Protein Alignment nbs and NBN

DIOPT Version :10

• Sequence 1: NP_651973.4
• Gene: nbs / 44259
• FlyBaseID: FBgn0261530
• Length: 818
• Species: Drosophila melanogaster
• Sequence 2: NP_002476.2
• Gene: NBN / 4683
• HGNCID: 7652
• Length: 754
• Species: Homo sapiens
• Alignment Length: 881
• Identity: 208/881 - (23%)
• Similarity: 346/881 - (39%)
• Gaps: 229/881 - (25%)

  Fly     9 EKFVLFPGKKVYTIGRLATDLIVAQDLSISRNHAQLLIQ------TEADGDDTLHIEDLGSRYGT 67
            |.:.|..|.: |.:||....:::..|.|||||||.|...      ::.|....|.::| .|:|||
Human    14 EPYRLLTGVE-YVVGRKNCAILIENDQSISRNHAVLTANFSVTNLSQTDEIPVLTLKD-NSKYGT 76

  Fly    68 FIFPKNSQKPRKVPAKTSTPLPVGTRLRFGANMSIWQVTQLKLVTTVSALTRSEVQELTKMLEPM 132
            |:   |.:|.:...::|   |..|..:.||...|.:::....||...|.|..|....|.:.:..:
Human    77 FV---NEEKMQNGFSRT---LKSGDGITFGVFGSKFRIEYEPLVACSSCLDVSGKTALNQAILQL 135

  Fly   133 GGTVTSNWTEECSHLTMNEVSVTVKLLHAMLENKPIVTFPYWRKMLQAAQSIHVKEGWPQPEDYQ 197
            ||...:||||||:||.|..|.||:|.:.|::..:|||...|:.:.|:|.:|   |:..||.|.:.
Human   136 GGFTVNNWTEECTHLVMVSVKVTIKTICALICGRPIVKPEYFTEFLKAVES---KKQPPQIESFY 197

  Fly   198 P---------TNIDVTWRPERTRLFAGKTFVFMNRKHFDMYGSVVQKAGATCKDI---NSGVRKT 250
            |         .|:|::.|.||.::|.||||:|:|.|......|.|...|...:.|   |......
Human   198 PPLDEPSIGSKNVDLSGRQERKQIFKGKTFIFLNAKQHKKLSSAVVFGGGEARLITEENEEEHNF 262

  Fly   251 FLTKSDVIVIQYVPSSQSQATES----INSIQDRYILEQNGRRIIQEYEIGMALIHCSITEFCNP 311
            ||.....:|...:.:||:...:.    |.||.|  :|::.|.|.|.|.|||:|:|..:...:|:|
Human   263 FLAPGTCVVDTGITNSQTLIPDCQKKWIQSIMD--MLQRQGLRPIPEAEIGLAVIFMTTKNYCDP 325

  Fly   312 T-HKFISDSLPTT-------------ESVTSSMAFNSSIIVPNTERHSAQSNATPISELVVPESI 362
            . |.  |..|.||             |.:..|...|::..|.:||  |.|::...:||.  |:.|
Human   326 QGHP--STGLKTTTPGPSLSQGVSVDEKLMPSAPVNTTTYVADTE--SEQADTWDLSER--PKEI 384

  Fly   363 ECEMEQDASKPHSEDQASLRKRSHASTVDSSDEEKKSTLSKRAKSDIATKLTMKSKNAILLDSSL 427
            :....:...:..|:|..::::  ...|..:::....:||:|           |:..|
Human   385 KVSKMEQKFRMLSQDAPTVKE--SCKTSSNNNSMVSNTLAK-----------MRIPN-------- 428

  Fly   428 EEDVTPAPAPAPVQRVTRQSKAIAEEKSVHPPVPAASKHITRKTKQVFCVDSSDEENENARKPK- 491
             ..::|...|:     ..:||..|.::.....:....:..|:|.::       ||||:.....|
Human   429 -YQLSPTKLPS-----INKSKDRASQQQQTNSIRNYFQPSTKKRER-------DEENQEMSSCKS 480

  Fly   492 ----------ETPAPTIPSMAKKKTE-----APVATRISPRL-NGKSLATNITNQPADKHAVP-- 538
                      |...|..||:.|.|.:     .||.|.....| ....|.:.:.|..:..||..
Human   481 ARIETSCSLLEQTQPATPSLWKNKEQHLSENEPVDTNSDNNLFTDTDLKSIVKNSASKSHAAEKL 545

  Fly   539 ---AKRPVLSVASSDEEDEGDLFQFRKSPQKPAETVVQPRIAGKGNAPARISVVDFLEKSQAQEP 600
               .||.:..||..||..| .||:..| |:...:..||
Human   546 RSNKKREMDDVAIEDEVLE-QLFKDTK-PELEIDVKVQ--------------------------- 581

  Fly   601 APVPPQLESQSQTQPRKRLRLEL-LNESDSDDC----DNLFNFADSKKKRK--------TQEAQR 652
                   :.:.....|||.|::: .|::.||:.    ..:....:..|||:        .:|...
Human   582 -------KQEEDVNVRKRPRMDIETNDTFSDEAVPESSKISQENEIGKKRELKEDSLWSAKEISN 639

  Fly   653 NDDSTDG-----------------LFNFNSERPSDHDDEDSRLTEPFVPETESKKQSKYIVAPRR 700
            ||...|.                 :.|..|..||..:|:..:|        ::.|:.|.:..|..
Human   640 NDKLQDDSEMLPKKLLLTEFRSLVIKNSTSRNPSGINDDYGQL--------KNFKKFKKVTYPGA 696

  Fly   701 DRPKKVDISGWLSCSRLNDNIKSEIDADSVKMETSIKADPDEEQWLAAMKDSIEVRMCNLNIVIR 765
            .:...: |.|            |::.|...:..|.:      |:||   :..:||         :
Human   697 GKLPHI-IGG------------SDLIAHHARKNTEL------EEWL---RQEMEV---------Q 730

  Fly   766 SQEEVDASLEDSVNKHGGRKNFKKFVKTKNPHPQKR 801
            :|...:.||.|.:.::             ||:.::|
Human   731 NQHAKEESLADDLFRY-------------NPYLKRR 753

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nbs	NP_651973.4	FHA_NBN	1..107	CDD:438719	31/103 (30%)
		BRCT_nibrin	109..180	CDD:349372	27/70 (39%)
		NIBRIN_BRCT_II	207..311	CDD:465151	35/110 (32%)
		PHA03247	<434..616	CDD:223021	38/203 (19%)
NBN	NP_002476.2	FHA_NBN	2..110	CDD:438719	31/103 (30%)
		Mediates interaction with SP100. /evidence=ECO:0000250|UniProtKB:Q9R207	111..328		73/221 (33%)
		BRCT_nibrin	111..184	CDD:349372	27/72 (38%)
		NIBRIN_BRCT_II	217..325	CDD:465151	35/109 (32%)
		Interaction with MTOR, MAPKAP1 and RICTOR. /evidence=ECO:0000269|PubMed:23762398	221..402		53/188 (28%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	326..346		5/21 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	396..415		3/20 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	430..478		11/59 (19%)
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:16188882	461..467		2/5 (40%)
		Nbs1_C	683..744	CDD:462531	17/91 (19%)
		FxF/Y motif. /evidence=ECO:0000269|PubMed:15758953, ECO:0000269|PubMed:35076389	740..749		2/21 (10%)