DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eIF5B and AT1G76810

DIOPT Version :9

Sequence 1:	NP_001246599.1	Gene:	eIF5B / 44261	FlyBaseID:	FBgn0026259	Length:	1144	Species:	Drosophila melanogaster
Sequence 2:	NP_177807.3	Gene:	AT1G76810 / 844015	AraportID:	AT1G76810	Length:	1294	Species:	Arabidopsis thaliana

Alignment Length:	1309	Identity:	529/1309 - (40%)
Similarity:	726/1309 - (55%)	Gaps:	203/1309 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MVKAKKGKKEILTKVEGGSSVD-----------EISDVDSDQLSLNNKQNHALKGNQAG-----D 49
            :|.|.|.||:       |:.:|           |.|.|:.:::.:...:....|||:.|     |
plant    22 LVGATKSKKK-------GAQIDDDEYSIGTELSEESKVEEEKVVVITGKKKGKKGNKKGTQQDDD 79

  Fly    50 DDV--KAVTKGVKKLNV---------KGKGKAG--------------KND-----DSDEEEVVPA 84
            ||.  |....|||. :|         |.|||.|              |.|     |.|:|.|:..
plant    80 DDFSDKVSAAGVKD-DVPEIAFVGKKKSKGKKGGGSVSFALLDDEDEKEDNESDGDKDDEPVISF 143

  Fly    85 KGK--ASKK----------SAFELLMDDDEQDEPAAQESQSEKEEKVVVSKPQKNEKKGKKAKRK 137
            .||  ||||          |||:.|..||:..|   :..:.|:||..:....:|  ||..|:.:|
plant   144 TGKKHASKKGKKGGNSFAASAFDALGSDDDDTE---EVHEDEEEESPITFSGKK--KKSSKSSKK 203

  Fly   138 GKDDDDEDLDKVLAELQAEYAGEAAPAATTVVSPEELADEFSKKKKNKKQAKQAAVAENAGEDEA 202
            ..:....||   |.|.:...|..:.....|:...|.....||.|||:.|: |..:|..:.|:|..
plant   204 NTNSFTADL---LDEEEGTDASNSRDDENTIEDEESPEVTFSGKKKSSKK-KGGSVLASVGDDSV 264

  Fly   203 SDDEEGGST-----VKSAAQKKKEKKERQKRE-----------AALAKQKAATEP-KPKPVEKPA 250
            :|:.:...|     |::...|||:|..:..|.           |||.:..||..| ...|||:.|
plant   265 ADETKTSDTKNVEVVETGKSKKKKKNNKSGRTVQEEEDLDKLLAALGETPAAERPASSTPVEEKA 329

  Fly   251 PEPEPVATEEVQPAAEEEEEKSSKNKKKGKKDK------------------KAEPEEE------- 290
            .:|||||..|.....|.|||.::..|||.||:|                  |.|.:||
plant   330 AQPEPVAPVENAGEKEGEEETAAAKKKKKKKEKEKEKKAAAAAAATSSVEVKEEKQEESVTEPLQ 394

  Fly   291 --KKDAKKKGMSASM---VAAMQEQLRKRKEEEERLEREEAERIRLEDEREEARLEAVRLEAER- 349
              |||||.|.....:   |..|||.|.:|:|.|||.::||.|::|.|:| |..|.|.:..:||.
plant   395 PKKKDAKGKAAEKKIPKHVREMQEALARRQEAEERKKKEEEEKLRKEEE-ERRRQEELEAQAEEA 458

  Fly   350 KEKKKQREAERKARLKAEGKLLTKKQKEDRARAQALLESLKAQ--GLEIPDPNDKRPTRPGTRIR 412
            |.|:|::|.|:..|.|.||||||.|||.:..:.:|....|.|.  ||.:.| ||...|.....|.
plant   459 KRKRKEKEKEKLLRKKLEGKLLTAKQKTEAQKREAFKNQLLAAGGGLPVAD-NDGDATSSKRPIY 522

  Fly   413 PNKKKGPKEEA------AEEEAKAAEAEAQSAAAAAAAAAAEKAEEERVKESWDATDSEAEPEPE 471
            .||||..:::.      .|:|.:..|.:|............:..:.:.:
plant   523 ANKKKSSRQKGIDTSVQGEDEVEPKENQADEQDTLGEVGLTDTGKVDLI---------------- 571

  Fly   472 EETSQATNNGKTEAAEEENEDTDDDEDD-----------------DDDEDDSD------------ 507
            |..:...|:|..:.|:|...:.||:||:                 ||:|:::.
plant   572 ELVNTDENSGPADVAQENGVEEDDEEDEWDAKSWGTVDLNLKGDFDDEEEEAQPVVKKELKDAIS 636

  Fly   508 ---DSDGDSDD------------------SREVSVLANDPESRRLRAEARILKRQAEAEKKRSTD 551
               ||:.:::.                  :.||........:.|.:..::..|..|.:|.....:
plant   637 KAHDSEPEAEKPTAKPAGTGKPLIAAVKATPEVEDATRTKRATRAKDASKKGKGLAPSESIEGEE 701

  Fly   552 ELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTKYVKAAAGFE 616
            .||:.:.|::|||||||||:||.:|.|:||:.||||||||||||..|.|.|:|:||.:||.|  :
plant   702 NLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTKELKADA--K 764

  Fly   617 HRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLLKKKKCPFIVALNK 681
            .::||||:|||||||||:|||:|||||||:||||||||||||||||||:.||:.:...|||||||
plant   765 LKVPGLLVIDTPGHESFTNLRSRGSSLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNK 829

  Fly   682 IDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFYENTDPKTYISLVP 746
            :||||.||......:...:|:|..:...||..|.|::|.:|.|||||..|:|:|.|.....|:||
plant   830 VDRLYGWKTCKNAPIVKAMKQQNKDVINEFNLRLKNIINEFQEQGLNTELYYKNKDMGDTFSIVP 894

  Fly   747 TSAISGEGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTIDAILINGKLREGQT 811
            ||||||||:.:||..:..:.|..:.::|.|.:|:|.||||||.:.|.|||||.:|:||:|.||..
plant   895 TSAISGEGVPDLLLWLVQWAQKTMVEKLTYVDEVQCTVLEVKVIEGHGTTIDVVLVNGELHEGDQ 959

  Fly   812 MVVAGTDGPIVTQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLEKAIAGINLLIAHK 876
            :||.|..|||||.||:||.|.||||||||..|:.|||:|||||:||.|:.||.||||..|.:...
plant   960 IVVCGLQGPIVTTIRALLTPHPMKELRVKGTYLHYKEIKAAQGIKITAQGLEHAIAGTALHVVGP 1024

  Fly   877 PDEVEICTEEVARELKSALSHIKLAQTGVHVQASTLGSLEALLEFLRTS--KIPYSAIRIGPVVK 939
            .|::|...|....:::|.||.|..:..||:||||||||||||||:|::.  |||.|.|.||||.|
plant  1025 DDDIEAIKESAMEDMESVLSRIDKSGEGVYVQASTLGSLEALLEYLKSPAVKIPVSGIGIGPVHK 1089

  Fly   940 RDVMKASTMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKFTAYREELKQKK 1004
            :|||||..|||.:.:||||||||||:..||:|:||.:|||||.|||||||||.|.||.|.:|::|
plant  1090 KDVMKAGVMLERKKEYATILAFDVKVTTEARELADEMGVKIFCADIIYHLFDLFKAYIENIKEEK 1154

  Fly  1005 REEFRSVAVFPCKLRILPQFVFNSRDPIVMGVMVENGIVKVGTPICVPSKEFVDIGIVTSIESNH 1069
            ::|....|||||.|:|||..|||.:||||:||.|..||:|:|||||||.:||:|||.:.|||:||
plant  1155 KKESADEAVFPCVLQILPNCVFNKKDPIVLGVDVIEGILKIGTPICVPGREFIDIGRIASIENNH 1219

  Fly  1070 KQIEFARKGQEICVKIDPIPGESPKMFGRHFEADDMLISKISRQSIDACKDYFRDDLIKADWALM 1134
            |.:::|:||.::.:||.....|..|||||||:.:|.|:|.|||:|||..|..:||:|...:|.|:
plant  1220 KPVDYAKKGNKVAIKIVGSNAEEQKMFGRHFDMEDELVSHISRRSIDILKSNYRDELSLEEWKLV 1284

  Fly  1135 VELKKLFEI 1143
            |:||.:|:|
plant  1285 VKLKNIFKI 1293

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_001246599.1	PRK04004	553..1129	CDD:235195	332/577 (58%)
		IF2_eIF5B	557..769	CDD:206674	124/211 (59%)
		aeIF5B_II	779..889	CDD:293904	63/109 (58%)
		IF-2	890..993	CDD:288813	68/104 (65%)
		IF2_aeIF5B_IV	1016..1110	CDD:293911	52/93 (56%)
AT1G76810	NP_177807.3	PRK04004	700..1294	CDD:235195	339/596 (57%)
		IF2_eIF5B	706..916	CDD:206674	123/211 (58%)
		aeIF5B_II	927..1037	CDD:293904	63/109 (58%)
		IF-2	1040..1141	CDD:288813	66/100 (66%)
		IF2_aeIF5B_IV	1166..1260	CDD:293911	52/93 (56%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	246	1.000	Domainoid score	I543
eggNOG	1	0.900	-	-		E1_COG0532
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	818	1.000	Inparanoid score	I80
OMA	1	1.010	-	-		QHG53766
OrthoDB	1	1.010	-	-		D1124591at2759
OrthoFinder	1	1.000	-	-		FOG0002309
OrthoInspector	1	1.000	-	-		otm2596
orthoMCL	1	0.900	-	-		OOG6_101241
Panther	1	1.100	-	-	LDO	PTHR43381
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1735
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	14	13.840