Protein Alignment eIF5B and EIF5B

DIOPT Version :10

• Sequence 1: NP_651974.1
• Gene: eIF5B / 44261
• FlyBaseID: FBgn0026259
• Length: 1144
• Species: Drosophila melanogaster
• Sequence 2: NP_056988.3
• Gene: EIF5B / 9669
• HGNCID: 30793
• Length: 1220
• Species: Homo sapiens
• Alignment Length: 1256
• Identity: 632/1256 - (50%)
• Similarity: 829/1256 - (66%)
• Gaps: 148/1256 - (11%)

  Fly     1 MVKAKKGKKEILTK-----------VEGGSSVDEISDVDSDQLSLNNKQNHALKGNQAGDDDVKA 54
            |.|.:|.|.|..||           :||..:..|    ...|.|...|:....|.:...||
Human     1 MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKE----QEPQKSKGKKKKEKKKQDFDEDD---- 57

  Fly    55 VTKGVKKLNVKGKG-------KAGKNDDSDEEEVV----PAKGKASKKSAFELLMDDDEQDEPAA 108
            :.|.:::|:::.:|       .|.|..:::|||..    ..||:..||.:|    ||::.:|...
Human    58 ILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKKQSF----DDNDSEELED 118

  Fly   109 QESQSEKEEKVVVSK-------------PQKNEKKGKKAKRK--GKDDDDEDLDKVLAELQAEYA 158
            ::|:|:|..|..|..             |:|.:.|.:|:.:|  |.::|:::..|:....:...:
Human   119 KDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIKERSRINSS 183

  Fly   159 GEAAPAATTVVSPEELADEFSKKKKNKKQAKQAAVAENAGEDEASDDEEGGSTVKSAAQKKKEKK 223
            ||:.          :.:|||.:.:|.:|:.::.....|.  :..::|::....:|:.||||.|||
Human   184 GESG----------DESDEFLQSRKGQKKNQKNKPGPNI--ESGNEDDDASFKIKTVAQKKAEKK 236

  Fly   224 ERQ--KREAALA-------KQKAATEPKPKPVEKPAP---EPEPVATE-----EVQPAAEE---- 267
            ||:  ||:...|       |::..|..|.:..:|.:.   |.|.|.::     .|.||:||
Human   237 ERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQRKFEEETVKSKVTVDTGVIPASEEKAET 301

  Fly   268 ----EEEKSSKNKKKGKKDKKAEPEEEKKDAKKKGMSASMVAAMQEQLRKRKEEEERLEREEAER 328
                |::.....|||.||.||.|.||::|: ||||.|.:.|.||||.|.|.||||||.:|||.||
Human   302 PTAAEDDNEGDKKKKDKKKKKGEKEEKEKE-KKKGPSKATVKAMQEALAKLKEEEERQKREEEER 365

  Fly   329 IRLEDEREEARLEAVRLEAERKEKKKQREAERKARLKAEGKLLTKKQKEDRARAQALLESLKAQG 393
            |:..:|.|..|.|..|||.|::|:|||:|.|||.|||.|||||||.|:|.||||:|.|:.|:|||
Human   366 IKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQG 430

  Fly   394 LEIPDPND---KRPTRPGTRIR----------------------------PNKKKGPKEEAAEEE 427
            :|:|..:.   |||.....:.:                            |.|::.|.....|||
Human   431 VEVPSKDSLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEEE 495

  Fly   428 AKAAEAEAQSAAAAAAAAAAEKAEEER----VKESWDATDSEAEPEPEEETSQATNNGKTEAAEE 488
            ....:|......|.|:....||.|..:    |||:.:..:.|.|.|.|:|.|:     :.|..|.
Human   496 EDTEDAGLDDWEAMASDEETEKVEGNKVHIEVKENPEEEEEEEEEEEEDEESE-----EEEEEEG 555

  Fly   489 ENEDTDDDEDDD--DDEDD-------------SDDSDGDSDDSREVSVLANDPESRRLRAEARIL 538
            |:|.::.||:|:  .||.|             |.||:.||||.|.....|.|      :|:.||.
Human   556 ESEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAYD------KAKRRIE 614

  Fly   539 KRQAEAEKKRSTDELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIK 603
            ||:.|..|..:|::|||.::||||||||||||||||||.|||||.|||||||||||||||:|||.
Human   615 KRRLEHSKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAIN 679

  Fly   604 EQTKYVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLL 668
            ||||.:|.......|:||:|||||||||||||||||||||||||||||||||||||||||||.||
Human   680 EQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLL 744

  Fly   669 KKKKCPFIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFY 733
            |.|||||||||||||||||||:....||...||:|:.||:.||::|.|.:|::||:|||||||||
Human   745 KSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFY 809

  Fly   734 ENTDPKTYISLVPTSAISGEGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTID 798
            ||.||:|::|||||||.:|:|||:|::::.:..|.||:|||.:.|||:|.|:|||||||:|||||
Human   810 ENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTID 874

  Fly   799 AILINGKLREGQTMVVAGTDGPIVTQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLE 863
            .|||||:|:||.|::|.|.:||||||||.||:|.|||||||||.|.::|||:|||||||..||||
Human   875 VILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLE 939

  Fly   864 KAIAGINLLIAHKPDEVEICTEEVARELKSALSHIKLAQTGVHVQASTLGSLEALLEFLRTSKIP 928
            |.:||:.||:|:|.||:.:..:|:..|||..|:.|||.:.||:||||||||||||||||:||::|
Human   940 KTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVP 1004

  Fly   929 YSAIRIGPVVKRDVMKASTMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKF 993
            |:.|.||||.|:||||||.||||:.|||.||||||:|||:||||||||||:||.|:|||||||.|
Human  1005 YAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAF 1069

  Fly   994 TAYREELKQKKREEFRSVAVFPCKLRILPQFVFNSRDPIVMGVMVENGIVKVGTPICVPSKEFVD 1058
            |.||::.|::|:|||:.:||||||::||||::|||||||||||.||.|.||.|||:|||||.|||
Human  1070 TKYRQDYKKQKQEEFKHIAVFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVD 1134

  Fly  1059 IGIVTSIESNHKQIEFARKGQEICVKIDPIPGESPKMFGRHFEADDMLISKISRQSIDACKDYFR 1123
            ||||||||.||||::.|:||||:||||:||||||||||||||||.|:|:||||||||||.||:||
Human  1135 IGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFR 1199

  Fly  1124 DDLIKADWALMVELKKLFEIL 1144
            |::.|:||.|:|||||:|||:
Human  1200 DEMQKSDWQLIVELKKVFEII 1220

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_651974.1	PRK04004	553..1129	CDD:457675	413/575 (72%)
EIF5B	NP_056988.3	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..417		143/440 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	430..608		47/188 (25%)
		PRK04004	627..1202	CDD:457675	413/574 (72%)
		G1. /evidence=ECO:0000255|PROSITE-ProRule:PRU01059	638..645		6/6 (100%)
		G2. /evidence=ECO:0000255|PROSITE-ProRule:PRU01059	663..667		3/3 (100%)
		G3. /evidence=ECO:0000255|PROSITE-ProRule:PRU01059	702..705		2/2 (100%)
		G4. /evidence=ECO:0000255|PROSITE-ProRule:PRU01059	756..759		2/2 (100%)
		G5. /evidence=ECO:0000255|PROSITE-ProRule:PRU01059	824..826		1/1 (100%)