DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment hbs and nphs1

DIOPT Version :9

Sequence 1:	NP_001286439.1	Gene:	hbs / 44129	FlyBaseID:	FBgn0029082	Length:	1235	Species:	Drosophila melanogaster
Sequence 2:	NP_001035777.1	Gene:	nphs1 / 692352	ZFINID:	ZDB-GENE-051102-1	Length:	1242	Species:	Danio rerio

Alignment Length:	1272	Identity:	360/1272 - (28%)
Similarity:	569/1272 - (44%)	Gaps:	138/1272 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MQFWITLTFAVHLATTICHVMAAVTAPTPAAQVAAPPVQKFHLTPHDLQILEGTDTLLRCEVSNR 65
            |..|::|.|     ::.|.:: .|.......|      |.|...|.::.:..|...||:|||...
Zfish     1 MDSWLSLVF-----SSGCFLL-LVDFKGVCGQ------QAFKTEPRNVTVRAGATALLKCEVLRA 53

  Fly    66 AGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIR 130
            :|.|||.|||..|.....:||:||||:..|.|:..|:|||.:.|||||..|:||||.:..:..|.
Zfish    54 SGAVQWVKDGLLLRPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIV 118

  Fly   131 ANAKLSIVAAPSSIVIEGYARNARVEVE----ERQNLTLHCIAENANPAAEIVWFQGEVPVSTAP 191
            :......|..|||   :.|   .::|.|    |.:..|:.|||.:|.|.|||..|:....:..:.
Zfish   119 SRTVWLNVQIPSS---QPY---FQLESEEPWVEGEQYTVTCIAPDAKPPAEITIFRDGEELLESE 177

  Fly   192 IVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALPPDVPMRAQVQLSVLYPPGAPFFEG 256
            ..|::.:..|...|.:.:.::.|:.||.:..:|.|::.|.|.  .:...:.:.|.:||.||...|
Zfish   178 SYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASPR--ALETGMTMKVYFPPQAPMIMG 240

  Fly   257 YSQGETLHRGQEVQIACRSRGGNPPAQLTWYRNGVAISSPQRTS--GRLSENVYKFTAAAEDNGA 319
            . :.|.:..|..:::.|.|.||||.|.|.|.:||..:|:.....  .|.:.:|.|.....|||.|
Zfish   241 L-ENEEVKAGSFLKVVCMSYGGNPLATLHWTKNGEVLSTSWDVDFVSRRASSVLKMEVKPEDNHA 304

  Fly   320 NLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGANQAKVGDSVQLSCVTAPSNPQARISWSING 384
            .|.|::.|.:..:|:.....|||.:.|.:|.|.|:.:|..|:.:.|||.|:.|||...|.|.:..
Zfish   305 VLQCQSINQVTRSPMSLTRTLTVPFEPAEVTLLGSFEAVEGEEINLSCSTSSSNPPVHIRWWLGF 369

  Fly   385 RPLDNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPP 449
            :.|:.:........:||.::.||::..:..:.......|.|.|.....:.|.:..:.|.|||...
Zfish   370 KELNRTEVTFAEGDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKV 434

  Fly   450 LLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSK--ITSELSLLVNAS 512
            .|........|.|||.|:|.|.|:||||...|.|.||:|::...||.|.|:  ::.||.|.::.|
Zfish   435 WLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLTLHPS 499

  Fly   513 DNNAIYKCKVQNAAIDIPLFATKTLGVHFPPETVKISVVPKNLVPGIRAKLICDSSSSNPPAKIS 577
            ||.|.|:|...|.|..: |.|.....|.||..:|||....|.|..|....|.|.:.||||.|.||
Zfish   500 DNLATYRCDSSNKAKKV-LSAQTKFRVLFPAVSVKIVAKQKELRRGQTLDLDCLAGSSNPTANIS 563

  Fly   578 WWKDGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGIVYTCQSHNEVLQRSVHETISLDILY 642
            |....:.::|::.|.:...:||...|.::.:.:.........|||:.:.||...|:...|||:|:
Zfish   564 WSLGPVILKGVDQAPKRAEFGGMSRTSKLSLTLEPHHWRKRITCQAFSNVLSEGVNNFYSLDVLF 628

  Fly   643 PPKFETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISD-GPR---- 702
            ||:|...|......:|.....:....|.||..||..|.          ..|::|:.: .||
Zfish   629 PPEFSAEQPDEVQVIEDETATLPAKVSANPDEITCEWI----------FQGEKLVKERDPRYTFS 683

  Fly   703 ---LNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQA 764
               |.|..:||.|.|.||.|..|::|:...::::.|:|:||:...|:......|:.|.|.|...|
Zfish   684 DWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADA 748

  Fly   765 RPLEAAHVRW------SRDGYDLATRTISSFENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQ 823
            .|:.::...|      .||  :|..   .|.::||.:|.|..|.|...|.:.|..||......:.
Zfish   749 NPITSSMFSWKWMGEGERD--ELGE---ESEDDGTGVLTIYEVTRDRAGVYQCTADNGISPTGSV 808

  Fly   824 NVLLVVQTAPEIDHSPGFTRYAAR--LGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSV 886
            ...|:|:.||::...|.:.:.|:|  ....|.::|::...|:..|.|.::|..|.: ...:::..
Zfish   809 EGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDL-GNPRYEET 872

  Fly   887 ERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGV 951
            ......::.....:|..::..||..:.|...||:|:.|..::....:.||.|..|::..|..|.|
Zfish   873 TESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSV 937

  Fly   952 DLNWTPGFDGGMQTYFRLRLKQHGEDKYKYVDAKPGHQNI-SLDGLKPGATYYFSVMAANEAGGS 1015
            .|.|.||||||:...||:|.:..|...|.|||..|....: ::.||.|...|.|||.|.|..|.|
Zfish   938 TLEWMPGFDGGLTQIFRVRYQWAGSASYMYVDVFPPTATVYTVTGLNPSTMYNFSVNALNSIGES 1002

  Fly  1016 KFMPDIK-LTLSKGSQPHSAEYTEKD---------------ELPNVMIIGITSAAMVLLVLN--- 1061
            .:..:.| ||::      :|:..|.|               .|| |.::.|.....||.:||
Zfish  1003 SYADNNKILTVT------TADAKEADGGVSTADQDSALAGGGLP-VYVVVILVVGGVLFLLNPFI 1060

  Fly  1062 AALVAWFVIRRQ---NKSQSEAEPSNDDVYSKDDSQSVYKLPITALQADVQKKAAASTYLVENVD 1123
            ..|:|.:..||.   |.|....|..:::..|...|.:.|      ...::...:|..|.||::..
Zfish  1061 CFLLARWKRRRSLKGNTSSISQETKSEEQRSTASSSTRY------ASRELINASAQRTLLVDSSS 1119

  Fly  1124 IIQSTAYP-------PKYQESSMCTPPYPLCNPDFTRTLPNPKRHSQRNSATGMIEGMQMRSKDD 1181
            ...|:.|.       |.|...:....|....:|:                           ..:|
Zfish  1120 EPDSSVYESYGRQENPHYYYPTEAYSPALYAHPE---------------------------GPED 1157

  Fly  1182 HMLISNGLYIPSPSPASSLVIKGSY--ISSPSPAPPA 1216
                .:|.:..||.......::|.|  :.:.||.|||
Zfish  1158 ----CDGRHGTSPFTHVYEEVRGMYEDLQACSPLPPA 1190

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
hbs	NP_001286439.1	Ig	44..122	CDD:299845	36/77 (47%)
		IG_like	45..137	CDD:214653	38/91 (42%)
		Ig	153..231	CDD:299845	21/81 (26%)
		IG_like	258..342	CDD:214653	26/85 (31%)
		Ig	266..327	CDD:299845	22/62 (35%)
		IG_like	357..>414	CDD:214653	16/56 (29%)
		Ig	360..427	CDD:299845	17/66 (26%)
		Ig	<468..526	CDD:299845	26/59 (44%)
		I-set	547..633	CDD:254352	25/85 (29%)
		Ig	556..631	CDD:299845	21/74 (28%)
		I-set	644..735	CDD:254352	25/98 (26%)
		IGc2	668..725	CDD:197706	20/64 (31%)
		IG_like	751..829	CDD:214653	22/83 (27%)
		IGc2	753..815	CDD:197706	19/67 (28%)
		Ig	852..928	CDD:143165	15/75 (20%)
		FN3	935..1017	CDD:238020	35/82 (43%)
nphs1	NP_001035777.1	IG_like	33..126	CDD:214653	38/92 (41%)
		Ig	133..216	CDD:386229	22/85 (26%)
		Ig	261..313	CDD:386229	18/51 (35%)
		Ig	333..419	CDD:386229	22/85 (26%)
		ig	439..511	CDD:365836	30/71 (42%)
		IG_like	637..719	CDD:214653	22/91 (24%)
		Ig_3	723..800	CDD:372822	22/81 (27%)
		Ig_hNephrin_like	814..922	CDD:143250	21/108 (19%)
		FN3	921..1012	CDD:238020	36/90 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170576440
Domainoid	1	1.000	94	1.000	Domainoid score	I7422
eggNOG	1	0.900	-	-		E1_KOG3515
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	533	1.000	Inparanoid score	I1173
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D141865at33208
OrthoFinder	1	1.000	-	-		FOG0005450
OrthoInspector	1	1.000	-	-		otm26237
orthoMCL	1	0.900	-	-		OOG6_107508
Panther	1	1.100	-	-	O	PTHR11640
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X3304
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	14	13.760