DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Uba2 and Uba3

DIOPT Version :9

Sequence 1:	NP_524756.2	Gene:	Uba2 / 44496	FlyBaseID:	FBgn0029113	Length:	700	Species:	Drosophila melanogaster
Sequence 2:	NP_035796.2	Gene:	Uba3 / 22200	MGIID:	1341217	Length:	462	Species:	Mus musculus

Alignment Length:	571	Identity:	143/571 - (25%)
Similarity:	208/571 - (36%)	Gaps:	222/571 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 FPP---TLQELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIIDLDTIDLSNLNRQFLFHREHV 70
            |.|   :||.|:...||||:||||:|||:||||.||||..|.:||:||||:|||||||||..:.|
Mouse    56 FEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDV 120

  Fly    71 GKSKARVARESALSFNPDAKITAYHDSVTSTDYGVNFFKKFDLVLSALDNRAARNHVNRMCL--- 132
            |:.||.||.|......|:..:..:.:.:  .|:...|:::|.:::..||:..||..:|.|.:
Mouse   121 GRPKAEVAAEFLNDRVPNCNVVPHFNKI--QDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLL 183

  Fly   133 ---------NADVPLIESGTAGYNGQVELIKRGLTQCYECTPK--DKQRSFPGCTIRNTPSEPIH 186
                     ::.||||:.||.|:.|...:|..|:|.|.|||.:  ..|.:||.|||.:.|..|.|
Mouse   184 NYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEH 248

  Fly   187 CIVWAKHLFNQLFGESLEDEDISPDAADPDAKEKDGGDGNGEPKGDGKEKGEESKEEKEAKEDTA 251
            ||.:.:.|
Mouse   249 CIEYVRML--------------------------------------------------------- 256

  Fly   252 NGNIMRINTRQWAKDCNYDAGKLFNKFFNEDITYLLRMSNLWKTRKAPVPVQWDTLLPEGSSGDQ 316
                      ||.|:..:..|                           ||:..|.  ||
Mouse   257 ----------QWPKEQPFGDG---------------------------VPLDGDD--PE------ 276

  Fly   317 KDVAKQHHKVWSIEECAQVFANSLKELSANFLKLEGDDTLAWDKDDQPAMDFVAACANVRSHIFD 381
                   |..|       :|..|
Mouse   277 -------HIQW-------IFQKS------------------------------------------ 285

  Fly   382 IERKSRFEIK--------SMAGNIIPAIATTNAITAGISVMRAFKVLEAKWEQCKAVYARLRPNA 438
            |||.|::.|:        .:...||||:|:|||:.|.:.....||:       ..:.|..|
Mouse   286 IERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKI-------ATSAYIPL---- 339

  Fly   439 RNHFLVPDASLPG---------PNPNCHVCASDPA-ITLKIDTKRMRI---------KELRDEVL 484
             |::||.: .:.|         ...||..|:..|. |......|...:         .:::...:
Mouse   340 -NNYLVFN-DVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAI 402

  Fly   485 VKTLNMLNPDVTVQSNGSILISSEEGETECNDGKLLSELNIVDGVILKCDD 535
            ..||...|..:.:||     ::|.|..|..|..|.|.||.:|||..|...|
Mouse   403 TATLEGKNRTLYLQS-----VTSIEERTRPNLSKTLKELGLVDGQELAVAD 448

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Uba2	NP_524756.2	ThiF	7..>177	CDD:279270	76/184 (41%)
		Uba2_SUMO	21..459	CDD:238766	116/468 (25%)
		UAE_UbL	467..552	CDD:291402	19/78 (24%)
		UBA2_C	573..691	CDD:292812
Uba3	NP_035796.2	Interaction with UBE2M N-terminus. /evidence=ECO:0000250	53..70		5/13 (38%)
		ThiF	67..366	CDD:279270	116/471 (25%)
		Uba3_RUB	71..368	CDD:238765	116/469 (25%)
		Interaction with UBE2M N-terminus. /evidence=ECO:0000250	157..161		1/3 (33%)
		Interaction with UBE2M N-terminus. /evidence=ECO:0000250	192..217		9/24 (38%)
		Interaction with NEDD8. /evidence=ECO:0000250	227..229		0/1 (0%)
		Interaction with NAE1. /evidence=ECO:0000250	242..248		2/5 (40%)
		Interaction with NAE1. /evidence=ECO:0000250	292..295		0/2 (0%)
		Interaction with UBE2M N-terminus. /evidence=ECO:0000250	331..338		1/13 (8%)
		Interaction with NEDD8. /evidence=ECO:0000250	352..357		0/4 (0%)
		Interaction with UBE2M core domain. /evidence=ECO:0000250	368..462		21/86 (24%)
		E2_bind	376..461	CDD:285976	19/78 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0476
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810