Protein Alignment eIF5B and eif5b

DIOPT Version :9

• Sequence 1: NP_001246599.1
• Gene: eIF5B / 44261
• FlyBaseID: FBgn0026259
• Length: 1144
• Species: Drosophila melanogaster
• Sequence 2: XP_031752875.1
• Gene: eif5b / 100492012
• XenbaseID: XB-GENE-6258156
• Length: 1209
• Species: Xenopus tropicalis
• Alignment Length: 1236
• Identity: 646/1236 - (52%)
• Similarity: 825/1236 - (66%)
• Gaps: 126/1236 - (10%)

  Fly     7 GKKEILTKVEGGSSVDEISDVDSDQLSLNNKQNHALKGNQA---------GDDDVKAVTKGVKKL 62
            |||:   |.:...|..:  |||.|.|:...:...|.|..:|         .|.:.:.:.|.:::|
 Frog     2 GKKQ---KTKSDESAKD--DVDVDALAAEIEGAGAAKDQKAKGKKKKGKKQDFNEEDILKELEEL 61

  Fly    63 NVKGKGKAG------KNDDSDEEEVVPAKGKASKK--SAFELLMDDDEQDEPAAQESQSEKEEKV 119
            :::.:|..|      |..::|..|.||.|....||  .|.:..:|:||.:.....|:::.|.:|.
 Frog    62 SLEAQGGKGDKELVSKKAETDNIEPVPVKQDKKKKGQKAKKTNLDEDEDNSENEMETKTAKPKKA 126

  Fly   120 VVSKP-------QKNEKKGKKAKR-KGKDDDDEDLDKVLAEL---QAEYAGEAAPAATTVVSPEE 173
            ..|||       ..|:...:.:|: |||:.......:|:.|.   :.|::|::.          |
 Frog   127 DKSKPIDMSESDDDNDNFNQTSKKAKGKNQSASKKLQVVEEANDQKEEHSGDSG----------E 181

  Fly   174 LADEFSKKKKNKK--QAKQAAVAENAGEDEASDDEEGGSTVKSAAQKKKEKKERQ--KRE---AA 231
            .:||||:.||.||  :||.|...|:|       ||:|...:|:|||||.|||||:  |||   |.
 Frog   182 ESDEFSQGKKGKKNQKAKSAPKVESA-------DEDGEFKIKTAAQKKAEKKERERKKREEEKAK 239

  Fly   232 LAKQKAATEP---KPKPVEKPAPEPEPVATEEVQ----PAAEEEEEKSSKNKKKGKKDKKAEPEE 289
            :.|||...||   |....|..||:.|..|..|::    .|||.|.:..::..||.|..||.:.|:
 Frog   240 VKKQKEKDEPEGGKKDAAETAAPKKEAKAANELEITTAAAAEGEADDDNEGDKKKKDKKKKKVEK 304

  Fly   290 EKKDAKKKGMSASMVAAMQEQLRKRKEEEERLEREEAERIRLEDEREEARLEAVRLEAERKEKKK 354
            .:||.|||..|.::|.||||.|.|.|||||||:|||.||:|..:|.|..|||..|||.|||||:|
 Frog   305 TEKDDKKKRPSTAVVKAMQEALAKMKEEEERLKREEEERLRRLEEMEAKRLEEERLEQERKEKRK 369

  Fly   355 QREAERKARLKAEGKLLTKKQKEDRARAQALLESLKAQGLEIPDPN----------DKRPTRP-- 407
            |:|.|||.|||.|||||||.|:|.||||:|.|:.|:|||:|:|..:          ||:..:|
 Frog   370 QKEKERKERLKKEGKLLTKTQREARARAEATLKVLQAQGVEVPSKDSVPKKKPIYEDKKKKKPQQ 434

  Fly   408 --GTRIRPNKKKGPKEEAAEEEAKAAEAEAQSAAAAAAAAAAEK----AEEERVKESWDATDSEA 466
              ..:..|.:...|.|...|:.|.|.|..|....:...:..:|.    .||:...:.|:|..|:.
 Frog   435 QTPVKQEPMEVSSPVEAVIEQPAPAKEETAVPVESPGNSVDSESDKEATEEDAGVDDWEAIVSDE 499

  Fly   467 EPEPEEETSQATNNGKTEAAEEENEDTDDD---EDDDDDEDDSD--------------------- 507
            |.|.|.:|.......|.|..|||:||.:|:   |::|:||.||:
 Frog   500 EREKETKTIHIEVQEKNEEEEEEDEDEEDEGESEEEDEDEGDSEGTEDGKSSDEKPQESQTVAPK 564

  Fly   508 --------------DSDGDSDDSREVSVLANDPESRRLRAEARILKRQAEAEKKRSTDELRAGVV 558
                          :|:.||||.|....:|.|      :|:.||.||:.|.:|..:...|||.|:
 Frog   565 LQEKKPSRELSSESESESDSDDDRTKEEIAYD------KAKRRIEKRRLENQKTVNIQVLRAPVI 623

  Fly   559 CVLGHVDTGKTKILDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTKYVKAAAGFEHRLPGLL 623
            ||||||||||||||||||.|||||||||||||||||||||::|||||.|.||.......::||:|
 Frog   624 CVLGHVDTGKTKILDKLRHTHVQDSEAGGITQQIGATNVPLDAIKEQAKMVKNFDRENIKIPGML 688

  Fly   624 IIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESIQLLKKKKCPFIVALNKIDRLYDW 688
            |||||||||||||||||||||||||||||||||||||||||:.|||.||||||||||||||||||
 Frog   689 IIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESLNLLKNKKCPFIVALNKIDRLYDW 753

  Fly   689 KQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQGLNAALFYENTDPKTYISLVPTSAISGE 753
            |:....||...||:|:.||:.||::|.|.:|::||:|||||||||||.||:|::|||||||.:|:
 Frog   754 KKGPDADVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGD 818

  Fly   754 GMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGLGTTIDAILINGKLREGQTMVVAGTD 818
            |||||:.::.|..|.||.|||.:||||:|.|:|||||||:|||||.|||||:|:|...::|.|.:
 Frog   819 GMGNLISLLVDLTQTMLNKRLAHSEELRAQVMEVKALPGMGTTIDVILINGRLKESDIVIVPGVE 883

  Fly   819 GPIVTQIRSLLMPQPMKELRVKNAYVEYKEVKAAQGVKIAAKDLEKAIAGINLLIAHKPDEVEIC 883
            ||||||||.||:|.|||||||||.|.::|||.|||||||..||||||:||:.||:||..||:.:.
 Frog   884 GPIVTQIRGLLLPPPMKELRVKNQYEKHKEVIAAQGVKILGKDLEKALAGLPLLVAHSEDEIPVL 948

  Fly   884 TEEVARELKSALSHIKLAQTGVHVQASTLGSLEALLEFLRTSKIPYSAIRIGPVVKRDVMKASTM 948
            .:|:..|||..|:.|||.:.||:||||||||||||||||:||::||:.|.||||.|:||||||.|
 Frog   949 RDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVM 1013

  Fly   949 LEHEAQYATILAFDVKIEREAQEMADSLGVKIFQADIIYHLFDKFTAYREELKQKKREEFRSVAV 1013
            |||:.|||.||||||:||||||||||||||:||.|:|||||||.||.||::.|::|:|||:.:||
 Frog  1014 LEHDPQYAVILAFDVRIEREAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAV 1078

  Fly  1014 FPCKLRILPQFVFNSRDPIVMGVMVENGIVKVGTPICVPSKEFVDIGIVTSIESNHKQIEFARKG 1078
            ||||:||||||:|||||||||||:||.|.||.|||||||||.|||||||||||.|||.::.|:||
 Frog  1079 FPCKIRILPQFIFNSRDPIVMGVVVEAGQVKQGTPICVPSKNFVDIGIVTSIEINHKSVDMAKKG 1143

  Fly  1079 QEICVKIDPIPGESPKMFGRHFEADDMLISKISRQSIDACKDYFRDDLIKADWALMVELKKLFEI 1143
            ||:|:||:||||||||||||||||.|.|||||:||||||.|::|||::.|:||.|:|||||:|||
 Frog  1144 QEVCIKIEPIPGESPKMFGRHFEATDFLISKITRQSIDALKNWFRDEMQKSDWQLIVELKKVFEI 1208

  Fly  1144 L 1144
            :
 Frog  1209 I 1209

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eIF5B	NP_001246599.1	PRK04004	553..1129	CDD:235195	416/575 (72%)
		IF2_eIF5B	557..769	CDD:206674	157/211 (74%)
		aeIF5B_II	779..889	CDD:293904	71/109 (65%)
		IF-2	890..993	CDD:288813	78/102 (76%)
		IF2_aeIF5B_IV	1016..1110	CDD:293911	75/93 (81%)
eif5b	XP_031752875.1	InfB	618..1191	CDD:392272	416/572 (73%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	318	1.000	Domainoid score	I1259
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1116	1.000	Inparanoid score	I230
OMA	1	1.010	-	-		QHG53766
OrthoDB	1	1.010	-	-		D1124591at2759
OrthoFinder	1	1.000	-	-		FOG0002309
OrthoInspector	1	1.000	-	-		oto105036
Panther	1	1.100	-	-	LDO	PTHR43381
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R593
SonicParanoid	1	1.000	-	-		X1735
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	12	12.110