DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Rgl and rasgrf1

DIOPT Version :9

Sequence 1:	NP_001261814.1	Gene:	Rgl / 44115	FlyBaseID:	FBgn0026376	Length:	973	Species:	Drosophila melanogaster
Sequence 2:	XP_009291904.1	Gene:	rasgrf1 / 568885	ZFINID:	ZDB-GENE-090311-28	Length:	1263	Species:	Danio rerio

Alignment Length:	681	Identity:	152/681 - (22%)
Similarity:	236/681 - (34%)	Gaps:	227/681 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly   223 NDSDDEISHLEWETVRVRFVKAATLARLVEALATDDGELESTFINVFLSTYRTFSTPKQVLSLLT 287
            :|.|...|.: ..:.:|..::.|::.||:|.| ||...|...|:|.||.:||.|::...||..|.
Zfish   626 DDVDIRFSKM-MNSCKVLQIRYASVERLLERL-TDLRFLSIDFLNTFLHSYRVFTSADVVLDKLI 688

  Fly   288 QRY----DALHEKHLEELEQAQQNGQVM--DPAYDPHA----------------SIHEQHKKTL- 329
            ..|    .|:..:.||....:.||.:::  :|...|.|                |.:.:.|.:|
Zfish   689 TIYKRPISAIPARSLELFFASSQNNKLLYGEPPKSPRASRKFSSPPPLAITKTSSPNRRRKLSLN 753

  Fly   330 -----------VSALHVWLDGFP------------------------------------------ 341
                       ::||....:|:.
Zfish   754 IPIITGGKALDLAALSCSSNGYASMYSTMSPFSKTTLDINKLYVSSPITSKIPDEGEGKTDKADE 818

  Fly   342 -----------EDWHEDNLQQILAFATKRLKRSDL---HIKVLNRLERLIKQSLYGNGGGSGGSE 392
                       |:...|.:|...|.|.....:|..   ::|..|..|    .||:....|...|.
Zfish   819 ISLCKQDNSVREESDNDQIQSDEAEAEVSPTKSPTTPKNVKCKNSSE----FSLFSYNNGMVMSS 879

  Fly   393 ----NNGHSWLSAARSQQMQQFMIPT----------------HYGSSYDLTDQFNG--------- 428
                :|..|.||||.:     |.|.|                ...|:...|||.||
Zfish   880 CRELDNNRSALSAASA-----FAIATAGANEGTPTKEKYRRMSLASTGFPTDQRNGDKEFVIRRA 939

  Fly   429 -------------------------------MYLSPMGHGP------------IYRGPT------ 444
                                           .:|..:.|.|            |.|..|
Zfish   940 ATNRVLNVLRHWVSKHSQDFETNTELKMKVISFLEEVMHDPELLTQERKAAANIIRTLTQEDPGD 1004

  Fly   445 ------HFLQ------AFRFPHVPVRHFAEQLTRMDTELFKRLIPH-QCLGHTWARRDSG-GSET 495
                  ..||      :..|.:......|||||.:|..:|| :||: :..|..|.:.|.. .:..
Zfish  1005 NQICLEEVLQMAEGGKSESFENHSALEIAEQLTLLDHLVFK-VIPYEEFFGQGWMKNDKNEKTPY 1068

  Fly   496 VVATINQFNAVLFRVVSSILIDRLKP-QERALNISRWIDIAQELRMLKNFSSLKAIISALNSNSI 559
            ::.|...||.:...:.:.||  |.:. ..|...:.:|:.:|...|.|.|::::..|.|:||.:||
Zfish  1069 IMKTTKHFNDISDLIATEIL--RCEDVNVRVAVMEKWVAVADICRCLHNYNAVLEITSSLNRSSI 1131

  Fly   560 YRLSKIWEVLPKERMEVFTELANICSEDNNAWTLREVLKREGTAKNPDPGSDHSDRHLQKLILNL 624
            :||.|.|..:.|:...|..:|..:.|.:.....|||.|      ||.||
Zfish  1132 FRLKKTWLKVSKQTKTVIDKLQKLVSSEGRFKNLREAL------KNCDP---------------- 1174

  Fly   625 GTQTSHGTIPYLGTFLTDLTMIHTANPDYLTENKLINFDKKRKEFEVLAQIKLLQGAANTYNLQA 689
                  ..:||||.:||||..|....|:| ||:.|:||.|.|....::.:|:..|..|...:.|.
Zfish  1175 ------PCVPYLGMYLTDLAFIEEGTPNY-TEDNLVNFSKMRMISHIIREIRQFQQTAYKIDYQP 1232

  Fly   690 DALFDHWFNSMPVFDEREAFELSCRLEPPPP 720
            .|.. :..:...|.:|...:|.|.|:||..|
Zfish  1233 KAAL-YLLDRSAVMEEEGLYEASLRIEPKVP 1262

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Rgl	NP_001261814.1	REM	246..378	CDD:100121	41/221 (19%)
		RasGEF	454..719	CDD:214539	77/267 (29%)
		RalGDS_RA	858..946	CDD:176351
rasgrf1	XP_009291904.1	PH_RasGRF1_2	19..154	CDD:270081
		PH	24..129	CDD:278594
		RhoGEF	244..425	CDD:214619
		PH-like	474..596	CDD:302622
		PH	489..592	CDD:278594
		RasGEF_N	643..>691	CDD:279012	18/48 (38%)
		REM	<928..997	CDD:295342	7/68 (10%)
		RasGEF	1024..1261	CDD:214539	78/269 (29%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910