DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment eIF5B and AT2G27700

DIOPT Version :9

Sequence 1:	NP_001246599.1	Gene:	eIF5B / 44261	FlyBaseID:	FBgn0026259	Length:	1144	Species:	Drosophila melanogaster
Sequence 2:	NP_001118399.1	Gene:	AT2G27700 / 817316	AraportID:	AT2G27700	Length:	480	Species:	Arabidopsis thaliana

Alignment Length:	487	Identity:	230/487 - (47%)
Similarity:	320/487 - (65%)	Gaps:	24/487 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly   514 DDSREVSVLANDPESRRLRAEARILKRQAEAEKKRSTDELRAGVVCVLGHVDTGKTKILDKLRRT 578
            ||.:::   ..|..|||              .|....|::|:.:.|::||:||||||:||.: ..
plant    12 DDKQQI---VGDEHSRR--------------NKLSEEDKVRSPICCMMGHIDTGKTKLLDYI-TG 58

  Fly   579 HVQDSEAGGITQQIGATNVPIEAIKEQTKYVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSL 643
            :||:.||||.||::|||.:....|.|:|..:|:..  :.::|..|.|||||:|.::|||:||..|
plant    59 NVQEGEAGGTTQKMGATYLSARNILEKTMELKSDT--KLKVPRPLFIDTPGYEFYTNLRSRGLGL 121

  Fly   644 CDIAILVVDIMHGLEPQTIESIQLLKKKKCPFIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQ 708
            ||.|||||||||||||||||.:.||:.|...||:||||:||||.|:......:.:.:|:|..:..
plant   122 CDFAILVVDIMHGLEPQTIECLNLLRMKNTEFIIALNKVDRLYGWRICENAPILEAMKQQTKDVI 186

  Fly   709 LEFQQRTKDVILQFAEQGLNAALFYENTDPKTYISLVPTSAISGEGMGNLLFMIADFCQ-NMLAK 772
            .||..|..::|.||.|||||:.|:|:|.:.....|:|||.||||||:.:||.::....| .|:.|
plant   187 NEFNMRLNEIINQFKEQGLNSELYYKNKEMGETFSIVPTCAISGEGIPDLLLLLVQLTQKTMVEK 251

  Fly   773 RLMYSEEL-QATVLEVKALPGLGTTIDAILINGKLREGQTMVVAGTDGPIVTQIRSLLMPQPMKE 836
            .:.|.::: |.||||||.:.|.|||||.:|:||:|.||..:||.|..|||||.||:||.|.|:||
plant   252 LITYVDKVQQCTVLEVKVMEGYGTTIDVVLVNGELHEGGQIVVCGLQGPIVTTIRALLTPHPIKE 316

  Fly   837 LRVKNAYVEYKEVKAAQGVKIAAKDLEKAIAGINLLIAHKPDEVEICTEEVARELKSALSHIKLA 901
            |.|...:|.::.:|||:.:.|.|||||..|.|..|.:....|::|...|.|..::.|.||.|..:
plant   317 LHVNGNHVHHEVIKAAECINIIAKDLEHVIVGTALHVVGPDDDIEAIKELVMEDVNSVLSRIDKS 381

  Fly   902 QTGVHVQASTLGSLEALLEFLRTS--KIPYSAIRIGPVVKRDVMKASTMLEHEAQYATILAFDVK 964
            ..||::|||||||||||||||::.  |:|...|.||||.|:|||||..|||.:.::|||||.||:
plant   382 GEGVYIQASTLGSLEALLEFLKSPAVKLPVGGIGIGPVQKKDVMKAGVMLERKKEFATILALDVE 446

  Fly   965 IEREAQEMADSLGVKIFQADIIYHLFDKFTAY 996
            :..||:|:||.:.||||.:||:|||||::.||
plant   447 VTTEARELADEMEVKIFCSDIMYHLFDQYQAY 478

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_001246599.1	PRK04004	553..1129	CDD:235195	222/448 (50%)
		IF2_eIF5B	557..769	CDD:206674	102/212 (48%)
		aeIF5B_II	779..889	CDD:293904	54/110 (49%)
		IF-2	890..993	CDD:288813	60/104 (58%)
		IF2_aeIF5B_IV	1016..1110	CDD:293911
AT2G27700	NP_001118399.1	GTP_EFTU	35..235	CDD:278438	100/202 (50%)
		IF2_eIF5B	37..246	CDD:206674	101/211 (48%)
		aeIF5B_II	261..369	CDD:293904	54/107 (50%)
		IF-2	373..475	CDD:288813	60/101 (59%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	246	1.000	Domainoid score	I543
eggNOG	1	0.900	-	-		E1_COG0532
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53766
OrthoDB	1	1.010	-	-		D1124591at2759
OrthoFinder	1	1.000	-	-		FOG0002309
OrthoInspector	1	1.000	-	-		otm2596
orthoMCL	1	0.900	-	-		OOG6_101241
Panther	1	1.100	-	-	O	PTHR43381
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	10	9.790