Protein Alignment eIF5B and iffb-1

DIOPT Version :9

• Sequence 1: NP_001246599.1
• Gene: eIF5B / 44261
• FlyBaseID: FBgn0026259
• Length: 1144
• Species: Drosophila melanogaster
• Sequence 2: NP_497536.2
• Gene: iffb-1 / 175353
• WormBaseID: WBGene00021857
• Length: 1074
• Species: Caenorhabditis elegans
• Alignment Length: 1134
• Identity: 511/1134 - (45%)
• Similarity: 704/1134 - (62%)
• Gaps: 99/1134 - (8%)

  Fly    43 KGNQAGDDDVKAVTKGVKKLNVKGKGKAGKNDDSDEEEVVPAKGKASKKSAFELLMDDDEQDEPA 107
            |.|:..|.|..|..|.:..|:|.....|  ..|.:|.:....|.||..|..|..|:|:.|.:|..
 Worm     8 KKNKDDDWDDDAAEKKLAALSVASNADA--QWDDEEPKKAKTKAKAPAKKGFAALLDESEPEENY 70

  Fly   108 AQESQSEKEEK---VVVSKPQKNEKKGKKAKRKGKD---DDDEDLDKVLAELQAEYAGEAAPAAT 166
            ::..:.|.|.|   .|..||:|.:|:.|||.:|||.   |::||||.:||..|.:...:.|..|.
 Worm    71 SEPPEPEPEVKKAAKVPEKPEKEKKEKKKAGKKGKKGEADEEEDLDALLASFQEKDGPKVAEVAP 135

  Fly   167 TVVSPEELADEFSKKKKNKKQAKQAAVAENAGEDEASDDEEGGSTVKSAAQKKKEKKERQKREAA 231
            ..|:..|.|....||.|.||:
 Worm   136 EAVAAPEAAPVDDKKSKKKKE-------------------------------------------- 156

  Fly   232 LAKQKAATEPKPKPVEKPAPEPEPVATEEVQPAAEEEEEKSSKNKKKGKKDKKAEPEEEKKDAKK 296
                        ||||||   .||...|:.....:|:::.....||:.|||||.|.|:::|:.||
 Worm   157 ------------KPVEKP---EEPAVEEDSDGEGDEKDQDKKDKKKQKKKDKKKEKEKKEKEDKK 206

  Fly   297 KGMSA-----SMVAAMQEQLRKRKEEEERLEREEAERIRLEDEREEARLEAVRLEAERKEKKKQR 356
            .|..|     |::..|.::.::.:||:|||::|:.||   :.:.|:.|||....|.||||..|:.
 Worm   207 GGAGAKKGNLSLIKEMLKKQQEEREEQERLQKEQQER---DAQEEKERLEREHAEKERKEAAKEA 268

  Fly   357 EAERKARLKAEGKLLTKKQKEDRARAQALLESLKAQGLEIPDPNDKRPTRPG------------T 409
            :.....|.||.|...|..||..:|.|   ||.|:|.|..:|.|.|.......            |
 Worm   269 KRLEVERQKAAGTYQTAAQKRQQALA---LEKLRAAGHSVPAPRDSTEDEAAGVKKSFVYVDEKT 330

  Fly   410 RIRPNKKKGPKEEAAEEEAKAAEAEAQSAAAAAAAAAAE----KAEEERVKE---SWDATDSEAE 467
            :.:..:||..:.|...:...||||.|.::|:|:|.|:||    ..|:|:..|   .|:...||.|
 Worm   331 KAKQEEKKRRRLEKLGQLPVAAEASADASASASAEASAETPSTTPEQEKTPEPVDDWEMAHSEDE 395

  Fly   468 PEPEEETSQATNNGKTEAAEEENEDTDDDEDDDDDEDDSDDSDGDSDDSREVSVLANDP-ESRRL 531
            ...::|..:......::|.|:::|::.|:|.|::.||..:..:..|||..:......:. |.|..
 Worm   396 ASAQKEKPKKPATKASDADEDDDEESSDEESDEESEDSEESEESSSDDDEDRGGQQKETVEERME 460

  Fly   532 RAEARILKRQAEAEKKRSTDELRAGVVCVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATN 596
            |.:.||.||:..|:..||||.||:.|:||||||||||||:||.:|||:||..||||||||||||.
 Worm   461 RIKLRIAKRKEVAQANRSTDNLRSPVICVLGHVDTGKTKMLDTIRRTNVQQGEAGGITQQIGATE 525

  Fly   597 VPIEAIKEQTKYVKAAAGFEHRLPGLLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQT 661
            ||.|||||:.:.|:.....:.::||.|||||||||||||||.|||||||.|||||||||||||||
 Worm   526 VPAEAIKERCRQVRGFLQDQMKIPGFLIIDTPGHESFSNLRTRGSSLCDFAILVVDIMHGLEPQT 590

  Fly   662 IESIQLLKKKKCPFIVALNKIDRLYDWKQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQG 726
            |||::||.|.|.||::|||||||||:::...|:||.::||.|:...|.||::|...::::||||.
 Worm   591 IESLKLLIKGKTPFVIALNKIDRLYEYESNPRKDVYELLKSQKPRVQAEFKERFNQIVVEFAEQE 655

  Fly   727 LNAALF-YENTDPKTYISLVPTSAISGEGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKAL 790
            ||..|. ::|.:...|:.:|||||..|:|:|||:..|.:..|...|::|.:||||.|||:||||:
 Worm   656 LNVTLSNHKNAEDSDYVCMVPTSAFLGDGIGNLMAFIINQTQTKYAQKLAFSEELDATVMEVKAI 720

  Fly   791 PGLGTTIDAILINGKLREGQTMVVAGTDGPIVTQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGV 855
            ||||||||.||:||.:|.|..:|:.|:||.|.||:|.||||:|:|||||||.|:.|||||.|:||
 Worm   721 PGLGTTIDVILVNGTMRAGDVIVLTGSDGAITTQVRELLMPKPLKELRVKNEYIHYKEVKGARGV 785

  Fly   856 KIAAKDLEKAIAGINLLIAHKPDEVEICTEEVARELKSALSHIKLAQTGVHVQASTLGSLEALLE 920
            |:.||:|||.:||:.:.|..:.|||:....|..|:|.:||..|:....||:||||||||||||||
 Worm   786 KVLAKNLEKVLAGLPIYITDREDEVDYLRHEADRQLANALHAIRKKPEGVYVQASTLGSLEALLE 850

  Fly   921 FLRTSKIPYSAIRIGPVVKRDVMKASTMLEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADI 985
            ||::..||||.:.||||.|:||.|||.|.||:|:||.:||||||:|||||..||..|||:|||||
 Worm   851 FLKSQNIPYSNVNIGPVHKKDVQKASAMKEHKAEYACVLAFDVKVEREAQIFADHEGVKVFQADI 915

  Fly   986 IYHLFDKFTAYREELKQKKREEFRSVAVFPCKLRILPQFVFNSRDPIVMGVMVENGIVKVGTPIC 1050
            ||||.|.|..||||||:|.|.:...:|:||||||:||..|:|:|:|||.||.:|.||||.|||||
 Worm   916 IYHLQDAFLKYREELKEKARRDNEHLAIFPCKLRVLPNHVYNTRNPIVCGVSIEAGIVKRGTPIC 980

  Fly  1051 VPSKEFVDIGIVTSIESNHKQIEFARKGQEICVKIDPIPGESPKMFGRHFEADDMLISKISRQSI 1115
            |||||.:.:|.::|::.|::::..|::|:|:|:||:...||:|:::||||..:|.|.|:::|:||
 Worm   981 VPSKEGIFLGTISSVQRNNEEVPSAKQGEEVCIKIENTTGEAPRLYGRHFTHEDPLYSRVTRESI 1045

  Fly  1116 DACKDYFRDDLIKADWALMVELKKLFEIL 1144
            |.||.|||:||.||||.|:|:||||.|||
 Worm  1046 DICKKYFREDLTKADWQLVVQLKKLLEIL 1074

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_001246599.1	PRK04004	553..1129	CDD:235195	339/576 (59%)
		IF2_eIF5B	557..769	CDD:206674	128/212 (60%)
		aeIF5B_II	779..889	CDD:293904	65/109 (60%)
		IF-2	890..993	CDD:288813	68/102 (67%)
		IF2_aeIF5B_IV	1016..1110	CDD:293911	48/93 (52%)
iffb-1	NP_497536.2	AP1AR	<237..>283	CDD:374071	17/48 (35%)
		InfB	480..1073	CDD:392272	350/592 (59%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160167063
Domainoid	1	1.000	257	1.000	Domainoid score	I1086
eggNOG	1	0.900	-	-		E1_COG0532
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	905	1.000	Inparanoid score	I190
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53766
OrthoDB	1	1.010	-	-		D1124591at2759
OrthoFinder	1	1.000	-	-		FOG0002309
OrthoInspector	1	1.000	-	-		oto19656
orthoMCL	1	0.900	-	-		OOG6_101241
Panther	1	1.100	-	-	LDO	PTHR43381
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R593
SonicParanoid	1	1.000	-	-		X1735
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	15	14.800