Protein Alignment eIF5B and AT1G76820

DIOPT Version :9

• Sequence 1: NP_001246599.1
• Gene: eIF5B / 44261
• FlyBaseID: FBgn0026259
• Length: 1144
• Species: Drosophila melanogaster
• Sequence 2: NP_565140.5
• Gene: AT1G76820 / 844016
• AraportID: AT1G76820
• Length: 1166
• Species: Arabidopsis thaliana
• Alignment Length: 1253
• Identity: 504/1253 - (40%)
• Similarity: 702/1253 - (56%)
• Gaps: 205/1253 - (16%)

  Fly     7 GKKE-----------ILTKVEGGSSVDEISDVDSDQLSLNNKQNHALKGNQAGDDDVKAVTKGVK 60
            |:||           :.:.:.|..|..:...:|.|:.|:..::...        ::.|.|..|.|
plant     2 GRKEPSSRGGDDEQPLASSLVGSKSDKKSVQIDDDEYSICLEEPKV--------EENKVVITGKK 58

  Fly    61 KLNVKG-KGKAGKNDDSD--------EEEVVP----------AKGKASKKS-AFELLMDDDEQDE 105
            |...:. ||...:.||.|        ||||||          :|||....| :|.||.|:|.::|
plant    59 KGKKRNKKGTQQQQDDDDDFADKFAVEEEVVPDNAFVGNNKKSKGKKDGGSVSFALLADEDNREE 123

  Fly   106 P-------AAQESQSEKEEKVVV--------SKPQKNEKK---------GKKAKRKGKD----DD 142
            .       :.::..|:|:...|:        |:.:.::.|         .||.|:|.|.    .:
plant   124 AEDNDITFSGRKKSSKKKSSSVLASIGDEESSQSKTSDSKSVELVESARSKKHKKKNKSGRTLQE 188

  Fly   143 DEDLDKVLAELQAEYAGEAAPAATTVVSPEELADEFSKKKKNKKQAKQAAVAENAGEDEASDDEE 207
            |:||||:||||     || .|||....|.||         |::.|.:..|..||.| :   .:||
plant   189 DDDLDKLLAEL-----GE-TPAAGKPASEEE---------KDQAQPEPVAPVENTGXE---GEEE 235

  Fly   208 GGSTVKSAAQKKKEKKERQKREAALAKQKAATEPKPKPVEKPAPEPEPVATEEVQPAAEEEEEKS 272
               ||::||.|||:||:.:::|...|...|||    ..||....:.|...||.:||
plant   236 ---TVETAAAKKKKKKKEKEKEKKAAAAAAAT----SSVEAKEEKQEESVTEPLQP--------- 284

  Fly   273 SKNKKKGKKDKKAEPEEEKKDAKKKGMSASMVAAMQEQLRKRKEEEERLEREEAERIRLEDEREE 337
               :||..|.|.|    |||..|.       |..:||.|.:|:|.:||.::||.|::|.|:| |.
plant   285 ---RKKDAKGKAA----EKKIPKH-------VREIQEALARRQEAKERKKKEEEEKLRKEEE-ER 334

  Fly   338 ARLEAVRLEAER-KEKKKQREAERKARLKAEGKLLTKKQKEDRARAQALLESLKAQ--GLEIPDP 399
            .|.|.:..:||. |.|:|::|.|:..|.|.||||||.|||.:..:.:|....|.|.  ||.:.|.
plant   335 RRQEELDAQAEEAKRKRKEKEKEKLLRKKLEGKLLTAKQKSEAQKREAFKNQLLAAGGGLPVADD 399

  Fly   400 ND-----KRPTRPGTRIRPNKKKGPKEEA------AEEEAKAAEAEAQSAAAAAAAAAAEKAEEE 453
            :|     |||      |..||||..:::.      .|.|.:..|..|.....:|.....:..|..
plant   400 DDDATSSKRP------IYANKKKSSRQKGNDTSVQVEGEVEPKENHALGEVGSADTEKVDLLESA 458

  Fly   454 RVKESWDATDSEAEPEPEEETSQATNNGKTEAAEEENEDTDDDEDDDDDEDDSDDSD----GDSD 514
            .:.|.                    :.|..:.|:|...    :|||:.|....|:.|    ||.|
plant   459 NLGEK--------------------SGGPADVAQENGV----EEDDEWDAKSWDNVDLKIRGDFD 499

  Fly   515 D---------SREVSVLANDPESRRLRA--------EARILKRQAEAE-----KKRST------- 550
            |         .:|.....:|.|:.:..|        |...:|..:|.|     .||:.
plant   500 DKEEEAQHVVKKEFKAHYSDHETEKPTAKPAGMSKLETAAVKAISEVEDAATQTKRAKKGKCLAP 564

  Fly   551 --------DELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTK 607
                    ::||:.:.|::||||:||||:||.:|.|:||:.||||||||||||..|.:.|:|:|:
plant   565 NEFIEEGGEKLRSIICCIMGHVDSGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAKNIRERTR 629

  Fly   608 YVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLLKKKK 672
            .:||.|  :.::||||:|||||||||:|||:|||||||:|||||||.|||:||||||:.||:.:.
plant   630 ELKADA--KLKVPGLLVIDTPGHESFTNLRSRGSSLCDLAILVVDITHGLQPQTIESLNLLRMRN 692

  Fly   673 CPFIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFYENTD 737
            ..||:||||:||||.||......:...:|:|..:...||..|.|.:|.:|.|||||..|:|:|.|
plant   693 TEFIIALNKVDRLYGWKTCKNAPIVKAMKQQNKDVINEFNLRLKKIINEFQEQGLNTELYYKNKD 757

  Fly   738 PKTYISLVPTSAISGEGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTIDAILI 802
            .....|:||||||||||:.:||..:..:.|..:.::|.|.:|:|.||||||.:.|.|||||.:|:
plant   758 MGETFSIVPTSAISGEGVPDLLLWLVQWAQKTMVEKLTYVDEVQCTVLEVKVIEGHGTTIDVVLV 822

  Fly   803 NGKLREGQTMVVAGTDGPIVTQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLEKAIA 867
            ||:|.||..:||.|..|||||.||:||.|.||||||||..|:.:||:|||||:||.|:.||.|||
plant   823 NGELHEGDQIVVCGLQGPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITAQGLEHAIA 887

  Fly   868 GINLLIAHKPDEVEICTEEVARELKSALSHIKLAQTGVHVQASTLGSLEALLEFLRTS--KIPYS 930
            |.:|.:....|::|...|....:::|.||.|..:..||:||.|||||||||||||:|.  .||.|
plant   888 GTSLHVVGPDDDIEAMKESAMEDMESVLSRIDKSGEGVYVQTSTLGSLEALLEFLKTPAVNIPVS 952

  Fly   931 AIRIGPVVKRDVMKASTMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKFTA 995
            .|.||||.|:|:|||..|||.:.:||||||||||:..||:|:||.:|||||.|||||.||::|..
plant   953 GIGIGPVHKKDIMKAGVMLEKKKEYATILAFDVKVTTEARELADEMGVKIFCADIIYQLFNQFQV 1017

  Fly   996 YREELKQKKREEFRSVAVFPCKLRILPQFVFNSRDPIVMGVMVENGIVKVGTPICVPSKEFVDIG 1060
            |.|.:|::|::|....|||||.|:|||..|||.||||::||.|.:||:|:|||||||.:||.|||
plant  1018 YIENIKEEKKKESAGEAVFPCVLQILPNCVFNKRDPIILGVKVHDGILKIGTPICVPGREFTDIG 1082

  Fly  1061 IVTSIESNHKQIEFARKGQEICVKIDPIPGESPKMFGRHFEADDMLISKISRQSIDACKDYFRDD 1125
            .:.|||:|||.:::|.||.|:.:||.....|..|||||||:.:|.|:|.|||:|||..|..:..:
plant  1083 RIASIENNHKPVDYAEKGDEVAIKIVASNREEQKMFGRHFDMEDELVSHISRRSIDILKADYMKE 1147

  Fly  1126 LIKADWALMVELKKLFEI 1143
            :....|.|:::||::|:|
plant  1148 MSTEKWKLLLKLKRIFKI 1165

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_001246599.1	PRK04004	553..1129	CDD:235195	319/577 (55%)
		IF2_eIF5B	557..769	CDD:206674	118/211 (56%)
		aeIF5B_II	779..889	CDD:293904	62/109 (57%)
		IF-2	890..993	CDD:288813	65/104 (63%)
		IF2_aeIF5B_IV	1016..1110	CDD:293911	53/93 (57%)
AT1G76820	NP_565140.5	None

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	246	1.000	Domainoid score	I543
eggNOG	1	0.900	-	-		E1_COG0532
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	818	1.000	Inparanoid score	I80
OMA	1	1.010	-	-		QHG53766
OrthoDB	1	1.010	-	-		D1124591at2759
OrthoFinder	1	1.000	-	-		FOG0002309
OrthoInspector	1	1.000	-	-		otm2596
orthoMCL	1	0.900	-	-		OOG6_101241
Panther	1	1.100	-	-	O	PTHR43381
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1735
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	14	13.840