DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Rgl and rapgef5a

DIOPT Version :9

Sequence 1:	NP_001261814.1	Gene:	Rgl / 44115	FlyBaseID:	FBgn0026376	Length:	973	Species:	Drosophila melanogaster
Sequence 2:	XP_002665506.2	Gene:	rapgef5a / 100331732	ZFINID:	ZDB-GENE-030131-7681	Length:	615	Species:	Danio rerio

Alignment Length:	657	Identity:	126/657 - (19%)
Similarity:	221/657 - (33%)	Gaps:	215/657 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   186 TYTKPTWRLWGEEHEKNAIFTVYLKKVRYHRPTPTASNDSDDEISHLEWETVRVRF-VKAATLAR 249
            |:..|.:|...::.::.......:.:..:.....|.|:..|:..|......:..|: |.:.|..:
Zfish    47 TFDIPHFRFIDDDEDEEEEDDYVMNRHCWSSSRSTCSSSEDEYPSDSSSSALSCRYVVVSGTPHK 111

  Fly   250 LVEALATD----------DGELESTFINVFLSTYRTFSTPKQVLSLLTQRY------------DA 292
            ::|.|.:|          :|:.....::.||.||..|.:..::...|...|            :|
Zfish   112 ILEHLLSDLRLDEHQGATEGKEAEMLLDDFLLTYLVFMSTSELCQALLGHYCTKRCRGKEEGNEA 176

  Fly   293 LH-----------------------------------------------EKHLEELEQAQQNGQV 310
            |:                                               ||.|:|.::..:....
Zfish   177 LYRKRKVLHLVSQWSSLYKDFLREEENVKLFMKTLYRYVLEDVYEFPTLEKDLKEFQKLLRRRHT 241

  Fly   311 MDPAYDPHASIHEQHKKTLVSAL-----HVWLDGFPEDWHE---------DN-----------LQ 350
            :|. |.||     |..|.|...|     .|.|...|.:..:         |:           .|
Zfish   242 VDD-YSPH-----QKNKALYQQLSLKENSVSLRASPVESRDVMCRVYVSCDSYLSMRVKPAVVAQ 300

  Fly   351 QILAFATKRLKR--SDLHI---------KVLNRLERLIKQSLYGNGGGSGGSENNGHSWLSAARS 404
            ::|...::|:.|  .|:.:         :||...:.:..:||...|.            |.|.|
Zfish   301 ELLHIVSQRMDRCEDDMMLLAQTYSGEKRVLQPHDSIYSESLVAPGR------------LIACR- 352

  Fly   405 QQMQQFMIPTHYGSSYDLTDQFNGMYLSPMGHGPIYRGPTHFLQAFRFPHVPVRHFAEQLTRMDT 469
            :.:.:.:.|        ||:      ...:|..|:           |...:.....|..||..|.
Zfish   353 RDLSEILPP--------LTE------CPDLGQKPV-----------RLLGINTWDVAVALTNFDW 392

  Fly   470 ELFKRLIPHQCLGHTWARRDSGGSETVVA-TINQFNAVLFRVVSSILIDRLKP--QERALNISRW 531
            .||..:...:.:.:|::|:.|.|....:. .:.:.|.|...|:|.:|   |.|  .:|...:.::
Zfish   393 NLFNSIHEQELIFYTFSRQASSGHTVALEFLLQRCNEVQQWVMSEVL---LCPSLSKRVQLLKKF 454

  Fly   532 IDIAQELRMLKNFSSLKAIISALNSNSIYRLSKIWEVLPKERMEVFTELANICSEDNNAWTLREV 596
            |.||...:..:|.:|..|||..||:.::.||::.||.:|.:..::|:||..:.....|....|:.
Zfish   455 IKIAAHCKAQRNLNSSFAIIMGLNTAAVSRLNQTWEKVPGKFKKLFSELELLTDPSMNHKAYRDA 519

  Fly   597 LKREGTAKNPDPGSDHSDRHLQKLILNLGTQTSHGTIPYLGTFLTDLTMIHTANPDYLTENKLIN 661
            .|:....|                            ||::...|.|:|.||..|..:  .:.|:|
Zfish   520 FKKMKPPK----------------------------IPFMPLLLKDITFIHEGNKTF--HDNLVN 554

  Fly   662 FDKKRKEFEVLAQIKLLQGAANTYNLQADALFDHWFNSMPVFDEREA----------------FE 710
            |:|             |...|:|..|......|...|.:...|..|.                ||
Zfish   555 FEK-------------LHMIADTVRLIRHCQMDQTGNELSAVDSAEVRSSVHYLHIIDNQQTLFE 606

  Fly   711 LSCRLEP 717
            ||.||||
Zfish   607 LSHRLEP 613

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Rgl	NP_001261814.1	REM	246..378	CDD:100121	37/236 (16%)
		RasGEF	454..719	CDD:214539	68/283 (24%)
		RalGDS_RA	858..946	CDD:176351
rapgef5a	XP_002665506.2	RasGEFN	100..237	CDD:214571	20/136 (15%)
rapgef5a	XP_002665506.2	RasGEF	375..615	CDD:214539	68/285 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3629
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810