Protein Alignment spen and din-1

DIOPT Version :10

• Sequence 1: NP_722615.1
• Gene: spen / 44205
• FlyBaseID: FBgn0016977
• Length: 5560
• Species: Drosophila melanogaster
• Sequence 2: NP_496485.2
• Gene: din-1 / 174782
• WormBaseID: WBGene00008549
• Length: 2407
• Species: Caenorhabditis elegans
• Alignment Length: 2986
• Identity: 566/2986 - (18%)
• Similarity: 970/2986 - (32%)
• Gaps: 1135/2986 - (38%)

  Fly   453 SSYSSSHSSSSSSHSSSHSHASSPVQSSGNCAMAEGRSSRTVNSVTVTSNSSNPSGTAVTVSSAG 517
            ||.:|..|:.||..|||..:|....|..|:...||.:|....|.:                 .|.
 Worm    92 SSMASMSSTPSSGQSSSPRNAELSPQRYGDTRGAEVKSPSFRNQM-----------------EAR 139

  Fly   518 VGGGCGSSSSSSSSS---------SSSGSSCLTANPVVHSEDNRPLAIRVRNLPARSSDTSLKDG 573
            .||...|..|....|         .|..::|     ||:.              .:|..|..:|
 Worm   140 RGGPHLSVQSQQRHSREYWPIPEFPSESTAC-----VVYE--------------IQSGSTPERD- 184

  Fly   574 LFHEYKKHGKVTWVKVVGQNSERYALVCFKKPDDV--EKALEVSHDK---HFF-----GCKIEVE 628
            ||...|||.|.:.|                 |.|:  |...|....|   |::     |.|.:..
 Worm   185 LFELVKKHSKRSGV-----------------PIDIQLESTTEPGWKKARVHYYRLDTDGLKADKS 232

  Fly   629 PYQGYDVEDNEFRPY-----EAELDEYHP---------------KSTRTLFIGNLEKDITAGELR 673
            ...|   ...:||.|     |.:..:.||               |::.::.:.:|::. :....|
 Worm   233 LILG---RPPKFRVYYPTSGEQKHPQCHPSTSYAIPKLKGDHLLKASCSVHVPHLDRH-SPDHYR 293

  Fly   674 SHFEAFGEIIEIDIKKQGLN-AYAFCQYSDIVSVVKAM------RKMDGEHLGSNRIKLGFGKSM 731
            ..||::|::|::|:.|...| |:|..|:::|....||:      :.|..:...|:||.:.:   :
 Worm   294 RRFESYGQVIDVDMVKSNDNKAFAVVQFTNIDDAQKALQDTNIPKPMSYQSRPSHRIIIFY---L 355

  Fly   732 PTNCVWIDGVDEKVSESFLQSQFTRFGAVTKVSIDRNRQLALVLYDQVQNAQAAVKDMRGTILRR 796
            |..|     .:|::. ..::|...|   :..:.:|...:.|::..|.::.|...:|.|:  ::.|
 Worm   356 PIEC-----TNEEIM-LIIRSLSDR---IVDICVDWWDRSAVITLDDMEPANLLLKRMK--LVGR 409

  Fly   797 K-----KLQVDFASRECQDAF-----YDKQEKQQQQSSGSNPRFSRYESSASSLQSRSRASSFSR 851
            .     |:.|||    |.|.|     ..|:|..:..:..|:|      :|.|.....|.:.|.||
 Worm   410 NNFGEHKVAVDF----CSDRFNLYFINRKKENIEVAARSSSP------TSKSENDQGSSSPSSSR 464

  Fly   852 HQNNSNDDCSPINTPGGASSGISSASNLINQSTSINISNIGTNACSAMPAPSLASAVVSCNVNAS 916
            .:.|.:|   |:.|.       ||..:..||....|                          |||
 Worm   465 DRQNLHD---PLQTR-------SSVEHHTNQEDQEN--------------------------NAS 493

  Fly   917 GTVPASTSMPSGVSSSSSSLPMSPAALAQRHRMVRNARQTVDCDFNEVGRLRFRSSEEVSGGAGN 981
            |             |.|||                      |.|           |||   |:.:
 Worm   494 G-------------SDSSS----------------------DSD-----------SEE---GSSS 509

  Fly   982 STQFEDVRCDSPVTARQGSAVNCFTGPTAAVGESIDGTLNNNQITGGAEGFTGSGGSILSRRRCG 1046
            |.:..|.:.|                    |.|..|..:.:.:                 :|...
 Worm   510 SNEDSDEQND--------------------VDEEDDEDVVSEE-----------------KRHEP 537

  Fly  1047 KTPKDLHPVHNQRIQLAEQVEECPSSGDEGVVSPRKRIKMDYHHHHHHSNASGVESTGEHSSINK 1111
            :..|...|.:..|.:         |:||:......:|........||.::.| .|...|......
 Worm   538 EEGKSSSPGNGHRDE---------SNGDKDHEDSSERFSQPSTSSHHETSHS-PEKDSEAYQSRS 592

  Fly  1112 PSPLLLSNCDVIHDPLNRKSEIRRVSETPSGSPS--IKFPGHLPSAPQSLMLSCR--RPSIDVGA 1172
            .|||...:....::.|..|...:..|.|.|.:.|  ::....:|..|.:.||...  ||.|||
 Worm   593 FSPLNYQSQSPGYEFLESKEIKQEFSPTTSSASSSDLELDMEMPDNPLTRMLERMHWRPFIDV-- 655

  Fly  1173 LSALSSSSAFRHGIVGASSMDQQHMMNASAAAKRRRVTTTMQQPSSSSTTNSSSGSGLGGISSLT 1237
                   |:|      .:.:|:...:|..|.|...:.|
 Worm   656 -------SSF------VNRIDEIVELNQKARASYEKFT--------------------------- 680

  Fly  1238 PADEYHHHVSRGRGHQLHSHHSHEASGGESADGSRPGTPLCDERPEVLPTEPRRLPPPRE----- 1297
                                             .|| .|.|: ..|||..:......||:
 Worm   681 ---------------------------------GRP-FPKCN-NDEVLSIQKIVFHEPRDYYYYE 710

  Fly  1298 ----RVRERTRDVMWLPLPKFGVL-FFQQQQSRSSGGGGAGNSYLQQQLGGGSTGGLGCIGAASS 1357
                .:..|.||  |..|.....| .|:...|:..|         :.|..||.|.|
 Worm   711 NPCSELEVRIRD--WRKLSDTADLDDFRATDSKELG---------RDQPAGGRTSG--------- 755

  Fly  1358 SACSLNNSSLNASQGMGSCSGSTFLPSPSSRYWRSSSHHQNQQNNHQQQSQQLHGSSSSNTCLMA 1422
                  ..||:.|:              ::|....|:||.      .:.:|:.|.       |..
 Worm   756 ------RPSLDESR--------------TNRLSFDSTHHP------AELAQRSHS-------LCI 787

  Fly  1423 SPARPRSLSSNS-----SDSDVPGQNAGGSPSLDERLRNFEENYERWSGGSSREHISGHTPSSAT 1482
            .|..|.:....|     :.:.:||.:   .||.              |||.:       ||.|:.
 Worm   788 GPMTPSTPFPTSQPLLVNTTHLPGTS---QPST--------------SGGIT-------TPRSSQ 828

  Fly  1483 PSWQLSMHMNLSTGLNSHQTSSASGNSNSSSGTVSSSASNSRHKFLDIDELQPSDIVKSVLAKKS 1547
            |.       .|.:.::.|       ||.||:|                   :|:.|  ..|..:|
 Worm   829 PP-------PLMSPVSRH-------NSMSSTG-------------------RPASI--QTLRHQS 858

  Fly  1548 VFDDDFQRLNKNQWYDPSSSDFALGSSSNIVTGSSLVANVSRHPGGPCSGNTSPALP----NLAA 1608
            |.            :.|                     :||..|         |.:|    .:.|
 Worm   859 VM------------FPP---------------------DVSIPP---------PPIPPTHDEMMA 881

  Fly  1609 TKATPIIGNCSGGLGNSTGSKSAGLLQRLSSLSPMNSPQASMSPYNSPSPSPSVGGVTACLGQLT 1673
            .:.||.....|                  .::.|:.||     |:.:|..:           .||
 Worm   882 PRGTPPSRRSS------------------ETMVPLRSP-----PFGTPIQN-----------LLT 912

  Fly  1674 KPAAPGT-ASAGLSGGTAASSSSPAANSGPTKGLQ-YPFPSHPPLPNTAAPPPAVQPAPPPLPEM 1736
            .|..|.. ..|..|.||.:.|||  |:|.|...:. .|....|....|:.||.
 Worm   913 MPIVPPPHLIAATSTGTHSVSSS--AHSTPRHSISGTPVHCEPSNSKTSQPPT------------ 963

  Fly  1737 GKQSRLTGQSSGNNLTKSLSVPDGPQSSPARVQLQKSASVPGSTNVGAPSSLSLDSTTASVETSA 1801
                                    |:|.|.:||::.             .::|....:.::....
 Worm   964 ------------------------PKSRPEKVQIRH-------------DTISKSGPSNAINALQ 991

  Fly  1802 SISSSTSNGNSSLTSAAIHVQKPQQSTFVEEEHTKKSGTSTSQSSSSSSKKISSTHDKLHSKHNN 1866
            :.|.|.::|:                       .|||..||.....:.|..::..      ..|.
 Worm   992 ARSQSMTSGD-----------------------PKKSAPSTPVVRDAGSDLVAQI------MSNQ 1027

  Fly  1867 RSESDKKIKKSDKNASSSDKRKNSSTSQSSKSATPRIEDDSSEADDTADKAEKNQRHEKEKKERQ 1931
            .:...:|:.:.:|.:|:....:|.....|:.::||.....|:              |:...|:::
 Worm  1028 PNLGLRKLPRIEKKSSALQNIQNHQPPHSNANSTPSTPSTST--------------HQAMFKDKE 1078

  Fly  1932 EKREKDLRKQVEREEKDRKAQQEEREKEDRKAKEEEKEREREKKAQEDREKKEREERELREKEQR 1996
            ::|:|   |:.|:||::|:|::|.:.||.::.:.:.||.||.|:.:::|::::||:::.|::.::
 Worm  1079 KERKK---KEKEKEEREREARREMKRKETKEERNKRKEMERAKRLEDERQERKREKKKERDERKK 1140

  Fly  1997 DKEQKEKEIREKDLREKEQRERDNREK-------ELRDKDLREKEMREKEQREKE---------- 2044
            :||:..|:..::.|::|:.|:.|:.::       || |.|:| |..:|..|.||:
 Worm  1141 EKEKVRKKAEKEKLKKKKHRKGDSSDESDSDSNDEL-DLDVR-KSTKEMTQEEKDHQLALLLSKG 1203

  Fly  2045 -------LHREKDQREREH----REKEQSRRAMDVEQEGRGGRMRELSSYQKSKMDIAGEASSLT 2098
                   ..|..|:|..:.    ::|.|.||.:....:..||:..:           .|.:|
 Worm  1204 GIIENLKSRRRSDKRAHDSFEKMQQKSQQRRVLIESSDDEGGKDGD-----------KGNSS--- 1254

  Fly  2099 AIDCQHNKENAMDTIAQGTPGASPSTPSDNTP------KERSR-KLS-------RNSPVRLHKRR 2149
                  |.|.:....|...|..:|.:.|::..      |||.: :|:       .|....:|::|
 Worm  1255 ------NGEESDSEKADLPPPPAPPSLSESADQRLKVLKEREKGELTTSSDDEDHNDAGEIHQQR 1313

  Fly  2150 LSSQESNHSAGGGGSCGGSSHQIHHEDYVKRIRMENSQNISV-HSSNQRLNDRRDSK---EHKSS 2210
            |:....|.......:...|..|...::..||:|.::|::.:. |......:|::..|   ||:.|
 Worm  1314 LTEDRENRKRQKSLTAYSSDEQGERKNVPKRMRRDDSEDAAAKHPGWSAKDDQKQRKRKLEHRRS 1378

  Fly  2211 SFKEDKNSSSHISR--PHGCGGSSASSSKHHHRRDKHHQKGSASSIETNSSIEVVVDPISQTKHN 2273
            |..|.|.::....|  ||    ...|.       ::..:.||.|..::.||          |..|
 Worm  1379 SEDESKKNAKRDFRDIPH----EDVSD-------EEETEDGSRSRRQSTSS----------TISN 1422

  Fly  2274 LNTSEEELQSHQ----------------PKREKEREHFSSHANSSSSRHKSKRDHHHHREKKRHS 2322
            :...|.:.:|.:                ||....: |.|...::||::..|..||
 Worm  1423 VTAKERKEKSGKTPLRIVPEPTGTPLLSPKILSPK-HLSPKTSTSSTKRSSISDH---------- 1476

  Fly  2323 VAESTNTDEEHTPQQHNPHRRISAAGSGSAGELSSAATNTSSGKLHHQHHRRSVERKSSRGSDEG 2387
                   :...:|:|.|             ...||.:|.|:|.|    |...|:..|
 Worm  1477 -------ENLISPRQRN-------------RTTSSTSTATTSSK----HEALSIPEK-------- 1509

  Fly  2388 HHSSSKSLRAKLMMLSSADSDDTDDASKKHSIFDIPDDCPNVSMYDKVKARSCKNMQRQAEEKKI 2452
              ..|..:.|| ..:||.|.....|....:|..|.|.......|.                  ..
 Worm  1510 --PLSPPVTAK-SSVSSIDDPSIRDEFSMNSAADSPMSTTGRPMV------------------LT 1553

  Fly  2453 KAKFSQLKQSRAKKKRSTSYDGDSDTEFEDRQHRNSGSSSFHGRYPGLSSSDDDDDEETHQRR-- 2515
            ||.......:..|||.|:|...||          :|||||......|.:||||..|:|..::.
 Worm  1554 KAAMKAFNSTPPKKKNSSSGQHDS----------SSGSSSDSSSSDGSTSSDDSSDDEVPKQTEP 1608

  Fly  2516 ------ISSDSDAEHGGQDNQGASTLADANRVRQMQQNLRRLCDGDDSSEDEIRRNVMKHSHFGK 2574
                  ::||    :|..:|....|   .:.|.|..:........:.|||
 Worm  1609 VTSIPVVASD----NGSPENVVVET---PSIVSQTPREPEPFTISEQSSE--------------- 1651

  Fly  2575 RNSNSTRIASDSESQSQP-APDLTIKQEHPIAPAQEIKREQLSDEEQKFKSRHDSNSSIEERKLK 2638
                       ||.::.| .|:.::  |..:..:|.:  |.:|:|.:......||..::|.::..
 Worm  1652 -----------SEPEAVPECPEASV--EPQMETSQNV--EPVSEEHEDSHEHGDSEVAVESQQQP 1701

  Fly  2639 TE-REIKTEL-----------------GDFYNSSEYTYTGKLKEYSPETRKKHKKSKRRLKSSST 2685
            .| :|.|.||                 || .:|.|.:......|..|.|:.: :||...|.|...
 Worm  1702 LEHQEEKEELENKILDVAAEHHEEQVQGD-EDSVESSIPAPSDEPDPVTQAQ-EKSAHTLISDQE 1764

  Fly  2686 ADTSAAQTPLVMTPLTPSIFDVHSSSECKTKFDNFDDLKTECSSIPLEISAGERRKHKERKEKKR 2750
            .| .|.|          ||||...:.|    |..:.|...
 Worm  1765 TD-QAVQ----------SIFDEEEADE----FPQYPDFGI------------------------- 1789

  Fly  2751 EKLRNMTEATVPNSPTTNDTSSEKLSKEERHRLKKSKKSKSMDNSCNTKIYNSSGAHPSTSPSLP 2815
                              .|:.:::|.::.|.:   |.::.::|.....::         |||
 Worm  1790 ------------------STNEKEVSGKDPHNI---KPTEPLNNGHTDLLF---------SPS-- 1822

  Fly  2816 ATPTSAPSTAQTSKRGEDKMEFIFGIISDEEESQFPEQAETNKDIIPSSVSTTGPIVSAALQTYK 2880
               :||.::.:.|.:.||.||       ::.|....|:....:.:|...|..
 Worm  1823 ---SSAHASEKQSTKSEDDME-------EDSELVVMEKEVPMEQVIAQEVHV------------- 1864

  Fly  2881 QEPSTPNSKNEEAHIQLTVHEPEQQQQLERS--------RLSGGSSSSSHADRERHRREKREKKR 2937
              ||.|:...||..::.:....|:..::|.|        .:|...|..:......|..|..:..:
 Worm  1865 --PSEPSPMEEEVKLETSPVPKEEPIKMEESPEQTPTPDLISNNESQDTPGAVNNHLHENHDAVQ 1927

  Fly  2938 RE-KSQREQQNQIHQKSSKVETKVDDDNSVDMDEAGRALEAQLMSDFDTKPISEEATPSTAATYR 3001
            .. :.|...|:|:.|.|.:...      :.|..:.|..|            :|:::.||..::.:
 Worm  1928 TPIQLQPASQHQVAQPSPRPAV------APDSQQNGPVL------------VSQQSQPSPMSSQQ 1974

  Fly  3002 SDMTDVFRFSDNEDN----------------------------------NSVDMT---------- 3022
            |||......|..:.|                                  |..:||
 Worm  1975 SDMAQNLILSSKDINDLAAKLHKNPEALAQATRGDCSGIFQHLLLHAQGNGQNMTPEMLQLKAAF 2039

  Fly  3023 ---------KQGVKSEQQEQHKSKDKKKKKKRSK-----------EEKQEKLLQQQRRESLPNVA 3067
                     .|.::::.::|..:||:.|:::|.|           |:::||..:::.|:.|  .|
 Worm  2040 FAQQQENEANQMMQAKMKQQTINKDRIKEQERVKRMYEENERKVEEDRREKQRKEEERQRL--AA 2102

  Fly  3068 STSSAPPTPGKLTVNVQAASKHADLQLDA-----KHISSPPVCKPS--------PSLPCLIGDDD 3119
            :|::|       |:..|.|::....:.:.     :|:.|  :..|.        |.|...|..|.
 Worm  2103 ATAAA-------TMATQKAAEALKQKQEVPRHGFQHVLS--MMTPEARSLYEQFPGLSSYINRDS 2158

  Fly  3120 ----DDALHTPKAKPTTPSSRGNDGLTPSREKPRLISPIPKTP----TIANSSTLSTQSAE 3172
                :..||.|......|||..:....|           ||.|    ...|.:|:.....|
 Worm  2159 IGATNGVLHLPTQSIQRPSSTASTSSNP-----------PKAPLQPSASVNQNTIDPAEIE 2208

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
spen	NP_722615.1	RRM2_SHARP	555..628	CDD:409785	18/82 (22%)
		RRM3_SHARP	654..726	CDD:409786	19/78 (24%)
		RRM4_SHARP	727..803	CDD:409787	14/80 (18%)
		PTZ00121	<1912..2109	CDD:173412	52/224 (23%)
		DDRGK	1998..>2072	CDD:370664	24/101 (24%)
		Atrophin-1	3577..>3804	CDD:460830
		petO	4587..>4709	CDD:438570
		Atrophin-1	5107..>5374	CDD:460830
		SPOC_SHARP	5399..5560	CDD:439206
din-1	NP_496485.2	RRM_SF	15..75	CDD:473069
		RRM_SF	288..>335	CDD:473069	14/46 (30%)
		Herpes_BLLF1	<749..1008	CDD:282904	86/509 (17%)
		PRK12323	<1621..1970	CDD:481241	87/498 (17%)
		ARGLU	<2045..2100	CDD:405931	11/54 (20%)
		SPOC_SHARP	2219..2384	CDD:439206