DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment eIF5B and Mtif2

DIOPT Version :9

Sequence 1:	NP_001246599.1	Gene:	eIF5B / 44261	FlyBaseID:	FBgn0026259	Length:	1144	Species:	Drosophila melanogaster
Sequence 2:	NP_001269048.1	Gene:	Mtif2 / 76784	MGIID:	1924034	Length:	746	Species:	Mus musculus

Alignment Length:	656	Identity:	169/656 - (25%)
Similarity:	262/656 - (39%)	Gaps:	186/656 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly   530 RLRAEARILKRQAEAEKKRSTD----ELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQ 590
            :|:.|.  ::...:|.::..||    :.|:.||.|:||||.|||.:|||||.|.|...|.|||||
Mouse   172 KLKQER--IRENKDAVRRPGTDPALLKPRSPVVTVMGHVDHGKTTLLDKLRETQVAAMEVGGITQ 234

  Fly   591 QIGATNVPIEAIKEQTKYVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMH 655
            .|||..|.:.:.::.|                 .:|||||.:||.:|.||:.:.||.:|||....
Mouse   235 HIGAFLVSLPSGEKIT-----------------FLDTPGHAAFSAMRARGAQVTDIVVLVVAADD 282

  Fly   656 GLEPQTIESIQLLKKKKCPFIVALNKIDRLYD------WKQLARRDVRDVLKEQQSNTQ-LEFQQ 713
            |:..||:||||..|..:.|.|:|:||.|:. |      .|:|...||  |.:|...:.| :....
Mouse   283 GVMKQTVESIQHAKDAEVPIILAINKCDKT-DADPEKVKKELLAYDV--VCEEYGGDVQAVHVSA 344

  Fly   714 RTKDVILQFAEQGLNAALFYENTDPKTYISLVPTSAISGEGMGNLLFMIADFCQNMLAKRLMYSE 778
            .|.|.::..||..:..|...|       :...||..:.|
Mouse   345 LTGDNLMALAEATIALAEILE-------LKADPTGPVEG-------------------------- 376

  Fly   779 ELQATVLEVKALPGLGTTIDAILINGKLREGQTMVVAG-----------TDGPIVTQIRSLL--- 829
                ||:|.....|.|....||:..|.||:| :::|||           .:|.|:.:....:
Mouse   377 ----TVIESFTDKGRGPVTTAIIQRGTLRKG-SILVAGKSWAKVRLIFDENGKILNEAYPSMPVG 436

  Fly   830 ------MPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLEKAIAGINLLIAHKPDEVEICTEE-- 886
                  :|....|:      :|.:....|:.|....|..:|...|        .|:::|..|:
Mouse   437 IIGWRDLPSAGDEI------LEVESEPRAREVIEWRKSEQKEEKG--------KDDLKIMEEKRR 487

  Fly   887 -------VARELKSAL-----SHIKLAQ----------TGVHVQASTL---------GSLEALLE 920
                   .|||...:|     |:||..:          ..|..|::.|         ||:||:|.
Mouse   488 EHQEAHRKAREKYGSLHWKERSYIKFLERKQQRPLKPKEKVERQSNVLPIIIKGDVDGSVEAILN 552

  Fly   921 FLRTSKIPYSA----------IRIGPVVKRDVMKASTMLEHEAQYATILAFDVKIEREAQEMADS 975
            .|.|    |.|          ..:|.:.:.||..|.|.      ...|..|:|:.....|:.|..
Mouse   553 LLDT----YDASHECELELVHFGLGDISENDVTFAETF------DGVIYGFNVEAGSAIQQSAAQ 607

  Fly   976 LGVKIFQADIIYHLFDKFTAYREELKQKKREEFRSVAVFPC-KLRILPQF-VFNSRDPI-VMGVM 1037
            .||||....|||||.:..   :|||..:..   .::..:|. :..||..| |...:..| |.|..
Mouse   608 KGVKIKLHKIIYHLIEDL---QEELSSRLP---HTLEEYPIGEASILATFTVTEGKKKIPVAGCR 666

  Fly  1038 VENG---------IVKVGTPICVPS-------KEFVDI---GIVTSIESNHKQIEFARKGQEICV 1083
            |:.|         :::.|..|...|       |:.:.:   |:...:..:.:::||....|.||.
Mouse   667 VQKGQLERHKKFKLIRNGQVIWKGSLTSLKHHKDDISVIKTGMDCGLSLDEEKVEFKPGDQVICY 731

  Fly  1084 KIDPIP 1089
            :.:.:|
Mouse   732 EENKVP 737

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_001246599.1	PRK04004	553..1129	CDD:235195	164/629 (26%)
		IF2_eIF5B	557..769	CDD:206674	74/218 (34%)
		aeIF5B_II	779..889	CDD:293904	27/138 (20%)
		IF-2	890..993	CDD:288813	36/136 (26%)
		IF2_aeIF5B_IV	1016..1110	CDD:293911	20/96 (21%)
Mtif2	NP_001269048.1	InfB	195..737	CDD:223606	163/629 (26%)
		IF2_eIF5B	200..364	CDD:206674	70/183 (38%)
		IF2_mtIF2_II	373..467	CDD:293903	22/130 (17%)
		IF-2	534..625	CDD:288813	29/100 (29%)
		mtIF2_IVc	643..730	CDD:293893	17/86 (20%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0532
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1124591at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.820