DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eIF5B and MTIF2

DIOPT Version :9

Sequence 1:	NP_001246599.1	Gene:	eIF5B / 44261	FlyBaseID:	FBgn0026259	Length:	1144	Species:	Drosophila melanogaster
Sequence 2:	NP_001005369.1	Gene:	MTIF2 / 4528	HGNCID:	7441	Length:	727	Species:	Homo sapiens

Alignment Length:	771	Identity:	183/771 - (23%)
Similarity:	287/771 - (37%)	Gaps:	210/771 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly   438 AAAAAAAAAAEKAEEERVKESWDATDSEAEPE-----PEEETSQATNNGKTEAAEEENEDTDDDE 497
            ||.:.......|.||...|....:|.|:...|     ..||.::|... .|:...|...:||.|
Human    61 AALSQYRLLVTKKEEGPWKSQLSSTKSKKVVEVWIGMTIEELARAMEK-NTDYVYEALLNTDID- 123

  Fly   498 DDDDDEDDSDDSDGDSDD--SREV----------------SVLANDPESRRLRAEARILKRQAEA 544
                  .||.::|...|:  .:||                .|..|....||.:|:..:|..
Human   124 ------IDSLEADSHLDEVWIKEVITKAGMKLKWSKLKQDKVRKNKDAVRRPQADPALLTP---- 178

  Fly   545 EKKRSTDELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTKYV 609
                     |:.||.::||||.|||.:|||.|:|.|...|.|||||.|||..|.:.:.::.|
Human   179 ---------RSPVVTIMGHVDHGKTTLLDKFRKTQVAAVETGGITQHIGAFLVSLPSGEKIT--- 231

  Fly   610 KAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLLKKKKCP 674
                          .:|||||.:||.:|.||:.:.||.:|||....|:..||:||||..|..:.|
Human   232 --------------FLDTPGHAAFSAMRARGAQVTDIVVLVVAADDGVMKQTVESIQHAKDAQVP 282

  Fly   675 FIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFYENTDPK 739
            .|:|:||.|:       |..|...|.||..:          .||:.:               |..
Human   283 IILAVNKCDK-------AEADPEKVKKELLA----------YDVVCE---------------DYG 315

  Fly   740 TYISLVPTSAISGEGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTIDAILING 804
            ..:..||.||::|:.:..|........: ||..:...:..::.||:|.....|.|....||:..|
Human   316 GDVQAVPVSALTGDNLMALAEATVALAE-MLELKADPNGPVEGTVIESFTDKGRGLVTTAIIQRG 379

  Fly   805 KLREGQTMVVAGTDGPIVTQIRSLL-------------MPQPMKELR-VKNAYVEYKEVKAAQGV 855
            .||:|..:|.    |....::|.:.             ||..:...| :.:|..|..||::....
Human   380 TLRKGSVLVA----GKCWAKVRLMFDENGKTIDEAYPSMPVGITGWRDLPSAGEEILEVESEPRA 440

  Fly   856 KIAA------KDLEKAIAGINLLIAHKPDEVEICTEEVARE--------LKSALSHIKLAQ---- 902
            :...      ::.||....:.::...:.:..|  ..:.|||        .:|.|..::..:
Human   441 REVVDWRKYEQEQEKGQEDLKIIEEKRKEHKE--AHQKAREKYGHLLWKKRSILRFLERKEQIPL 503

  Fly   903 ------------TGVHVQASTLGSLEALLEFLRTSKIPYSA----------IRIGPVVKRDVMKA 945
                        ..|.::....||:||:|..:.|    |.|          ..:|.|...||..|
Human   504 KPKEKRERDSNVLSVIIKGDVDGSVEAILNIIDT----YDASHECELELVHFGVGDVSANDVNLA 564

  Fly   946 STMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKFTAYREELKQKKREEFRS 1010
            .|.      ...|..|:|......|:.|...||||....|||.|.:..   :|||..:
Human   565 ETF------DGVIYGFNVNAGNVIQQSAAKKGVKIKLHKIIYRLVEDL---QEELSSR------- 613

  Fly  1011 VAVFPCKLR--------ILPQF-VFNSRDPI-VMGVMVENGIVKVGTPICVPSKEF--------V 1057
               .||.:.        ||..| |...:..: |.|..|:.|.::       ..|:|        :
Human   614 ---LPCAVEEHPVGEASILATFSVTEGKKKVPVAGCRVQKGQLE-------KQKKFKLTRNGHVI 668

  Fly  1058 DIGIVTSIESNHKQIEFARKGQEICVKIDPIPGESPKMFGRHFEADDMLISKISRQ 1113
            ..|.:||::.:...|...:.|.:..:.:|....|        |:..|.::....:|
Human   669 WKGSLTSLKHHKDDISIVKTGMDCGLSLDEDNME--------FQVGDRIVCYEEKQ 716

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_001246599.1	PRK04004	553..1129	CDD:235195	154/633 (24%)
		IF2_eIF5B	557..769	CDD:206674	69/211 (33%)
		aeIF5B_II	779..889	CDD:293904	24/129 (19%)
		IF-2	890..993	CDD:288813	31/136 (23%)
		IF2_aeIF5B_IV	1016..1110	CDD:293911	20/111 (18%)
MTIF2	NP_001005369.1	InfB	176..718	CDD:223606	155/648 (24%)
		IF2_eIF5B	181..345	CDD:206674	69/213 (32%)
		G1. /evidence=ECO:0000250	187..194		5/6 (83%)
		G2. /evidence=ECO:0000250	212..216		3/3 (100%)
		G3. /evidence=ECO:0000250	234..237		2/2 (100%)
		G4. /evidence=ECO:0000250	288..291		2/2 (100%)
		G5. /evidence=ECO:0000250	324..326		1/1 (100%)
		IF2_mtIF2_II	354..448	CDD:293903	21/97 (22%)
		IF-2	515..606	CDD:288813	28/100 (28%)
		mtIF2_IVc	624..711	CDD:293893	19/101 (19%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0532
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1124591at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.820