DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eIF5B and SPAC56F8.03

DIOPT Version :9

Sequence 1:	NP_001246599.1	Gene:	eIF5B / 44261	FlyBaseID:	FBgn0026259	Length:	1144	Species:	Drosophila melanogaster
Sequence 2:	NP_593218.1	Gene:	SPAC56F8.03 / 2543241	PomBaseID:	SPAC56F8.03	Length:	1079	Species:	Schizosaccharomyces pombe

Alignment Length:	1177	Identity:	481/1177 - (40%)
Similarity:	691/1177 - (58%)	Gaps:	137/1177 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 KKGKKEILTKVEGGSSVDEISDVDS--DQLSLNNKQNHAL--KGNQAGDDDVKAVTKGVKKLNVK 65
            |||||......|.... ::||..:.  |..|.::.||..|  |.........|..:|  ||...|
pombe     3 KKGKKSGYADWEDDLG-EDISGQNEYLDNTSQDSPQNDELAEKSENLAVSSEKTTSK--KKKGKK 64

  Fly    66 GKGKAGKNDDSDEEEVVPAKGKASKKSAFELLMDDDEQDEPAAQESQSEKEEKVVVSKPQKNEKK 130
            .||...:..|.:.:|:...:|.....:..||  ||||.|                 .||:|. ||
pombe    65 NKGNKNQVSDDESQELESPQGPKELTAVTEL--DDDEFD-----------------YKPKKG-KK 109

  Fly   131 GKKAKRKGKDDDDEDLDKVLAELQAEYAGEAAPAATTVVSPEEL-ADEFSKKKKNKKQAKQAAVA 194
            |||:|:..:||:.::::           ....|...|.|:  || .|||..|.|..|:.|:   |
pombe   110 GKKSKKVEEDDEPQEIE-----------SPQGPKELTAVT--ELDDDEFDYKPKKGKKGKK---A 158

  Fly   195 ENAGEDEASDDEEGGST-VKSAAQKKKEKKERQK--REAALAKQKAATEPKPKPVEKPAPEPEPV 256
            :|..|.||:...|.... ||:..:|::|||||:|  ::...||:|.:|            ..:.:
pombe   159 QNNNESEAAAPPEIPEVRVKTKKEKEREKKEREKLRKKQQQAKKKGST------------GEDTL 211

  Fly   257 ATEEVQPAAEEEEEKSSKNKKKGKKDKKAEPEEEKKDAKKKGMSASMVAAMQEQLRKRKEEEERL 321
            |:.||....:......:.:..|||         :...:|:||.:.:.:..|.|:.|.|:|||:|:
pombe   212 ASSEVSSEVDISTPAENDSSAKGK---------QAAGSKRKGPNVTALQKMLEEKRAREEEEQRI 267

  Fly   322 EREEAERIRLEDER----EEARLEAVRLEAERKEKKKQREAERKARLKAEGKLLTKKQKEDRARA 382
            ..||| ||..|::|    ||||.|..||      |||::|.::|..:||:||.|:|||||.:|.|
pombe   268 REEEA-RIAEEEKRLAEVEEARKEEARL------KKKEKERKKKEEMKAQGKYLSKKQKEQQALA 325

  Fly   383 QALLESLKAQGLEIPD-PNDKRPTRPGTRIRPNKKKGPKEEAAEEEAKAAEAEAQSAAAAAAAAA 446
            |..|:.:...|:.:.. .|.::..:|   :..||||         ..::..:...|:....::.|
pombe   326 QRRLQQMLESGVRVAGLSNGEKKQKP---VYTNKKK---------SNRSGTSSISSSGILESSPA 378

  Fly   447 AEKAEEERVKESWDATDSE-AEPEPEEETSQATNNGKTEA-------------AEEENEDTDDDE 497
            ...:.:|..|:|.|  ||| .|.|.|.|..: .|..:.||             ..|.||...:.:
pombe   379 TSISVDEPQKDSKD--DSEKVEKETEVERKE-ENEAEAEAVFDDWEAALEEPEVAENNEVVTEKK 440

  Fly   498 DDDDDEDDSDDSDGDSDDSREVSVLANDPESRRLRAEARILKRQAEAEKKRSTDELRAGVVCVLG 562
            :.|...|..:.|..|.:||:...|  :|            :.:.|.||...|..:||:.:.|:||
pombe   441 ETDIKSDAVEHSIKDKEDSKTDKV--DD------------IPQAAPAESNVSESDLRSPICCILG 491

  Fly   563 HVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTKYVKAAAGFEHRLPGLLIIDT 627
            ||||||||:||.|||::||:.||||||||||||..|||:||::||.|......::.:||||||||
pombe   492 HVDTGKTKLLDNLRRSNVQEGEAGGITQQIGATYFPIESIKQKTKVVNKKGKLQYNIPGLLIIDT 556

  Fly   628 PGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLLKKKKCPFIVALNKIDRLYDWKQLA 692
            ||||||:|||:||:|||:|||||:||||||||||||||:||:.:|.||:|||||:||||.|..:.
pombe   557 PGHESFTNLRSRGTSLCNIAILVIDIMHGLEPQTIESIRLLRDQKTPFVVALNKVDRLYGWHSIK 621

  Fly   693 RRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFYENTDPKTYISLVPTSAISGEGMGN 757
            ...::|.|.:|:...|.||:.|.:.:|||..||||||||::||.:...|:|||||||.||||:.:
pombe   622 DNAIQDSLSKQKKAIQREFRDRVESIILQLNEQGLNAALYFENKNLGRYVSLVPTSAQSGEGVPD 686

  Fly   758 LLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTIDAILINGKLREGQTMVVAGTDGPIV 822
            |:.::....|..::.|:.|...|:.||||||.:.|||.|||.||.||.|.||..:|:.|..|||:
pombe   687 LVALLISLTQTRMSDRIKYITTLECTVLEVKVIEGLGATIDVILSNGVLHEGDRIVLCGMGGPII 751

  Fly   823 TQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLEKAIAGINLLIAHKPDEVEICTEEV 887
            |.:|:||.|||:||:|||:|||.:||:|||.||||.|.|||||:||..||:....|:.|...||:
pombe   752 TTVRALLTPQPLKEMRVKSAYVHHKEIKAAMGVKICANDLEKAVAGSRLLVVGPDDDEEDLAEEI 816

  Fly   888 ARELKSALSHIKLAQTGVHVQASTLGSLEALLEFLRTSKIPYSAIRIGPVVKRDVMKASTMLEHE 952
            ..:|::.|..|..:..||.||||||||||||||||:..|||.:::.||||.|:|||:.:||||..
pombe   817 MEDLENLLGRIDTSGIGVSVQASTLGSLEALLEFLKQMKIPVASVNIGPVYKKDVMRCATMLEKA 881

  Fly   953 AQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKFTAYREELKQKKREEFRSVAVFPCK 1017
            .:||.:|.||||::|:|:::|:.||||||.|::||||||.|||:::::.::||||...||||||.
pombe   882 KEYALMLCFDVKVDRDAEDLAEQLGVKIFSANVIYHLFDAFTAHQKKILEQKREESSDVAVFPCV 946

  Fly  1018 LRILPQFVFNSRDPIVMGVMVENGIVKVGTPIC----VPSKE--FVDIGIVTSIESNHKQIEFAR 1076
            |:.:.  .||.||||::||.|..|::::.|||.    :|:.|  .:::|.|.|:|.|||.::..:
pombe   947 LKTVA--AFNKRDPIILGVDVVEGVLRINTPIVAVKQLPNGEPQIIELGRVASLEMNHKPVDKVK 1009

  Fly  1077 KGQE---ICVKIDPIPGESPKMFGRHFEADDMLISKISRQSIDACKD-YFRDDLIKADWALMVEL 1137
            |||.   :.:|::  ...|..:|||.....|.|.|.|:|||||:.|| .|||::.:.:|.|:::|
pombe  1010 KGQAGAGVAMKLE--SSGSQILFGRQVTESDALYSHITRQSIDSLKDPAFRDEVSRDEWQLIIQL 1072

  Fly  1138 KKLFEIL 1144
            ||||.|:
pombe  1073 KKLFGII 1079

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_001246599.1	PRK04004	553..1129	CDD:235195	312/585 (53%)
		IF2_eIF5B	557..769	CDD:206674	128/211 (61%)
		aeIF5B_II	779..889	CDD:293904	63/109 (58%)
		IF-2	890..993	CDD:288813	59/102 (58%)
		IF2_aeIF5B_IV	1016..1110	CDD:293911	35/102 (34%)
SPAC56F8.03	NP_593218.1	PRK04004	479..1065	CDD:235195	312/589 (53%)
		IF2_eIF5B	485..697	CDD:206674	127/211 (60%)
		aeIF5B_II	709..818	CDD:293904	63/108 (58%)
		IF-2	821..920	CDD:288813	56/98 (57%)
		Translation_Factor_II_like	953..1044	CDD:295476	33/92 (36%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	265	1.000	Domainoid score	I355
eggNOG	1	0.900	-	-		E1_COG0532
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	754	1.000	Inparanoid score	I82
OMA	1	1.010	-	-		QHG53766
OrthoFinder	1	1.000	-	-		FOG0002309
OrthoInspector	1	1.000	-	-		oto101902
orthoMCL	1	0.900	-	-		OOG6_101241
Panther	1	1.100	-	-	LDO	PTHR43381
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R593
SonicParanoid	1	1.000	-	-		X1735
TreeFam	1	0.960	-	-
	13	12.860