DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eIF5B and Eif5b

DIOPT Version :9

Sequence 1:	NP_001246599.1	Gene:	eIF5B / 44261	FlyBaseID:	FBgn0026259	Length:	1144	Species:	Drosophila melanogaster
Sequence 2:	NP_938045.2	Gene:	Eif5b / 226982	MGIID:	2441772	Length:	1216	Species:	Mus musculus

Alignment Length:	1255	Identity:	632/1255 - (50%)
Similarity:	823/1255 - (65%)	Gaps:	150/1255 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MVKAKKGKKEILTK-----------VEGGSSVDEISDVDSDQLSLNNKQNHALKGNQAGDDDVKA 54
            |.|.:|.|.|..||           :||..:..|    ...|.|...|:    |..:..|.|...
Mouse     1 MGKKQKNKSEDSTKDDTDLGALAAEIEGAGAAKE----QEPQKSKGKKK----KEKKKQDFDEND 57

  Fly    55 VTKGVKKLNVKGKG-------KAGKNDDSDEEEVV-----PAKGKASKKSAFELLMDDDEQDEPA 107
            :.:.:::|:::.:|       .|.|..:::|||..     ..||:..||::|    |:::.:|..
Mouse    58 ILRELEELSLEAQGIRADRDAAAVKPTENNEEESASKQDKKKKGQKGKKTSF----DENDSEELE 118

  Fly   108 AQESQSEKEEKVVVSKP----------QKNEKKGKKA-----KRKGKDDDDEDLDKVLAELQAEY 157
            .::|:|:|..:.....|          .|..||||||     ||.|.::|:::..:.....:...
Mouse   119 DKDSKSKKTARPNSEAPLSGSEDADDSNKLSKKGKKAQKSTKKRDGSEEDEDNSKRSKERSRVNS 183

  Fly   158 AGEAAPAATTVVSPEELADEF--SKK--KKNKKQAKQAAVAENAGEDEASDDEEGGSTVKSAAQK 218
            :||:...          :|||  |:|  |||:|......|      |..::|::....:|:.|||
Mouse   184 SGESGGE----------SDEFLQSRKGQKKNQKNKSVPTV------DSGNEDDDSSFKIKTVAQK 232

  Fly   219 KKEKKERQKR-----EAALAK-------------QKAATEPKPKPVE-----KPAPEPEPVATEE 260
            |.|||||:|:     :|.|.|             |....||:.:|.|     :..|:....:.|:
Mouse   233 KAEKKEREKKKRDEEKAKLRKMKEKEELEKGKKEQSKQREPQKRPEEEVLTLRGTPDTGAASEEK 297

  Fly   261 VQPAAEEEEEKSSKNKKKGKKDKKAEPEEEKKDAKKKGMSASMVAAMQEQLRKRKEEEERLEREE 325
            ...||..|::.....|||.||.||.|.:|::|: ||||.|.|.|.|:||.|.|.||||||.:|||
Mouse   298 GDTAAALEDDNEGDKKKKDKKKKKTEKDEKEKE-KKKGPSKSTVKAIQEALAKLKEEEERQKREE 361

  Fly   326 AERIRLEDEREEARLEAVRLEAERKEKKKQREAERKARLKAEGKLLTKKQKEDRARAQALLESLK 390
            .|||:..:|.|..|.|..|||.|::|:|||:|.|||.|||.|||||||.|:|.||||:..|..|:
Mouse   362 EERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEVTLRHLQ 426

  Fly   391 AQGLEIPDPND---KRPTRPGTRIRPNKKKGPKEEAAEEEAKAAEAEAQSAAA-------AAAAA 445
            |||:|:|..:.   |||.....:    |||.|::..::|.::..|..|...|.       .....
Mouse   427 AQGVEVPSKDSLPKKRPVYEDKK----KKKTPQQLESKEVSETLEISAPVEAVDQGGPEKEETPP 487

  Fly   446 AAEKAEEERVKES----WDATDSEAEPEPE--------EETSQATNNGKTEAAEEENEDTD---D 495
            :.|..|||..:::    |:|..|:.|.|.|        ||..:.....:.|..|||:||.:   |
Mouse   488 SVEPEEEEDTEDAGLDDWEAMASDEEREKEGNMIHIEVEENPEEEEEEEEEEEEEESEDEEEEGD 552

  Fly   496 DEDDDDDEDD---------------------SDDSDGDSDDSREVSVLANDPESRRLRAEARILK 539
            .|..|.||:|                     |.||:.||||.|.....|.|      :|:.||.|
Mouse   553 SEGSDGDEEDCKLSDEKDSGKAGDTKPSKDASSDSEYDSDDDRTKEERAYD------KAKRRIEK 611

  Fly   540 RQAEAEKKRSTDELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIKE 604
            |:.|..|..:|::|||.::||||||||||||||||||.|||||.|||||||||||||||:|||.|
Mouse   612 RRLEHGKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINE 676

  Fly   605 QTKYVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLLK 669
            |||.:|.......|:||:|||||||||||||||||||||||||||||||||||||||||||.:||
Mouse   677 QTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINILK 741

  Fly   670 KKKCPFIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFYE 734
            .|||||||||||||||||||:....||...||:|:.||:.||::|.|.:|::||:||||||||||
Mouse   742 SKKCPFIVALNKIDRLYDWKKSPDSDVAVTLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYE 806

  Fly   735 NTDPKTYISLVPTSAISGEGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTIDA 799
            |.||:|::|||||||.:|:|||:|::::.:..|.||:|||.:.|||:|.|:|||||||:|||||.
Mouse   807 NKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDV 871

  Fly   800 ILINGKLREGQTMVVAGTDGPIVTQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLEK 864
            |||||:|:||.|::|.|.:||||||||.||:|.|||||||||.|.::|||:|||||||..|||||
Mouse   872 ILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEK 936

  Fly   865 AIAGINLLIAHKPDEVEICTEEVARELKSALSHIKLAQTGVHVQASTLGSLEALLEFLRTSKIPY 929
            .:||:.||:|:|.||:.:..:|:..|||..|:.|||.:.||:||||||||||||||||:||::||
Mouse   937 TLAGLPLLVAYKDDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPY 1001

  Fly   930 SAIRIGPVVKRDVMKASTMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKFT 994
            :.|.||||.|:||||||.||||:.|||.||||||:|||:||||||||||:||.|:|||||||.||
Mouse  1002 AGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFT 1066

  Fly   995 AYREELKQKKREEFRSVAVFPCKLRILPQFVFNSRDPIVMGVMVENGIVKVGTPICVPSKEFVDI 1059
            .||::.|::|:|||:.:||||||::||||::||||||||:||.||.|.||.|||:|||||.||||
Mouse  1067 KYRQDYKKQKQEEFKHIAVFPCKMKILPQYIFNSRDPIVIGVTVEAGQVKQGTPMCVPSKNFVDI 1131

  Fly  1060 GIVTSIESNHKQIEFARKGQEICVKIDPIPGESPKMFGRHFEADDMLISKISRQSIDACKDYFRD 1124
            |||||||.||||::.|:||||:||||:||||||||||||||||.|:|:||||||||||.||:|||
Mouse  1132 GIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRD 1196

  Fly  1125 DLIKADWALMVELKKLFEIL 1144
            ::.|:||.|:|||||:|||:
Mouse  1197 EMQKSDWQLIVELKKVFEII 1216

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_001246599.1	PRK04004	553..1129	CDD:235195	411/575 (71%)
		IF2_eIF5B	557..769	CDD:206674	154/211 (73%)
		aeIF5B_II	779..889	CDD:293904	72/109 (66%)
		IF-2	890..993	CDD:288813	77/102 (75%)
		IF2_aeIF5B_IV	1016..1110	CDD:293911	72/93 (77%)
Eif5b	NP_938045.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..604		201/641 (31%)
		PRK04004	623..1198	CDD:235195	411/574 (72%)
		IF2_eIF5B	628..841	CDD:206674	154/212 (73%)
		G1. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	634..641		6/6 (100%)
		G2. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	659..663		3/3 (100%)
		G3. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	698..701		2/2 (100%)
		G4. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	752..755		2/2 (100%)
		G5. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	820..822		1/1 (100%)
		aeIF5B_II	851..961	CDD:293904	72/109 (66%)
		IF-2	962..1063	CDD:288813	75/100 (75%)
		IF2_aeIF5B_IV	1088..1182	CDD:293911	72/93 (77%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	317	1.000	Domainoid score	I1266
eggNOG	1	0.900	-	-		E1_COG0532
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1096	1.000	Inparanoid score	I238
Isobase	1	0.950	-	0	Normalized mean entropy	S977
OMA	1	1.010	-	-		QHG53766
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002309
OrthoInspector	1	1.000	-	-		oto94841
orthoMCL	1	0.900	-	-		OOG6_101241
Panther	1	1.100	-	-	LDO	PTHR43381
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R593
SonicParanoid	1	1.000	-	-		X1735
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.810