DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Shark and zap70

DIOPT Version :9

Sequence 1:	NP_524743.2	Gene:	Shark / 44353	FlyBaseID:	FBgn0015295	Length:	939	Species:	Drosophila melanogaster
Sequence 2:	NP_001018425.1	Gene:	zap70 / 553615	ZFINID:	ZDB-GENE-050522-257	Length:	604	Species:	Danio rerio

Alignment Length:	954	Identity:	218/954 - (22%)
Similarity:	334/954 - (35%)	Gaps:	387/954 - (40%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSRDSDPMKWYHGNLSREAADELLK-QGYEDGTFLVRESSTAAGDFVLSLLCQGEVCHYQVRRH- 63
            |:..:..:.:::|::||..|:|.|| .|...|.||:|:...:.|.:|||::...:..||.:.:.
Zfish     1 MTEPAADLPFFYGSISRSEAEEHLKLAGMGGGLFLLRQCLRSLGGYVLSMIWNLDFYHYSIEKQL 65

  Fly    64 -------GGEDAFFSIDDKVQTKILH-GLDTLVDYYQQAANGLPTKLTVPLIRDLPPHNTRSHGV 120
                   ||:.              | |...|.:||.:..:||...|..|.:|. |....|
Zfish    66 NGTYCIAGGKP--------------HCGPAELCEYYSKDPDGLVCTLRKPCLRS-PETEIR---- 111

  Fly   121 TNLLHRATSKNESKVVFELLKCGYRNFDAKNQDGQTALHLAALHSDEDILKHLLNAKVQVNSSDS 185
                         |.||:.|:
Zfish   112 -------------KGVFDNLR-------------------------------------------- 119

  Fly   186 FGCQPLHYAARSKPASFIRTLISAQANVQGRNIDNGYVPLHEAAKH-GNLE--AVQELLLAEAPP 247
                                             ||   .|.|..:| ..||  |:::.::::||
Zfish   120 ---------------------------------DN---MLREYVRHTWKLEGDAMEQAIISQAP- 147

  Fly   248 LPRTSSGEFPFDLAKEAGQTAVEEFLLNYKLPPANTTRDQWYHGTLTREEAVAILKKHAKELLAK 312
                       .|.|....||.|      |:|        |:||.:.|:|....|..      ..
Zfish   148 -----------QLEKLIATTAHE------KMP--------WFHGKIPRQEGERRLYS------GS 181

  Fly   313 QPEVDTSGCFLVRYSESPAASGLVLTLLCDQVVKNFRISQADLYQNGNKVQSGGSKFLYIDDGPY 377
            ||:    |.||||  |........|::...:.|.:::|.:.   ::|......|:||     ...
Zfish   182 QPD----GKFLVR--ERDEMGTFALSVTYGKTVYHYQILRD---KSGKIAMPEGTKF-----DTV 232

  Fly   378 WPSVEHLIAHFMRFSYGLPVSLKYPVPPQPKPEVPSFATI---PRSNMKPKAASPATPPTPVSPH 439
            |..||:|                     :.||:  ...|:   |..|....|.:||..|.....:
Zfish   233 WQLVEYL---------------------KMKPD--GLVTVLREPCVNQNNAAPAPANAPRRSRGN 274

  Fly   440 SHHQHPHVPALTITKKKQKENSSSMFNTLRLTSPKKALFDMNSLRKNKSKGKRSDSESSVSGSLA 504
            .:...|.||
Zfish   275 GYTPPPMVP-------------------------------------------------------- 283

  Fly   505 GTEQELQAAAPMLKSLSFSTEFSTFNADGVTGSGAAAAGEVYNVPRNNTPIEIDLPPIAQKTEAE 569
                     .||                |..||            |.:.|::.|
Zfish   284 ---------KPM----------------GAEGS------------RPSLPMDHD----------- 300

  Fly   570 VEYFTKSDVAIERERAGQWIGNGYQPTMDVLSLLDQQIKAPAVARLNSLGPNASTESEMASYLHR 634
                                  |:....|                    .||
Zfish   301 ----------------------GFTSPYD--------------------DPN------------- 310

  Fly   635 KCSGTPSTPSATEVEAAKLRFFIEPEKLVLDR-EIGHGEFGSVHSGWLLRKSGAGEESRLEVAIK 698
                          |..|...||:.:||::|. |:|.|.||.|..|....     |..:::||||
Zfish   311 --------------ELKKKTLFIKRDKLMIDEVELGSGNFGCVKKGVFKM-----ESKQIDVAIK 356

  Fly   699 MLSDEH--SNKQEFLREASVMMRLEHKCIVRLIGIAKGEMLMMVQELAPLGSMLQYILDHGHEIT 761
            :|.:|:  |.:.|.:|||.:|.:|....|||:||:.:.|.||:|.|:||.|.:.:::.....:||
Zfish   357 VLKNENEKSVRDEMMREAEIMHQLSDPFIVRMIGLCEAEALMLVMEMAPAGPLNKFLSGKKDQIT 421

  Fly   762 ANAELKVWASQIACGMHYLESQHFVHRDLAARNILLTARHQAKISDFGMSRSLRPGSTEYQFTQG 826
            .. .:.:...|::.||.|||.::||||||||||:||..:..|||||||:|::|......|:...|
Zfish   422 TE-NIVMLMHQVSMGMKYLEGRNFVHRDLAARNVLLVNQQYAKISDFGLSKALGADDNYYKARTG 485

  Fly   827 GRWPIRWYAPESFNLGIFSHASDVWSFGVTIWEMFSLGAPPYGEISNVDAIKLVDSGERLPQPNL 891
            |:||::|||||..:...||..|||||||:|:||.||.|..||.::...:.|..::.|.||..|..
Zfish   486 GKWPLKWYAPECIHFHKFSSKSDVWSFGITMWEAFSFGGKPYKKMKGPEVITYIEGGSRLDCPAA 550

  Fly   892 CPAYIYAVMQSCWKERPKDRPTFVYLTE----FF----ARDPDY 927
            ||..:|.:|:.||..:.::|..|..:.|    |:    .:.|||
Zfish   551 CPEAMYELMKECWTYKHEERLNFAKVEEKMRTFYYSIAKKIPDY 594

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Shark	NP_524743.2	SH2_Nterm_shark_like	8..91	CDD:198210	23/92 (25%)
		Ank_2	<112..184	CDD:289560	5/71 (7%)
		ANK	117..241	CDD:238125	12/126 (10%)
		ANK repeat	124..151	CDD:293786	4/26 (15%)
		ANK repeat	153..184	CDD:293786	0/30 (0%)
		Ank_2	158..247	CDD:289560	9/91 (10%)
		ANK repeat	186..217	CDD:293786	0/30 (0%)
		ANK repeat	220..245	CDD:293786	7/27 (26%)
		Ank_4	224..274	CDD:290365	13/52 (25%)
		SH2_Cterm_shark_like	287..395	CDD:198211	25/107 (23%)
		TyrKc	662..921	CDD:197581	106/265 (40%)
		PTKc_Syk_like	666..926	CDD:270650	105/270 (39%)
zap70	NP_001018425.1	SH2_N-SH2_Zap70_Syk_like	8..111	CDD:198191	31/117 (26%)
		SH2_C-SH2_Zap70	152..256	CDD:198265	34/160 (21%)
		PTKc_Zap-70	320..588	CDD:270686	108/273 (40%)
		Pkinase_Tyr	330..580	CDD:285015	104/255 (41%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	212	1.000	Domainoid score	I2722
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	262	1.000	Inparanoid score	I3085
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D345546at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_105357
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2868
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	8	7.830