DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment eIF5B and Mtif2

DIOPT Version :9

Sequence 1:	NP_001246599.1	Gene:	eIF5B / 44261	FlyBaseID:	FBgn0026259	Length:	1144	Species:	Drosophila melanogaster
Sequence 2:	NP_001004254.1	Gene:	Mtif2 / 305606	RGDID:	1303046	Length:	727	Species:	Rattus norvegicus

Alignment Length:	844	Identity:	192/844 - (22%)
Similarity:	295/844 - (34%)	Gaps:	290/844 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly   344 RLEAERKEKKKQREAERKARLKAEGKLLTKKQKEDRARAQA-----LLESLKAQGLEIPDPNDKR 403
            ||...:|||:..|......:.|.|.::......|:.|||.|     :.|:|....::|
  Rat    67 RLLVTKKEKRPPRSQLSPIKTKKEVEVWVGMTVEELARAMAKDTDCVYEALLNTAIDI------- 124

  Fly   404 PTRPGTRIRPNKKKGPKEEAAEEEAKAAEAEAQSAAAAAAAAAA-EKAEEERVKESWDATDSEAE 467
                              ::.|..:...|...:.....|..... .|.:.||::|:.||.
  Rat   125 ------------------DSLEANSHLDEVWIKEVIKKAGMKLKWSKLKLERIRENKDAV----- 166

  Fly   468 PEPEEETSQATNNGKTEAAEEENEDTDDDEDDDDDEDDSDDSDGDSDDSREVSVLANDPESRRLR 532
                                                                         ||..
  Rat   167 -------------------------------------------------------------RRPG 170

  Fly   533 AEARILKRQAEAEKKRSTDELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNV 597
            |:..:||.             |:.||.::||||.|||.:|||||.|.|...|.|||||.|||..|
  Rat   171 ADPALLKP-------------RSPVVTIMGHVDHGKTTLLDKLRETQVAAMEVGGITQHIGAFLV 222

  Fly   598 PIEAIKEQTKYVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTI 662
            .:.:.::.|                 .:|||||.:||.:|.||:.:.||.:|||....|:..||:
  Rat   223 SLPSGEKIT-----------------FLDTPGHAAFSAMRARGAQVTDIVVLVVAADDGVMKQTV 270

  Fly   663 ESIQLLKKKKCPFIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGL 727
            ||||..|....|.|:|:||.|:       ...|...|.||..:          .||:.:  |.|.
  Rat   271 ESIQHAKDAGVPIILAINKCDK-------TDADPEKVKKELLA----------YDVVCE--EYGG 316

  Fly   728 NAALFYENTDPKTYISLVPTSAISGEGMGNLLFM----IADFCQNMLAKRLMYSEELQATVLEVK 788
            :             :..|..||::|:   ||:.:    ||  ...||..:...:..::.||:|..
  Rat   317 D-------------VQAVHVSALTGK---NLMALAEATIA--LAEMLELKADPTGPVEGTVIESF 363

  Fly   789 ALPGLGTTIDAILINGKLREGQTMVVAGTDGPIVTQIRSLLMPQPMKELRVKNAY---------- 843
            ...|.|....||:..|.|::|..:|.    |....::| |:..:..|  .|..||
  Rat   364 TDKGRGPVTTAIIQRGTLKKGSILVA----GKSWAKVR-LMFDENGK--TVNEAYPSMPVGIIGW 421

  Fly   844 ----------VEYKEVKAAQGVKIAAKDLEKAIAGINLLIAHKPDEVEICTEE---------VAR 889
                      :|.:....|:.|....|..:|...|        .|:::|..|:         .||
  Rat   422 RDLPSAGDEILEVQSEPRAREVVDWRKSEQKEEKG--------KDDLKIMEEKRKEHQEAHRKAR 478

  Fly   890 ELKSAL-----SHIKLAQ-------------------TGVHVQASTLGSLEALLEFLRTSKIPYS 930
            |...:|     |:||..:                   ..:.::....||:||:|..|.|    |.
  Rat   479 EKYGSLHWKQRSYIKYLERKEQRPLKPKEKVERDSNVLPIIIKGDVDGSVEAILNLLDT----YD 539

  Fly   931 A----------IRIGPVVKRDVMKASTMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADI 985
            |          ..:|.:.:.||..|.|.      ...|..|:|......|:.|...||||....|
  Rat   540 ASHECELELVHFGLGDISENDVTFAETF------DGVIYGFNVDAGSAIQQSAAQKGVKIKLHKI 598

  Fly   986 IYHLFDKFTAYREELKQK---KREEFRSVAVFPC-KLRILPQFVF--NSRDPIVMGVMVENG--- 1041
            ||||.:..   :|||..:   |.||      :|. :..||..|..  ..:...|.|..|:.|
  Rat   599 IYHLIEDL---QEELSNRLPYKVEE------YPIGEASILATFTITEGKKKVPVAGCRVQKGQLE 654

  Fly  1042 ------IVKVGTPICVPS-------KE---FVDIGIVTSIESNHKQIEFARKGQEICVKIDPIP 1089
                  :::.|..|...|       |:   .:..|:...:..:.:.:||....|.||.:.:.:|
  Rat   655 RQKKFKLIRNGQVIWKGSLTSLKHHKDDVLVIKTGVDCGLSLDEENVEFKVGDQVICYEENKVP 718

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_001246599.1	PRK04004	553..1129	CDD:235195	162/629 (26%)
		IF2_eIF5B	557..769	CDD:206674	72/215 (33%)
		aeIF5B_II	779..889	CDD:293904	27/138 (20%)
		IF-2	890..993	CDD:288813	33/136 (24%)
		IF2_aeIF5B_IV	1016..1110	CDD:293911	18/96 (19%)
Mtif2	NP_001004254.1	InfB	<72..720	CDD:392272	190/839 (23%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0532
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1124591at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.820