Protein Alignment eIF5B and eRF3

DIOPT Version :10

• Sequence 1: NP_651974.1
• Gene: eIF5B / 44261
• FlyBaseID: FBgn0026259
• Length: 1144
• Species: Drosophila melanogaster
• Sequence 2: NP_477259.1
• Gene: eRF3 / 34658
• FlyBaseID: FBgn0020443
• Length: 619
• Species: Drosophila melanogaster
• Alignment Length: 582
• Identity: 125/582 - (21%)
• Similarity: 206/582 - (35%)
• Gaps: 181/582 - (31%)

  Fly   391 AQGLEIPDPNDKRPTRPGTRIRPNKKKGPKEEAAEEEAKAAEAEAQSAAAAAAAAAAEKAEEERV 455
            |||.....|:.:.||.......|              |..:.|...||.|||
  Fly    87 AQGAGAAAPSAQPPTSSSNSASP--------------APGSPATTPSAGAAA------------- 124

  Fly   456 KESWDATDSEAEPEPEE--ETSQATNNGKTEAAEEENEDTDDDEDDDDDEDDSDDSDGDSDDSRE 518
                      |:|.|.:  ..|....|.:|:.|:..:.|......:|::.:|::.::|::  :.:
  Fly   125 ----------ADPTPIDGLNPSDKIANNETDPADSWDVDDAVITPEDEEVEDAEFTEGEA--TPK 177

  Fly   519 VSVLANDPESRRLRAEARILKRQAEAEKKRSTDELRAGVVCVLGHVDTGKTKI------------ 571
            ||                 .|:..:.|:.||..| ...|| .:||||.||:.|
  Fly   178 VS-----------------KKKVVKVEENRSKRE-HVNVV-FIGHVDAGKSTIGGQIMSLTGMVD 223

  Fly   572 ---LDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTKYVKAA-AGFEHRLPGLLIIDTPGHES 632
               |:|..|...:.|...........||   :..:::.|.|:.. |.||.......|:|.|||:|
  Fly   224 KRTLEKYEREAREKSRESWYLSWALDTN---QEERDKGKTVEVGRAFFETDRKHFTILDAPGHKS 285

  Fly   633 FSNLRNRGSSLCDIAILVVDIMHG-LEP------QTIESIQLLKKKKCPFIVAL-NKI-DRLYDW 688
            |......|::..|:|:||:....| .|.      ||.|...|.|......:|.| ||: |...:|
  Fly   286 FVPNMIGGAAQADLAVLVISARKGEFETGFDRGGQTREHAMLAKTAGVKHLVVLVNKMDDPTVNW 350

  Fly   689 KQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQ-GLNAALFYENTDPKTYISLVPTSAISG 752
            .|....:                   .||.||.:.:: |.|         |...::.:|.|.:||
  Fly   351 DQTRYNE-------------------CKDKILPYLKKLGFN---------PAKDLTFMPCSGLSG 387

  Fly   753 EGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGL-------------------GTTID 798
            .|:           ::.:.:.|.......|.:..:..||.|                   ||.:.
  Fly   388 YGL-----------KDQIPETLCPWYRGPAFIPFIDELPSLNRKSDGPFIMPIVDKYKDMGTVVM 441

  Fly   799 AILINGKLREGQTMVVAGTDGPIVTQIR---------SLLMPQPMKELRVKNAYVEYKEVK---- 850
            ..:.:|..|:||.::|.    |..||:.         .:....|.:.:::|...:|.::|.
  Fly   442 GKVESGTARKGQNLLVM----PNRTQVAVDQLFSDDFEVTSVGPGENVKIKLKGIEEEDVSPGFV 502

  Fly   851 ---AAQGVKIAAKDLEKAIAGINLLIAHKPDEVEICTEEVARELKSALSHIKLAQTGVHVQA 909
               ||..:| ..|..:..:    :::.||.   .||..      .||:.||..|...|.|:|
  Fly   503 LCDAANPIK-TGKIFDAQV----VILEHKS---IICAG------YSAVMHIHCAAEEVTVKA 550

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_651974.1	PRK04004	553..1129	CDD:457675	93/418 (22%)
eRF3	NP_477259.1	PAM2	12..28	CDD:429316
		EF1_alpha	197..416	CDD:206670	61/261 (23%)
		eRF3_II	424..505	CDD:293906	14/84 (17%)
		eRF3_C_III	511..617	CDD:294003	14/54 (26%)