Interaction with ube2m N-terminus. /evidence=ECO:0000250	156..160		1/3 (33%)
		Interaction with ube2m N-terminus. /evidence=ECO:0000250	191..216		8/24 (33%)
		Interaction with nedd8. /evidence=ECO:0000250	226..228		0/1 (0%)
		Interaction with nae1. /evidence=ECO:0000250	241..247		2/5 (40%)
		Interaction with nae1. /evidence=ECO:0000250	291..294		1/2 (50%)
		Interaction with ube2m N-terminus. /evidence=ECO:0000250	330..337		1/13 (8%)
		Interaction with nedd8. /evidence=ECO:0000250	351..356		0/4 (0%)
		Interaction with ube2m core domain. /evidence=ECO:0000250	367..462		23/86 (27%)
		E2_bind	377..460	CDD:462611	20/71 (28%)